Validation of a competitive ELISA for diagnosis of Brucella melitensis infection in sheep and goats

A competitive enzyme-linked immunosorbent assay (cELISA) was validated for the serodiagnosis of Brucella melitensis infection in small ruminants using 2108 positive and 2154 negative reference sera from sheep and goats. The optimum cut-off values, offering the highest diagnostic sensitivity (DSn) an...

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Veröffentlicht in:	The veterinary journal (1997) 2008-09, Vol.177 (3), p.411-417
Hauptverfasser:	Minas, Anastasios, Stournara, Athanasia, Christodoulopoulos, Georgios, Katsoulos, Panagiotis D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Bacterial - blood Brucella melitensis Brucella melitensis - immunology brucellosis Brucellosis - blood Brucellosis - diagnosis Brucellosis - veterinary Competitive ELISA competitive enzyme-linked immunosorbent assay complement fixation tests diagnostic techniques disease diagnosis enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - standards Enzyme-Linked Immunosorbent Assay - veterinary goat diseases Goat Diseases - blood Goat Diseases - diagnosis Goats Reference Values Reproducibility of Results ROC Curve Sensitivity and Specificity serodiagnosis Serology Sheep sheep diseases Sheep Diseases - blood Sheep Diseases - diagnosis test sensitivity test specificity Validation
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