Cloning, genomic organization and expression of a putative human transmembrane protein related to the Caenorhabditis elegans M01F1.4 gene
A novel human transcript CG-2 (C9ORF5), was isolated from the familial dysautonomia candidate region on 9q31 using a combination of cDNA selection and exon trapping. CG-2 was detected as a relatively abundant 8kb transcript in all adult and fetal tissues with the exception of adult thymus. Genomic a...
Gespeichert in:
| Veröffentlicht in: | Gene 1999-11, Vol.240 (1), p.67-73 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 73 |
|---|---|
| container_issue | 1 |
| container_start_page | 67 |
| container_title | Gene |
| container_volume | 240 |
| creator | Chadwick, B P Gill, S Leyne, M Mull, J Liebert, C B Robbins, C M Pinkett, H W Makalowska, I Maayan, C Blumenfeld, A Axelrod, F B Brownstein, M Slaugenhaupt, S A |
| description | A novel human transcript CG-2 (C9ORF5), was isolated from the familial dysautonomia candidate region on 9q31 using a combination of cDNA selection and exon trapping. CG-2 was detected as a relatively abundant 8kb transcript in all adult and fetal tissues with the exception of adult thymus. Genomic analysis of CG-2 identified 18 exons that span more than 110kb. The gene encodes a 911-amino-acid protein with a predicted molecular weight of 101kDa and a hypothetical pI of 9.03. Sequence analysis of CG-2 indicates that it is likely to encode a transmembrane protein. Here, we assess CG-2 as a candidate for familial dysautonomia. |
| doi_str_mv | 10.1016/S0378-1119(99)00432-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69285214</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17403299</sourcerecordid><originalsourceid>FETCH-LOGICAL-c336t-ea0fe1fd5e855c66c3e954178aec10463dd11aee8ffca9f5b2bea438df7cd3eb3</originalsourceid><addsrcrecordid>eNqFkc9uFDEMxnMooqXwCFQ5IZA6JZ5MZidHtKJ_pCIOwDnKJM5u0EyyTTKo8Aa8dbPdquKGL5btz_4s_Qh5C-wCGPQfvzG-GhoAkO-l_MBYx9sGjsjJc_uYvMr5J6shRPuSHAMTfTcAPyF_11MMPmzO6QZDnL2hMW108H908TFQHSzF-13CnPdldFTT3VLq8BfS7TLrQEvSIc84jzUj3aVY0AeacNIFLS2Rli3Sta7X01aP1hefKU5YTTL9wuASLrq9N74mL5yeMr55yqfkx-Xn7-vr5vbr1c36021jOO9Lg5o5BGcFDkKYvjccpehgNWg0wLqeWwugEQfnjJZOjO2IuuODdStjOY78lLw73K2v3i2Yi5p9NjhN9f24ZNXLdhAtdP8VwqpjvJWyCsVBaFLMOaFTu-RnnX4rYGoPSD0CUnsSSkr1CEhB3Tt7MljGGe0_Wwc6_AHV75EX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17403299</pqid></control><display><type>article</type><title>Cloning, genomic organization and expression of a putative human transmembrane protein related to the Caenorhabditis elegans M01F1.4 gene</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Chadwick, B P ; Gill, S ; Leyne, M ; Mull, J ; Liebert, C B ; Robbins, C M ; Pinkett, H W ; Makalowska, I ; Maayan, C ; Blumenfeld, A ; Axelrod, F B ; Brownstein, M ; Slaugenhaupt, S A</creator><creatorcontrib>Chadwick, B P ; Gill, S ; Leyne, M ; Mull, J ; Liebert, C B ; Robbins, C M ; Pinkett, H W ; Makalowska, I ; Maayan, C ; Blumenfeld, A ; Axelrod, F B ; Brownstein, M ; Slaugenhaupt, S A</creatorcontrib><description>A novel human transcript CG-2 (C9ORF5), was isolated from the familial dysautonomia candidate region on 9q31 using a combination of cDNA selection and exon trapping. CG-2 was detected as a relatively abundant 8kb transcript in all adult and fetal tissues with the exception of adult thymus. Genomic analysis of CG-2 identified 18 exons that span more than 110kb. The gene encodes a 911-amino-acid protein with a predicted molecular weight of 101kDa and a hypothetical pI of 9.03. Sequence analysis of CG-2 indicates that it is likely to encode a transmembrane protein. Here, we assess CG-2 as a candidate for familial dysautonomia.</description><identifier>ISSN: 0378-1119</identifier><identifier>DOI: 10.1016/S0378-1119(99)00432-1</identifier><identifier>PMID: 10564813</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Adult ; Amino Acid Sequence ; Animals ; Brain - embryology ; Brain - metabolism ; Caenorhabditis elegans ; Caenorhabditis elegans - genetics ; Cell Line ; CG-2 gene ; Chromosome Mapping ; Chromosomes, Human, Pair 9 - genetics ; Cloning, Molecular ; Cricetinae ; Databases, Factual ; DNA - chemistry ; DNA - genetics ; DNA Mutational Analysis ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; DNA, Complementary - isolation & purification ; dysautonomia ; Dysautonomia, Familial - genetics ; Expressed Sequence Tags ; Gene Expression ; Gene Expression Regulation, Developmental ; Genes - genetics ; Genes, Helminth - genetics ; Humans ; Hybrid Cells ; M01F1.4 gene ; Membrane Proteins - genetics ; Mice ; Molecular Sequence Data ; Rats ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid</subject><ispartof>Gene, 1999-11, Vol.240 (1), p.67-73</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c336t-ea0fe1fd5e855c66c3e954178aec10463dd11aee8ffca9f5b2bea438df7cd3eb3</citedby><cites>FETCH-LOGICAL-c336t-ea0fe1fd5e855c66c3e954178aec10463dd11aee8ffca9f5b2bea438df7cd3eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10564813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chadwick, B P</creatorcontrib><creatorcontrib>Gill, S</creatorcontrib><creatorcontrib>Leyne, M</creatorcontrib><creatorcontrib>Mull, J</creatorcontrib><creatorcontrib>Liebert, C B</creatorcontrib><creatorcontrib>Robbins, C M</creatorcontrib><creatorcontrib>Pinkett, H W</creatorcontrib><creatorcontrib>Makalowska, I</creatorcontrib><creatorcontrib>Maayan, C</creatorcontrib><creatorcontrib>Blumenfeld, A</creatorcontrib><creatorcontrib>Axelrod, F B</creatorcontrib><creatorcontrib>Brownstein, M</creatorcontrib><creatorcontrib>Slaugenhaupt, S A</creatorcontrib><title>Cloning, genomic organization and expression of a putative human transmembrane protein related to the Caenorhabditis elegans M01F1.4 gene</title><title>Gene</title><addtitle>Gene</addtitle><description>A novel human transcript CG-2 (C9ORF5), was isolated from the familial dysautonomia candidate region on 9q31 using a combination of cDNA selection and exon trapping. CG-2 was detected as a relatively abundant 8kb transcript in all adult and fetal tissues with the exception of adult thymus. Genomic analysis of CG-2 identified 18 exons that span more than 110kb. The gene encodes a 911-amino-acid protein with a predicted molecular weight of 101kDa and a hypothetical pI of 9.03. Sequence analysis of CG-2 indicates that it is likely to encode a transmembrane protein. Here, we assess CG-2 as a candidate for familial dysautonomia.</description><subject>Adult</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Brain - embryology</subject><subject>Brain - metabolism</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Cell Line</subject><subject>CG-2 gene</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 9 - genetics</subject><subject>Cloning, Molecular</subject><subject>Cricetinae</subject><subject>Databases, Factual</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA Mutational Analysis</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - isolation & purification</subject><subject>dysautonomia</subject><subject>Dysautonomia, Familial - genetics</subject><subject>Expressed Sequence Tags</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes - genetics</subject><subject>Genes, Helminth - genetics</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>M01F1.4 gene</subject><subject>Membrane Proteins - genetics</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Rats</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><issn>0378-1119</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9uFDEMxnMooqXwCFQ5IZA6JZ5MZidHtKJ_pCIOwDnKJM5u0EyyTTKo8Aa8dbPdquKGL5btz_4s_Qh5C-wCGPQfvzG-GhoAkO-l_MBYx9sGjsjJc_uYvMr5J6shRPuSHAMTfTcAPyF_11MMPmzO6QZDnL2hMW108H908TFQHSzF-13CnPdldFTT3VLq8BfS7TLrQEvSIc84jzUj3aVY0AeacNIFLS2Rli3Sta7X01aP1hefKU5YTTL9wuASLrq9N74mL5yeMr55yqfkx-Xn7-vr5vbr1c36021jOO9Lg5o5BGcFDkKYvjccpehgNWg0wLqeWwugEQfnjJZOjO2IuuODdStjOY78lLw73K2v3i2Yi5p9NjhN9f24ZNXLdhAtdP8VwqpjvJWyCsVBaFLMOaFTu-RnnX4rYGoPSD0CUnsSSkr1CEhB3Tt7MljGGe0_Wwc6_AHV75EX</recordid><startdate>19991115</startdate><enddate>19991115</enddate><creator>Chadwick, B P</creator><creator>Gill, S</creator><creator>Leyne, M</creator><creator>Mull, J</creator><creator>Liebert, C B</creator><creator>Robbins, C M</creator><creator>Pinkett, H W</creator><creator>Makalowska, I</creator><creator>Maayan, C</creator><creator>Blumenfeld, A</creator><creator>Axelrod, F B</creator><creator>Brownstein, M</creator><creator>Slaugenhaupt, S A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19991115</creationdate><title>Cloning, genomic organization and expression of a putative human transmembrane protein related to the Caenorhabditis elegans M01F1.4 gene</title><author>Chadwick, B P ; Gill, S ; Leyne, M ; Mull, J ; Liebert, C B ; Robbins, C M ; Pinkett, H W ; Makalowska, I ; Maayan, C ; Blumenfeld, A ; Axelrod, F B ; Brownstein, M ; Slaugenhaupt, S A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c336t-ea0fe1fd5e855c66c3e954178aec10463dd11aee8ffca9f5b2bea438df7cd3eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Brain - embryology</topic><topic>Brain - metabolism</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Cell Line</topic><topic>CG-2 gene</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 9 - genetics</topic><topic>Cloning, Molecular</topic><topic>Cricetinae</topic><topic>Databases, Factual</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA Mutational Analysis</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - isolation & purification</topic><topic>dysautonomia</topic><topic>Dysautonomia, Familial - genetics</topic><topic>Expressed Sequence Tags</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes - genetics</topic><topic>Genes, Helminth - genetics</topic><topic>Humans</topic><topic>Hybrid Cells</topic><topic>M01F1.4 gene</topic><topic>Membrane Proteins - genetics</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Rats</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chadwick, B P</creatorcontrib><creatorcontrib>Gill, S</creatorcontrib><creatorcontrib>Leyne, M</creatorcontrib><creatorcontrib>Mull, J</creatorcontrib><creatorcontrib>Liebert, C B</creatorcontrib><creatorcontrib>Robbins, C M</creatorcontrib><creatorcontrib>Pinkett, H W</creatorcontrib><creatorcontrib>Makalowska, I</creatorcontrib><creatorcontrib>Maayan, C</creatorcontrib><creatorcontrib>Blumenfeld, A</creatorcontrib><creatorcontrib>Axelrod, F B</creatorcontrib><creatorcontrib>Brownstein, M</creatorcontrib><creatorcontrib>Slaugenhaupt, S A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chadwick, B P</au><au>Gill, S</au><au>Leyne, M</au><au>Mull, J</au><au>Liebert, C B</au><au>Robbins, C M</au><au>Pinkett, H W</au><au>Makalowska, I</au><au>Maayan, C</au><au>Blumenfeld, A</au><au>Axelrod, F B</au><au>Brownstein, M</au><au>Slaugenhaupt, S A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning, genomic organization and expression of a putative human transmembrane protein related to the Caenorhabditis elegans M01F1.4 gene</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1999-11-15</date><risdate>1999</risdate><volume>240</volume><issue>1</issue><spage>67</spage><epage>73</epage><pages>67-73</pages><issn>0378-1119</issn><abstract>A novel human transcript CG-2 (C9ORF5), was isolated from the familial dysautonomia candidate region on 9q31 using a combination of cDNA selection and exon trapping. CG-2 was detected as a relatively abundant 8kb transcript in all adult and fetal tissues with the exception of adult thymus. Genomic analysis of CG-2 identified 18 exons that span more than 110kb. The gene encodes a 911-amino-acid protein with a predicted molecular weight of 101kDa and a hypothetical pI of 9.03. Sequence analysis of CG-2 indicates that it is likely to encode a transmembrane protein. Here, we assess CG-2 as a candidate for familial dysautonomia.</abstract><cop>Netherlands</cop><pmid>10564813</pmid><doi>10.1016/S0378-1119(99)00432-1</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1999-11, Vol.240 (1), p.67-73 |
| issn | 0378-1119 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69285214 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Amino Acid Sequence Animals Brain - embryology Brain - metabolism Caenorhabditis elegans Caenorhabditis elegans - genetics Cell Line CG-2 gene Chromosome Mapping Chromosomes, Human, Pair 9 - genetics Cloning, Molecular Cricetinae Databases, Factual DNA - chemistry DNA - genetics DNA Mutational Analysis DNA, Complementary - chemistry DNA, Complementary - genetics DNA, Complementary - isolation & purification dysautonomia Dysautonomia, Familial - genetics Expressed Sequence Tags Gene Expression Gene Expression Regulation, Developmental Genes - genetics Genes, Helminth - genetics Humans Hybrid Cells M01F1.4 gene Membrane Proteins - genetics Mice Molecular Sequence Data Rats Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid |
| title | Cloning, genomic organization and expression of a putative human transmembrane protein related to the Caenorhabditis elegans M01F1.4 gene |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T09%3A50%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning,%20genomic%20organization%20and%20expression%20of%20a%20putative%20human%20transmembrane%20protein%20related%20to%20the%20Caenorhabditis%20elegans%20M01F1.4%20gene&rft.jtitle=Gene&rft.au=Chadwick,%20B%20P&rft.date=1999-11-15&rft.volume=240&rft.issue=1&rft.spage=67&rft.epage=73&rft.pages=67-73&rft.issn=0378-1119&rft_id=info:doi/10.1016/S0378-1119(99)00432-1&rft_dat=%3Cproquest_cross%3E17403299%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17403299&rft_id=info:pmid/10564813&rfr_iscdi=true |