Comparing the extent of protein build-up on several disposable lenses by two spectrophotometric methods
This study aims to quantify levels of protein build-up on high-water ionic (Type IV) disposable contact lenses worn by patients, by two spectrophotometric methods, previously validated only with conventional lenses. Forty lenses were analyzed and the related results were compared. The Ninhydrine-Lys...
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| Veröffentlicht in: | Contact lens & anterior eye 1998, Vol.21 (4), p.104-108 |
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| container_title | Contact lens & anterior eye |
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| creator | Michaud, Langis Giasson, Claude |
| description | This study aims to quantify levels of protein build-up on high-water ionic (Type IV) disposable contact lenses worn by patients, by two spectrophotometric methods, previously validated only with conventional lenses. Forty lenses were analyzed and the related results were compared. The Ninhydrine-Lysozyme method and the Coomassie Blue Albumin one agreed in identifying the most spoiled lenses. Etafilcon A attracted significantly more protein than the other materials tested, which were in the following decreasing order of protein spoilation: Ocuflcon D, Ocufilcon A and Vasurfilcon A. These similar findings suggest that it is possible to use the above mentioned analysis methods for an accurate evaluation of the contamination of several materials of disposable/frequent replacement lenses as for conventional lenses. |
| doi_str_mv | 10.1016/S1367-0484(98)80003-9 |
| format | Article |
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Forty lenses were analyzed and the related results were compared. The Ninhydrine-Lysozyme method and the Coomassie Blue Albumin one agreed in identifying the most spoiled lenses. Etafilcon A attracted significantly more protein than the other materials tested, which were in the following decreasing order of protein spoilation: Ocuflcon D, Ocufilcon A and Vasurfilcon A. 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Forty lenses were analyzed and the related results were compared. The Ninhydrine-Lysozyme method and the Coomassie Blue Albumin one agreed in identifying the most spoiled lenses. Etafilcon A attracted significantly more protein than the other materials tested, which were in the following decreasing order of protein spoilation: Ocuflcon D, Ocufilcon A and Vasurfilcon A. These similar findings suggest that it is possible to use the above mentioned analysis methods for an accurate evaluation of the contamination of several materials of disposable/frequent replacement lenses as for conventional lenses.</description><subject>chemical assays</subject><subject>Coomassie Blue analysis method</subject><subject>disposable contact lenses</subject><subject>frequent replacement contact lenses</subject><subject>hydrophilic contact lenses</subject><subject>ninhydrine analysis method</subject><subject>protein deposits</subject><subject>spectrophotometric measures</subject><issn>1367-0484</issn><issn>1476-5411</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkE1P7SAQhom5xu-f4A2rG11UobRQVsac-JWYuFDXpMDUg2lLL1A__r3oOcalqyGZ550ZHoQOKTmhhPLTe8q4KEjVVEeyOW4IIayQG2iHVoIXdUXpn_z-RrbRbozPJOekJFtom3JGGGvEDnpa-GFqgxufcFoChrcEY8K-w1PwCdyI9ex6W8wT9iOO8AKh7bF1cfKx1T3gHsYIEet3nF49jhOYFPy09MkPkIIzOJelt3EfbXZtH-FgXffQ4-XFw-K6uL27ulmc3xamFDwVghIwjQVea8sNaw0XbcmJAWMqXglK6zr3ZdeIkpFS1w2tOgui5EA000azPfRvNTff_3-GmNTgooG-b0fwc1RclrUUnGewXoEm-BgDdGoKbmjDu6JEfRpWX4bVpz4lG_VlWMmc-7teMOsB7E9qrTQDZysA8jdfHAQVjYPRgHUh21HWu19WfACu6I10</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Michaud, Langis</creator><creator>Giasson, Claude</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>Comparing the extent of protein build-up on several disposable lenses by two spectrophotometric methods</title><author>Michaud, Langis ; Giasson, Claude</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c276t-710ec8de65bd6c3ac67a260cecc46471155ec89f872302b5814fde726e0b3bcb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>chemical assays</topic><topic>Coomassie Blue analysis method</topic><topic>disposable contact lenses</topic><topic>frequent replacement contact lenses</topic><topic>hydrophilic contact lenses</topic><topic>ninhydrine analysis method</topic><topic>protein deposits</topic><topic>spectrophotometric measures</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Michaud, Langis</creatorcontrib><creatorcontrib>Giasson, Claude</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Contact lens & anterior eye</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Michaud, Langis</au><au>Giasson, Claude</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparing the extent of protein build-up on several disposable lenses by two spectrophotometric methods</atitle><jtitle>Contact lens & anterior eye</jtitle><addtitle>Cont Lens Anterior Eye</addtitle><date>1998</date><risdate>1998</risdate><volume>21</volume><issue>4</issue><spage>104</spage><epage>108</epage><pages>104-108</pages><issn>1367-0484</issn><eissn>1476-5411</eissn><abstract>This study aims to quantify levels of protein build-up on high-water ionic (Type IV) disposable contact lenses worn by patients, by two spectrophotometric methods, previously validated only with conventional lenses. 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| ispartof | Contact lens & anterior eye, 1998, Vol.21 (4), p.104-108 |
| issn | 1367-0484 1476-5411 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | chemical assays Coomassie Blue analysis method disposable contact lenses frequent replacement contact lenses hydrophilic contact lenses ninhydrine analysis method protein deposits spectrophotometric measures |
| title | Comparing the extent of protein build-up on several disposable lenses by two spectrophotometric methods |
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