Daxx interacts with HIV-1 integrase and inhibits lentiviral gene expression
The death-associated protein Daxx is a ubiquitously expressed gene in mammals and is widely involved in transcriptional regulation and cellular intrinsic immune response against incoming virus. We found here that knocking down endogenous Daxx with specific siRNA increased HIV-1-derived lentiviral re...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2008-08, Vol.373 (2), p.241-245 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Huang, Lu Xu, Guan-lan Zhang, Jian-qi Tian, Ling Xue, Jing-lun Chen, Jin-zhong Jia, William |
| description | The death-associated protein Daxx is a ubiquitously expressed gene in mammals and is widely involved in transcriptional regulation and cellular intrinsic immune response against incoming virus. We found here that knocking down endogenous Daxx with specific siRNA increased HIV-1-derived lentiviral reporter gene expression in 293T cells. This repressive effect of Daxx is not due to its inhibition on viral gene integration into the cellular genome and is independent of the ubiquitin promoter on the vFUGW lentiviral vector. Instead, this inhibition is dependent on Daxx’s interaction with HIV-1 integrase. A histone deacetylases (HDACs) inhibitor increased reporter gene expression to the level similar to Daxx knockdown in vFUGW infected cells but there was no additive effect in combination of HDACs inhibitor and Daxx-specific siRNA. Our results suggest that Daxx may associate with HIV-1-derived lentiviral DNA via interacting with HIV-1 integrase and recruit HDACs to viral DNA to repress lentiviral gene expression. |
| doi_str_mv | 10.1016/j.bbrc.2008.06.017 |
| format | Article |
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We found here that knocking down endogenous Daxx with specific siRNA increased HIV-1-derived lentiviral reporter gene expression in 293T cells. This repressive effect of Daxx is not due to its inhibition on viral gene integration into the cellular genome and is independent of the ubiquitin promoter on the vFUGW lentiviral vector. Instead, this inhibition is dependent on Daxx’s interaction with HIV-1 integrase. A histone deacetylases (HDACs) inhibitor increased reporter gene expression to the level similar to Daxx knockdown in vFUGW infected cells but there was no additive effect in combination of HDACs inhibitor and Daxx-specific siRNA. Our results suggest that Daxx may associate with HIV-1-derived lentiviral DNA via interacting with HIV-1 integrase and recruit HDACs to viral DNA to repress lentiviral gene expression.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2008.06.017</identifier><identifier>PMID: 18558084</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adaptor Proteins, Signal Transducing - genetics ; Adaptor Proteins, Signal Transducing - metabolism ; Cell Line ; Daxx ; DNA, Viral - metabolism ; Gene expression ; Gene Expression Regulation, Viral ; Genes, Reporter ; Histone Deacetylase Inhibitors ; Histone deacetylases (HDACs) ; Histone Deacetylases - metabolism ; HIV Integrase - metabolism ; HIV-1 - genetics ; HIV-1 integrase ; Human immunodeficiency virus 1 ; Humans ; Lentivirus ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Promoter Regions, Genetic ; RNA, Small Interfering - genetics ; Two-Hybrid System Techniques ; Ubiquitin - genetics</subject><ispartof>Biochemical and biophysical research communications, 2008-08, Vol.373 (2), p.241-245</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-fa85d8fc2518811a39bd569985886f0f94903750edf4265855509ef41e4af1d53</citedby><cites>FETCH-LOGICAL-c385t-fa85d8fc2518811a39bd569985886f0f94903750edf4265855509ef41e4af1d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2008.06.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18558084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Lu</creatorcontrib><creatorcontrib>Xu, Guan-lan</creatorcontrib><creatorcontrib>Zhang, Jian-qi</creatorcontrib><creatorcontrib>Tian, Ling</creatorcontrib><creatorcontrib>Xue, Jing-lun</creatorcontrib><creatorcontrib>Chen, Jin-zhong</creatorcontrib><creatorcontrib>Jia, William</creatorcontrib><title>Daxx interacts with HIV-1 integrase and inhibits lentiviral gene expression</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The death-associated protein Daxx is a ubiquitously expressed gene in mammals and is widely involved in transcriptional regulation and cellular intrinsic immune response against incoming virus. We found here that knocking down endogenous Daxx with specific siRNA increased HIV-1-derived lentiviral reporter gene expression in 293T cells. This repressive effect of Daxx is not due to its inhibition on viral gene integration into the cellular genome and is independent of the ubiquitin promoter on the vFUGW lentiviral vector. Instead, this inhibition is dependent on Daxx’s interaction with HIV-1 integrase. A histone deacetylases (HDACs) inhibitor increased reporter gene expression to the level similar to Daxx knockdown in vFUGW infected cells but there was no additive effect in combination of HDACs inhibitor and Daxx-specific siRNA. Our results suggest that Daxx may associate with HIV-1-derived lentiviral DNA via interacting with HIV-1 integrase and recruit HDACs to viral DNA to repress lentiviral gene expression.</description><subject>Adaptor Proteins, Signal Transducing - genetics</subject><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>Cell Line</subject><subject>Daxx</subject><subject>DNA, Viral - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Viral</subject><subject>Genes, Reporter</subject><subject>Histone Deacetylase Inhibitors</subject><subject>Histone deacetylases (HDACs)</subject><subject>Histone Deacetylases - metabolism</subject><subject>HIV Integrase - metabolism</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 integrase</subject><subject>Human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Lentivirus</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>RNA, Small Interfering - genetics</subject><subject>Two-Hybrid System Techniques</subject><subject>Ubiquitin - genetics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EgvL4ARYoK3YJM2lsbIkN4lUEEhtA7CzHGRdXaVLstJS_J6WV2MFqNKNzr0aHsWOEDAHF2SQry2CzHEBmIDLA8y02QFCQ5gjFNhsAgEhzhW97bD_GCQBiIdQu20PJuQRZDNjDtVkuE990FIztYvLpu_dkdP-a4s9xHEykxDRVv7370vdETU3nFz6YOhlTQwktZ4Fi9G1zyHacqSMdbeYBe7m9eb4apY9Pd_dXl4-pHUrepc5IXklnc45SIpqhKisulJJcSuHAqULB8JwDVa7IBe9f5aDIFUiFcVjx4QE7XffOQvsxp9jpqY-W6to01M6jFipXHFH8C-aguJAF9mC-Bm1oYwzk9Cz4qQlfGkGvXOuJXrnWK9cahO5d96GTTfu8nFL1G9nI7YGLNUC9jIWnoKP11FiqfCDb6ar1f_V_A9OAjso</recordid><startdate>20080822</startdate><enddate>20080822</enddate><creator>Huang, Lu</creator><creator>Xu, Guan-lan</creator><creator>Zhang, Jian-qi</creator><creator>Tian, Ling</creator><creator>Xue, Jing-lun</creator><creator>Chen, Jin-zhong</creator><creator>Jia, William</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080822</creationdate><title>Daxx interacts with HIV-1 integrase and inhibits lentiviral gene expression</title><author>Huang, Lu ; Xu, Guan-lan ; Zhang, Jian-qi ; Tian, Ling ; Xue, Jing-lun ; Chen, Jin-zhong ; Jia, William</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-fa85d8fc2518811a39bd569985886f0f94903750edf4265855509ef41e4af1d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adaptor Proteins, Signal Transducing - genetics</topic><topic>Adaptor Proteins, Signal Transducing - metabolism</topic><topic>Cell Line</topic><topic>Daxx</topic><topic>DNA, Viral - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Viral</topic><topic>Genes, Reporter</topic><topic>Histone Deacetylase Inhibitors</topic><topic>Histone deacetylases (HDACs)</topic><topic>Histone Deacetylases - metabolism</topic><topic>HIV Integrase - metabolism</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 integrase</topic><topic>Human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Lentivirus</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>RNA, Small Interfering - genetics</topic><topic>Two-Hybrid System Techniques</topic><topic>Ubiquitin - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Lu</creatorcontrib><creatorcontrib>Xu, Guan-lan</creatorcontrib><creatorcontrib>Zhang, Jian-qi</creatorcontrib><creatorcontrib>Tian, Ling</creatorcontrib><creatorcontrib>Xue, Jing-lun</creatorcontrib><creatorcontrib>Chen, Jin-zhong</creatorcontrib><creatorcontrib>Jia, William</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Lu</au><au>Xu, Guan-lan</au><au>Zhang, Jian-qi</au><au>Tian, Ling</au><au>Xue, Jing-lun</au><au>Chen, Jin-zhong</au><au>Jia, William</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Daxx interacts with HIV-1 integrase and inhibits lentiviral gene expression</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2008-08-22</date><risdate>2008</risdate><volume>373</volume><issue>2</issue><spage>241</spage><epage>245</epage><pages>241-245</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The death-associated protein Daxx is a ubiquitously expressed gene in mammals and is widely involved in transcriptional regulation and cellular intrinsic immune response against incoming virus. We found here that knocking down endogenous Daxx with specific siRNA increased HIV-1-derived lentiviral reporter gene expression in 293T cells. This repressive effect of Daxx is not due to its inhibition on viral gene integration into the cellular genome and is independent of the ubiquitin promoter on the vFUGW lentiviral vector. Instead, this inhibition is dependent on Daxx’s interaction with HIV-1 integrase. A histone deacetylases (HDACs) inhibitor increased reporter gene expression to the level similar to Daxx knockdown in vFUGW infected cells but there was no additive effect in combination of HDACs inhibitor and Daxx-specific siRNA. Our results suggest that Daxx may associate with HIV-1-derived lentiviral DNA via interacting with HIV-1 integrase and recruit HDACs to viral DNA to repress lentiviral gene expression.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18558084</pmid><doi>10.1016/j.bbrc.2008.06.017</doi><tpages>5</tpages></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - metabolism Cell Line Daxx DNA, Viral - metabolism Gene expression Gene Expression Regulation, Viral Genes, Reporter Histone Deacetylase Inhibitors Histone deacetylases (HDACs) Histone Deacetylases - metabolism HIV Integrase - metabolism HIV-1 - genetics HIV-1 integrase Human immunodeficiency virus 1 Humans Lentivirus Nuclear Proteins - genetics Nuclear Proteins - metabolism Promoter Regions, Genetic RNA, Small Interfering - genetics Two-Hybrid System Techniques Ubiquitin - genetics |
| title | Daxx interacts with HIV-1 integrase and inhibits lentiviral gene expression |
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