Development of a bifunctional sensor using haptenized acetylcholinesterase and application for the detection of cocaine and organophosphates
We developed a dual piezoelectric/amperometric sensor for the detection of two unrelated analytes in one experiment that uses propidium to anchor acetylcholinesterases (AChE) at the surface. This mass-sensitive sensor does not only allow the examination of the interaction between AChE and the modifi...
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| Veröffentlicht in: | Biosensors & bioelectronics 2008-09, Vol.24 (1), p.111-117 |
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| container_title | Biosensors & bioelectronics |
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| creator | Teller, Carsten Halámek, Jan Žeravík, Jiři Stöcklein, Walter F.M. Scheller, Frieder W. |
| description | We developed a dual piezoelectric/amperometric sensor for the detection of two unrelated analytes in one experiment that uses propidium to anchor acetylcholinesterases (AChE) at the surface. This mass-sensitive sensor does not only allow the examination of the interaction between AChE and the modified surface but also the detection of in situ inhibition of the surface-bound AChE.
Here we describe the application of the propidium-based sensor in combination with a modified AChE. For this reason the cocaine derivative benzoylecgonine (BZE) was coupled via a 10
Å long hydrophilic linker – 1,8-diamino-3,4-dioxaoctane – to carboxylic groups of the AChE after EDC/NHS activation. Thus the modified AChE (BZE–AChE) possesses an additional recognition element besides the inhibitor binding site. After the deposition of BZE–AChE on the sensor surface the binding of an anti-BZE-antibody to the BZE–AChE can be monitored. This makes it possible to determine two analytes – cocaine and organophosphate – in one experiment by measuring antibody binding and decrease in enzymatic activity, respectively. Furthermore it was also shown that other cocaine-binding enzymes, e.g., butyrylcholinesterase, can bind to the modified BZE–AChE.
The competitive immunoassay allowed the detection of cocaine with a dynamic range from
1
0
−
9
to
1
0
−
7
M. The organophosphate chlorpyrifos-oxon could be detected in concentrations from
1
0
−
6
down to
1
0
−
8
M after 20
min of injection time (equals to 500
μ
L sample volume. |
| doi_str_mv | 10.1016/j.bios.2008.03.027 |
| format | Article |
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Here we describe the application of the propidium-based sensor in combination with a modified AChE. For this reason the cocaine derivative benzoylecgonine (BZE) was coupled via a 10
Å long hydrophilic linker – 1,8-diamino-3,4-dioxaoctane – to carboxylic groups of the AChE after EDC/NHS activation. Thus the modified AChE (BZE–AChE) possesses an additional recognition element besides the inhibitor binding site. After the deposition of BZE–AChE on the sensor surface the binding of an anti-BZE-antibody to the BZE–AChE can be monitored. This makes it possible to determine two analytes – cocaine and organophosphate – in one experiment by measuring antibody binding and decrease in enzymatic activity, respectively. Furthermore it was also shown that other cocaine-binding enzymes, e.g., butyrylcholinesterase, can bind to the modified BZE–AChE.
The competitive immunoassay allowed the detection of cocaine with a dynamic range from
1
0
−
9
to
1
0
−
7
M. The organophosphate chlorpyrifos-oxon could be detected in concentrations from
1
0
−
6
down to
1
0
−
8
M after 20
min of injection time (equals to 500
μ
L sample volume.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2008.03.027</identifier><identifier>PMID: 18468882</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Acetylcholinesterase ; Acetylcholinesterase - chemistry ; Biological and medical sciences ; Biosensing Techniques - methods ; Biotechnology ; Butyrylcholinesterase - chemistry ; Chlorpyrifos - analogs & derivatives ; Chlorpyrifos - analysis ; Cholinesterase Inhibitors - analysis ; Cocaine ; Cocaine - analogs & derivatives ; Cocaine - analysis ; Cocaine - chemistry ; Cocaine - immunology ; Detection ; Fundamental and applied biological sciences. Psychology ; Haptens - immunology ; Immunoassay - methods ; Modified enzyme ; Organophosphate ; Propidium</subject><ispartof>Biosensors & bioelectronics, 2008-09, Vol.24 (1), p.111-117</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-502e9618b65ed3a3683d7a5f97f1d6e0b6281405e2ed9fd5d5acb5441ebc7c23</citedby><cites>FETCH-LOGICAL-c415t-502e9618b65ed3a3683d7a5f97f1d6e0b6281405e2ed9fd5d5acb5441ebc7c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2008.03.027$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20665451$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18468882$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teller, Carsten</creatorcontrib><creatorcontrib>Halámek, Jan</creatorcontrib><creatorcontrib>Žeravík, Jiři</creatorcontrib><creatorcontrib>Stöcklein, Walter F.M.</creatorcontrib><creatorcontrib>Scheller, Frieder W.</creatorcontrib><title>Development of a bifunctional sensor using haptenized acetylcholinesterase and application for the detection of cocaine and organophosphates</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>We developed a dual piezoelectric/amperometric sensor for the detection of two unrelated analytes in one experiment that uses propidium to anchor acetylcholinesterases (AChE) at the surface. This mass-sensitive sensor does not only allow the examination of the interaction between AChE and the modified surface but also the detection of in situ inhibition of the surface-bound AChE.
Here we describe the application of the propidium-based sensor in combination with a modified AChE. For this reason the cocaine derivative benzoylecgonine (BZE) was coupled via a 10
Å long hydrophilic linker – 1,8-diamino-3,4-dioxaoctane – to carboxylic groups of the AChE after EDC/NHS activation. Thus the modified AChE (BZE–AChE) possesses an additional recognition element besides the inhibitor binding site. After the deposition of BZE–AChE on the sensor surface the binding of an anti-BZE-antibody to the BZE–AChE can be monitored. This makes it possible to determine two analytes – cocaine and organophosphate – in one experiment by measuring antibody binding and decrease in enzymatic activity, respectively. Furthermore it was also shown that other cocaine-binding enzymes, e.g., butyrylcholinesterase, can bind to the modified BZE–AChE.
The competitive immunoassay allowed the detection of cocaine with a dynamic range from
1
0
−
9
to
1
0
−
7
M. The organophosphate chlorpyrifos-oxon could be detected in concentrations from
1
0
−
6
down to
1
0
−
8
M after 20
min of injection time (equals to 500
μ
L sample volume.</description><subject>Acetylcholinesterase</subject><subject>Acetylcholinesterase - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biotechnology</subject><subject>Butyrylcholinesterase - chemistry</subject><subject>Chlorpyrifos - analogs & derivatives</subject><subject>Chlorpyrifos - analysis</subject><subject>Cholinesterase Inhibitors - analysis</subject><subject>Cocaine</subject><subject>Cocaine - analogs & derivatives</subject><subject>Cocaine - analysis</subject><subject>Cocaine - chemistry</subject><subject>Cocaine - immunology</subject><subject>Detection</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Haptens - immunology</subject><subject>Immunoassay - methods</subject><subject>Modified enzyme</subject><subject>Organophosphate</subject><subject>Propidium</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u3CAURlHVqJkmfYEuKjbtzg5gg0Hqpkp_pUjZZI8wXMeMPOCCHSl5hj50cWbU7toVi3u-j6t7EHpLSU0JFVf7uvcx14wQWZOmJqx7gXZUdk3Vsoa_RDuiuKi4EM05ep3znhDSUUVeoXMqWyGlZDv06zM8wBTnA4QFxwEb3PthDXbxMZgJZwg5JrxmH-7xaOYFgn8Ch42F5XGyY5x8gLxAMhmwCWUwz5O3ZovjoSSXEbCDBZ4Ltw9stKZknuGY7k2I8xjzPJoF8iU6G8yU4c3pvUB3X7_cXX-vbm6__bj-dFPZlvKl4oSBElT2goNrTCNk4zrDB9UN1AkgvWCStoQDA6cGxx03tudtS6G3nWXNBfpwrJ1T_LmW9fXBZwvTZALENWuhmBJKqf-CjEgmOtIVkB1Bm2LOCQY9J38w6VFTojdXeq83V3pzpUmji6sSendqX_sDuL-Rk5wCvD8BJlszDckE6_MfjhEheMtp4T4eOSg3e_CQdLYeggXnUzm8dtH_a4_fbHO2eQ</recordid><startdate>20080915</startdate><enddate>20080915</enddate><creator>Teller, Carsten</creator><creator>Halámek, Jan</creator><creator>Žeravík, Jiři</creator><creator>Stöcklein, Walter F.M.</creator><creator>Scheller, Frieder W.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20080915</creationdate><title>Development of a bifunctional sensor using haptenized acetylcholinesterase and application for the detection of cocaine and organophosphates</title><author>Teller, Carsten ; Halámek, Jan ; Žeravík, Jiři ; Stöcklein, Walter F.M. ; Scheller, Frieder W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-502e9618b65ed3a3683d7a5f97f1d6e0b6281405e2ed9fd5d5acb5441ebc7c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acetylcholinesterase</topic><topic>Acetylcholinesterase - chemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biotechnology</topic><topic>Butyrylcholinesterase - chemistry</topic><topic>Chlorpyrifos - analogs & derivatives</topic><topic>Chlorpyrifos - analysis</topic><topic>Cholinesterase Inhibitors - analysis</topic><topic>Cocaine</topic><topic>Cocaine - analogs & derivatives</topic><topic>Cocaine - analysis</topic><topic>Cocaine - chemistry</topic><topic>Cocaine - immunology</topic><topic>Detection</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Haptens - immunology</topic><topic>Immunoassay - methods</topic><topic>Modified enzyme</topic><topic>Organophosphate</topic><topic>Propidium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teller, Carsten</creatorcontrib><creatorcontrib>Halámek, Jan</creatorcontrib><creatorcontrib>Žeravík, Jiři</creatorcontrib><creatorcontrib>Stöcklein, Walter F.M.</creatorcontrib><creatorcontrib>Scheller, Frieder W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teller, Carsten</au><au>Halámek, Jan</au><au>Žeravík, Jiři</au><au>Stöcklein, Walter F.M.</au><au>Scheller, Frieder W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a bifunctional sensor using haptenized acetylcholinesterase and application for the detection of cocaine and organophosphates</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2008-09-15</date><risdate>2008</risdate><volume>24</volume><issue>1</issue><spage>111</spage><epage>117</epage><pages>111-117</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>We developed a dual piezoelectric/amperometric sensor for the detection of two unrelated analytes in one experiment that uses propidium to anchor acetylcholinesterases (AChE) at the surface. This mass-sensitive sensor does not only allow the examination of the interaction between AChE and the modified surface but also the detection of in situ inhibition of the surface-bound AChE.
Here we describe the application of the propidium-based sensor in combination with a modified AChE. For this reason the cocaine derivative benzoylecgonine (BZE) was coupled via a 10
Å long hydrophilic linker – 1,8-diamino-3,4-dioxaoctane – to carboxylic groups of the AChE after EDC/NHS activation. Thus the modified AChE (BZE–AChE) possesses an additional recognition element besides the inhibitor binding site. After the deposition of BZE–AChE on the sensor surface the binding of an anti-BZE-antibody to the BZE–AChE can be monitored. This makes it possible to determine two analytes – cocaine and organophosphate – in one experiment by measuring antibody binding and decrease in enzymatic activity, respectively. Furthermore it was also shown that other cocaine-binding enzymes, e.g., butyrylcholinesterase, can bind to the modified BZE–AChE.
The competitive immunoassay allowed the detection of cocaine with a dynamic range from
1
0
−
9
to
1
0
−
7
M. The organophosphate chlorpyrifos-oxon could be detected in concentrations from
1
0
−
6
down to
1
0
−
8
M after 20
min of injection time (equals to 500
μ
L sample volume.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>18468882</pmid><doi>10.1016/j.bios.2008.03.027</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0956-5663 |
| ispartof | Biosensors & bioelectronics, 2008-09, Vol.24 (1), p.111-117 |
| issn | 0956-5663 1873-4235 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69296999 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acetylcholinesterase Acetylcholinesterase - chemistry Biological and medical sciences Biosensing Techniques - methods Biotechnology Butyrylcholinesterase - chemistry Chlorpyrifos - analogs & derivatives Chlorpyrifos - analysis Cholinesterase Inhibitors - analysis Cocaine Cocaine - analogs & derivatives Cocaine - analysis Cocaine - chemistry Cocaine - immunology Detection Fundamental and applied biological sciences. Psychology Haptens - immunology Immunoassay - methods Modified enzyme Organophosphate Propidium |
| title | Development of a bifunctional sensor using haptenized acetylcholinesterase and application for the detection of cocaine and organophosphates |
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