Mimicking rubella virus particles by using recombinant envelope glycoproteins and liposomes

The envelope glycoproteins E1 and E2 of rubella virus (RV) were engineered to display the FLAG epitope tag and a polyhistidine tag, at their amino and carboxy termini, respectively. These modified envelope proteins were produced in Sf9 insect cells utilizing baculovirus expression vectors, the E1 an...

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Veröffentlicht in:	Journal of biotechnology 1999-10, Vol.75 (2), p.209-219
Hauptverfasser:	Orellana, Adelina, Mottershead, David, van der Linden, Inge, Keinänen, Kari, Oker-Blom, Christian
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies Antigens, Viral - genetics Baculovirus Biological and medical sciences Biotechnology Cells Chelating Agents - chemistry Chromatography Diagnostic products Dialysis Diseases Envelope proteins Europium - chemistry Europium-DTPA Fundamental and applied biological sciences. Psychology Glycoproteins - genetics Health. Pharmaceutical industry Immunoblotting Industrial applications and implications. Economical aspects Insect cells Lepidoptera - cytology Lepidoptera - genetics Liposomes Liposomes - chemistry Miscellaneous Molecular Mimicry Molecular Sequence Data Pentetic Acid - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Rubella - diagnosis Rubella virus Rubella virus - genetics Rubella virus - immunology Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Viral Envelope Proteins - isolation & purification
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