Genetic variability and mRNA editing frequencies of the phosphoprotein genes of wild-type measles viruses
The sequences of the nucleoprotein (N) and hemagglutinin (H) genes are routinely used for molecular epidemiologic studies of measles virus (MV). However, the amount of genetic diversity contained in other genes of MV has not been thoroughly evaluated. In this report, the nucleotide sequences of the...
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| Veröffentlicht in: | Virus research 2008-08, Vol.135 (2), p.298-306 |
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| creator | Bankamp, B. Lopareva, E.N. Kremer, J.R. Tian, Y. Clemens, M.S. Patel, R. Fowlkes, A.L. Kessler, J.R. Muller, C.P. Bellini, W.J. Rota, P.A. |
| description | The sequences of the nucleoprotein (N) and hemagglutinin (H) genes are routinely used for molecular epidemiologic studies of measles virus (MV). However, the amount of genetic diversity contained in other genes of MV has not been thoroughly evaluated. In this report, the nucleotide sequences of the phosphoprotein (P) genes from 34 wild-type strains representing 15 genotypes of MV were analyzed and found to be almost as variable as the H genes but less variable than the N genes. Deduced amino acid sequences of the three proteins encoded by the P gene, P, V and C, demonstrated considerably higher variability than the H proteins. Phylogenetic analysis showed the same tree topography for the P gene sequences as previously seen for the N and H genes. RNA editing of P gene transcripts affects the relative ratios of P and V proteins, which may have consequences for pathogenicity. Wild-type isolates produced more transcripts with more than one G insertion; however, there was no significant difference in the use of P and V open reading frames, suggesting that the relative amounts of P and V proteins in infected cells would be similar for both vaccine and wild-type strains. |
| doi_str_mv | 10.1016/j.virusres.2008.04.008 |
| format | Article |
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However, the amount of genetic diversity contained in other genes of MV has not been thoroughly evaluated. In this report, the nucleotide sequences of the phosphoprotein (P) genes from 34 wild-type strains representing 15 genotypes of MV were analyzed and found to be almost as variable as the H genes but less variable than the N genes. Deduced amino acid sequences of the three proteins encoded by the P gene, P, V and C, demonstrated considerably higher variability than the H proteins. Phylogenetic analysis showed the same tree topography for the P gene sequences as previously seen for the N and H genes. RNA editing of P gene transcripts affects the relative ratios of P and V proteins, which may have consequences for pathogenicity. Wild-type isolates produced more transcripts with more than one G insertion; however, there was no significant difference in the use of P and V open reading frames, suggesting that the relative amounts of P and V proteins in infected cells would be similar for both vaccine and wild-type strains.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2008.04.008</identifier><identifier>PMID: 18490071</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antigens, CD - metabolism ; Cercopithecus aethiops ; Genetic Variation ; Genotype ; Humans ; Measles ; Measles virus ; Measles virus - classification ; Measles virus - genetics ; Molecular Sequence Data ; Phosphoprotein ; Phosphoproteins - chemistry ; Phosphoproteins - genetics ; Phosphoproteins - metabolism ; Phylogeny ; Receptors, Cell Surface - metabolism ; RNA Editing ; Sequence Analysis, DNA ; Signaling Lymphocytic Activation Molecule Family Member 1 ; Vero Cells ; Viral Proteins - chemistry ; Viral Proteins - genetics ; Viral Proteins - metabolism</subject><ispartof>Virus research, 2008-08, Vol.135 (2), p.298-306</ispartof><rights>2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-444099acd64d722373e5cf82a17eb04995b87aa604bf1a46f07e216b60872fc73</citedby><cites>FETCH-LOGICAL-c463t-444099acd64d722373e5cf82a17eb04995b87aa604bf1a46f07e216b60872fc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S016817020800141X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18490071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bankamp, B.</creatorcontrib><creatorcontrib>Lopareva, E.N.</creatorcontrib><creatorcontrib>Kremer, J.R.</creatorcontrib><creatorcontrib>Tian, Y.</creatorcontrib><creatorcontrib>Clemens, M.S.</creatorcontrib><creatorcontrib>Patel, R.</creatorcontrib><creatorcontrib>Fowlkes, A.L.</creatorcontrib><creatorcontrib>Kessler, J.R.</creatorcontrib><creatorcontrib>Muller, C.P.</creatorcontrib><creatorcontrib>Bellini, W.J.</creatorcontrib><creatorcontrib>Rota, P.A.</creatorcontrib><title>Genetic variability and mRNA editing frequencies of the phosphoprotein genes of wild-type measles viruses</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>The sequences of the nucleoprotein (N) and hemagglutinin (H) genes are routinely used for molecular epidemiologic studies of measles virus (MV). However, the amount of genetic diversity contained in other genes of MV has not been thoroughly evaluated. In this report, the nucleotide sequences of the phosphoprotein (P) genes from 34 wild-type strains representing 15 genotypes of MV were analyzed and found to be almost as variable as the H genes but less variable than the N genes. Deduced amino acid sequences of the three proteins encoded by the P gene, P, V and C, demonstrated considerably higher variability than the H proteins. Phylogenetic analysis showed the same tree topography for the P gene sequences as previously seen for the N and H genes. RNA editing of P gene transcripts affects the relative ratios of P and V proteins, which may have consequences for pathogenicity. 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However, the amount of genetic diversity contained in other genes of MV has not been thoroughly evaluated. In this report, the nucleotide sequences of the phosphoprotein (P) genes from 34 wild-type strains representing 15 genotypes of MV were analyzed and found to be almost as variable as the H genes but less variable than the N genes. Deduced amino acid sequences of the three proteins encoded by the P gene, P, V and C, demonstrated considerably higher variability than the H proteins. Phylogenetic analysis showed the same tree topography for the P gene sequences as previously seen for the N and H genes. RNA editing of P gene transcripts affects the relative ratios of P and V proteins, which may have consequences for pathogenicity. 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| subjects | Animals Antigens, CD - metabolism Cercopithecus aethiops Genetic Variation Genotype Humans Measles Measles virus Measles virus - classification Measles virus - genetics Molecular Sequence Data Phosphoprotein Phosphoproteins - chemistry Phosphoproteins - genetics Phosphoproteins - metabolism Phylogeny Receptors, Cell Surface - metabolism RNA Editing Sequence Analysis, DNA Signaling Lymphocytic Activation Molecule Family Member 1 Vero Cells Viral Proteins - chemistry Viral Proteins - genetics Viral Proteins - metabolism |
| title | Genetic variability and mRNA editing frequencies of the phosphoprotein genes of wild-type measles viruses |
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