Molecular analysis of the pheromone and pheromone receptor genes of Ustilago hordei
Cell–cell signaling is an integral part of the sexual and disease cycles of the smut fungi, which must mate to be pathogenic. This study reports the cloning and characterization of the pheromone genes Uhmfa1 and Uhmfa2 from MAT-1 and MAT-2 mating types of U. hordei, respectively, and the pheromone r...
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| Veröffentlicht in: | Gene 1999-11, Vol.240 (1), p.89-97 |
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| creator | Anderson, Cynthia M. Willits, Deborah A. Kosted, Paula J. Ford, Eugene J. Martinez-Espinoza, Alfredo D. Sherwood, John E. |
| description | Cell–cell signaling is an integral part of the sexual and disease cycles of the smut fungi, which must mate to be pathogenic. This study reports the cloning and characterization of the pheromone genes
Uhmfa1 and
Uhmfa2 from MAT-1 and MAT-2 mating types of
U. hordei, respectively, and the pheromone receptor gene
Uhpra2 from MAT-2 cells. Similar to other fungal pheromone genes,
Uhmfa1 and
Uhmfa2 encode precursor peptides.
Uhpra2 encodes a protein with sequence similarity to the 7-transmembrane class of G-protein coupled receptors. Deletion of
Uhmfa1 and
Uhpra1, and their subsequent replacement, confirmed the role of these genes in initiation of the sexual cycle.
Uhmfa1 and
Uhmfa2 were differentially expressed in various cell types and when opposite mating-type cells were grown together. The predicted mature pheromones of each mating type were synthesized, and each specifically induced conjugation tube formation in cells of the opposite mating type. |
| doi_str_mv | 10.1016/S0378-1119(99)00428-X |
| format | Article |
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Uhmfa1 and
Uhmfa2 from MAT-1 and MAT-2 mating types of
U. hordei, respectively, and the pheromone receptor gene
Uhpra2 from MAT-2 cells. Similar to other fungal pheromone genes,
Uhmfa1 and
Uhmfa2 encode precursor peptides.
Uhpra2 encodes a protein with sequence similarity to the 7-transmembrane class of G-protein coupled receptors. Deletion of
Uhmfa1 and
Uhpra1, and their subsequent replacement, confirmed the role of these genes in initiation of the sexual cycle.
Uhmfa1 and
Uhmfa2 were differentially expressed in various cell types and when opposite mating-type cells were grown together. The predicted mature pheromones of each mating type were synthesized, and each specifically induced conjugation tube formation in cells of the opposite mating type.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(99)00428-X</identifier><identifier>PMID: 10564815</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Barley-covered smut ; Base Sequence ; Chemoreceptor Cells ; cloning ; Cloning, Molecular ; deletions ; DNA, Fungal - chemistry ; DNA, Fungal - genetics ; Fungal Proteins ; genbank/af043940 ; genbank/af184069 ; genbank/af184070 ; Gene Deletion ; Gene disruption ; Gene Expression Regulation, Fungal ; genes ; Genes, Fungal - genetics ; Genes, Mating Type, Fungal ; introns ; loci ; mat-1 locus ; mating behavior ; Molecular cloning ; Molecular Sequence Data ; mutants ; nucleotide sequences ; open reading frames ; pheromone receptors ; pheromone response elements ; pheromones ; Pheromones - chemical synthesis ; Pheromones - genetics ; Pheromones - pharmacology ; promoter regions ; Promoter Regions, Genetic ; Protein Precursors - genetics ; receptors ; Reproduction - drug effects ; Sequence Analysis, DNA ; sexual reproduction ; TATA Box ; Uhmfa1 gene ; Uhmfa2 gene ; Uhpra2 gene ; Ustilago - cytology ; Ustilago - drug effects ; Ustilago - genetics ; Ustilago hordei ; Ustilago segetum</subject><ispartof>Gene, 1999-11, Vol.240 (1), p.89-97</ispartof><rights>1999 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-de4cc08251c4e99ca86433630156c87025e8c0064c78bdbd7a4be2b0785abd8c3</citedby><cites>FETCH-LOGICAL-c416t-de4cc08251c4e99ca86433630156c87025e8c0064c78bdbd7a4be2b0785abd8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1119(99)00428-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10564815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anderson, Cynthia M.</creatorcontrib><creatorcontrib>Willits, Deborah A.</creatorcontrib><creatorcontrib>Kosted, Paula J.</creatorcontrib><creatorcontrib>Ford, Eugene J.</creatorcontrib><creatorcontrib>Martinez-Espinoza, Alfredo D.</creatorcontrib><creatorcontrib>Sherwood, John E.</creatorcontrib><title>Molecular analysis of the pheromone and pheromone receptor genes of Ustilago hordei</title><title>Gene</title><addtitle>Gene</addtitle><description>Cell–cell signaling is an integral part of the sexual and disease cycles of the smut fungi, which must mate to be pathogenic. This study reports the cloning and characterization of the pheromone genes
Uhmfa1 and
Uhmfa2 from MAT-1 and MAT-2 mating types of
U. hordei, respectively, and the pheromone receptor gene
Uhpra2 from MAT-2 cells. Similar to other fungal pheromone genes,
Uhmfa1 and
Uhmfa2 encode precursor peptides.
Uhpra2 encodes a protein with sequence similarity to the 7-transmembrane class of G-protein coupled receptors. Deletion of
Uhmfa1 and
Uhpra1, and their subsequent replacement, confirmed the role of these genes in initiation of the sexual cycle.
Uhmfa1 and
Uhmfa2 were differentially expressed in various cell types and when opposite mating-type cells were grown together. The predicted mature pheromones of each mating type were synthesized, and each specifically induced conjugation tube formation in cells of the opposite mating type.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Barley-covered smut</subject><subject>Base Sequence</subject><subject>Chemoreceptor Cells</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>deletions</subject><subject>DNA, Fungal - chemistry</subject><subject>DNA, Fungal - genetics</subject><subject>Fungal Proteins</subject><subject>genbank/af043940</subject><subject>genbank/af184069</subject><subject>genbank/af184070</subject><subject>Gene Deletion</subject><subject>Gene disruption</subject><subject>Gene Expression Regulation, Fungal</subject><subject>genes</subject><subject>Genes, Fungal - genetics</subject><subject>Genes, Mating Type, Fungal</subject><subject>introns</subject><subject>loci</subject><subject>mat-1 locus</subject><subject>mating behavior</subject><subject>Molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>mutants</subject><subject>nucleotide sequences</subject><subject>open reading frames</subject><subject>pheromone receptors</subject><subject>pheromone response elements</subject><subject>pheromones</subject><subject>Pheromones - chemical synthesis</subject><subject>Pheromones - genetics</subject><subject>Pheromones - pharmacology</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Precursors - genetics</subject><subject>receptors</subject><subject>Reproduction - drug effects</subject><subject>Sequence Analysis, DNA</subject><subject>sexual reproduction</subject><subject>TATA Box</subject><subject>Uhmfa1 gene</subject><subject>Uhmfa2 gene</subject><subject>Uhpra2 gene</subject><subject>Ustilago - cytology</subject><subject>Ustilago - drug effects</subject><subject>Ustilago - genetics</subject><subject>Ustilago hordei</subject><subject>Ustilago segetum</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3DAQgEVpaTZpfkJbn0JycDojS7Z0KiXkUUjJYbOQm5Cl2V0Vr7WVvIH8-zrrEHLLXIZhvnnwMfYV4RwB6x9zqBpVIqI-1foMQHBVPnxgM1SNLgEq9ZHNXpEDdpjzXxhDSv6ZHSDIWiiUMzb_Eztyu86mwva2e8ohF3FZDGsqtmtKcRN7Gjv-TZXI0XaIqVhRT3t6kYfQ2VUs1jF5Cl_Yp6XtMh2_5CO2uLq8v7gpb--uf1_8ui2dwHooPQnnQHGJTpDWzqpaVFVdAcraqQa4JOUAauEa1frWN1a0xFtolLStV646YifT3m2K_3aUB7MJ2VHX2Z7iLptacyUr1O-C2AgQjVYjKCfQpZhzoqXZprCx6ckgmGftZq_dPDs1Wpu9dvMwzn17ObBrN-TfTE2eR-D7BCxtNHaVQjaLOQesgGvUkuNI_JwIGo09Bkomu0C9Ix9G4YPxMbzzxH_dY5ue</recordid><startdate>19991115</startdate><enddate>19991115</enddate><creator>Anderson, Cynthia M.</creator><creator>Willits, Deborah A.</creator><creator>Kosted, Paula J.</creator><creator>Ford, Eugene J.</creator><creator>Martinez-Espinoza, Alfredo D.</creator><creator>Sherwood, John E.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19991115</creationdate><title>Molecular analysis of the pheromone and pheromone receptor genes of Ustilago hordei</title><author>Anderson, Cynthia M. ; Willits, Deborah A. ; Kosted, Paula J. ; Ford, Eugene J. ; Martinez-Espinoza, Alfredo D. ; Sherwood, John E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-de4cc08251c4e99ca86433630156c87025e8c0064c78bdbd7a4be2b0785abd8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Barley-covered smut</topic><topic>Base Sequence</topic><topic>Chemoreceptor Cells</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>deletions</topic><topic>DNA, Fungal - chemistry</topic><topic>DNA, Fungal - genetics</topic><topic>Fungal Proteins</topic><topic>genbank/af043940</topic><topic>genbank/af184069</topic><topic>genbank/af184070</topic><topic>Gene Deletion</topic><topic>Gene disruption</topic><topic>Gene Expression Regulation, Fungal</topic><topic>genes</topic><topic>Genes, Fungal - genetics</topic><topic>Genes, Mating Type, Fungal</topic><topic>introns</topic><topic>loci</topic><topic>mat-1 locus</topic><topic>mating behavior</topic><topic>Molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>mutants</topic><topic>nucleotide sequences</topic><topic>open reading frames</topic><topic>pheromone receptors</topic><topic>pheromone response elements</topic><topic>pheromones</topic><topic>Pheromones - chemical synthesis</topic><topic>Pheromones - genetics</topic><topic>Pheromones - pharmacology</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Precursors - genetics</topic><topic>receptors</topic><topic>Reproduction - drug effects</topic><topic>Sequence Analysis, DNA</topic><topic>sexual reproduction</topic><topic>TATA Box</topic><topic>Uhmfa1 gene</topic><topic>Uhmfa2 gene</topic><topic>Uhpra2 gene</topic><topic>Ustilago - cytology</topic><topic>Ustilago - drug effects</topic><topic>Ustilago - genetics</topic><topic>Ustilago hordei</topic><topic>Ustilago segetum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anderson, Cynthia M.</creatorcontrib><creatorcontrib>Willits, Deborah A.</creatorcontrib><creatorcontrib>Kosted, Paula J.</creatorcontrib><creatorcontrib>Ford, Eugene J.</creatorcontrib><creatorcontrib>Martinez-Espinoza, Alfredo D.</creatorcontrib><creatorcontrib>Sherwood, John E.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anderson, Cynthia M.</au><au>Willits, Deborah A.</au><au>Kosted, Paula J.</au><au>Ford, Eugene J.</au><au>Martinez-Espinoza, Alfredo D.</au><au>Sherwood, John E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of the pheromone and pheromone receptor genes of Ustilago hordei</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1999-11-15</date><risdate>1999</risdate><volume>240</volume><issue>1</issue><spage>89</spage><epage>97</epage><pages>89-97</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Cell–cell signaling is an integral part of the sexual and disease cycles of the smut fungi, which must mate to be pathogenic. This study reports the cloning and characterization of the pheromone genes
Uhmfa1 and
Uhmfa2 from MAT-1 and MAT-2 mating types of
U. hordei, respectively, and the pheromone receptor gene
Uhpra2 from MAT-2 cells. Similar to other fungal pheromone genes,
Uhmfa1 and
Uhmfa2 encode precursor peptides.
Uhpra2 encodes a protein with sequence similarity to the 7-transmembrane class of G-protein coupled receptors. Deletion of
Uhmfa1 and
Uhpra1, and their subsequent replacement, confirmed the role of these genes in initiation of the sexual cycle.
Uhmfa1 and
Uhmfa2 were differentially expressed in various cell types and when opposite mating-type cells were grown together. The predicted mature pheromones of each mating type were synthesized, and each specifically induced conjugation tube formation in cells of the opposite mating type.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10564815</pmid><doi>10.1016/S0378-1119(99)00428-X</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1999-11, Vol.240 (1), p.89-97 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence amino acid sequences Barley-covered smut Base Sequence Chemoreceptor Cells cloning Cloning, Molecular deletions DNA, Fungal - chemistry DNA, Fungal - genetics Fungal Proteins genbank/af043940 genbank/af184069 genbank/af184070 Gene Deletion Gene disruption Gene Expression Regulation, Fungal genes Genes, Fungal - genetics Genes, Mating Type, Fungal introns loci mat-1 locus mating behavior Molecular cloning Molecular Sequence Data mutants nucleotide sequences open reading frames pheromone receptors pheromone response elements pheromones Pheromones - chemical synthesis Pheromones - genetics Pheromones - pharmacology promoter regions Promoter Regions, Genetic Protein Precursors - genetics receptors Reproduction - drug effects Sequence Analysis, DNA sexual reproduction TATA Box Uhmfa1 gene Uhmfa2 gene Uhpra2 gene Ustilago - cytology Ustilago - drug effects Ustilago - genetics Ustilago hordei Ustilago segetum |
| title | Molecular analysis of the pheromone and pheromone receptor genes of Ustilago hordei |
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