Phage DNA transport across membranes
Phage nucleic acid transport is atypical in bacterial membrane transport: it is unidirectional and concerns a unique molecule the size of which may represent 50 times that of the bacterium. The rate of DNA transport, although it varies from one phage to another, can reach values as high as 3 000 bp...
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| Veröffentlicht in: | Research in microbiology 1999-10, Vol.150 (8), p.499-505 |
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| container_title | Research in microbiology |
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| creator | Letellier, Lucienne Plançon, Laure Bonhivers, Mélanie Boulanger, Pascale |
| description | Phage nucleic acid transport is atypical in bacterial membrane transport: it is unidirectional and concerns a unique molecule the size of which may represent 50 times that of the bacterium. The rate of DNA transport, although it varies from one phage to another, can reach values as high as 3 000 bp s
–1. This raises the following questions which will be discussed in this review. Is there a single mechanism of transport for all types of phages? Does the phage genome cross the outer and inner membranes by a unique mechanism? Is it transported as a free molecule or in association with proteins? How does it avoid periplasmic nucleases? Is such transport dependent on phage and/or host cell components? What is the driving force for transport? Recent cryoelectron microscopy experiments will be presented which show that it is possible to encapsulate a phage genome (121 000 bp) into unilamellar liposomes. The interest of such a model system in gene delivery and in the study of the mechanisms of DNA compaction will be discussed. |
| doi_str_mv | 10.1016/S0923-2508(99)00107-2 |
| format | Article |
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–1. This raises the following questions which will be discussed in this review. Is there a single mechanism of transport for all types of phages? Does the phage genome cross the outer and inner membranes by a unique mechanism? Is it transported as a free molecule or in association with proteins? How does it avoid periplasmic nucleases? Is such transport dependent on phage and/or host cell components? What is the driving force for transport? Recent cryoelectron microscopy experiments will be presented which show that it is possible to encapsulate a phage genome (121 000 bp) into unilamellar liposomes. The interest of such a model system in gene delivery and in the study of the mechanisms of DNA compaction will be discussed.</description><identifier>ISSN: 0923-2508</identifier><identifier>EISSN: 1769-7123</identifier><identifier>DOI: 10.1016/S0923-2508(99)00107-2</identifier><identifier>PMID: 10577483</identifier><language>eng</language><publisher>Paris: Elsevier SAS</publisher><subject>Bacterial Outer Membrane Proteins - chemistry ; bacteriophage ; Biological and medical sciences ; Biological Transport - physiology ; Cell Membrane - metabolism ; DNA compaction ; DNA transport ; DNA, Viral - metabolism ; envelope ; Escherichia coli Proteins ; Fundamental and applied biological sciences. Psychology ; gene delivery ; Genetics ; liposomes ; Liposomes - chemistry ; Liposomes - ultrastructure ; Microbiology ; outer membrane receptor ; Peptidoglycan - metabolism ; Proton-Motive Force - physiology ; Receptors, Virus - chemistry ; T-Phages - metabolism ; T-Phages - ultrastructure ; Virology</subject><ispartof>Research in microbiology, 1999-10, Vol.150 (8), p.499-505</ispartof><rights>1999 Éditions scientifiques et médicales Elsevier SAS</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-955405def2f3c14bd69807d6b65ad6d6102a47c3bd0bfbe7be236d0ee13ae48f3</citedby><cites>FETCH-LOGICAL-c400t-955405def2f3c14bd69807d6b65ad6d6102a47c3bd0bfbe7be236d0ee13ae48f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0923250899001072$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1184282$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10577483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Letellier, Lucienne</creatorcontrib><creatorcontrib>Plançon, Laure</creatorcontrib><creatorcontrib>Bonhivers, Mélanie</creatorcontrib><creatorcontrib>Boulanger, Pascale</creatorcontrib><title>Phage DNA transport across membranes</title><title>Research in microbiology</title><addtitle>Res Microbiol</addtitle><description>Phage nucleic acid transport is atypical in bacterial membrane transport: it is unidirectional and concerns a unique molecule the size of which may represent 50 times that of the bacterium. The rate of DNA transport, although it varies from one phage to another, can reach values as high as 3 000 bp s
–1. This raises the following questions which will be discussed in this review. Is there a single mechanism of transport for all types of phages? Does the phage genome cross the outer and inner membranes by a unique mechanism? Is it transported as a free molecule or in association with proteins? How does it avoid periplasmic nucleases? Is such transport dependent on phage and/or host cell components? What is the driving force for transport? Recent cryoelectron microscopy experiments will be presented which show that it is possible to encapsulate a phage genome (121 000 bp) into unilamellar liposomes. The interest of such a model system in gene delivery and in the study of the mechanisms of DNA compaction will be discussed.</description><subject>Bacterial Outer Membrane Proteins - chemistry</subject><subject>bacteriophage</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - physiology</subject><subject>Cell Membrane - metabolism</subject><subject>DNA compaction</subject><subject>DNA transport</subject><subject>DNA, Viral - metabolism</subject><subject>envelope</subject><subject>Escherichia coli Proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene delivery</subject><subject>Genetics</subject><subject>liposomes</subject><subject>Liposomes - chemistry</subject><subject>Liposomes - ultrastructure</subject><subject>Microbiology</subject><subject>outer membrane receptor</subject><subject>Peptidoglycan - metabolism</subject><subject>Proton-Motive Force - physiology</subject><subject>Receptors, Virus - chemistry</subject><subject>T-Phages - metabolism</subject><subject>T-Phages - ultrastructure</subject><subject>Virology</subject><issn>0923-2508</issn><issn>1769-7123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlKBDEQhoMoOi6PoMxBRA-tlb1zksEdRAX1HNJJtbZM22PSI_j2ZhbUm6cU4auqvz5CdikcU6Dq5BEM4wWTUB4acwRAQRdshQyoVqbQlPFVMvhBNshmSm8ZklqLdbJBYVaUfED2H17dCw7P70bDPrr3NOliP3Q-dikNW2yr_Idpm6zVbpxwZ_lukefLi6ez6-L2_urmbHRbeAHQF0ZKATJgzWruqaiCMiXooColXVBBUWBOaM-rAFVdoa6QcRUAkXKHoqz5FjlYzJ3E7mOKqbdtkzyOxzlEN01WGQ5M5Nz_gVQLIbmUGZQLcH5RxNpOYtO6-GUp2JlHO_doZ5KsMXbu0bLct7dcMK1aDH-6FuIysL8EXPJuXGdPvkm_HC0FK2dzThcYZm2fDUabfIPvHkMT0fc2dM0_Sb4BOPuNKw</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Letellier, Lucienne</creator><creator>Plançon, Laure</creator><creator>Bonhivers, Mélanie</creator><creator>Boulanger, Pascale</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Phage DNA transport across membranes</title><author>Letellier, Lucienne ; Plançon, Laure ; Bonhivers, Mélanie ; Boulanger, Pascale</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-955405def2f3c14bd69807d6b65ad6d6102a47c3bd0bfbe7be236d0ee13ae48f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Bacterial Outer Membrane Proteins - chemistry</topic><topic>bacteriophage</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - physiology</topic><topic>Cell Membrane - metabolism</topic><topic>DNA compaction</topic><topic>DNA transport</topic><topic>DNA, Viral - metabolism</topic><topic>envelope</topic><topic>Escherichia coli Proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene delivery</topic><topic>Genetics</topic><topic>liposomes</topic><topic>Liposomes - chemistry</topic><topic>Liposomes - ultrastructure</topic><topic>Microbiology</topic><topic>outer membrane receptor</topic><topic>Peptidoglycan - metabolism</topic><topic>Proton-Motive Force - physiology</topic><topic>Receptors, Virus - chemistry</topic><topic>T-Phages - metabolism</topic><topic>T-Phages - ultrastructure</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Letellier, Lucienne</creatorcontrib><creatorcontrib>Plançon, Laure</creatorcontrib><creatorcontrib>Bonhivers, Mélanie</creatorcontrib><creatorcontrib>Boulanger, Pascale</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Letellier, Lucienne</au><au>Plançon, Laure</au><au>Bonhivers, Mélanie</au><au>Boulanger, Pascale</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phage DNA transport across membranes</atitle><jtitle>Research in microbiology</jtitle><addtitle>Res Microbiol</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>150</volume><issue>8</issue><spage>499</spage><epage>505</epage><pages>499-505</pages><issn>0923-2508</issn><eissn>1769-7123</eissn><abstract>Phage nucleic acid transport is atypical in bacterial membrane transport: it is unidirectional and concerns a unique molecule the size of which may represent 50 times that of the bacterium. The rate of DNA transport, although it varies from one phage to another, can reach values as high as 3 000 bp s
–1. This raises the following questions which will be discussed in this review. Is there a single mechanism of transport for all types of phages? Does the phage genome cross the outer and inner membranes by a unique mechanism? Is it transported as a free molecule or in association with proteins? How does it avoid periplasmic nucleases? Is such transport dependent on phage and/or host cell components? What is the driving force for transport? Recent cryoelectron microscopy experiments will be presented which show that it is possible to encapsulate a phage genome (121 000 bp) into unilamellar liposomes. The interest of such a model system in gene delivery and in the study of the mechanisms of DNA compaction will be discussed.</abstract><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>10577483</pmid><doi>10.1016/S0923-2508(99)00107-2</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| subjects | Bacterial Outer Membrane Proteins - chemistry bacteriophage Biological and medical sciences Biological Transport - physiology Cell Membrane - metabolism DNA compaction DNA transport DNA, Viral - metabolism envelope Escherichia coli Proteins Fundamental and applied biological sciences. Psychology gene delivery Genetics liposomes Liposomes - chemistry Liposomes - ultrastructure Microbiology outer membrane receptor Peptidoglycan - metabolism Proton-Motive Force - physiology Receptors, Virus - chemistry T-Phages - metabolism T-Phages - ultrastructure Virology |
| title | Phage DNA transport across membranes |
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