An Improved Method for the Microscale Preparation and Characterization of Hapten−Protein Conjugates:  The Use of Cholesterol as a Model for Nonchromophore Hydroxylated Haptens

A minute amount (0.446 μmol) of cholesterol (Chol) was converted into an hemisuccinate derivative (Chol HS) using an excess of succinic anhydride. The optimal conditions for synthesis of Chol HS were explored by checkerboard experiments in which various succinic anhydride/Chol molar ratios ranging f...

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