Transcription-dependent spatial arrangements of CFTR and conserved adjacent loci are not conserved in human and murine nuclei
The human genes CFTR, ASZ1/GASZ, and CTTNBP2/CORTBP2 map to adjacent loci on chromosome 7q31 and display characteristic patterns of nuclear positioning, which strictly correlate with the state of activity. To address the evolutionary conservation of gene positioning, we investigated transcriptional...
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| Veröffentlicht in: | Chromosoma 2008-08, Vol.117 (4), p.381-397 |
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| creator | Sadoni, Nicolas Targosz, Bianca-Sabrina Englmann, Andreas Fesser, Stephanie Koch, Jeannette Schindelhauer, Dirk Zink, Daniele |
| description | The human genes CFTR, ASZ1/GASZ, and CTTNBP2/CORTBP2 map to adjacent loci on chromosome 7q31 and display characteristic patterns of nuclear positioning, which strictly correlate with the state of activity. To address the evolutionary conservation of gene positioning, we investigated transcriptional activity and nuclear positioning of the highly conserved murine orthologs and of additional murine genes mapping to the region of conserved synteny on mouse chromosome 6. The results showed that all murine loci investigated constitutively localized in the nuclear interior irrespective of their functional state. Silenced loci did not display preferential association with the nuclear periphery or with chromocenters, respectively, and no differential positioning with respect to the chromosome 6 territory could be observed. This positional behavior of the murine loci was in striking contrast to the positioning of the human orthologs, and the results show that the transcription-dependent positioning of CFTR and adjacent loci has not been conserved. The findings reveal that the nuclear organization of conserved chromosomal regions can change rapidly during evolution and is not always as highly conserved as other features of chromosome organization. Furthermore, the results suggest that the way how nuclear positioning contributes to the regulation of conserved loci can be different in different vertebrate species. |
| doi_str_mv | 10.1007/s00412-008-0157-5 |
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To address the evolutionary conservation of gene positioning, we investigated transcriptional activity and nuclear positioning of the highly conserved murine orthologs and of additional murine genes mapping to the region of conserved synteny on mouse chromosome 6. The results showed that all murine loci investigated constitutively localized in the nuclear interior irrespective of their functional state. Silenced loci did not display preferential association with the nuclear periphery or with chromocenters, respectively, and no differential positioning with respect to the chromosome 6 territory could be observed. This positional behavior of the murine loci was in striking contrast to the positioning of the human orthologs, and the results show that the transcription-dependent positioning of CFTR and adjacent loci has not been conserved. The findings reveal that the nuclear organization of conserved chromosomal regions can change rapidly during evolution and is not always as highly conserved as other features of chromosome organization. Furthermore, the results suggest that the way how nuclear positioning contributes to the regulation of conserved loci can be different in different vertebrate species.</description><identifier>ISSN: 0009-5915</identifier><identifier>EISSN: 1432-0886</identifier><identifier>DOI: 10.1007/s00412-008-0157-5</identifier><identifier>PMID: 18408947</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Animal Genetics and Genomics ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; Cell Line ; Cell Nucleus - genetics ; Chromosomes, Artificial, Bacterial ; Chromosomes, Human, Pair 7 - genetics ; Cystic Fibrosis Transmembrane Conductance Regulator - genetics ; Developmental Biology ; DNA Primers - genetics ; Eukaryotic Microbiology ; Gene Expression Regulation - genetics ; Human Genetics ; Humans ; In Situ Hybridization, Fluorescence ; Life Sciences ; Mice ; Research Article ; Reverse Transcriptase Polymerase Chain Reaction ; Species Specificity ; Synteny - genetics</subject><ispartof>Chromosoma, 2008-08, Vol.117 (4), p.381-397</ispartof><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-26edea013a1e8312a089785c8b924b6c8447088665b66f1d54669b19bc95fad83</citedby><cites>FETCH-LOGICAL-c393t-26edea013a1e8312a089785c8b924b6c8447088665b66f1d54669b19bc95fad83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00412-008-0157-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00412-008-0157-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18408947$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sadoni, Nicolas</creatorcontrib><creatorcontrib>Targosz, Bianca-Sabrina</creatorcontrib><creatorcontrib>Englmann, Andreas</creatorcontrib><creatorcontrib>Fesser, Stephanie</creatorcontrib><creatorcontrib>Koch, Jeannette</creatorcontrib><creatorcontrib>Schindelhauer, Dirk</creatorcontrib><creatorcontrib>Zink, Daniele</creatorcontrib><title>Transcription-dependent spatial arrangements of CFTR and conserved adjacent loci are not conserved in human and murine nuclei</title><title>Chromosoma</title><addtitle>Chromosoma</addtitle><addtitle>Chromosoma</addtitle><description>The human genes CFTR, ASZ1/GASZ, and CTTNBP2/CORTBP2 map to adjacent loci on chromosome 7q31 and display characteristic patterns of nuclear positioning, which strictly correlate with the state of activity. To address the evolutionary conservation of gene positioning, we investigated transcriptional activity and nuclear positioning of the highly conserved murine orthologs and of additional murine genes mapping to the region of conserved synteny on mouse chromosome 6. The results showed that all murine loci investigated constitutively localized in the nuclear interior irrespective of their functional state. Silenced loci did not display preferential association with the nuclear periphery or with chromocenters, respectively, and no differential positioning with respect to the chromosome 6 territory could be observed. This positional behavior of the murine loci was in striking contrast to the positioning of the human orthologs, and the results show that the transcription-dependent positioning of CFTR and adjacent loci has not been conserved. The findings reveal that the nuclear organization of conserved chromosomal regions can change rapidly during evolution and is not always as highly conserved as other features of chromosome organization. Furthermore, the results suggest that the way how nuclear positioning contributes to the regulation of conserved loci can be different in different vertebrate species.</description><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cell Line</subject><subject>Cell Nucleus - genetics</subject><subject>Chromosomes, Artificial, Bacterial</subject><subject>Chromosomes, Human, Pair 7 - genetics</subject><subject>Cystic Fibrosis Transmembrane Conductance Regulator - genetics</subject><subject>Developmental Biology</subject><subject>DNA Primers - genetics</subject><subject>Eukaryotic Microbiology</subject><subject>Gene Expression Regulation - genetics</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Research Article</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Species Specificity</subject><subject>Synteny - 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Academic</collection><jtitle>Chromosoma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sadoni, Nicolas</au><au>Targosz, Bianca-Sabrina</au><au>Englmann, Andreas</au><au>Fesser, Stephanie</au><au>Koch, Jeannette</au><au>Schindelhauer, Dirk</au><au>Zink, Daniele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcription-dependent spatial arrangements of CFTR and conserved adjacent loci are not conserved in human and murine nuclei</atitle><jtitle>Chromosoma</jtitle><stitle>Chromosoma</stitle><addtitle>Chromosoma</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>117</volume><issue>4</issue><spage>381</spage><epage>397</epage><pages>381-397</pages><issn>0009-5915</issn><eissn>1432-0886</eissn><abstract>The human genes CFTR, ASZ1/GASZ, and CTTNBP2/CORTBP2 map to adjacent loci on chromosome 7q31 and display characteristic patterns of nuclear positioning, which strictly correlate with the state of activity. To address the evolutionary conservation of gene positioning, we investigated transcriptional activity and nuclear positioning of the highly conserved murine orthologs and of additional murine genes mapping to the region of conserved synteny on mouse chromosome 6. The results showed that all murine loci investigated constitutively localized in the nuclear interior irrespective of their functional state. Silenced loci did not display preferential association with the nuclear periphery or with chromocenters, respectively, and no differential positioning with respect to the chromosome 6 territory could be observed. This positional behavior of the murine loci was in striking contrast to the positioning of the human orthologs, and the results show that the transcription-dependent positioning of CFTR and adjacent loci has not been conserved. 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| ispartof | Chromosoma, 2008-08, Vol.117 (4), p.381-397 |
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| subjects | Animal Genetics and Genomics Animals Biochemistry Biomedical and Life Sciences Cell Biology Cell Line Cell Nucleus - genetics Chromosomes, Artificial, Bacterial Chromosomes, Human, Pair 7 - genetics Cystic Fibrosis Transmembrane Conductance Regulator - genetics Developmental Biology DNA Primers - genetics Eukaryotic Microbiology Gene Expression Regulation - genetics Human Genetics Humans In Situ Hybridization, Fluorescence Life Sciences Mice Research Article Reverse Transcriptase Polymerase Chain Reaction Species Specificity Synteny - genetics |
| title | Transcription-dependent spatial arrangements of CFTR and conserved adjacent loci are not conserved in human and murine nuclei |
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