Interphase Fluorescence In Situ Hybridization Overcomes Pitfalls of G-Banding Analysis with Special Reference to Underestimation of Chromosomal Aberration Rates
Fluorescence in situ hybridization (FISH) is suitable for detecting different types of chromosome aberrations on interphase nuclei even in specimens with no or few chromosome metaphases. However, it is not known why FISH is superior to conventional G-banding analysis. The sensitivity of interphase F...
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| Veröffentlicht in: | Cancer genetics and cytogenetics 1999-11, Vol.115 (1), p.32-38 |
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| container_title | Cancer genetics and cytogenetics |
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| creator | Tanaka, Kimio Arif, Mansyur Eguchi, Mariko Shintani, Takahiro Kumaravel, Tirukalikundram S. Asaoku, Hiroki Kyo, Taichi Dohy, Hiroo Kamada, Nanao |
| description | Fluorescence in situ hybridization (FISH) is suitable for detecting different types of chromosome aberrations on interphase nuclei even in specimens with no or few chromosome metaphases. However, it is not known why FISH is superior to conventional G-banding analysis. The sensitivity of interphase FISH was compared to that of G-banding analysis in 288 leukemia/lymphoma patients for 10 different types of chromosome aberrations: t(9;22) (M- and m-BCR), t(8;21), 11q23 abnormalities, t(15;17), del(5)/
−5, del(13)/
−13,
+8,
−7, and
+12. The results revealed that t(15;17) positive cells could not proliferate well in culture, leading to underestimation of abnormality by G-banding. Monosomy 7 in acute myelocytic leukemia (AML) and myelodysplastic syndrome (MDS) as well as trisomy 12 and deletion chromosome 13 in chronic lymphocytic leukemias (CLL) were also severely underestimated by G-banding. On the other hand, no discrepancies were observed in t(8;21), t(9;22), translations involving 11q23, or in trisomy 8. These findings indicate the superiority of interphase FISH over conventional cytogenetics for detecting chromosome abnormalities in small clones, especially for monosomy 7 or (15;17) translocations. |
| doi_str_mv | 10.1016/S0165-4608(99)00079-5 |
| format | Article |
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−5, del(13)/
−13,
+8,
−7, and
+12. The results revealed that t(15;17) positive cells could not proliferate well in culture, leading to underestimation of abnormality by G-banding. Monosomy 7 in acute myelocytic leukemia (AML) and myelodysplastic syndrome (MDS) as well as trisomy 12 and deletion chromosome 13 in chronic lymphocytic leukemias (CLL) were also severely underestimated by G-banding. On the other hand, no discrepancies were observed in t(8;21), t(9;22), translations involving 11q23, or in trisomy 8. These findings indicate the superiority of interphase FISH over conventional cytogenetics for detecting chromosome abnormalities in small clones, especially for monosomy 7 or (15;17) translocations.</description><identifier>ISSN: 0165-4608</identifier><identifier>EISSN: 1873-4456</identifier><identifier>DOI: 10.1016/S0165-4608(99)00079-5</identifier><identifier>PMID: 10565297</identifier><identifier>CODEN: CGCYDF</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Chromosome Aberrations ; Chromosome Banding - methods ; Gene Deletion ; Humans ; In Situ Hybridization, Fluorescence - methods ; Interphase ; Investigative techniques, diagnostic techniques (general aspects) ; Leukemia, Lymphocytic, Chronic, B-Cell - genetics ; Leukemia, Myeloid, Acute - genetics ; Medical sciences ; Metaphase ; Miscellaneous. Technology ; Monosomy ; Myelodysplastic Syndromes - genetics ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Translocation, Genetic ; Trisomy</subject><ispartof>Cancer genetics and cytogenetics, 1999-11, Vol.115 (1), p.32-38</ispartof><rights>1999 Elsevier Science Inc.</rights><rights>2000 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-2c07374017c9cc497d79784174878f114b43ecfc6264c8fed68ff1e28505348f3</citedby><cites>FETCH-LOGICAL-c390t-2c07374017c9cc497d79784174878f114b43ecfc6264c8fed68ff1e28505348f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0165-4608(99)00079-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1191943$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10565297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tanaka, Kimio</creatorcontrib><creatorcontrib>Arif, Mansyur</creatorcontrib><creatorcontrib>Eguchi, Mariko</creatorcontrib><creatorcontrib>Shintani, Takahiro</creatorcontrib><creatorcontrib>Kumaravel, Tirukalikundram S.</creatorcontrib><creatorcontrib>Asaoku, Hiroki</creatorcontrib><creatorcontrib>Kyo, Taichi</creatorcontrib><creatorcontrib>Dohy, Hiroo</creatorcontrib><creatorcontrib>Kamada, Nanao</creatorcontrib><title>Interphase Fluorescence In Situ Hybridization Overcomes Pitfalls of G-Banding Analysis with Special Reference to Underestimation of Chromosomal Aberration Rates</title><title>Cancer genetics and cytogenetics</title><addtitle>Cancer Genet Cytogenet</addtitle><description>Fluorescence in situ hybridization (FISH) is suitable for detecting different types of chromosome aberrations on interphase nuclei even in specimens with no or few chromosome metaphases. However, it is not known why FISH is superior to conventional G-banding analysis. The sensitivity of interphase FISH was compared to that of G-banding analysis in 288 leukemia/lymphoma patients for 10 different types of chromosome aberrations: t(9;22) (M- and m-BCR), t(8;21), 11q23 abnormalities, t(15;17), del(5)/
−5, del(13)/
−13,
+8,
−7, and
+12. The results revealed that t(15;17) positive cells could not proliferate well in culture, leading to underestimation of abnormality by G-banding. Monosomy 7 in acute myelocytic leukemia (AML) and myelodysplastic syndrome (MDS) as well as trisomy 12 and deletion chromosome 13 in chronic lymphocytic leukemias (CLL) were also severely underestimated by G-banding. On the other hand, no discrepancies were observed in t(8;21), t(9;22), translations involving 11q23, or in trisomy 8. These findings indicate the superiority of interphase FISH over conventional cytogenetics for detecting chromosome abnormalities in small clones, especially for monosomy 7 or (15;17) translocations.</description><subject>Biological and medical sciences</subject><subject>Chromosome Aberrations</subject><subject>Chromosome Banding - methods</subject><subject>Gene Deletion</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Interphase</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leukemia, Lymphocytic, Chronic, B-Cell - genetics</subject><subject>Leukemia, Myeloid, Acute - genetics</subject><subject>Medical sciences</subject><subject>Metaphase</subject><subject>Miscellaneous. Technology</subject><subject>Monosomy</subject><subject>Myelodysplastic Syndromes - genetics</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Translocation, Genetic</subject><subject>Trisomy</subject><issn>0165-4608</issn><issn>1873-4456</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdGOEyEUhonRuHX1ETRcGKMXozDDDHBlauPuNtlkzda9JpQ5WMwMdIGuqU_jo0o7jXonFxDCd_5z-H-EXlLynhLafViVra1YR8RbKd8RQris2kdoRgVvKsba7jGa_UHO0LOUvh-gWnZP0RklbdfWks_Qr6XPELcbnQBfDLsQIRnwBvDS45XLO3y1X0fXu586u-DxzQNEE0ZI-IvLVg9DwsHiy-qT9r3z3_Dc62GfXMI_XN7g1RaM0wO-BQvxqJoDvvN9uaTsxkmy1C82MYwhhbGw8zXEOL3c6gzpOXpS-iR4cTrP0d3F56-Lq-r65nK5mF9XppEkV7UhvOGMUG6kMUzynksuGOVMcGEpZWvWgLGmqztmhIW-E9ZSqEVL2oYJ25yjN5PuNob7XZlPja5YMQzaQ9gl1cla0LIK2E6giSGlCFZtY_lL3CtK1CEadYxGHXxXUqpjNKotda9ODXbrEfp_qqYsCvD6BOhk9GCj9salvxyVVLKmYB8nDIobDw6iSsYdzO1dBJNVH9x_JvkNbritPw</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>Tanaka, Kimio</creator><creator>Arif, Mansyur</creator><creator>Eguchi, Mariko</creator><creator>Shintani, Takahiro</creator><creator>Kumaravel, Tirukalikundram S.</creator><creator>Asaoku, Hiroki</creator><creator>Kyo, Taichi</creator><creator>Dohy, Hiroo</creator><creator>Kamada, Nanao</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991101</creationdate><title>Interphase Fluorescence In Situ Hybridization Overcomes Pitfalls of G-Banding Analysis with Special Reference to Underestimation of Chromosomal Aberration Rates</title><author>Tanaka, Kimio ; Arif, Mansyur ; Eguchi, Mariko ; Shintani, Takahiro ; Kumaravel, Tirukalikundram S. ; Asaoku, Hiroki ; Kyo, Taichi ; Dohy, Hiroo ; Kamada, Nanao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-2c07374017c9cc497d79784174878f114b43ecfc6264c8fed68ff1e28505348f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>Chromosome Aberrations</topic><topic>Chromosome Banding - methods</topic><topic>Gene Deletion</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Interphase</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Leukemia, Lymphocytic, Chronic, B-Cell - genetics</topic><topic>Leukemia, Myeloid, Acute - genetics</topic><topic>Medical sciences</topic><topic>Metaphase</topic><topic>Miscellaneous. Technology</topic><topic>Monosomy</topic><topic>Myelodysplastic Syndromes - genetics</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Translocation, Genetic</topic><topic>Trisomy</topic><toplevel>online_resources</toplevel><creatorcontrib>Tanaka, Kimio</creatorcontrib><creatorcontrib>Arif, Mansyur</creatorcontrib><creatorcontrib>Eguchi, Mariko</creatorcontrib><creatorcontrib>Shintani, Takahiro</creatorcontrib><creatorcontrib>Kumaravel, Tirukalikundram S.</creatorcontrib><creatorcontrib>Asaoku, Hiroki</creatorcontrib><creatorcontrib>Kyo, Taichi</creatorcontrib><creatorcontrib>Dohy, Hiroo</creatorcontrib><creatorcontrib>Kamada, Nanao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer genetics and cytogenetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanaka, Kimio</au><au>Arif, Mansyur</au><au>Eguchi, Mariko</au><au>Shintani, Takahiro</au><au>Kumaravel, Tirukalikundram S.</au><au>Asaoku, Hiroki</au><au>Kyo, Taichi</au><au>Dohy, Hiroo</au><au>Kamada, Nanao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interphase Fluorescence In Situ Hybridization Overcomes Pitfalls of G-Banding Analysis with Special Reference to Underestimation of Chromosomal Aberration Rates</atitle><jtitle>Cancer genetics and cytogenetics</jtitle><addtitle>Cancer Genet Cytogenet</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>115</volume><issue>1</issue><spage>32</spage><epage>38</epage><pages>32-38</pages><issn>0165-4608</issn><eissn>1873-4456</eissn><coden>CGCYDF</coden><abstract>Fluorescence in situ hybridization (FISH) is suitable for detecting different types of chromosome aberrations on interphase nuclei even in specimens with no or few chromosome metaphases. However, it is not known why FISH is superior to conventional G-banding analysis. The sensitivity of interphase FISH was compared to that of G-banding analysis in 288 leukemia/lymphoma patients for 10 different types of chromosome aberrations: t(9;22) (M- and m-BCR), t(8;21), 11q23 abnormalities, t(15;17), del(5)/
−5, del(13)/
−13,
+8,
−7, and
+12. The results revealed that t(15;17) positive cells could not proliferate well in culture, leading to underestimation of abnormality by G-banding. Monosomy 7 in acute myelocytic leukemia (AML) and myelodysplastic syndrome (MDS) as well as trisomy 12 and deletion chromosome 13 in chronic lymphocytic leukemias (CLL) were also severely underestimated by G-banding. On the other hand, no discrepancies were observed in t(8;21), t(9;22), translations involving 11q23, or in trisomy 8. These findings indicate the superiority of interphase FISH over conventional cytogenetics for detecting chromosome abnormalities in small clones, especially for monosomy 7 or (15;17) translocations.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10565297</pmid><doi>10.1016/S0165-4608(99)00079-5</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-4608 |
| ispartof | Cancer genetics and cytogenetics, 1999-11, Vol.115 (1), p.32-38 |
| issn | 0165-4608 1873-4456 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69281111 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Biological and medical sciences Chromosome Aberrations Chromosome Banding - methods Gene Deletion Humans In Situ Hybridization, Fluorescence - methods Interphase Investigative techniques, diagnostic techniques (general aspects) Leukemia, Lymphocytic, Chronic, B-Cell - genetics Leukemia, Myeloid, Acute - genetics Medical sciences Metaphase Miscellaneous. Technology Monosomy Myelodysplastic Syndromes - genetics Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Translocation, Genetic Trisomy |
| title | Interphase Fluorescence In Situ Hybridization Overcomes Pitfalls of G-Banding Analysis with Special Reference to Underestimation of Chromosomal Aberration Rates |
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