Rat sensory neurons contain cytochrome b558 large subunit immunoreactivity
CYTOCHROME b558 is part of the NADPH oxidase complex of phagocytes, but it has also been proposed to function as a cellular oxygen sensor, e.g. in the carotid body. Thus, we investigated whether cytochrome b558 is present in rat primary afferent neurons. Immunohisto-chemistry and Western blotting us...
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| Veröffentlicht in: | Neuroreport 1999-08, Vol.10 (12), p.2615-2617 |
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| creator | Dvorakova, Magdalena Höhler, Brigitte Richter, Elke Burritt, James B Kummer, Wolfgang |
| description | CYTOCHROME b558 is part of the NADPH oxidase complex of phagocytes, but it has also been proposed to function as a cellular oxygen sensor, e.g. in the carotid body. Thus, we investigated whether cytochrome b558 is present in rat primary afferent neurons. Immunohisto-chemistry and Western blotting using the monoclonal antibody 54.1 directed towards the large subunit of cytochrome b558, gp91, revealed a ubiquituous occurrence of cytochrome b558-immunoreactivity in neurons of the petrosal ganglion that innervates the carotid body, and also in dorsal root ganglia. This ubiquituous occurrence in sensory neurons of various locations and functional modalities points to a general role of cytochrome b558 in primary afferent neurons rather than involvement in a specialized function such as arterial chemoreception. |
| doi_str_mv | 10.1097/00001756-199908200-00032 |
| format | Article |
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Psychology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>NADPH Oxidases</topic><topic>Neurons, Afferent - physiology</topic><topic>Peptide Fragments - analysis</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dvorakova, Magdalena</creatorcontrib><creatorcontrib>Höhler, Brigitte</creatorcontrib><creatorcontrib>Richter, Elke</creatorcontrib><creatorcontrib>Burritt, James B</creatorcontrib><creatorcontrib>Kummer, Wolfgang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dvorakova, Magdalena</au><au>Höhler, Brigitte</au><au>Richter, Elke</au><au>Burritt, James B</au><au>Kummer, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rat sensory neurons contain cytochrome b558 large subunit immunoreactivity</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>1999-08-20</date><risdate>1999</risdate><volume>10</volume><issue>12</issue><spage>2615</spage><epage>2617</epage><pages>2615-2617</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>CYTOCHROME b558 is part of the NADPH oxidase complex of phagocytes, but it has also been proposed to function as a cellular oxygen sensor, e.g. in the carotid body. Thus, we investigated whether cytochrome b558 is present in rat primary afferent neurons. Immunohisto-chemistry and Western blotting using the monoclonal antibody 54.1 directed towards the large subunit of cytochrome b558, gp91, revealed a ubiquituous occurrence of cytochrome b558-immunoreactivity in neurons of the petrosal ganglion that innervates the carotid body, and also in dorsal root ganglia. This ubiquituous occurrence in sensory neurons of various locations and functional modalities points to a general role of cytochrome b558 in primary afferent neurons rather than involvement in a specialized function such as arterial chemoreception.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>10574379</pmid><doi>10.1097/00001756-199908200-00032</doi><tpages>3</tpages></addata></record> |
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| source | Journals@Ovid Ovid Autoload; MEDLINE |
| subjects | Animals Antibodies, Monoclonal Biological and medical sciences Blotting, Western Cytochrome b Group - chemistry Female Fundamental and applied biological sciences. Psychology Immunohistochemistry Male NADPH Oxidases Neurons, Afferent - physiology Peptide Fragments - analysis Rats Rats, Wistar Somesthesis and somesthetic pathways (proprioception, exteroception, nociception) interoception electrolocation. Sensory receptors Vertebrates: nervous system and sense organs |
| title | Rat sensory neurons contain cytochrome b558 large subunit immunoreactivity |
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