A response regulator‐like protein that functions at an intermediate stage of sporulation in Streptomyces coelicolor A3(2)
whiI is one of several loci originally described as essential for sporulation in Streptomyces coelicolor A3(2). We have characterized whiI at the molecular level. It encodes an atypical member of the response regulator family of proteins, lacking at least two of the residues strongly conserved in th...
Gespeichert in:
| Veröffentlicht in: | Molecular microbiology 1999-11, Vol.34 (3), p.607-619 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 619 |
|---|---|
| container_issue | 3 |
| container_start_page | 607 |
| container_title | Molecular microbiology |
| container_volume | 34 |
| creator | Aínsa, José A. Parry, Huw D. Chater, Keith F. |
| description | whiI is one of several loci originally described as essential for sporulation in Streptomyces coelicolor A3(2). We have characterized whiI at the molecular level. It encodes an atypical member of the response regulator family of proteins, lacking at least two of the residues strongly conserved in the conventional phosphorylation pocket. It is not adjacent to a potential sensor kinase gene. Fifteen mutant alleles of whiI were sequenced, revealing, among others, six mutations affecting conserved amino acids, several frameshift mutations and one mutation in the promoter. The whiI promoter is specifically transcribed by the sporulation‐specific σWhiG‐containing form of RNA polymerase. Transcription of whiI is temporally controlled, reaching a maximum level coincident with the formation of spores. Further transcriptional studies suggested that WhiI is involved directly or indirectly in repressing its own expression and that of another σWhiG‐dependent sporulation‐specific regulatory gene, whiH. |
| doi_str_mv | 10.1046/j.1365-2958.1999.01630.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69273921</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17444749</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4730-bf320a92eca23b514d8d1228d1d67731c414b106bf3263c990275590771527753</originalsourceid><addsrcrecordid>eNqNkc2KFDEQx4Mo7rj6ChI8iB66TeVzcvAwLH4s7OJBBW8hk6lee-zpjEkad_DiI_iMPolpZxHxopekQn71h6ofIRRYC0zqZ9sWhFYNt2rZgrW2ZaAFa69vkcXvj9tkwaxijVjyDyfkXs5bxkAwLe6SE2BKS8VgQb6uaMK8j2PGWlxNgy8x_fj2feg_Id2nWLAfafnoC-2mMZS-grQ-_Ej7sWDa4ab3BWku_gpp7GiNSnNIBStB35aE-xJ3h4CZhohDH-IQE12JJ_zpfXKn80PGBzf3KXn_8sW7s9fNxZtX52eriyZII1iz7gRn3nIMnou1ArlZboDzemy0MQKCBLkGpmdOi2At40Ypy4wBxY1R4pQ8PubWeT5PmIvb9TngMPgR45SdttwIy-GfIBgppZG2go_-ArdxSmMdwoHVqq5Z6Qotj1BIMeeEndunfufTwQFzs0a3dbMtN9tys0b3S6O7rq0Pb_KndV3xH41HbxV4fgS-9AMe_jvYXV6ez5X4CTRtq-Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>196513056</pqid></control><display><type>article</type><title>A response regulator‐like protein that functions at an intermediate stage of sporulation in Streptomyces coelicolor A3(2)</title><source>Electronic Journals Library</source><source>Wiley Online Library website</source><source>MEDLINE</source><source>Wiley Online Library</source><source>Free Full-Text Journals in Chemistry</source><creator>Aínsa, José A. ; Parry, Huw D. ; Chater, Keith F.</creator><creatorcontrib>Aínsa, José A. ; Parry, Huw D. ; Chater, Keith F.</creatorcontrib><description>whiI is one of several loci originally described as essential for sporulation in Streptomyces coelicolor A3(2). We have characterized whiI at the molecular level. It encodes an atypical member of the response regulator family of proteins, lacking at least two of the residues strongly conserved in the conventional phosphorylation pocket. It is not adjacent to a potential sensor kinase gene. Fifteen mutant alleles of whiI were sequenced, revealing, among others, six mutations affecting conserved amino acids, several frameshift mutations and one mutation in the promoter. The whiI promoter is specifically transcribed by the sporulation‐specific σWhiG‐containing form of RNA polymerase. Transcription of whiI is temporally controlled, reaching a maximum level coincident with the formation of spores. Further transcriptional studies suggested that WhiI is involved directly or indirectly in repressing its own expression and that of another σWhiG‐dependent sporulation‐specific regulatory gene, whiH.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.1999.01630.x</identifier><identifier>PMID: 10564501</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>Amino Acid Sequence ; Bacterial Proteins ; Base Sequence ; Cloning, Molecular ; frameshift mutant ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Genetic Complementation Test ; Molecular Sequence Data ; Mutation ; Promoter Regions, Genetic ; Repressor Proteins - chemistry ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Repressor Proteins - physiology ; Sequence Alignment ; Sequence Analysis, DNA ; Spores, Bacterial - genetics ; Spores, Bacterial - physiology ; Streptomyces - genetics ; Streptomyces - physiology ; Streptomyces coelicolor ; Whil gene</subject><ispartof>Molecular microbiology, 1999-11, Vol.34 (3), p.607-619</ispartof><rights>Copyright Blackwell Scientific Publications Ltd. Nov 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4730-bf320a92eca23b514d8d1228d1d67731c414b106bf3263c990275590771527753</citedby><cites>FETCH-LOGICAL-c4730-bf320a92eca23b514d8d1228d1d67731c414b106bf3263c990275590771527753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2958.1999.01630.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2958.1999.01630.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10564501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aínsa, José A.</creatorcontrib><creatorcontrib>Parry, Huw D.</creatorcontrib><creatorcontrib>Chater, Keith F.</creatorcontrib><title>A response regulator‐like protein that functions at an intermediate stage of sporulation in Streptomyces coelicolor A3(2)</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>whiI is one of several loci originally described as essential for sporulation in Streptomyces coelicolor A3(2). We have characterized whiI at the molecular level. It encodes an atypical member of the response regulator family of proteins, lacking at least two of the residues strongly conserved in the conventional phosphorylation pocket. It is not adjacent to a potential sensor kinase gene. Fifteen mutant alleles of whiI were sequenced, revealing, among others, six mutations affecting conserved amino acids, several frameshift mutations and one mutation in the promoter. The whiI promoter is specifically transcribed by the sporulation‐specific σWhiG‐containing form of RNA polymerase. Transcription of whiI is temporally controlled, reaching a maximum level coincident with the formation of spores. Further transcriptional studies suggested that WhiI is involved directly or indirectly in repressing its own expression and that of another σWhiG‐dependent sporulation‐specific regulatory gene, whiH.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>frameshift mutant</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>Genetic Complementation Test</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Promoter Regions, Genetic</subject><subject>Repressor Proteins - chemistry</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Repressor Proteins - physiology</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Spores, Bacterial - genetics</subject><subject>Spores, Bacterial - physiology</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - physiology</subject><subject>Streptomyces coelicolor</subject><subject>Whil gene</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2KFDEQx4Mo7rj6ChI8iB66TeVzcvAwLH4s7OJBBW8hk6lee-zpjEkad_DiI_iMPolpZxHxopekQn71h6ofIRRYC0zqZ9sWhFYNt2rZgrW2ZaAFa69vkcXvj9tkwaxijVjyDyfkXs5bxkAwLe6SE2BKS8VgQb6uaMK8j2PGWlxNgy8x_fj2feg_Id2nWLAfafnoC-2mMZS-grQ-_Ej7sWDa4ab3BWku_gpp7GiNSnNIBStB35aE-xJ3h4CZhohDH-IQE12JJ_zpfXKn80PGBzf3KXn_8sW7s9fNxZtX52eriyZII1iz7gRn3nIMnou1ArlZboDzemy0MQKCBLkGpmdOi2At40Ypy4wBxY1R4pQ8PubWeT5PmIvb9TngMPgR45SdttwIy-GfIBgppZG2go_-ArdxSmMdwoHVqq5Z6Qotj1BIMeeEndunfufTwQFzs0a3dbMtN9tys0b3S6O7rq0Pb_KndV3xH41HbxV4fgS-9AMe_jvYXV6ez5X4CTRtq-Q</recordid><startdate>199911</startdate><enddate>199911</enddate><creator>Aínsa, José A.</creator><creator>Parry, Huw D.</creator><creator>Chater, Keith F.</creator><general>Blackwell Science Ltd</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199911</creationdate><title>A response regulator‐like protein that functions at an intermediate stage of sporulation in Streptomyces coelicolor A3(2)</title><author>Aínsa, José A. ; Parry, Huw D. ; Chater, Keith F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4730-bf320a92eca23b514d8d1228d1d67731c414b106bf3263c990275590771527753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>frameshift mutant</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial</topic><topic>Genetic Complementation Test</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Promoter Regions, Genetic</topic><topic>Repressor Proteins - chemistry</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Repressor Proteins - physiology</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Spores, Bacterial - genetics</topic><topic>Spores, Bacterial - physiology</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - physiology</topic><topic>Streptomyces coelicolor</topic><topic>Whil gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aínsa, José A.</creatorcontrib><creatorcontrib>Parry, Huw D.</creatorcontrib><creatorcontrib>Chater, Keith F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aínsa, José A.</au><au>Parry, Huw D.</au><au>Chater, Keith F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A response regulator‐like protein that functions at an intermediate stage of sporulation in Streptomyces coelicolor A3(2)</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1999-11</date><risdate>1999</risdate><volume>34</volume><issue>3</issue><spage>607</spage><epage>619</epage><pages>607-619</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>whiI is one of several loci originally described as essential for sporulation in Streptomyces coelicolor A3(2). We have characterized whiI at the molecular level. It encodes an atypical member of the response regulator family of proteins, lacking at least two of the residues strongly conserved in the conventional phosphorylation pocket. It is not adjacent to a potential sensor kinase gene. Fifteen mutant alleles of whiI were sequenced, revealing, among others, six mutations affecting conserved amino acids, several frameshift mutations and one mutation in the promoter. The whiI promoter is specifically transcribed by the sporulation‐specific σWhiG‐containing form of RNA polymerase. Transcription of whiI is temporally controlled, reaching a maximum level coincident with the formation of spores. Further transcriptional studies suggested that WhiI is involved directly or indirectly in repressing its own expression and that of another σWhiG‐dependent sporulation‐specific regulatory gene, whiH.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>10564501</pmid><doi>10.1046/j.1365-2958.1999.01630.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-382X |
| ispartof | Molecular microbiology, 1999-11, Vol.34 (3), p.607-619 |
| issn | 0950-382X 1365-2958 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69273921 |
| source | Electronic Journals Library; Wiley Online Library website; MEDLINE; Wiley Online Library; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Bacterial Proteins Base Sequence Cloning, Molecular frameshift mutant Gene Expression Regulation, Bacterial Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Mutation Promoter Regions, Genetic Repressor Proteins - chemistry Repressor Proteins - genetics Repressor Proteins - metabolism Repressor Proteins - physiology Sequence Alignment Sequence Analysis, DNA Spores, Bacterial - genetics Spores, Bacterial - physiology Streptomyces - genetics Streptomyces - physiology Streptomyces coelicolor Whil gene |
| title | A response regulator‐like protein that functions at an intermediate stage of sporulation in Streptomyces coelicolor A3(2) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A05%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20response%20regulator%E2%80%90like%20protein%20that%20functions%20at%20an%20intermediate%20stage%20of%20sporulation%20in%20Streptomyces%20coelicolor%20A3(2)&rft.jtitle=Molecular%20microbiology&rft.au=A%C3%ADnsa,%20Jos%C3%A9%20A.&rft.date=1999-11&rft.volume=34&rft.issue=3&rft.spage=607&rft.epage=619&rft.pages=607-619&rft.issn=0950-382X&rft.eissn=1365-2958&rft_id=info:doi/10.1046/j.1365-2958.1999.01630.x&rft_dat=%3Cproquest_cross%3E17444749%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=196513056&rft_id=info:pmid/10564501&rfr_iscdi=true |