Determination of the Wax Ester Content in Olive Oils. Improvement in the Method Proposed by EEC Regulation 183/93

A simpler and faster procedure than the official one described in document IV of European Economic Community Regulation 183/93 is proposed. The wax ester fraction is isolated from triglycerides using a commercially available silica gel column and carbon tetrachloride as eluent. The recovered wax est...

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Veröffentlicht in:	Journal of agricultural and food chemistry 1999-01, Vol.47 (1), p.202-205
Hauptverfasser:	Nota, Giorgio, Naviglio, Daniele, Romano, Raffaele, Sabia, Valentina, Musso, Salvatore Spagna, Improta, Carlo
Format:	Artikel
Sprache:	eng
Schlagworte:	analytical methods Biological and medical sciences Chromatography, Gas determination Esters eu regulations Fat industries food composition Food industries Fundamental and applied biological sciences. Psychology Olive Oil Plant Oils - chemistry wax esters
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A simpler and faster procedure than the official one described in document IV of European Economic Community Regulation 183/93 is proposed. The wax ester fraction is isolated from triglycerides using a commercially available silica gel column and carbon tetrachloride as eluent. The recovered wax ester fraction, with the addition of a suitable internal standard solution, is analyzed by gas chromatography. A column with a 65% phenyl methyl silicone stationary phase allows a satisfying separation of wax ester fraction in comparison with both a steryl ester and a light fraction eluted before the internal standard. Furthermore, also the single components of the wax ester fraction are suitably separated. Keywords: GC; olive oil; solid-phase extraction; steryl esters; wax esters
ISSN:	0021-8561 1520-5118
DOI:	10.1021/jf980648j