Sequencing of 42kb of the APO E-C2 Gene Cluster Reveals a New Gene: PEREC1
Through the sequencing of a 42kb cosmid clone we describe a new gene, designated PEREC1, located approximately 1.5kb centromeric of the human apolipoprotein (APO) E-C2 cluster. The combination of dotplot analysis, predicted coding potential and interrogation of the Expressed Sequence Tag (EST) datab...
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| Veröffentlicht in: | DNA sequence 1998, Vol.9 (2), p.89-100 |
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| creator | Freitas, Elizabeth M Zhang, Wen Jie Lalonde, Jean-Philippe Tay, Guan K. Gaudieri, Silvana Ashworth, Linda K Dawkins, Roger L |
| description | Through the sequencing of a 42kb cosmid clone we describe a new gene, designated PEREC1, located approximately 1.5kb centromeric of the human apolipoprotein (APO) E-C2 cluster. The combination of dotplot analysis, predicted coding potential and interrogation of the Expressed Sequence Tag (EST) database determined the genomic organisation of PEREC1. Sequence alignment with multiple overlapping ESTs confirmed the predicted splice sites. The predicted cDNA and amino acid sequences of PEREC1 have extensive similarity to the Caenorhabditis elegans protein, C18E9.6. Conserved structural and functional motifs have been defined by combining nucleotide and amino acid analyses to identify third base degeneracy and therefore selection at the protein level.
The Poliovirus Receptor Related Protein2 gene (PRR2), previously mapped to chromosome 19ql3.2 by Fluorescent In-Situ Hybridisation, has also been located approximately 17kb centromeric of APO E. |
| doi_str_mv | 10.3109/10425179809086433 |
| format | Article |
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The Poliovirus Receptor Related Protein2 gene (PRR2), previously mapped to chromosome 19ql3.2 by Fluorescent In-Situ Hybridisation, has also been located approximately 17kb centromeric of APO E.</description><identifier>ISSN: 1042-5179</identifier><identifier>EISSN: 1029-2365</identifier><identifier>DOI: 10.3109/10425179809086433</identifier><identifier>PMID: 10520737</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Alzheimer Disease - genetics ; Amino Acid Motifs ; Amino Acid Sequence ; Animals ; Apolipoproteins E - chemistry ; Apolipoproteins E - genetics ; Base Sequence ; Caenorhabditis elegans - genetics ; Cell Adhesion Molecules ; Chromosome Mapping ; Chromosomes, Human, Pair 19 - genetics ; Coronary Disease - genetics ; Cosmids - genetics ; Databases, Factual ; Expressed Sequence Tags ; Helminth Proteins - chemistry ; Helminth Proteins - genetics ; Humans ; Membrane Glycoproteins - chemistry ; Membrane Glycoproteins - genetics ; Membrane Transport Proteins ; Molecular Sequence Data ; Multigene Family ; Nectins ; Proteins - chemistry ; Proteins - genetics ; Proteins - metabolism ; Receptors, Tumor Necrosis Factor ; Receptors, Tumor Necrosis Factor, Member 14 ; Receptors, Virus ; Sequence Analysis, DNA ; Sequence Analysis, Protein</subject><ispartof>DNA sequence, 1998, Vol.9 (2), p.89-100</ispartof><rights>1998 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1998</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-131c309508dbdeafc013d303af8a1bc1bf93e4b584e2f1ed70eda4a4404702f83</citedby><cites>FETCH-LOGICAL-c402t-131c309508dbdeafc013d303af8a1bc1bf93e4b584e2f1ed70eda4a4404702f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/10425179809086433$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/10425179809086433$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,60436,61221,61402</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10520737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Freitas, Elizabeth M</creatorcontrib><creatorcontrib>Zhang, Wen Jie</creatorcontrib><creatorcontrib>Lalonde, Jean-Philippe</creatorcontrib><creatorcontrib>Tay, Guan K.</creatorcontrib><creatorcontrib>Gaudieri, Silvana</creatorcontrib><creatorcontrib>Ashworth, Linda K</creatorcontrib><creatorcontrib>Dawkins, Roger L</creatorcontrib><title>Sequencing of 42kb of the APO E-C2 Gene Cluster Reveals a New Gene: PEREC1</title><title>DNA sequence</title><addtitle>DNA Seq</addtitle><description>Through the sequencing of a 42kb cosmid clone we describe a new gene, designated PEREC1, located approximately 1.5kb centromeric of the human apolipoprotein (APO) E-C2 cluster. The combination of dotplot analysis, predicted coding potential and interrogation of the Expressed Sequence Tag (EST) database determined the genomic organisation of PEREC1. Sequence alignment with multiple overlapping ESTs confirmed the predicted splice sites. The predicted cDNA and amino acid sequences of PEREC1 have extensive similarity to the Caenorhabditis elegans protein, C18E9.6. Conserved structural and functional motifs have been defined by combining nucleotide and amino acid analyses to identify third base degeneracy and therefore selection at the protein level.
The Poliovirus Receptor Related Protein2 gene (PRR2), previously mapped to chromosome 19ql3.2 by Fluorescent In-Situ Hybridisation, has also been located approximately 17kb centromeric of APO E.</description><subject>Alzheimer Disease - genetics</subject><subject>Amino Acid Motifs</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Apolipoproteins E - chemistry</subject><subject>Apolipoproteins E - genetics</subject><subject>Base Sequence</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Cell Adhesion Molecules</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 19 - genetics</subject><subject>Coronary Disease - genetics</subject><subject>Cosmids - genetics</subject><subject>Databases, Factual</subject><subject>Expressed Sequence Tags</subject><subject>Helminth Proteins - chemistry</subject><subject>Helminth Proteins - genetics</subject><subject>Humans</subject><subject>Membrane Glycoproteins - chemistry</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Transport Proteins</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Nectins</subject><subject>Proteins - chemistry</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Receptors, Tumor Necrosis Factor</subject><subject>Receptors, Tumor Necrosis Factor, Member 14</subject><subject>Receptors, Virus</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Analysis, Protein</subject><issn>1042-5179</issn><issn>1029-2365</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PwkAQhjdGI4j-AC9mT96qsx-lrXohTUUNEYJ6brbtrIClxd1Wwr-3FQ4ao6eZZJ73zeQh5JTBhWAQXDKQ3GVe4EMAfl8KsUe6DHjgcNF399tdcqcFOuTI2gUAuK7vH5IOA5eDJ7wueXjC9xqLdF680lJTyd-SdlYzpIPJmEZOyOkQC6RhXtsKDZ3iB6rcUkUfcf11uqKTaBqF7Jgc6OaCJ7vZIy-30XN454zGw_twMHJSCbxymGCpgMAFP0syVDoFJjIBQmlfsSRliQ4EysT1JXLNMPMAMyWVlCA94NoXPXK-7V2ZsvndVvFyblPMc1VgWdu4H3BXctZvQLYFU1Naa1DHKzNfKrOJGcStwPiXwCZztiuvkyVm3xJbYw1wswXmhS7NUq1Lk2dxpTZ5abRRjUnbdv_df_0jPmtkVrNUGYwXZW2KRtw_330CDyWMkg</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Freitas, Elizabeth M</creator><creator>Zhang, Wen Jie</creator><creator>Lalonde, Jean-Philippe</creator><creator>Tay, Guan K.</creator><creator>Gaudieri, Silvana</creator><creator>Ashworth, Linda K</creator><creator>Dawkins, Roger L</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>Sequencing of 42kb of the APO E-C2 Gene Cluster Reveals a New Gene: PEREC1</title><author>Freitas, Elizabeth M ; Zhang, Wen Jie ; Lalonde, Jean-Philippe ; Tay, Guan K. ; Gaudieri, Silvana ; Ashworth, Linda K ; Dawkins, Roger L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-131c309508dbdeafc013d303af8a1bc1bf93e4b584e2f1ed70eda4a4404702f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alzheimer Disease - genetics</topic><topic>Amino Acid Motifs</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Apolipoproteins E - chemistry</topic><topic>Apolipoproteins E - genetics</topic><topic>Base Sequence</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Cell Adhesion Molecules</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 19 - genetics</topic><topic>Coronary Disease - genetics</topic><topic>Cosmids - genetics</topic><topic>Databases, Factual</topic><topic>Expressed Sequence Tags</topic><topic>Helminth Proteins - chemistry</topic><topic>Helminth Proteins - genetics</topic><topic>Humans</topic><topic>Membrane Glycoproteins - chemistry</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Transport Proteins</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Nectins</topic><topic>Proteins - chemistry</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Receptors, Tumor Necrosis Factor</topic><topic>Receptors, Tumor Necrosis Factor, Member 14</topic><topic>Receptors, Virus</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Analysis, Protein</topic><toplevel>online_resources</toplevel><creatorcontrib>Freitas, Elizabeth M</creatorcontrib><creatorcontrib>Zhang, Wen Jie</creatorcontrib><creatorcontrib>Lalonde, Jean-Philippe</creatorcontrib><creatorcontrib>Tay, Guan K.</creatorcontrib><creatorcontrib>Gaudieri, Silvana</creatorcontrib><creatorcontrib>Ashworth, Linda K</creatorcontrib><creatorcontrib>Dawkins, Roger L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>DNA sequence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freitas, Elizabeth M</au><au>Zhang, Wen Jie</au><au>Lalonde, Jean-Philippe</au><au>Tay, Guan K.</au><au>Gaudieri, Silvana</au><au>Ashworth, Linda K</au><au>Dawkins, Roger L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequencing of 42kb of the APO E-C2 Gene Cluster Reveals a New Gene: PEREC1</atitle><jtitle>DNA sequence</jtitle><addtitle>DNA Seq</addtitle><date>1998</date><risdate>1998</risdate><volume>9</volume><issue>2</issue><spage>89</spage><epage>100</epage><pages>89-100</pages><issn>1042-5179</issn><eissn>1029-2365</eissn><abstract>Through the sequencing of a 42kb cosmid clone we describe a new gene, designated PEREC1, located approximately 1.5kb centromeric of the human apolipoprotein (APO) E-C2 cluster. The combination of dotplot analysis, predicted coding potential and interrogation of the Expressed Sequence Tag (EST) database determined the genomic organisation of PEREC1. Sequence alignment with multiple overlapping ESTs confirmed the predicted splice sites. The predicted cDNA and amino acid sequences of PEREC1 have extensive similarity to the Caenorhabditis elegans protein, C18E9.6. Conserved structural and functional motifs have been defined by combining nucleotide and amino acid analyses to identify third base degeneracy and therefore selection at the protein level.
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| subjects | Alzheimer Disease - genetics Amino Acid Motifs Amino Acid Sequence Animals Apolipoproteins E - chemistry Apolipoproteins E - genetics Base Sequence Caenorhabditis elegans - genetics Cell Adhesion Molecules Chromosome Mapping Chromosomes, Human, Pair 19 - genetics Coronary Disease - genetics Cosmids - genetics Databases, Factual Expressed Sequence Tags Helminth Proteins - chemistry Helminth Proteins - genetics Humans Membrane Glycoproteins - chemistry Membrane Glycoproteins - genetics Membrane Transport Proteins Molecular Sequence Data Multigene Family Nectins Proteins - chemistry Proteins - genetics Proteins - metabolism Receptors, Tumor Necrosis Factor Receptors, Tumor Necrosis Factor, Member 14 Receptors, Virus Sequence Analysis, DNA Sequence Analysis, Protein |
| title | Sequencing of 42kb of the APO E-C2 Gene Cluster Reveals a New Gene: PEREC1 |
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