Validation of a Novel Estrogen Receptor-Based Microtitration Plate Assay for the Determination of Phytoestrogens in Soy-Based Foods
A novel, nonisotopic microtitration plate assay based on the human estrogen receptor has been used to screen soy-based and soy-containing foods for their phytoestrogen content (measured as genistein equivalents). The validation of the assay for use with food extracts has been demonstrated by investi...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 1999-10, Vol.47 (10), p.4106-4111 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Garrett, Stephen D Lee, Heather A Friar, Phöbe M. K Morgan, Michael R. A |
| description | A novel, nonisotopic microtitration plate assay based on the human estrogen receptor has been used to screen soy-based and soy-containing foods for their phytoestrogen content (measured as genistein equivalents). The validation of the assay for use with food extracts has been demonstrated by investigation of recoveries after acidic and enzymic hydrolysis, by investigation of matrix effects, and by comparison of results with HPLC analysis. Phytoestrogen levels in soy products analyzed ranged between 520 and 1872 μg of genistein equiv/g of soy flour, 5−282 μg/g of soy concentrates, 503−1292 μg/g of soy-protein isolates, and 108−226 μg/g of soy-based infant formulas. Samples of textured vegetable protein and bread containing soy and linseed gave values of 1114 and 68 μg/g, respectively. Comparison of results for 12 samples analyzed both by the receptor assay and by HPLC showed good correlation (r 2 = 0.905). The assay, which is rapid and simple to perform, is suitable for screening phytoestrogen-containing foods in order to assess human exposure to these bioactive compounds. The assay sensitivity is 3.4 μg/g, and 14 samples/plate can be analyzed in 4 h following hydrolysis. Keywords: Phytoestrogens; estrogenic isoflavonoids; receptor assay; screening; soy-based food |
| doi_str_mv | 10.1021/jf990579o |
| format | Article |
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K ; Morgan, Michael R. A</creator><creatorcontrib>Garrett, Stephen D ; Lee, Heather A ; Friar, Phöbe M. K ; Morgan, Michael R. A</creatorcontrib><description>A novel, nonisotopic microtitration plate assay based on the human estrogen receptor has been used to screen soy-based and soy-containing foods for their phytoestrogen content (measured as genistein equivalents). The validation of the assay for use with food extracts has been demonstrated by investigation of recoveries after acidic and enzymic hydrolysis, by investigation of matrix effects, and by comparison of results with HPLC analysis. Phytoestrogen levels in soy products analyzed ranged between 520 and 1872 μg of genistein equiv/g of soy flour, 5−282 μg/g of soy concentrates, 503−1292 μg/g of soy-protein isolates, and 108−226 μg/g of soy-based infant formulas. Samples of textured vegetable protein and bread containing soy and linseed gave values of 1114 and 68 μg/g, respectively. Comparison of results for 12 samples analyzed both by the receptor assay and by HPLC showed good correlation (r 2 = 0.905). The assay, which is rapid and simple to perform, is suitable for screening phytoestrogen-containing foods in order to assess human exposure to these bioactive compounds. The assay sensitivity is 3.4 μg/g, and 14 samples/plate can be analyzed in 4 h following hydrolysis. Keywords: Phytoestrogens; estrogenic isoflavonoids; receptor assay; screening; soy-based food</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf990579o</identifier><identifier>PMID: 10552774</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>assays ; Biological and medical sciences ; breads ; Chromatography, High Pressure Liquid ; Enzyme-Linked Immunosorbent Assay ; estrogen receptors ; estrogenic properties ; Estrogens, Non-Steroidal - isolation & purification ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; General pharmacology ; genistein ; Genistein - isolation & purification ; Glycine max - chemistry ; Humans ; infant formulas ; Isoflavones ; isoflavonoids ; lignans ; linseed ; meat analogs ; Medical sciences ; Methods of analysis, processing and quality control, regulation, standards ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Phytoestrogens ; plant estrogens ; Plant Preparations ; protein isolates ; Receptors, Estrogen ; Research and development. New food products, dietetic foods and beverages ; Sensitivity and Specificity ; soy flour ; soy protein ; soybeans</subject><ispartof>Journal of agricultural and food chemistry, 1999-10, Vol.47 (10), p.4106-4111</ispartof><rights>Copyright © 1999 American Chemical Society</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a402t-5fdf760db047f444d1c249e4c030ebe9b0040eb992b5534d931846f011ab6b393</citedby><cites>FETCH-LOGICAL-a402t-5fdf760db047f444d1c249e4c030ebe9b0040eb992b5534d931846f011ab6b393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf990579o$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf990579o$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1249416$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10552774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garrett, Stephen D</creatorcontrib><creatorcontrib>Lee, Heather A</creatorcontrib><creatorcontrib>Friar, Phöbe M. K</creatorcontrib><creatorcontrib>Morgan, Michael R. A</creatorcontrib><title>Validation of a Novel Estrogen Receptor-Based Microtitration Plate Assay for the Determination of Phytoestrogens in Soy-Based Foods</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>A novel, nonisotopic microtitration plate assay based on the human estrogen receptor has been used to screen soy-based and soy-containing foods for their phytoestrogen content (measured as genistein equivalents). The validation of the assay for use with food extracts has been demonstrated by investigation of recoveries after acidic and enzymic hydrolysis, by investigation of matrix effects, and by comparison of results with HPLC analysis. Phytoestrogen levels in soy products analyzed ranged between 520 and 1872 μg of genistein equiv/g of soy flour, 5−282 μg/g of soy concentrates, 503−1292 μg/g of soy-protein isolates, and 108−226 μg/g of soy-based infant formulas. Samples of textured vegetable protein and bread containing soy and linseed gave values of 1114 and 68 μg/g, respectively. Comparison of results for 12 samples analyzed both by the receptor assay and by HPLC showed good correlation (r 2 = 0.905). The assay, which is rapid and simple to perform, is suitable for screening phytoestrogen-containing foods in order to assess human exposure to these bioactive compounds. The assay sensitivity is 3.4 μg/g, and 14 samples/plate can be analyzed in 4 h following hydrolysis. Keywords: Phytoestrogens; estrogenic isoflavonoids; receptor assay; screening; soy-based food</description><subject>assays</subject><subject>Biological and medical sciences</subject><subject>breads</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>estrogen receptors</subject><subject>estrogenic properties</subject><subject>Estrogens, Non-Steroidal - isolation & purification</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General pharmacology</subject><subject>genistein</subject><subject>Genistein - isolation & purification</subject><subject>Glycine max - chemistry</subject><subject>Humans</subject><subject>infant formulas</subject><subject>Isoflavones</subject><subject>isoflavonoids</subject><subject>lignans</subject><subject>linseed</subject><subject>meat analogs</subject><subject>Medical sciences</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Phytoestrogens</subject><subject>plant estrogens</subject><subject>Plant Preparations</subject><subject>protein isolates</subject><subject>Receptors, Estrogen</subject><subject>Research and development. New food products, dietetic foods and beverages</subject><subject>Sensitivity and Specificity</subject><subject>soy flour</subject><subject>soy protein</subject><subject>soybeans</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0E-P1CAYBnBiNO7s6sEvoBzUxEP1hUIpx3X_mow6cWaNN0Jb2GW2U0ZgjHP2i4tpM3rwBMn74wnvg9AzAm8JUPJubaUELqR_gGaEUyg4IfVDNIM8LGpekSN0HOMaAGou4DE6IsA5FYLN0K-vunedTs4P2Fus8Sf_w_T4Iqbgb82Av5jWbJMPxXsdTYc_ujb45FIYXyx6nQw-jVHvsfUBpzuDz00yYeOGQ-bibp-8mQIjdgNe-v2Ud-l9F5-gR1b30TydzhN0c3mxOrsu5p-vPpydzgvNgKaC286KCroGmLCMsY60lEnDWijBNEY2ACxfpKQN5yXrZElqVlkgRDdVU8ryBL0ec7fBf9_lH6mNi63pez0Yv4uqkrRmUogM34wwLxtjMFZtg9vosFcE1J_G1aHxbJ9PobtmY7p_5FhxBi8noGOrexv00Lr41-UdGKkyK0bmYjI_D2Md7lUlSsHVarFU83J1df1tQdQy-xejt9orfRty5M2SAimBSk4Zq7N4NQrdRrX2uzDkcv-zwW8Ax62q</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Garrett, Stephen D</creator><creator>Lee, Heather A</creator><creator>Friar, Phöbe M. K</creator><creator>Morgan, Michael R. A</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Validation of a Novel Estrogen Receptor-Based Microtitration Plate Assay for the Determination of Phytoestrogens in Soy-Based Foods</title><author>Garrett, Stephen D ; Lee, Heather A ; Friar, Phöbe M. K ; Morgan, Michael R. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a402t-5fdf760db047f444d1c249e4c030ebe9b0040eb992b5534d931846f011ab6b393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>assays</topic><topic>Biological and medical sciences</topic><topic>breads</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>estrogen receptors</topic><topic>estrogenic properties</topic><topic>Estrogens, Non-Steroidal - isolation & purification</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General pharmacology</topic><topic>genistein</topic><topic>Genistein - isolation & purification</topic><topic>Glycine max - chemistry</topic><topic>Humans</topic><topic>infant formulas</topic><topic>Isoflavones</topic><topic>isoflavonoids</topic><topic>lignans</topic><topic>linseed</topic><topic>meat analogs</topic><topic>Medical sciences</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Phytoestrogens</topic><topic>plant estrogens</topic><topic>Plant Preparations</topic><topic>protein isolates</topic><topic>Receptors, Estrogen</topic><topic>Research and development. New food products, dietetic foods and beverages</topic><topic>Sensitivity and Specificity</topic><topic>soy flour</topic><topic>soy protein</topic><topic>soybeans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garrett, Stephen D</creatorcontrib><creatorcontrib>Lee, Heather A</creatorcontrib><creatorcontrib>Friar, Phöbe M. K</creatorcontrib><creatorcontrib>Morgan, Michael R. A</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garrett, Stephen D</au><au>Lee, Heather A</au><au>Friar, Phöbe M. K</au><au>Morgan, Michael R. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validation of a Novel Estrogen Receptor-Based Microtitration Plate Assay for the Determination of Phytoestrogens in Soy-Based Foods</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>47</volume><issue>10</issue><spage>4106</spage><epage>4111</epage><pages>4106-4111</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>A novel, nonisotopic microtitration plate assay based on the human estrogen receptor has been used to screen soy-based and soy-containing foods for their phytoestrogen content (measured as genistein equivalents). The validation of the assay for use with food extracts has been demonstrated by investigation of recoveries after acidic and enzymic hydrolysis, by investigation of matrix effects, and by comparison of results with HPLC analysis. Phytoestrogen levels in soy products analyzed ranged between 520 and 1872 μg of genistein equiv/g of soy flour, 5−282 μg/g of soy concentrates, 503−1292 μg/g of soy-protein isolates, and 108−226 μg/g of soy-based infant formulas. Samples of textured vegetable protein and bread containing soy and linseed gave values of 1114 and 68 μg/g, respectively. Comparison of results for 12 samples analyzed both by the receptor assay and by HPLC showed good correlation (r 2 = 0.905). The assay, which is rapid and simple to perform, is suitable for screening phytoestrogen-containing foods in order to assess human exposure to these bioactive compounds. The assay sensitivity is 3.4 μg/g, and 14 samples/plate can be analyzed in 4 h following hydrolysis. Keywords: Phytoestrogens; estrogenic isoflavonoids; receptor assay; screening; soy-based food</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>10552774</pmid><doi>10.1021/jf990579o</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 1999-10, Vol.47 (10), p.4106-4111 |
| issn | 0021-8561 1520-5118 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | assays Biological and medical sciences breads Chromatography, High Pressure Liquid Enzyme-Linked Immunosorbent Assay estrogen receptors estrogenic properties Estrogens, Non-Steroidal - isolation & purification Food industries Fundamental and applied biological sciences. Psychology General aspects General pharmacology genistein Genistein - isolation & purification Glycine max - chemistry Humans infant formulas Isoflavones isoflavonoids lignans linseed meat analogs Medical sciences Methods of analysis, processing and quality control, regulation, standards Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Phytoestrogens plant estrogens Plant Preparations protein isolates Receptors, Estrogen Research and development. New food products, dietetic foods and beverages Sensitivity and Specificity soy flour soy protein soybeans |
| title | Validation of a Novel Estrogen Receptor-Based Microtitration Plate Assay for the Determination of Phytoestrogens in Soy-Based Foods |
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