The effect of cell wall components on glycine-enhanced sterol side chain degradation to androstene derivatives by mycobacteria
beta-Sitosterol side chain degradation by Mycobacterium sp. NRRL MB 3683 results in the formation of androstene derivatives and is increased in the presence of glycine. As the sterol transformation is carried out inside the cell, higher product accumulation could indicate faster diffusion of highly...
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| Veröffentlicht in: | Applied microbiology and biotechnology 1999-10, Vol.52 (4), p.563-571 |
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| creator | SEDLACZEK, L LISOWSKA, K KORYCKA, M RUMIJOWSKA, A ZIOŁKOWSKI, A DŁUGONSKI, J |
| description | beta-Sitosterol side chain degradation by Mycobacterium sp. NRRL MB 3683 results in the formation of androstene derivatives and is increased in the presence of glycine. As the sterol transformation is carried out inside the cell, higher product accumulation could indicate faster diffusion of highly hydrophobic substrate through the cell wall permeability barrier. Cell wall preparations were obtained to analyse the effect of glycine on peptidoglycan components. Peptidoglycan is known to be the target for glycine action. In glycine-treated preparations, the molar ratio of diaminopimelic acid:muramic acid, the marker compounds of tetrapeptides and glycan strands respectively, was about 60% lower than in the control. This indicates a possible reduction in cross-linking between peptide units and the destruction of peptidoglycan. Unexpectedly, glycine also caused changes in the relative proportion of mycolic acids to other lipids occurring in the strain used for this study. The enhancement of beta-sitosterol side chain degradation is likely to result from disturbing the integrity of the cell wall components responsible for the permeability barrier in mycobacteria. |
| doi_str_mv | 10.1007/s002530051561 |
| format | Article |
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NRRL MB 3683 results in the formation of androstene derivatives and is increased in the presence of glycine. As the sterol transformation is carried out inside the cell, higher product accumulation could indicate faster diffusion of highly hydrophobic substrate through the cell wall permeability barrier. Cell wall preparations were obtained to analyse the effect of glycine on peptidoglycan components. Peptidoglycan is known to be the target for glycine action. In glycine-treated preparations, the molar ratio of diaminopimelic acid:muramic acid, the marker compounds of tetrapeptides and glycan strands respectively, was about 60% lower than in the control. This indicates a possible reduction in cross-linking between peptide units and the destruction of peptidoglycan. Unexpectedly, glycine also caused changes in the relative proportion of mycolic acids to other lipids occurring in the strain used for this study. The enhancement of beta-sitosterol side chain degradation is likely to result from disturbing the integrity of the cell wall components responsible for the permeability barrier in mycobacteria.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s002530051561</identifier><identifier>PMID: 10570804</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>androstene ; androstene derivatives ; Androstenes - metabolism ; b-Sitosterol ; Biochemistry ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biomass ; Biotechnology ; Biotransformation - drug effects ; Cell Wall - chemistry ; Cell Wall - metabolism ; Densitometry ; diaminopimelic acid ; Fundamental and applied biological sciences. Psychology ; glycine ; Glycine - pharmacology ; Lipids ; Mass Spectrometry ; Membrane Lipids - metabolism ; Mission oriented research ; muramic acid ; Mycobacterium ; Mycobacterium - drug effects ; Mycobacterium - growth & development ; Mycobacterium - metabolism ; mycolic acids ; Mycolic Acids - metabolism ; Peptidoglycan - drug effects ; Peptidoglycan - metabolism ; peptidoglycans ; Permeability ; Physiology and metabolism ; sitosterol ; Sitosterols - metabolism ; Sterols</subject><ispartof>Applied microbiology and biotechnology, 1999-10, Vol.52 (4), p.563-571</ispartof><rights>1999 INIST-CNRS</rights><rights>Springer-Verlag Berlin Heidelberg 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-6492de04db96a5858b58f2ecad3eed5361f7800addab90159fa626d8f6d37f023</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1980782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10570804$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SEDLACZEK, L</creatorcontrib><creatorcontrib>LISOWSKA, K</creatorcontrib><creatorcontrib>KORYCKA, M</creatorcontrib><creatorcontrib>RUMIJOWSKA, A</creatorcontrib><creatorcontrib>ZIOŁKOWSKI, A</creatorcontrib><creatorcontrib>DŁUGONSKI, J</creatorcontrib><title>The effect of cell wall components on glycine-enhanced sterol side chain degradation to androstene derivatives by mycobacteria</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>beta-Sitosterol side chain degradation by Mycobacterium sp. NRRL MB 3683 results in the formation of androstene derivatives and is increased in the presence of glycine. As the sterol transformation is carried out inside the cell, higher product accumulation could indicate faster diffusion of highly hydrophobic substrate through the cell wall permeability barrier. Cell wall preparations were obtained to analyse the effect of glycine on peptidoglycan components. Peptidoglycan is known to be the target for glycine action. In glycine-treated preparations, the molar ratio of diaminopimelic acid:muramic acid, the marker compounds of tetrapeptides and glycan strands respectively, was about 60% lower than in the control. This indicates a possible reduction in cross-linking between peptide units and the destruction of peptidoglycan. Unexpectedly, glycine also caused changes in the relative proportion of mycolic acids to other lipids occurring in the strain used for this study. The enhancement of beta-sitosterol side chain degradation is likely to result from disturbing the integrity of the cell wall components responsible for the permeability barrier in mycobacteria.</description><subject>androstene</subject><subject>androstene derivatives</subject><subject>Androstenes - metabolism</subject><subject>b-Sitosterol</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biomass</subject><subject>Biotechnology</subject><subject>Biotransformation - drug effects</subject><subject>Cell Wall - chemistry</subject><subject>Cell Wall - metabolism</subject><subject>Densitometry</subject><subject>diaminopimelic acid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glycine</subject><subject>Glycine - pharmacology</subject><subject>Lipids</subject><subject>Mass Spectrometry</subject><subject>Membrane Lipids - metabolism</subject><subject>Mission oriented research</subject><subject>muramic acid</subject><subject>Mycobacterium</subject><subject>Mycobacterium - drug effects</subject><subject>Mycobacterium - growth & development</subject><subject>Mycobacterium - metabolism</subject><subject>mycolic acids</subject><subject>Mycolic Acids - metabolism</subject><subject>Peptidoglycan - drug effects</subject><subject>Peptidoglycan - metabolism</subject><subject>peptidoglycans</subject><subject>Permeability</subject><subject>Physiology and metabolism</subject><subject>sitosterol</subject><subject>Sitosterols - metabolism</subject><subject>Sterols</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0j1rHDEQBmAREuKLnTJtECG4W3skrT62DCZxAgY3Tr1opZFvza50kfYcrslvj447yEdzjVTMwyC9M4S8Y3DFAPR1AeBSAEgmFXtBVqwVvAHF2pdkBUzLRsvOnJE3pTwBMG6Uek3OGEgNBtoV-fWwRoohoFtoCtThNNGfth4uzZsUMS6Fpkgfp50bIzYY1zY69LQsmNNEy-iRurUdI_X4mK23y1j5kqiNPqeqItZKHp9r4RkLHXZ03rk0WFcbjPaCvAp2Kvj2eJ-T718-P9x8be7ub7_dfLprXCvM0qi24x6h9UOnrDTSDNIEjs56geilUCxoA2C9t0MHTHbBKq68CcoLHYCLc3J56LvJ6ccWy9LPY9l_1kZM29KrjnetYOok5DVEUFKehMy0XHRMnIZaGC04q_DDf_ApbXOssfTGtEqCVvv3NQfkarolY-g3eZxt3vUM-v1C9P8sRPXvj023w4z-L33YgAo-HoEtzk4h1_mO5Y_rDGjDxW_FL72b</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>SEDLACZEK, L</creator><creator>LISOWSKA, K</creator><creator>KORYCKA, M</creator><creator>RUMIJOWSKA, A</creator><creator>ZIOŁKOWSKI, A</creator><creator>DŁUGONSKI, J</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>The effect of cell wall components on glycine-enhanced sterol side chain degradation to androstene derivatives by mycobacteria</title><author>SEDLACZEK, L ; LISOWSKA, K ; KORYCKA, M ; RUMIJOWSKA, A ; ZIOŁKOWSKI, A ; DŁUGONSKI, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-6492de04db96a5858b58f2ecad3eed5361f7800addab90159fa626d8f6d37f023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>androstene</topic><topic>androstene derivatives</topic><topic>Androstenes - metabolism</topic><topic>b-Sitosterol</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biomass</topic><topic>Biotechnology</topic><topic>Biotransformation - drug effects</topic><topic>Cell Wall - chemistry</topic><topic>Cell Wall - metabolism</topic><topic>Densitometry</topic><topic>diaminopimelic acid</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SEDLACZEK, L</au><au>LISOWSKA, K</au><au>KORYCKA, M</au><au>RUMIJOWSKA, A</au><au>ZIOŁKOWSKI, A</au><au>DŁUGONSKI, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of cell wall components on glycine-enhanced sterol side chain degradation to androstene derivatives by mycobacteria</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>52</volume><issue>4</issue><spage>563</spage><epage>571</epage><pages>563-571</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>beta-Sitosterol side chain degradation by Mycobacterium sp. NRRL MB 3683 results in the formation of androstene derivatives and is increased in the presence of glycine. As the sterol transformation is carried out inside the cell, higher product accumulation could indicate faster diffusion of highly hydrophobic substrate through the cell wall permeability barrier. Cell wall preparations were obtained to analyse the effect of glycine on peptidoglycan components. Peptidoglycan is known to be the target for glycine action. In glycine-treated preparations, the molar ratio of diaminopimelic acid:muramic acid, the marker compounds of tetrapeptides and glycan strands respectively, was about 60% lower than in the control. This indicates a possible reduction in cross-linking between peptide units and the destruction of peptidoglycan. Unexpectedly, glycine also caused changes in the relative proportion of mycolic acids to other lipids occurring in the strain used for this study. The enhancement of beta-sitosterol side chain degradation is likely to result from disturbing the integrity of the cell wall components responsible for the permeability barrier in mycobacteria.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>10570804</pmid><doi>10.1007/s002530051561</doi><tpages>9</tpages></addata></record> |
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| subjects | androstene androstene derivatives Androstenes - metabolism b-Sitosterol Biochemistry Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biomass Biotechnology Biotransformation - drug effects Cell Wall - chemistry Cell Wall - metabolism Densitometry diaminopimelic acid Fundamental and applied biological sciences. Psychology glycine Glycine - pharmacology Lipids Mass Spectrometry Membrane Lipids - metabolism Mission oriented research muramic acid Mycobacterium Mycobacterium - drug effects Mycobacterium - growth & development Mycobacterium - metabolism mycolic acids Mycolic Acids - metabolism Peptidoglycan - drug effects Peptidoglycan - metabolism peptidoglycans Permeability Physiology and metabolism sitosterol Sitosterols - metabolism Sterols |
| title | The effect of cell wall components on glycine-enhanced sterol side chain degradation to androstene derivatives by mycobacteria |
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