Impact of γ-Radiation on Antigenic Properties of Cow's Milk β-Lactoglobulin
This study evaluated the effects of α-radiation on the antigenic properties of β-lactoglobulin in cow's milk. Liquid and lyophilized samples of cow's milk and whey were irradiated with α-cells (60Co) at dose levels of 3, 5, and 10 kGy, at room temperature in the presence of air. Effects of...
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| Veröffentlicht in: | Journal of food protection 2008-06, Vol.71 (6), p.1270-1272 |
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| creator | Kaddouri, H Mimoun, S El-Mecherfi, K.E Chekroun, A Kheroua, O Saidi, D |
| description | This study evaluated the effects of α-radiation on the antigenic properties of β-lactoglobulin in cow's milk. Liquid and lyophilized samples of cow's milk and whey were irradiated with α-cells (60Co) at dose levels of 3, 5, and 10 kGy, at room temperature in the presence of air. Effects of treatment on proteins were monitored by Lowry's method, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and enzyme-linked immunosorbent assay. Radiation did not affect the molecular-weight distributions of proteins, but it did reduce their solubility. Furthermore, results showed that irradiation at 10 kGy increased the recognition of milk and whey powders by anti-β-lactoglobulin (β-Lg) rabbit immunoglobulin G, with the other samples remaining antigenically stable. These results indicate that α-rays do not reduce cow's milk β-lactoglobulin antigenicity. |
| doi_str_mv | 10.4315/0362-028X-71.6.1270 |
| format | Article |
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Liquid and lyophilized samples of cow's milk and whey were irradiated with α-cells (60Co) at dose levels of 3, 5, and 10 kGy, at room temperature in the presence of air. Effects of treatment on proteins were monitored by Lowry's method, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and enzyme-linked immunosorbent assay. Radiation did not affect the molecular-weight distributions of proteins, but it did reduce their solubility. Furthermore, results showed that irradiation at 10 kGy increased the recognition of milk and whey powders by anti-β-lactoglobulin (β-Lg) rabbit immunoglobulin G, with the other samples remaining antigenically stable. These results indicate that α-rays do not reduce cow's milk β-lactoglobulin antigenicity.</description><identifier>ISSN: 0362-028X</identifier><identifier>EISSN: 1944-9097</identifier><identifier>DOI: 10.4315/0362-028X-71.6.1270</identifier><identifier>PMID: 18592759</identifier><identifier>CODEN: JFPRDR</identifier><language>eng</language><publisher>Des Moines, IA: International Association of Milk, Food and Environmental Sanitarians</publisher><subject>Animals ; antigens ; Biological and medical sciences ; Cattle ; Dose-Response Relationship, Radiation ; Electrophoresis, Polyacrylamide Gel - methods ; Enzyme-Linked Immunosorbent Assay - methods ; food allergies ; Food industries ; Food Irradiation ; food matrix ; Food microbiology ; freeze drying ; Fundamental and applied biological sciences. Psychology ; gamma radiation ; Gamma Rays ; Humans ; immunoglobulin G ; Immunoglobulin G - immunology ; Immunoglobulin G - radiation effects ; lactoglobulins ; Lactoglobulins - immunology ; Lactoglobulins - radiation effects ; milk ; Milk - immunology ; Milk - radiation effects ; Milk and cheese industries. Ice creams ; Milk Hypersensitivity - prevention & control ; polyclonal antibodies ; Solubility ; whey protein</subject><ispartof>Journal of food protection, 2008-06, Vol.71 (6), p.1270-1272</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-3b3e36303e4a693192981c590b768012ce067a4a34ab0700e0a23e7553ce0b923</citedby><cites>FETCH-LOGICAL-c402t-3b3e36303e4a693192981c590b768012ce067a4a34ab0700e0a23e7553ce0b923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20426371$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18592759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaddouri, H</creatorcontrib><creatorcontrib>Mimoun, S</creatorcontrib><creatorcontrib>El-Mecherfi, K.E</creatorcontrib><creatorcontrib>Chekroun, A</creatorcontrib><creatorcontrib>Kheroua, O</creatorcontrib><creatorcontrib>Saidi, D</creatorcontrib><title>Impact of γ-Radiation on Antigenic Properties of Cow's Milk β-Lactoglobulin</title><title>Journal of food protection</title><addtitle>J Food Prot</addtitle><description>This study evaluated the effects of α-radiation on the antigenic properties of β-lactoglobulin in cow's milk. Liquid and lyophilized samples of cow's milk and whey were irradiated with α-cells (60Co) at dose levels of 3, 5, and 10 kGy, at room temperature in the presence of air. Effects of treatment on proteins were monitored by Lowry's method, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and enzyme-linked immunosorbent assay. Radiation did not affect the molecular-weight distributions of proteins, but it did reduce their solubility. Furthermore, results showed that irradiation at 10 kGy increased the recognition of milk and whey powders by anti-β-lactoglobulin (β-Lg) rabbit immunoglobulin G, with the other samples remaining antigenically stable. These results indicate that α-rays do not reduce cow's milk β-lactoglobulin antigenicity.</description><subject>Animals</subject><subject>antigens</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>food allergies</subject><subject>Food industries</subject><subject>Food Irradiation</subject><subject>food matrix</subject><subject>Food microbiology</subject><subject>freeze drying</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma radiation</subject><subject>Gamma Rays</subject><subject>Humans</subject><subject>immunoglobulin G</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulin G - radiation effects</subject><subject>lactoglobulins</subject><subject>Lactoglobulins - immunology</subject><subject>Lactoglobulins - radiation effects</subject><subject>milk</subject><subject>Milk - immunology</subject><subject>Milk - radiation effects</subject><subject>Milk and cheese industries. Ice creams</subject><subject>Milk Hypersensitivity - prevention & control</subject><subject>polyclonal antibodies</subject><subject>Solubility</subject><subject>whey protein</subject><issn>0362-028X</issn><issn>1944-9097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0MtKAzEUBuAgitbqEwg6G3U19eQyyWQpxRtUFC_gLmRipkSnk5pMEV9L38NnMkNLhcBZ5PsPnB-hAwwjRnFxBpSTHEj5kgs84iNMBGygAZaM5RKk2ESDtdhBuzG-AQCRhG-jHVwWkohCDtDtzWyuTZf5Ovv9yR_0q9Od822W3nnbualtncnug5_b0Dkbezf2n6cxu3XNe_b7nU9S2k8bXy0a1-6hrVo30e6v5hA9X148ja_zyd3Vzfh8khsGpMtpRS3lFKhlmkuKJZElNoWESvASMDEWuNBMU6YrEAAWNKFWFAVNP5UkdIhOlnvnwX8sbOzUzEVjm0a31i-i4uk6WRQyQbqEJvgYg63VPLiZDl8Kg-pbVH1Hqu9ICay46ltMqcPV-kU1s6__mVVtCRyvgI5GN3XQrXFx7QgwwqnAyR0tXa290tOQzPMjAUwBJPCSAf0D7JuCsA</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Kaddouri, H</creator><creator>Mimoun, S</creator><creator>El-Mecherfi, K.E</creator><creator>Chekroun, A</creator><creator>Kheroua, O</creator><creator>Saidi, D</creator><general>International Association of Milk, Food and Environmental Sanitarians</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080601</creationdate><title>Impact of γ-Radiation on Antigenic Properties of Cow's Milk β-Lactoglobulin</title><author>Kaddouri, H ; Mimoun, S ; El-Mecherfi, K.E ; Chekroun, A ; Kheroua, O ; Saidi, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-3b3e36303e4a693192981c590b768012ce067a4a34ab0700e0a23e7553ce0b923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>antigens</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>food allergies</topic><topic>Food industries</topic><topic>Food Irradiation</topic><topic>food matrix</topic><topic>Food microbiology</topic><topic>freeze drying</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma radiation</topic><topic>Gamma Rays</topic><topic>Humans</topic><topic>immunoglobulin G</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunoglobulin G - radiation effects</topic><topic>lactoglobulins</topic><topic>Lactoglobulins - immunology</topic><topic>Lactoglobulins - radiation effects</topic><topic>milk</topic><topic>Milk - immunology</topic><topic>Milk - radiation effects</topic><topic>Milk and cheese industries. Ice creams</topic><topic>Milk Hypersensitivity - prevention & control</topic><topic>polyclonal antibodies</topic><topic>Solubility</topic><topic>whey protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaddouri, H</creatorcontrib><creatorcontrib>Mimoun, S</creatorcontrib><creatorcontrib>El-Mecherfi, K.E</creatorcontrib><creatorcontrib>Chekroun, A</creatorcontrib><creatorcontrib>Kheroua, O</creatorcontrib><creatorcontrib>Saidi, D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of food protection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaddouri, H</au><au>Mimoun, S</au><au>El-Mecherfi, K.E</au><au>Chekroun, A</au><au>Kheroua, O</au><au>Saidi, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of γ-Radiation on Antigenic Properties of Cow's Milk β-Lactoglobulin</atitle><jtitle>Journal of food protection</jtitle><addtitle>J Food Prot</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>71</volume><issue>6</issue><spage>1270</spage><epage>1272</epage><pages>1270-1272</pages><issn>0362-028X</issn><eissn>1944-9097</eissn><coden>JFPRDR</coden><abstract>This study evaluated the effects of α-radiation on the antigenic properties of β-lactoglobulin in cow's milk. Liquid and lyophilized samples of cow's milk and whey were irradiated with α-cells (60Co) at dose levels of 3, 5, and 10 kGy, at room temperature in the presence of air. Effects of treatment on proteins were monitored by Lowry's method, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and enzyme-linked immunosorbent assay. Radiation did not affect the molecular-weight distributions of proteins, but it did reduce their solubility. Furthermore, results showed that irradiation at 10 kGy increased the recognition of milk and whey powders by anti-β-lactoglobulin (β-Lg) rabbit immunoglobulin G, with the other samples remaining antigenically stable. These results indicate that α-rays do not reduce cow's milk β-lactoglobulin antigenicity.</abstract><cop>Des Moines, IA</cop><pub>International Association of Milk, Food and Environmental Sanitarians</pub><pmid>18592759</pmid><doi>10.4315/0362-028X-71.6.1270</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals antigens Biological and medical sciences Cattle Dose-Response Relationship, Radiation Electrophoresis, Polyacrylamide Gel - methods Enzyme-Linked Immunosorbent Assay - methods food allergies Food industries Food Irradiation food matrix Food microbiology freeze drying Fundamental and applied biological sciences. Psychology gamma radiation Gamma Rays Humans immunoglobulin G Immunoglobulin G - immunology Immunoglobulin G - radiation effects lactoglobulins Lactoglobulins - immunology Lactoglobulins - radiation effects milk Milk - immunology Milk - radiation effects Milk and cheese industries. Ice creams Milk Hypersensitivity - prevention & control polyclonal antibodies Solubility whey protein |
| title | Impact of γ-Radiation on Antigenic Properties of Cow's Milk β-Lactoglobulin |
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