Isolation and characterization of an iridovirus from Hermann's tortoises (Testudo hermanni)
A virus was isolated from tissues of 2 diseased Hermann’s tortoises (Testudo hermanni) and preliminarily characterized as an iridovirus. This conclusion was based on the presence of inclusion bodies in the cytoplasm of infected cells, sensitivity to chloroform, inhibition of virus replication by 5-i...
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| Veröffentlicht in: | Archives of virology 1999-01, Vol.144 (10), p.1909-1922 |
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| container_end_page | 1922 |
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| container_issue | 10 |
| container_start_page | 1909 |
| container_title | Archives of virology |
| container_volume | 144 |
| creator | Marschang, R.E Becher, P Posthaus, H Wild, P Thiel, H.J Muller-Doblies, U Kaleta, E.F Bacciarini, L.N |
| description | A virus was isolated from tissues of 2 diseased Hermann’s tortoises (Testudo hermanni) and preliminarily characterized as an iridovirus. This conclusion was based on the presence of inclusion bodies in the cytoplasm of infected cells, sensitivity to chloroform, inhibition of virus replication by 5-iodo-2′-desoxyuridine and the size and icosahedral morphology of viral particles. The virus was able to replicate in several reptilian, avian and mammalian cell lines at 28°C, but not at 37°C. Restriction enzyme analysis showed resistance of the ral DNA to digestion with HpaII due to methylation of the internal cytosine at CCGG sequences. Part of the genomic region encoding the major capsid protein was amplified by PCR and subjected to sequence analysis. Comparative analysis of the obtained nucleotide sequence revealed that the isolate is closely related to frog virus 3, the type species of the genus Ranavirus. |
| doi_str_mv | 10.1007/s007050050714 |
| format | Article |
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This conclusion was based on the presence of inclusion bodies in the cytoplasm of infected cells, sensitivity to chloroform, inhibition of virus replication by 5-iodo-2′-desoxyuridine and the size and icosahedral morphology of viral particles. The virus was able to replicate in several reptilian, avian and mammalian cell lines at 28°C, but not at 37°C. Restriction enzyme analysis showed resistance of the ral DNA to digestion with HpaII due to methylation of the internal cytosine at CCGG sequences. Part of the genomic region encoding the major capsid protein was amplified by PCR and subjected to sequence analysis. Comparative analysis of the obtained nucleotide sequence revealed that the isolate is closely related to frog virus 3, the type species of the genus Ranavirus.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s007050050714</identifier><identifier>PMID: 10550665</identifier><language>eng</language><publisher>Wien: Springer-Verlag</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animals ; Biological and medical sciences ; Capsid - genetics ; Cell Line ; Cloning, Molecular ; coat proteins ; cytoplasm ; cytoplasmic inclusions ; cytosine ; DNA ; DNA Restriction Enzymes - metabolism ; DNA Virus Infections - pathology ; DNA Virus Infections - veterinary ; DNA Virus Infections - virology ; DNA, Viral - analysis ; DNA, Viral - genetics ; Freshwater ; Frog virus 3 ; Fundamental and applied biological sciences. Psychology ; genbank/af114154 ; inclusion bodies ; Iridovirus ; Iridovirus - classification ; Iridovirus - genetics ; Iridovirus - isolation & purification ; Iridovirus - ultrastructure ; methylation ; Microbiology ; Microscopy, Electron ; Molecular Sequence Data ; nucleotide sequences ; Phylogeny ; Polymerase Chain Reaction ; Ranavirus ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; restriction mapping ; sequence analysis ; Sequence Analysis, DNA ; Testudo ; Testudo hermanni ; tortoises ; Turtles - virology ; viral morphology ; Virology ; virus replication ; viruses</subject><ispartof>Archives of virology, 1999-01, Vol.144 (10), p.1909-1922</ispartof><rights>2000 INIST-CNRS</rights><rights>1999 Springer-Verlag/ Wien</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-4804322d64a021769e1a4bd7896a93103a7059f19bcc1ba220d11644ac0d65b73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1190601$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10550665$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marschang, R.E</creatorcontrib><creatorcontrib>Becher, P</creatorcontrib><creatorcontrib>Posthaus, H</creatorcontrib><creatorcontrib>Wild, P</creatorcontrib><creatorcontrib>Thiel, H.J</creatorcontrib><creatorcontrib>Muller-Doblies, U</creatorcontrib><creatorcontrib>Kaleta, E.F</creatorcontrib><creatorcontrib>Bacciarini, L.N</creatorcontrib><title>Isolation and characterization of an iridovirus from Hermann's tortoises (Testudo hermanni)</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>A virus was isolated from tissues of 2 diseased Hermann’s tortoises (Testudo hermanni) and preliminarily characterized as an iridovirus. This conclusion was based on the presence of inclusion bodies in the cytoplasm of infected cells, sensitivity to chloroform, inhibition of virus replication by 5-iodo-2′-desoxyuridine and the size and icosahedral morphology of viral particles. The virus was able to replicate in several reptilian, avian and mammalian cell lines at 28°C, but not at 37°C. Restriction enzyme analysis showed resistance of the ral DNA to digestion with HpaII due to methylation of the internal cytosine at CCGG sequences. Part of the genomic region encoding the major capsid protein was amplified by PCR and subjected to sequence analysis. Comparative analysis of the obtained nucleotide sequence revealed that the isolate is closely related to frog virus 3, the type species of the genus Ranavirus.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Capsid - genetics</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>coat proteins</subject><subject>cytoplasm</subject><subject>cytoplasmic inclusions</subject><subject>cytosine</subject><subject>DNA</subject><subject>DNA Restriction Enzymes - metabolism</subject><subject>DNA Virus Infections - pathology</subject><subject>DNA Virus Infections - veterinary</subject><subject>DNA Virus Infections - virology</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - genetics</subject><subject>Freshwater</subject><subject>Frog virus 3</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/af114154</subject><subject>inclusion bodies</subject><subject>Iridovirus</subject><subject>Iridovirus - classification</subject><subject>Iridovirus - genetics</subject><subject>Iridovirus - isolation & purification</subject><subject>Iridovirus - ultrastructure</subject><subject>methylation</subject><subject>Microbiology</subject><subject>Microscopy, Electron</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Ranavirus</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>restriction mapping</subject><subject>sequence analysis</subject><subject>Sequence Analysis, DNA</subject><subject>Testudo</subject><subject>Testudo hermanni</subject><subject>tortoises</subject><subject>Turtles - virology</subject><subject>viral morphology</subject><subject>Virology</subject><subject>virus replication</subject><subject>viruses</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0strFjEQAPAgiv1aPXrVBYuPw-pMnpujFLWFggfbk4dlNpu1KbubmuwK-tebsh_4ACmEBGZ-DDNMGHuC8AYBzNtcLlBQjkF5j-1QCl43xjb32Q4EyLrR0Byww5yvAUpAqIfsAEEp0Frt2JezHEdaQpwrmvvKXVEit_gUfm7BOJR4FVLo4_eQ1lwNKU7VqU8TzfPLXC0xLTFkn6tXFz4vax-rqy0ZXj9iDwYas3-8f4_Y5Yf3Fyen9fmnj2cn785rpzhfatlA6Zn3WhJwNNp6JNn1prGarEAQVAa0A9rOOeyIc-gRtZTkoNeqM-KIvdjq3qT4bS1dtFPIzo8jzT6uudWWSyGVvhNiIyUK5HdDIxrDtSrw-T_wOq5pLtO2CFxqK429bbDelEsx5-SH9iaFidKPgtrbLbZ_bbH4p_uqazf5_g-9ra2A4z2g7GgcEs0u5N8OLWjAwp5tbKDY0tdUyOVnDijLT2iMVPa_QgC3UlgQ4hfxGrPN</recordid><startdate>19990101</startdate><enddate>19990101</enddate><creator>Marschang, R.E</creator><creator>Becher, P</creator><creator>Posthaus, H</creator><creator>Wild, P</creator><creator>Thiel, H.J</creator><creator>Muller-Doblies, U</creator><creator>Kaleta, E.F</creator><creator>Bacciarini, L.N</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>19990101</creationdate><title>Isolation and characterization of an iridovirus from Hermann's tortoises (Testudo hermanni)</title><author>Marschang, R.E ; Becher, P ; Posthaus, H ; Wild, P ; Thiel, H.J ; Muller-Doblies, U ; Kaleta, E.F ; Bacciarini, L.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-4804322d64a021769e1a4bd7896a93103a7059f19bcc1ba220d11644ac0d65b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Capsid - genetics</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>coat proteins</topic><topic>cytoplasm</topic><topic>cytoplasmic inclusions</topic><topic>cytosine</topic><topic>DNA</topic><topic>DNA Restriction Enzymes - metabolism</topic><topic>DNA Virus Infections - pathology</topic><topic>DNA Virus Infections - veterinary</topic><topic>DNA Virus Infections - virology</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - genetics</topic><topic>Freshwater</topic><topic>Frog virus 3</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/af114154</topic><topic>inclusion bodies</topic><topic>Iridovirus</topic><topic>Iridovirus - classification</topic><topic>Iridovirus - genetics</topic><topic>Iridovirus - isolation & purification</topic><topic>Iridovirus - ultrastructure</topic><topic>methylation</topic><topic>Microbiology</topic><topic>Microscopy, Electron</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Ranavirus</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>restriction mapping</topic><topic>sequence analysis</topic><topic>Sequence Analysis, DNA</topic><topic>Testudo</topic><topic>Testudo hermanni</topic><topic>tortoises</topic><topic>Turtles - virology</topic><topic>viral morphology</topic><topic>Virology</topic><topic>virus replication</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marschang, R.E</creatorcontrib><creatorcontrib>Becher, P</creatorcontrib><creatorcontrib>Posthaus, H</creatorcontrib><creatorcontrib>Wild, P</creatorcontrib><creatorcontrib>Thiel, H.J</creatorcontrib><creatorcontrib>Muller-Doblies, U</creatorcontrib><creatorcontrib>Kaleta, E.F</creatorcontrib><creatorcontrib>Bacciarini, L.N</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marschang, R.E</au><au>Becher, P</au><au>Posthaus, H</au><au>Wild, P</au><au>Thiel, H.J</au><au>Muller-Doblies, U</au><au>Kaleta, E.F</au><au>Bacciarini, L.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of an iridovirus from Hermann's tortoises (Testudo hermanni)</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>144</volume><issue>10</issue><spage>1909</spage><epage>1922</epage><pages>1909-1922</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>A virus was isolated from tissues of 2 diseased Hermann’s tortoises (Testudo hermanni) and preliminarily characterized as an iridovirus. This conclusion was based on the presence of inclusion bodies in the cytoplasm of infected cells, sensitivity to chloroform, inhibition of virus replication by 5-iodo-2′-desoxyuridine and the size and icosahedral morphology of viral particles. The virus was able to replicate in several reptilian, avian and mammalian cell lines at 28°C, but not at 37°C. Restriction enzyme analysis showed resistance of the ral DNA to digestion with HpaII due to methylation of the internal cytosine at CCGG sequences. Part of the genomic region encoding the major capsid protein was amplified by PCR and subjected to sequence analysis. Comparative analysis of the obtained nucleotide sequence revealed that the isolate is closely related to frog virus 3, the type species of the genus Ranavirus.</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer-Verlag</pub><pmid>10550665</pmid><doi>10.1007/s007050050714</doi><tpages>14</tpages></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Animals Biological and medical sciences Capsid - genetics Cell Line Cloning, Molecular coat proteins cytoplasm cytoplasmic inclusions cytosine DNA DNA Restriction Enzymes - metabolism DNA Virus Infections - pathology DNA Virus Infections - veterinary DNA Virus Infections - virology DNA, Viral - analysis DNA, Viral - genetics Freshwater Frog virus 3 Fundamental and applied biological sciences. Psychology genbank/af114154 inclusion bodies Iridovirus Iridovirus - classification Iridovirus - genetics Iridovirus - isolation & purification Iridovirus - ultrastructure methylation Microbiology Microscopy, Electron Molecular Sequence Data nucleotide sequences Phylogeny Polymerase Chain Reaction Ranavirus Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains restriction mapping sequence analysis Sequence Analysis, DNA Testudo Testudo hermanni tortoises Turtles - virology viral morphology Virology virus replication viruses |
| title | Isolation and characterization of an iridovirus from Hermann's tortoises (Testudo hermanni) |
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