The role of leaders in intracellular transport and secretion of the insulin precursor in the yeast Saccharomyces cerevisiae

Pulse-chase analysis of folded and misfolded insulin precursor (IP) expressed in Saccharomyces cerevisiae was performed to establish the requirements for intracellular transport and the influence of the secretory pathway quality control mechanisms on secretion. Metabolic labelling of the IP expresse...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of biotechnology 1999-10, Vol.75 (2), p.195-208
Hauptverfasser:	Kjeldsen, Thomas, Frost Pettersson, Annette, Hach, Morten
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Aspartic Acid Endopeptidases - genetics Aspartic Acid Endopeptidases - metabolism Biological and medical sciences Biological Transport - drug effects Biotechnology Cells Chemical bonds Disulphide bond Dithiothreitol - pharmacology Electrophoresis, Gel, Pulsed-Field - methods Endoplasmic Reticulum - metabolism Enzymes Escherichia coli Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Golgi Apparatus - metabolism Hormone precursor Insulin - metabolism Insulin Secretion Kinetics Mating Factor Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Peptides - genetics Peptides - metabolism Pro-peptide Protein Folding Protein Precursors - genetics Protein Precursors - metabolism Proteins Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombinant protein Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins Secretory pathway Yeast
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!