Seroprevalence of anti-Borrelia burgdorferi antibodies in dogs and horses in Turkey
The aim of the study was to determine the seroprevalence of anti-Borrelia burgdorferi antibodies in a population of Turkish dogs and horses, as well as to compare the sensitivity of novel flow-cytometry-based borreliacidal antibody test (BAT) with ELISA assay. Serum samples collected from 400 dogs a...
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| Veröffentlicht in: | Annals of Agricultural and Environmental Medicine 2008, Vol.15 (1), p.85-90 |
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| creator | Bhide, Mangesh Yilmaz, Zeki Golcu, Esin Torun, Serhat Mikula, Ivan |
| description | The aim of the study was to determine the seroprevalence of anti-Borrelia burgdorferi antibodies in a population of Turkish dogs and horses, as well as to compare the sensitivity of novel flow-cytometry-based borreliacidal antibody test (BAT) with ELISA assay. Serum samples collected from 400 dogs and 300 horses were tested with enzyme-linked protein A/G assay (ELPAGA), using Borrelia whole cell antigens. ELPAGA test showed 93 dogs (23.2%) and 18 horses (6%) serologically positive for anti-Borrelia antibodies. In parallel testing of sera with BAT, we found 27.75% positive dogs and 6.33% positive horses. When the results of these serological testes were compared with the health status of the animals, the most common clinical signs noticed in dogs were skin manifestations, urinary tract disorder and anemia; however, no clinical symptoms were observed in horses positive for the anti-Borrelia antibodies. This is a first time that seroprevalence of Lyme disease in dogs and horses has been reported from Turkey, as well as the use of novel BAT in animals. |
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Serum samples collected from 400 dogs and 300 horses were tested with enzyme-linked protein A/G assay (ELPAGA), using Borrelia whole cell antigens. ELPAGA test showed 93 dogs (23.2%) and 18 horses (6%) serologically positive for anti-Borrelia antibodies. In parallel testing of sera with BAT, we found 27.75% positive dogs and 6.33% positive horses. When the results of these serological testes were compared with the health status of the animals, the most common clinical signs noticed in dogs were skin manifestations, urinary tract disorder and anemia; however, no clinical symptoms were observed in horses positive for the anti-Borrelia antibodies. This is a first time that seroprevalence of Lyme disease in dogs and horses has been reported from Turkey, as well as the use of novel BAT in animals.</description><identifier>ISSN: 1232-1966</identifier><identifier>EISSN: 1898-2263</identifier><identifier>PMID: 18581984</identifier><language>eng</language><publisher>Poland: Institute of Rural Health</publisher><subject>Animals ; Antibodies ; Antibodies, Bacterial - blood ; Antigens ; Borrelia ; Borrelia burgdorferi ; Borrelia burgdorferi - immunology ; Cytometry ; Dog Diseases - epidemiology ; Dogs ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - standards ; Enzyme-Linked Immunosorbent Assay - veterinary ; Female ; Flow Cytometry - methods ; Flow Cytometry - standards ; Flow Cytometry - veterinary ; Horse Diseases - epidemiology ; Horses ; Lyme disease ; Lyme Disease - epidemiology ; Male ; Sensitivity and Specificity ; Seroepidemiologic Studies ; Serology ; Turkey - epidemiology ; Urinary tract ; Vector-borne diseases</subject><ispartof>Annals of Agricultural and Environmental Medicine, 2008, Vol.15 (1), p.85-90</ispartof><rights>2008. 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Serum samples collected from 400 dogs and 300 horses were tested with enzyme-linked protein A/G assay (ELPAGA), using Borrelia whole cell antigens. ELPAGA test showed 93 dogs (23.2%) and 18 horses (6%) serologically positive for anti-Borrelia antibodies. In parallel testing of sera with BAT, we found 27.75% positive dogs and 6.33% positive horses. When the results of these serological testes were compared with the health status of the animals, the most common clinical signs noticed in dogs were skin manifestations, urinary tract disorder and anemia; however, no clinical symptoms were observed in horses positive for the anti-Borrelia antibodies. 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Yilmaz, Zeki ; Golcu, Esin ; Torun, Serhat ; Mikula, Ivan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p268t-e392c0962304dcdc1c61d16a99a4080409f9a5aad4770e2b36f4ec4a42d15a233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antigens</topic><topic>Borrelia</topic><topic>Borrelia burgdorferi</topic><topic>Borrelia burgdorferi - immunology</topic><topic>Cytometry</topic><topic>Dog Diseases - epidemiology</topic><topic>Dogs</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - standards</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Female</topic><topic>Flow Cytometry - methods</topic><topic>Flow Cytometry - standards</topic><topic>Flow Cytometry - veterinary</topic><topic>Horse Diseases - epidemiology</topic><topic>Horses</topic><topic>Lyme disease</topic><topic>Lyme Disease - epidemiology</topic><topic>Male</topic><topic>Sensitivity and Specificity</topic><topic>Seroepidemiologic Studies</topic><topic>Serology</topic><topic>Turkey - epidemiology</topic><topic>Urinary tract</topic><topic>Vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhide, Mangesh</creatorcontrib><creatorcontrib>Yilmaz, Zeki</creatorcontrib><creatorcontrib>Golcu, Esin</creatorcontrib><creatorcontrib>Torun, Serhat</creatorcontrib><creatorcontrib>Mikula, Ivan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of Agricultural and Environmental Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhide, Mangesh</au><au>Yilmaz, Zeki</au><au>Golcu, Esin</au><au>Torun, Serhat</au><au>Mikula, Ivan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seroprevalence of anti-Borrelia burgdorferi antibodies in dogs and horses in Turkey</atitle><jtitle>Annals of Agricultural and Environmental Medicine</jtitle><addtitle>Ann Agric Environ Med</addtitle><date>2008</date><risdate>2008</risdate><volume>15</volume><issue>1</issue><spage>85</spage><epage>90</epage><pages>85-90</pages><issn>1232-1966</issn><eissn>1898-2263</eissn><abstract>The aim of the study was to determine the seroprevalence of anti-Borrelia burgdorferi antibodies in a population of Turkish dogs and horses, as well as to compare the sensitivity of novel flow-cytometry-based borreliacidal antibody test (BAT) with ELISA assay. 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| subjects | Animals Antibodies Antibodies, Bacterial - blood Antigens Borrelia Borrelia burgdorferi Borrelia burgdorferi - immunology Cytometry Dog Diseases - epidemiology Dogs Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - standards Enzyme-Linked Immunosorbent Assay - veterinary Female Flow Cytometry - methods Flow Cytometry - standards Flow Cytometry - veterinary Horse Diseases - epidemiology Horses Lyme disease Lyme Disease - epidemiology Male Sensitivity and Specificity Seroepidemiologic Studies Serology Turkey - epidemiology Urinary tract Vector-borne diseases |
| title | Seroprevalence of anti-Borrelia burgdorferi antibodies in dogs and horses in Turkey |
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