Enhancement of nasal absorption of insulin using chitosan nanoparticles
To investigate the potential of chitosan nanoparticles as a system for improving the systemic absorption of insulin following nasal instillation. Insulin-loaded chitosan nanoparticles were prepared by ionotropic gelation of chitosan with tripolyphosphate anions. They were characterized for their siz...
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| Veröffentlicht in: | Pharmaceutical research 1999-10, Vol.16 (10), p.1576-1581 |
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| creator | FERNANDEZ-URRUSUNO, R CALVO, P REMUNAN-LOPEZ, C VILA-JATO, J. L ALONSO, M. J |
| description | To investigate the potential of chitosan nanoparticles as a system for improving the systemic absorption of insulin following nasal instillation.
Insulin-loaded chitosan nanoparticles were prepared by ionotropic gelation of chitosan with tripolyphosphate anions. They were characterized for their size and zeta potential by photon correlation spectroscopy and laser Doppler anemometry, respectively. Insulin loading and release was determined by the microBCA protein assay. The ability of chitosan nanoparticles to enhance the nasal absorption of insulin was investigated in a conscious rabbit model by monitoring the plasma glucose levels.
Chitosan nanoparticles had a size in the range of 300-400 nm, a positive surface charge and their insulin loading can be modulated reaching values up to 55% [insulin/nanoparticles (w/w): 55/100]. Insulin association was found to be highly mediated by an ionic interaction mechanism and its release in vitro occurred rapidly in sink conditions. Chitosan nanoparticles enhanced the nasal absorption of insulin to a greater extent than an aqueous solution of chitosan. The amount and molecular weight of chitosan did not have a significant effect on insulin response.
Chitosan nanoparticles are efficient vehicles for the transport of insulin through the nasal mucosa. |
| doi_str_mv | 10.1023/A:1018908705446 |
| format | Article |
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Insulin-loaded chitosan nanoparticles were prepared by ionotropic gelation of chitosan with tripolyphosphate anions. They were characterized for their size and zeta potential by photon correlation spectroscopy and laser Doppler anemometry, respectively. Insulin loading and release was determined by the microBCA protein assay. The ability of chitosan nanoparticles to enhance the nasal absorption of insulin was investigated in a conscious rabbit model by monitoring the plasma glucose levels.
Chitosan nanoparticles had a size in the range of 300-400 nm, a positive surface charge and their insulin loading can be modulated reaching values up to 55% [insulin/nanoparticles (w/w): 55/100]. Insulin association was found to be highly mediated by an ionic interaction mechanism and its release in vitro occurred rapidly in sink conditions. Chitosan nanoparticles enhanced the nasal absorption of insulin to a greater extent than an aqueous solution of chitosan. The amount and molecular weight of chitosan did not have a significant effect on insulin response.
Chitosan nanoparticles are efficient vehicles for the transport of insulin through the nasal mucosa.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1023/A:1018908705446</identifier><identifier>PMID: 10554100</identifier><identifier>CODEN: PHREEB</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Absorption ; Administration, Intranasal ; Animals ; Biological and medical sciences ; Blood Glucose - drug effects ; Blood Glucose - metabolism ; Chemical Phenomena ; Chemistry, Physical ; Chitin - analogs & derivatives ; Chitin - pharmacology ; Chitosan ; Excipients ; General and cellular metabolism. Vitamins ; General pharmacology ; Hypoglycemic Agents - administration & dosage ; Hypoglycemic Agents - pharmacokinetics ; Hypoglycemic Agents - pharmacology ; Insulin - administration & dosage ; Insulin - pharmacokinetics ; Insulin - pharmacology ; Male ; Medical sciences ; Microspheres ; Nasal Mucosa - drug effects ; Nasal Mucosa - metabolism ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Rabbits ; Stimulation, Chemical</subject><ispartof>Pharmaceutical research, 1999-10, Vol.16 (10), p.1576-1581</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright Kluwer Academic Publishers Oct 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c309t-dfd5fa0d5cff2ecc8a19424fa3bf01427e734cb0d2f0d00f3c9dcfd48d9984c23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1989895$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10554100$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FERNANDEZ-URRUSUNO, R</creatorcontrib><creatorcontrib>CALVO, P</creatorcontrib><creatorcontrib>REMUNAN-LOPEZ, C</creatorcontrib><creatorcontrib>VILA-JATO, J. L</creatorcontrib><creatorcontrib>ALONSO, M. J</creatorcontrib><title>Enhancement of nasal absorption of insulin using chitosan nanoparticles</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><description>To investigate the potential of chitosan nanoparticles as a system for improving the systemic absorption of insulin following nasal instillation.
Insulin-loaded chitosan nanoparticles were prepared by ionotropic gelation of chitosan with tripolyphosphate anions. They were characterized for their size and zeta potential by photon correlation spectroscopy and laser Doppler anemometry, respectively. Insulin loading and release was determined by the microBCA protein assay. The ability of chitosan nanoparticles to enhance the nasal absorption of insulin was investigated in a conscious rabbit model by monitoring the plasma glucose levels.
Chitosan nanoparticles had a size in the range of 300-400 nm, a positive surface charge and their insulin loading can be modulated reaching values up to 55% [insulin/nanoparticles (w/w): 55/100]. Insulin association was found to be highly mediated by an ionic interaction mechanism and its release in vitro occurred rapidly in sink conditions. Chitosan nanoparticles enhanced the nasal absorption of insulin to a greater extent than an aqueous solution of chitosan. The amount and molecular weight of chitosan did not have a significant effect on insulin response.
Chitosan nanoparticles are efficient vehicles for the transport of insulin through the nasal mucosa.</description><subject>Absorption</subject><subject>Administration, Intranasal</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - drug effects</subject><subject>Blood Glucose - metabolism</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Chitin - analogs & derivatives</subject><subject>Chitin - pharmacology</subject><subject>Chitosan</subject><subject>Excipients</subject><subject>General and cellular metabolism. Vitamins</subject><subject>General pharmacology</subject><subject>Hypoglycemic Agents - administration & dosage</subject><subject>Hypoglycemic Agents - pharmacokinetics</subject><subject>Hypoglycemic Agents - pharmacology</subject><subject>Insulin - administration & dosage</subject><subject>Insulin - pharmacokinetics</subject><subject>Insulin - pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microspheres</subject><subject>Nasal Mucosa - drug effects</subject><subject>Nasal Mucosa - metabolism</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbits</subject><subject>Stimulation, Chemical</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0E1Lw0AQBuBFFFurZ28SRLxFZz-S7HorpVah4EXBW9jsh92S7MZscvDfm2JEkDkMDA_Dy4vQJYY7DITeLx8wYC6AF5Axlh-hOc4Kmgpg78doDgVhKS8YnqGzGPcAwLFgp2iGIcsYBpijzdrvpFemMb5Pgk28jLJOZBVD1_Yu-MPN-TjUzidDdP4jUTvXhyj9SH1oZdc7VZt4jk6srKO5mPYCvT2uX1dP6fZl87xablNFQfSptjqzEnSmrCVGKS7HQIRZSSsLmJHCFJSpCjSxoAEsVUIrqxnXQnCmCF2g25-_bRc-BxP7snFRmbqW3oQhlrkgjBYsH-H1P7gPQ-fHbCUhJM8JJwd0NaGhaowu2841svsqf_sZwc0EZFSytt3YlYt_TvBxMvoNiSx0wg</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>FERNANDEZ-URRUSUNO, R</creator><creator>CALVO, P</creator><creator>REMUNAN-LOPEZ, C</creator><creator>VILA-JATO, J. L</creator><creator>ALONSO, M. 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J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c309t-dfd5fa0d5cff2ecc8a19424fa3bf01427e734cb0d2f0d00f3c9dcfd48d9984c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Absorption</topic><topic>Administration, Intranasal</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - drug effects</topic><topic>Blood Glucose - metabolism</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Chitin - analogs & derivatives</topic><topic>Chitin - pharmacology</topic><topic>Chitosan</topic><topic>Excipients</topic><topic>General and cellular metabolism. Vitamins</topic><topic>General pharmacology</topic><topic>Hypoglycemic Agents - administration & dosage</topic><topic>Hypoglycemic Agents - pharmacokinetics</topic><topic>Hypoglycemic Agents - pharmacology</topic><topic>Insulin - administration & dosage</topic><topic>Insulin - pharmacokinetics</topic><topic>Insulin - pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microspheres</topic><topic>Nasal Mucosa - drug effects</topic><topic>Nasal Mucosa - metabolism</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbits</topic><topic>Stimulation, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FERNANDEZ-URRUSUNO, R</creatorcontrib><creatorcontrib>CALVO, P</creatorcontrib><creatorcontrib>REMUNAN-LOPEZ, C</creatorcontrib><creatorcontrib>VILA-JATO, J. L</creatorcontrib><creatorcontrib>ALONSO, M. 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L</au><au>ALONSO, M. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of nasal absorption of insulin using chitosan nanoparticles</atitle><jtitle>Pharmaceutical research</jtitle><addtitle>Pharm Res</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>16</volume><issue>10</issue><spage>1576</spage><epage>1581</epage><pages>1576-1581</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><coden>PHREEB</coden><abstract>To investigate the potential of chitosan nanoparticles as a system for improving the systemic absorption of insulin following nasal instillation.
Insulin-loaded chitosan nanoparticles were prepared by ionotropic gelation of chitosan with tripolyphosphate anions. They were characterized for their size and zeta potential by photon correlation spectroscopy and laser Doppler anemometry, respectively. Insulin loading and release was determined by the microBCA protein assay. The ability of chitosan nanoparticles to enhance the nasal absorption of insulin was investigated in a conscious rabbit model by monitoring the plasma glucose levels.
Chitosan nanoparticles had a size in the range of 300-400 nm, a positive surface charge and their insulin loading can be modulated reaching values up to 55% [insulin/nanoparticles (w/w): 55/100]. Insulin association was found to be highly mediated by an ionic interaction mechanism and its release in vitro occurred rapidly in sink conditions. Chitosan nanoparticles enhanced the nasal absorption of insulin to a greater extent than an aqueous solution of chitosan. The amount and molecular weight of chitosan did not have a significant effect on insulin response.
Chitosan nanoparticles are efficient vehicles for the transport of insulin through the nasal mucosa.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>10554100</pmid><doi>10.1023/A:1018908705446</doi><tpages>6</tpages></addata></record> |
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| subjects | Absorption Administration, Intranasal Animals Biological and medical sciences Blood Glucose - drug effects Blood Glucose - metabolism Chemical Phenomena Chemistry, Physical Chitin - analogs & derivatives Chitin - pharmacology Chitosan Excipients General and cellular metabolism. Vitamins General pharmacology Hypoglycemic Agents - administration & dosage Hypoglycemic Agents - pharmacokinetics Hypoglycemic Agents - pharmacology Insulin - administration & dosage Insulin - pharmacokinetics Insulin - pharmacology Male Medical sciences Microspheres Nasal Mucosa - drug effects Nasal Mucosa - metabolism Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Rabbits Stimulation, Chemical |
| title | Enhancement of nasal absorption of insulin using chitosan nanoparticles |
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