Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils
Background : Mongolian gerbils have been reported to be a suitable model for Helicobacter pylori‐associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and H. pylori infection...
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| Veröffentlicht in: | Journal of gastroenterology and hepatology 1999-11, Vol.14 (11), p.1062-1069 |
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| container_title | Journal of gastroenterology and hepatology |
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| creator | Suzuki, Hidekazu Mori, Mikiji Seto, Koichi Nagahashi, Shoichi Kawaguchi, Chizuko Kai, Akemi Akiba, Yasutada Suzuki, Masayuki Suematsu, Makoto Miura, Soichiro Ishii, Hiromasa |
| description | Background
: Mongolian gerbils have been reported to be a suitable model for Helicobacter pylori‐associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and H. pylori infection remains unknown. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury.
Methods
: Male Mongolian gerbils were used for the study. Helicobacter pylori was orally inoculated after 15 h fasting (Hp group). Thirty minutes prior to H. pylori inoculation, a group of gerbils was orally treated with 40% ethanol (20 mL/kg; E + Hp group). Another group of animals was treated either with H. pylori culture media alone (controls) or with 40% ethanol plus culture media (E group). Gerbils were killed 2, 4 or 12 weeks after H. pylori inoculation. Helicobacter pylori infection was confirmed by both histological examination and serological tests. Mucosal damage was evaluated histologically according to the modified Sydney system.
Results
: Although in the controls and E group no significant change to the gastric mucose was observed, persistent H. pylori infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Hp and E + Hp groups after 4 weeks. The histological scores for polymorphonuclear cell infiltration and myeloperoxidase activity were higher in the E + Hp group at 4 weeks than in the Hp group (P < 0.05).
Conclusions
: Ethanol intake preceding H. pylori inoculation could promote the progression of gastric mucosal inflammation in Mongolian gerbils.
© 1999 Blackwell Science Asia Pty Ltd |
| doi_str_mv | 10.1046/j.1440-1746.1999.01999.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69302611</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69302611</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4989-ccd1d92f4db7d400f96d5b2e1d1746ba34c1892b320e3281ea68b84e7bf614ef3</originalsourceid><addsrcrecordid>eNqNkM1u1DAURi0EokPhFZAXiF0G38T58YIFKu0E1MKmiKVlOzeDp0482ImYefsmzajtko1tyee7n30IocDWwHjxabcGzlkCJS_WIIRYs4f18IKsHi9ekhWrIE9EBuKMvIlxxxjjrMxfkzNgeckhS1ckXg5_VO8dtf2g7pDuAxpsbL-lNTprvFZmwED3R-eDnSBvRqcG6_uJ9J0fMNKtikOwhnaj8VHNk1qnum6hbE9vfL_1zqqebjFo6-Jb8qpVLuK7035Ofl1d3l7UyfXPzbeLL9eJ4aISiTENNCJteaPLhjPWiqLJdYrQzN_TKuMGKpHqLGWYpRWgKipdcSx1WwDHNjsnH5e501P_jhgH2dlo0DnVox-jLETG0gJgAqsFNMHHGLCV-2A7FY4SmJyFy52cvcq5WM6q5YNweZii708do-6weRZcDE_AhxOgolGuDao3Nj5xUIFIywn7vGD_rMPjf_fL75t6Pk35ZMnbOODhMa_CnSzKrMzl7x8b-ZUX7Oa2rmWa3QMpuK20</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69302611</pqid></control><display><type>article</type><title>Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils</title><source>MEDLINE</source><source>Wiley Online Library</source><creator>Suzuki, Hidekazu ; Mori, Mikiji ; Seto, Koichi ; Nagahashi, Shoichi ; Kawaguchi, Chizuko ; Kai, Akemi ; Akiba, Yasutada ; Suzuki, Masayuki ; Suematsu, Makoto ; Miura, Soichiro ; Ishii, Hiromasa</creator><creatorcontrib>Suzuki, Hidekazu ; Mori, Mikiji ; Seto, Koichi ; Nagahashi, Shoichi ; Kawaguchi, Chizuko ; Kai, Akemi ; Akiba, Yasutada ; Suzuki, Masayuki ; Suematsu, Makoto ; Miura, Soichiro ; Ishii, Hiromasa</creatorcontrib><description>Background
: Mongolian gerbils have been reported to be a suitable model for Helicobacter pylori‐associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and H. pylori infection remains unknown. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury.
Methods
: Male Mongolian gerbils were used for the study. Helicobacter pylori was orally inoculated after 15 h fasting (Hp group). Thirty minutes prior to H. pylori inoculation, a group of gerbils was orally treated with 40% ethanol (20 mL/kg; E + Hp group). Another group of animals was treated either with H. pylori culture media alone (controls) or with 40% ethanol plus culture media (E group). Gerbils were killed 2, 4 or 12 weeks after H. pylori inoculation. Helicobacter pylori infection was confirmed by both histological examination and serological tests. Mucosal damage was evaluated histologically according to the modified Sydney system.
Results
: Although in the controls and E group no significant change to the gastric mucose was observed, persistent H. pylori infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Hp and E + Hp groups after 4 weeks. The histological scores for polymorphonuclear cell infiltration and myeloperoxidase activity were higher in the E + Hp group at 4 weeks than in the Hp group (P < 0.05).
Conclusions
: Ethanol intake preceding H. pylori inoculation could promote the progression of gastric mucosal inflammation in Mongolian gerbils.
© 1999 Blackwell Science Asia Pty Ltd</description><identifier>ISSN: 0815-9319</identifier><identifier>EISSN: 1440-1746</identifier><identifier>DOI: 10.1046/j.1440-1746.1999.01999.x</identifier><identifier>PMID: 10574132</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Science Pty</publisher><subject>acute gastric mucosal lesions ; alcohol ; Alcohol Drinking - adverse effects ; Alcohol Drinking - pathology ; Animals ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Biological and medical sciences ; Disease Models, Animal ; Gastric Mucosa - pathology ; gastritis ; Gastritis - pathology ; Gerbillinae ; Helicobacter Infections - pathology ; Helicobacter pylori ; Human bacterial diseases ; Infectious diseases ; leucocyte infiltration ; Male ; Medical sciences ; Neutrophils - pathology</subject><ispartof>Journal of gastroenterology and hepatology, 1999-11, Vol.14 (11), p.1062-1069</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4989-ccd1d92f4db7d400f96d5b2e1d1746ba34c1892b320e3281ea68b84e7bf614ef3</citedby><cites>FETCH-LOGICAL-c4989-ccd1d92f4db7d400f96d5b2e1d1746ba34c1892b320e3281ea68b84e7bf614ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1440-1746.1999.01999.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1440-1746.1999.01999.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1181927$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10574132$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Hidekazu</creatorcontrib><creatorcontrib>Mori, Mikiji</creatorcontrib><creatorcontrib>Seto, Koichi</creatorcontrib><creatorcontrib>Nagahashi, Shoichi</creatorcontrib><creatorcontrib>Kawaguchi, Chizuko</creatorcontrib><creatorcontrib>Kai, Akemi</creatorcontrib><creatorcontrib>Akiba, Yasutada</creatorcontrib><creatorcontrib>Suzuki, Masayuki</creatorcontrib><creatorcontrib>Suematsu, Makoto</creatorcontrib><creatorcontrib>Miura, Soichiro</creatorcontrib><creatorcontrib>Ishii, Hiromasa</creatorcontrib><title>Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils</title><title>Journal of gastroenterology and hepatology</title><addtitle>J Gastroenterol Hepatol</addtitle><description>Background
: Mongolian gerbils have been reported to be a suitable model for Helicobacter pylori‐associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and H. pylori infection remains unknown. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury.
Methods
: Male Mongolian gerbils were used for the study. Helicobacter pylori was orally inoculated after 15 h fasting (Hp group). Thirty minutes prior to H. pylori inoculation, a group of gerbils was orally treated with 40% ethanol (20 mL/kg; E + Hp group). Another group of animals was treated either with H. pylori culture media alone (controls) or with 40% ethanol plus culture media (E group). Gerbils were killed 2, 4 or 12 weeks after H. pylori inoculation. Helicobacter pylori infection was confirmed by both histological examination and serological tests. Mucosal damage was evaluated histologically according to the modified Sydney system.
Results
: Although in the controls and E group no significant change to the gastric mucose was observed, persistent H. pylori infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Hp and E + Hp groups after 4 weeks. The histological scores for polymorphonuclear cell infiltration and myeloperoxidase activity were higher in the E + Hp group at 4 weeks than in the Hp group (P < 0.05).
Conclusions
: Ethanol intake preceding H. pylori inoculation could promote the progression of gastric mucosal inflammation in Mongolian gerbils.
© 1999 Blackwell Science Asia Pty Ltd</description><subject>acute gastric mucosal lesions</subject><subject>alcohol</subject><subject>Alcohol Drinking - adverse effects</subject><subject>Alcohol Drinking - pathology</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Biological and medical sciences</subject><subject>Disease Models, Animal</subject><subject>Gastric Mucosa - pathology</subject><subject>gastritis</subject><subject>Gastritis - pathology</subject><subject>Gerbillinae</subject><subject>Helicobacter Infections - pathology</subject><subject>Helicobacter pylori</subject><subject>Human bacterial diseases</subject><subject>Infectious diseases</subject><subject>leucocyte infiltration</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neutrophils - pathology</subject><issn>0815-9319</issn><issn>1440-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1u1DAURi0EokPhFZAXiF0G38T58YIFKu0E1MKmiKVlOzeDp0482ImYefsmzajtko1tyee7n30IocDWwHjxabcGzlkCJS_WIIRYs4f18IKsHi9ekhWrIE9EBuKMvIlxxxjjrMxfkzNgeckhS1ckXg5_VO8dtf2g7pDuAxpsbL-lNTprvFZmwED3R-eDnSBvRqcG6_uJ9J0fMNKtikOwhnaj8VHNk1qnum6hbE9vfL_1zqqebjFo6-Jb8qpVLuK7035Ofl1d3l7UyfXPzbeLL9eJ4aISiTENNCJteaPLhjPWiqLJdYrQzN_TKuMGKpHqLGWYpRWgKipdcSx1WwDHNjsnH5e501P_jhgH2dlo0DnVox-jLETG0gJgAqsFNMHHGLCV-2A7FY4SmJyFy52cvcq5WM6q5YNweZii708do-6weRZcDE_AhxOgolGuDao3Nj5xUIFIywn7vGD_rMPjf_fL75t6Pk35ZMnbOODhMa_CnSzKrMzl7x8b-ZUX7Oa2rmWa3QMpuK20</recordid><startdate>199911</startdate><enddate>199911</enddate><creator>Suzuki, Hidekazu</creator><creator>Mori, Mikiji</creator><creator>Seto, Koichi</creator><creator>Nagahashi, Shoichi</creator><creator>Kawaguchi, Chizuko</creator><creator>Kai, Akemi</creator><creator>Akiba, Yasutada</creator><creator>Suzuki, Masayuki</creator><creator>Suematsu, Makoto</creator><creator>Miura, Soichiro</creator><creator>Ishii, Hiromasa</creator><general>Blackwell Science Pty</general><general>Blackwell Science</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199911</creationdate><title>Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils</title><author>Suzuki, Hidekazu ; Mori, Mikiji ; Seto, Koichi ; Nagahashi, Shoichi ; Kawaguchi, Chizuko ; Kai, Akemi ; Akiba, Yasutada ; Suzuki, Masayuki ; Suematsu, Makoto ; Miura, Soichiro ; Ishii, Hiromasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4989-ccd1d92f4db7d400f96d5b2e1d1746ba34c1892b320e3281ea68b84e7bf614ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>acute gastric mucosal lesions</topic><topic>alcohol</topic><topic>Alcohol Drinking - adverse effects</topic><topic>Alcohol Drinking - pathology</topic><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Biological and medical sciences</topic><topic>Disease Models, Animal</topic><topic>Gastric Mucosa - pathology</topic><topic>gastritis</topic><topic>Gastritis - pathology</topic><topic>Gerbillinae</topic><topic>Helicobacter Infections - pathology</topic><topic>Helicobacter pylori</topic><topic>Human bacterial diseases</topic><topic>Infectious diseases</topic><topic>leucocyte infiltration</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neutrophils - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Hidekazu</creatorcontrib><creatorcontrib>Mori, Mikiji</creatorcontrib><creatorcontrib>Seto, Koichi</creatorcontrib><creatorcontrib>Nagahashi, Shoichi</creatorcontrib><creatorcontrib>Kawaguchi, Chizuko</creatorcontrib><creatorcontrib>Kai, Akemi</creatorcontrib><creatorcontrib>Akiba, Yasutada</creatorcontrib><creatorcontrib>Suzuki, Masayuki</creatorcontrib><creatorcontrib>Suematsu, Makoto</creatorcontrib><creatorcontrib>Miura, Soichiro</creatorcontrib><creatorcontrib>Ishii, Hiromasa</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of gastroenterology and hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Hidekazu</au><au>Mori, Mikiji</au><au>Seto, Koichi</au><au>Nagahashi, Shoichi</au><au>Kawaguchi, Chizuko</au><au>Kai, Akemi</au><au>Akiba, Yasutada</au><au>Suzuki, Masayuki</au><au>Suematsu, Makoto</au><au>Miura, Soichiro</au><au>Ishii, Hiromasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils</atitle><jtitle>Journal of gastroenterology and hepatology</jtitle><addtitle>J Gastroenterol Hepatol</addtitle><date>1999-11</date><risdate>1999</risdate><volume>14</volume><issue>11</issue><spage>1062</spage><epage>1069</epage><pages>1062-1069</pages><issn>0815-9319</issn><eissn>1440-1746</eissn><abstract>Background
: Mongolian gerbils have been reported to be a suitable model for Helicobacter pylori‐associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and H. pylori infection remains unknown. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury.
Methods
: Male Mongolian gerbils were used for the study. Helicobacter pylori was orally inoculated after 15 h fasting (Hp group). Thirty minutes prior to H. pylori inoculation, a group of gerbils was orally treated with 40% ethanol (20 mL/kg; E + Hp group). Another group of animals was treated either with H. pylori culture media alone (controls) or with 40% ethanol plus culture media (E group). Gerbils were killed 2, 4 or 12 weeks after H. pylori inoculation. Helicobacter pylori infection was confirmed by both histological examination and serological tests. Mucosal damage was evaluated histologically according to the modified Sydney system.
Results
: Although in the controls and E group no significant change to the gastric mucose was observed, persistent H. pylori infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Hp and E + Hp groups after 4 weeks. The histological scores for polymorphonuclear cell infiltration and myeloperoxidase activity were higher in the E + Hp group at 4 weeks than in the Hp group (P < 0.05).
Conclusions
: Ethanol intake preceding H. pylori inoculation could promote the progression of gastric mucosal inflammation in Mongolian gerbils.
© 1999 Blackwell Science Asia Pty Ltd</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Science Pty</pub><pmid>10574132</pmid><doi>10.1046/j.1440-1746.1999.01999.x</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library |
| subjects | acute gastric mucosal lesions alcohol Alcohol Drinking - adverse effects Alcohol Drinking - pathology Animals Bacterial diseases Bacterial diseases of the digestive system and abdomen Biological and medical sciences Disease Models, Animal Gastric Mucosa - pathology gastritis Gastritis - pathology Gerbillinae Helicobacter Infections - pathology Helicobacter pylori Human bacterial diseases Infectious diseases leucocyte infiltration Male Medical sciences Neutrophils - pathology |
| title | Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils |
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