Kinetics of synaptotagmin responses to Ca2+ and assembly with the core SNARE complex onto membranes
The synaptic vesicle protein synaptotagmin I binds Ca2+ and is required for efficient neurotransmitter release. Here, we measure the response time of the C2 domains of synaptotagmin to determine whether synaptotagmin is fast enough to function as a Ca2+ sensor for rapid exocytosis. We report that sy...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 1999-10, Vol.24 (2), p.363-376 |
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| container_end_page | 376 |
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| container_title | Neuron (Cambridge, Mass.) |
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| creator | Davis, A F Bai, J Fasshauer, D Wolowick, M J Lewis, J L Chapman, E R |
| description | The synaptic vesicle protein synaptotagmin I binds Ca2+ and is required for efficient neurotransmitter release. Here, we measure the response time of the C2 domains of synaptotagmin to determine whether synaptotagmin is fast enough to function as a Ca2+ sensor for rapid exocytosis. We report that synaptotagmin is "tuned" to sense Ca2+ concentrations that trigger neuronal exocytosis. The speed of response is unique to synaptotagmin I and readily satisfies the kinetic constraints of synaptic vesicle membrane fusion. We further demonstrate that Ca2+ triggers penetration of synaptotagmin into membranes and simultaneously drives assembly of synaptotagmin onto the base of the ternary SNARE (soluble N-ethylmaleimide-sensitive fusion protein [NSF] attachment receptor) complex, near the transmembrane anchor of syntaxin. These data support a molecular model in which synaptotagmin triggers exocytosis through its interactions with membranes and the SNARE complex. |
| doi_str_mv | 10.1016/S0896-6273(00)80850-8 |
| format | Article |
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| ispartof | Neuron (Cambridge, Mass.), 1999-10, Vol.24 (2), p.363-376 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Cell Press Free Archives; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Calcium - physiology Calcium-Binding Proteins Kinetics Membrane Fusion - physiology Membrane Glycoproteins - chemistry Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Membrane Proteins - metabolism Membranes - metabolism Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Rats Repetitive Sequences, Nucleic Acid Sequence Homology SNARE Proteins Synaptic Vesicles - metabolism Synaptotagmin I Synaptotagmins Vesicular Transport Proteins |
| title | Kinetics of synaptotagmin responses to Ca2+ and assembly with the core SNARE complex onto membranes |
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