Vasoactive intestinal peptide stimulates apical secretion in hamster seminal vesicle epithelial cells in culture
In this study, we investigated the presence of vasoactive intestinal peptide (VIP) receptors in the epithelial cells of the hamster seminal vesicle, by using cell clusters isolated from the gland and cultivated in a serum-free bicameral culture system. An immunocytochemical approach and autoradiogra...
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Veröffentlicht in: | Cell and tissue research 1999-10, Vol.298 (1), p.137-143 |
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creator | Rodrigues, G Cachaço, A S Gulbenkian, S Mata, L |
description | In this study, we investigated the presence of vasoactive intestinal peptide (VIP) receptors in the epithelial cells of the hamster seminal vesicle, by using cell clusters isolated from the gland and cultivated in a serum-free bicameral culture system. An immunocytochemical approach and autoradiographic and biochemical binding experiments with 125I-VIP as radioligand were performed. The effect of this neuropeptide on cultured cell proliferation and protein secretory activity was also analysed. The release of trichloroacetic-acid-precipitable radioactive material by epithelial cells to the apical and basal compartments of the bi-chamber was estimated in absolute and relative terms. The results of this work indicate that: (1) VIP receptors are present in the membranes of clusters of epithelial cells from seminal vesicles and are further maintained in cultured cells; (2) VIP does not exert any mitogenic effect in these cells; (3) VIP affects the directionality of secretion, favouring the absolute and relative amounts of protein released to the apical compartment of the bi-chamber. The expression of VIP receptors in the epithelial cells of the hamster seminal vesicle and the direct secretagogue-like activity of this neuropeptide in these cells might be affected by other factors, namely, androgens. |
doi_str_mv | 10.1007/s004419900070 |
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An immunocytochemical approach and autoradiographic and biochemical binding experiments with 125I-VIP as radioligand were performed. The effect of this neuropeptide on cultured cell proliferation and protein secretory activity was also analysed. The release of trichloroacetic-acid-precipitable radioactive material by epithelial cells to the apical and basal compartments of the bi-chamber was estimated in absolute and relative terms. The results of this work indicate that: (1) VIP receptors are present in the membranes of clusters of epithelial cells from seminal vesicles and are further maintained in cultured cells; (2) VIP does not exert any mitogenic effect in these cells; (3) VIP affects the directionality of secretion, favouring the absolute and relative amounts of protein released to the apical compartment of the bi-chamber. The expression of VIP receptors in the epithelial cells of the hamster seminal vesicle and the direct secretagogue-like activity of this neuropeptide in these cells might be affected by other factors, namely, androgens.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s004419900070</identifier><identifier>PMID: 10555547</identifier><language>eng</language><publisher>Germany</publisher><subject>Animals ; Autoradiography ; Cell Division - drug effects ; Cells, Cultured ; Cricetinae ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Immunohistochemistry ; Male ; Proteins - metabolism ; Radioligand Assay ; Receptors, Vasoactive Intestinal Peptide - metabolism ; Seminal Vesicles - drug effects ; Seminal Vesicles - metabolism ; Vasoactive Intestinal Peptide - metabolism ; Vasoactive Intestinal Peptide - pharmacology</subject><ispartof>Cell and tissue research, 1999-10, Vol.298 (1), p.137-143</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10555547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodrigues, G</creatorcontrib><creatorcontrib>Cachaço, A S</creatorcontrib><creatorcontrib>Gulbenkian, S</creatorcontrib><creatorcontrib>Mata, L</creatorcontrib><title>Vasoactive intestinal peptide stimulates apical secretion in hamster seminal vesicle epithelial cells in culture</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><description>In this study, we investigated the presence of vasoactive intestinal peptide (VIP) receptors in the epithelial cells of the hamster seminal vesicle, by using cell clusters isolated from the gland and cultivated in a serum-free bicameral culture system. An immunocytochemical approach and autoradiographic and biochemical binding experiments with 125I-VIP as radioligand were performed. The effect of this neuropeptide on cultured cell proliferation and protein secretory activity was also analysed. The release of trichloroacetic-acid-precipitable radioactive material by epithelial cells to the apical and basal compartments of the bi-chamber was estimated in absolute and relative terms. The results of this work indicate that: (1) VIP receptors are present in the membranes of clusters of epithelial cells from seminal vesicles and are further maintained in cultured cells; (2) VIP does not exert any mitogenic effect in these cells; (3) VIP affects the directionality of secretion, favouring the absolute and relative amounts of protein released to the apical compartment of the bi-chamber. The expression of VIP receptors in the epithelial cells of the hamster seminal vesicle and the direct secretagogue-like activity of this neuropeptide in these cells might be affected by other factors, namely, androgens.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>Cricetinae</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Proteins - metabolism</subject><subject>Radioligand Assay</subject><subject>Receptors, Vasoactive Intestinal Peptide - metabolism</subject><subject>Seminal Vesicles - drug effects</subject><subject>Seminal Vesicles - metabolism</subject><subject>Vasoactive Intestinal Peptide - metabolism</subject><subject>Vasoactive Intestinal Peptide - pharmacology</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1PwzAMhiMEYmNw5Ip64lZw0iRtj2jiS5rEBRC3Kk0dLShdS5JO4t-TsR3AF8uvH1v2S8glhRsKUN4GAM5pXUMq4IjMKS9YDlVZHZM5FMDyUsqPGTkL4ROAcinrUzKjIFLwck7GdxUGpaPdYmY3EUO0G-WyEcdoO8xS2U9OJT1To9WpE1B7jHbYJDxbqz5E9Ensf8e2GKx2mOFo4xqdTZJG58KO1ZOLk8dzcmKUC3hxyAvy9nD_unzKVy-Pz8u7Va4ZFzHvGG0ZMKEMLaFVhRSKV0YwWQmKDI1BYFhWXLZCVIbLQpeso6rFlneMd6ZYkOv93tEPX1P6q-lt2B2jNjhMoZE1kwJokcB8D2o_hODRNKO3vfLfDYVmZ3Hzz-LEXx0WT22P3R9672nxA97heRw</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Rodrigues, G</creator><creator>Cachaço, A S</creator><creator>Gulbenkian, S</creator><creator>Mata, L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Vasoactive intestinal peptide stimulates apical secretion in hamster seminal vesicle epithelial cells in culture</title><author>Rodrigues, G ; Cachaço, A S ; Gulbenkian, S ; Mata, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c245t-d21b2025af170ba365a48f526851e2effe02e7846b558f463c72d1abeb4d24df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>Cricetinae</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Proteins - metabolism</topic><topic>Radioligand Assay</topic><topic>Receptors, Vasoactive Intestinal Peptide - metabolism</topic><topic>Seminal Vesicles - drug effects</topic><topic>Seminal Vesicles - metabolism</topic><topic>Vasoactive Intestinal Peptide - metabolism</topic><topic>Vasoactive Intestinal Peptide - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodrigues, G</creatorcontrib><creatorcontrib>Cachaço, A S</creatorcontrib><creatorcontrib>Gulbenkian, S</creatorcontrib><creatorcontrib>Mata, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodrigues, G</au><au>Cachaço, A S</au><au>Gulbenkian, S</au><au>Mata, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vasoactive intestinal peptide stimulates apical secretion in hamster seminal vesicle epithelial cells in culture</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>298</volume><issue>1</issue><spage>137</spage><epage>143</epage><pages>137-143</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>In this study, we investigated the presence of vasoactive intestinal peptide (VIP) receptors in the epithelial cells of the hamster seminal vesicle, by using cell clusters isolated from the gland and cultivated in a serum-free bicameral culture system. An immunocytochemical approach and autoradiographic and biochemical binding experiments with 125I-VIP as radioligand were performed. The effect of this neuropeptide on cultured cell proliferation and protein secretory activity was also analysed. The release of trichloroacetic-acid-precipitable radioactive material by epithelial cells to the apical and basal compartments of the bi-chamber was estimated in absolute and relative terms. The results of this work indicate that: (1) VIP receptors are present in the membranes of clusters of epithelial cells from seminal vesicles and are further maintained in cultured cells; (2) VIP does not exert any mitogenic effect in these cells; (3) VIP affects the directionality of secretion, favouring the absolute and relative amounts of protein released to the apical compartment of the bi-chamber. The expression of VIP receptors in the epithelial cells of the hamster seminal vesicle and the direct secretagogue-like activity of this neuropeptide in these cells might be affected by other factors, namely, androgens.</abstract><cop>Germany</cop><pmid>10555547</pmid><doi>10.1007/s004419900070</doi><tpages>7</tpages></addata></record> |
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subjects | Animals Autoradiography Cell Division - drug effects Cells, Cultured Cricetinae Epithelial Cells - drug effects Epithelial Cells - metabolism Immunohistochemistry Male Proteins - metabolism Radioligand Assay Receptors, Vasoactive Intestinal Peptide - metabolism Seminal Vesicles - drug effects Seminal Vesicles - metabolism Vasoactive Intestinal Peptide - metabolism Vasoactive Intestinal Peptide - pharmacology |
title | Vasoactive intestinal peptide stimulates apical secretion in hamster seminal vesicle epithelial cells in culture |
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