Rescue of a bovine respiratory syncytial virus genomic RNA analog by bovine, human and ovine respiratory syncytial viruses confirms the “functional integrity” and “cross-recognition” of BRSV cis-acting elements by HRSV and ORSV
The nucleotide sequences of the 3′ leader and 5′ trailer regions were determined for genomic RNA of bovine respiratory syncytial virus (BRSV) strain A-51908. The leader and trailer sequences are ‘45’ and ‘161’ nucleotides in length, respectively. The functionality of BRSV leader and trailer sequence...
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Veröffentlicht in: | Archives of virology 1999-01, Vol.144 (10), p.1977-1990 |
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container_end_page | 1990 |
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container_issue | 10 |
container_start_page | 1977 |
container_title | Archives of virology |
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creator | Yunus, A. S Krishnamurthy, S Pastey, M. K Huang, Z Khattar, S. K Collins, P. L Samal, S. K |
description | The nucleotide sequences of the 3′ leader and 5′ trailer regions were determined for genomic RNA of bovine respiratory syncytial virus (BRSV) strain A-51908. The leader and trailer sequences are ‘45’ and ‘161’ nucleotides in length, respectively. The functionality of BRSV leader and trailer sequences and their recognition by HRSV and ovine respiratory syncytial virus (ORSV) proteins were examined with a in vitro transcribed BRSV genomic RNA analog carrying the bacterial chloramphenicol acetyl transferase (CAT) gene under the control of BRSV transcription signals. Upon transfection into BRSV, HRSV or ORSV infected cells, the BRSV minireplicons were ‘rescued’ such that the reporter gene was expressed, the minigenome was replicated and packaged into micrococcal nuclease resistant-infectious minireplicons. The passage of infectious minireplicons could be blocked by a polyclonal BRSV neutralizing antiserum. Bovine parainfluenza virus-3, a heterologous paramyxovirus was inactive in rescuing BRSV genomic RNA analog. Mutational substitution of the G residue at position 4 of leader sequence in the BRSV genomic RNA analog, with an A or U residue inhibited its transcription and replication, while replacement with a C residue had no significant effect on rescue. These results show that the cis-acting elements of BRSV are functional and are also recognized by the proteins of HRSV and ORSV. The helper virus complemented rescue system developed here will be useful for characterizing the cis-acting elements of BRSV. |
doi_str_mv | 10.1007/s007050050719 |
format | Article |
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S ; Krishnamurthy, S ; Pastey, M. K ; Huang, Z ; Khattar, S. K ; Collins, P. L ; Samal, S. K</creator><creatorcontrib>Yunus, A. S ; Krishnamurthy, S ; Pastey, M. K ; Huang, Z ; Khattar, S. K ; Collins, P. L ; Samal, S. K</creatorcontrib><description>The nucleotide sequences of the 3′ leader and 5′ trailer regions were determined for genomic RNA of bovine respiratory syncytial virus (BRSV) strain A-51908. The leader and trailer sequences are ‘45’ and ‘161’ nucleotides in length, respectively. The functionality of BRSV leader and trailer sequences and their recognition by HRSV and ovine respiratory syncytial virus (ORSV) proteins were examined with a in vitro transcribed BRSV genomic RNA analog carrying the bacterial chloramphenicol acetyl transferase (CAT) gene under the control of BRSV transcription signals. Upon transfection into BRSV, HRSV or ORSV infected cells, the BRSV minireplicons were ‘rescued’ such that the reporter gene was expressed, the minigenome was replicated and packaged into micrococcal nuclease resistant-infectious minireplicons. The passage of infectious minireplicons could be blocked by a polyclonal BRSV neutralizing antiserum. Bovine parainfluenza virus-3, a heterologous paramyxovirus was inactive in rescuing BRSV genomic RNA analog. Mutational substitution of the G residue at position 4 of leader sequence in the BRSV genomic RNA analog, with an A or U residue inhibited its transcription and replication, while replacement with a C residue had no significant effect on rescue. These results show that the cis-acting elements of BRSV are functional and are also recognized by the proteins of HRSV and ORSV. The helper virus complemented rescue system developed here will be useful for characterizing the cis-acting elements of BRSV.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s007050050719</identifier><identifier>PMID: 10550670</identifier><language>eng</language><publisher>Wien: Springer-Verlag</publisher><subject>3' Untranslated Regions - genetics ; 5' Untranslated Regions - genetics ; Animals ; antiserum ; Biological and medical sciences ; Blotting, Northern ; Bovine respiratory syncytial virus ; Cattle ; chloramphenicol acetyltransferase ; Chloramphenicol O-Acetyltransferase - genetics ; Chloramphenicol O-Acetyltransferase - metabolism ; Cloning, Molecular ; DNA, Complementary - genetics ; Fundamental and applied biological sciences. Psychology ; Genetics ; Genome, Viral ; Helper Viruses - genetics ; Helper Viruses - metabolism ; Human respiratory syncytial virus ; Humans ; Microbiology ; micrococcal nuclease ; Molecular Sequence Data ; Mutation ; neutralization ; Neutralization Tests ; nucleotide sequences ; Ovine respiratory syncytial virus ; proteins ; Replicon ; reporter genes ; Respiratory syncytial virus ; Respiratory Syncytial Virus, Bovine - genetics ; Respiratory Syncytial Virus, Bovine - physiology ; Respiratory Syncytial Virus, Human - genetics ; Respiratory Syncytial Viruses - genetics ; Respirovirus ; RNA ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; sheep ; Transcription, Genetic ; Transfection ; Virology ; Virus Replication ; viruses</subject><ispartof>Archives of virology, 1999-01, Vol.144 (10), p.1977-1990</ispartof><rights>2000 INIST-CNRS</rights><rights>1999 Springer-Verlag/ Wien</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-e3248bd41d30fc4875836c16daa4baec8ed0e639212831f7365c83103467eba33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1192322$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10550670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yunus, A. S</creatorcontrib><creatorcontrib>Krishnamurthy, S</creatorcontrib><creatorcontrib>Pastey, M. K</creatorcontrib><creatorcontrib>Huang, Z</creatorcontrib><creatorcontrib>Khattar, S. K</creatorcontrib><creatorcontrib>Collins, P. L</creatorcontrib><creatorcontrib>Samal, S. K</creatorcontrib><title>Rescue of a bovine respiratory syncytial virus genomic RNA analog by bovine, human and ovine respiratory syncytial viruses confirms the “functional integrity” and “cross-recognition” of BRSV cis-acting elements by HRSV and ORSV</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>The nucleotide sequences of the 3′ leader and 5′ trailer regions were determined for genomic RNA of bovine respiratory syncytial virus (BRSV) strain A-51908. The leader and trailer sequences are ‘45’ and ‘161’ nucleotides in length, respectively. The functionality of BRSV leader and trailer sequences and their recognition by HRSV and ovine respiratory syncytial virus (ORSV) proteins were examined with a in vitro transcribed BRSV genomic RNA analog carrying the bacterial chloramphenicol acetyl transferase (CAT) gene under the control of BRSV transcription signals. Upon transfection into BRSV, HRSV or ORSV infected cells, the BRSV minireplicons were ‘rescued’ such that the reporter gene was expressed, the minigenome was replicated and packaged into micrococcal nuclease resistant-infectious minireplicons. The passage of infectious minireplicons could be blocked by a polyclonal BRSV neutralizing antiserum. Bovine parainfluenza virus-3, a heterologous paramyxovirus was inactive in rescuing BRSV genomic RNA analog. Mutational substitution of the G residue at position 4 of leader sequence in the BRSV genomic RNA analog, with an A or U residue inhibited its transcription and replication, while replacement with a C residue had no significant effect on rescue. These results show that the cis-acting elements of BRSV are functional and are also recognized by the proteins of HRSV and ORSV. The helper virus complemented rescue system developed here will be useful for characterizing the cis-acting elements of BRSV.</description><subject>3' Untranslated Regions - genetics</subject><subject>5' Untranslated Regions - genetics</subject><subject>Animals</subject><subject>antiserum</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Bovine respiratory syncytial virus</subject><subject>Cattle</subject><subject>chloramphenicol acetyltransferase</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Genome, Viral</subject><subject>Helper Viruses - genetics</subject><subject>Helper Viruses - metabolism</subject><subject>Human respiratory syncytial virus</subject><subject>Humans</subject><subject>Microbiology</subject><subject>micrococcal nuclease</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>neutralization</subject><subject>Neutralization Tests</subject><subject>nucleotide sequences</subject><subject>Ovine respiratory syncytial virus</subject><subject>proteins</subject><subject>Replicon</subject><subject>reporter genes</subject><subject>Respiratory syncytial virus</subject><subject>Respiratory Syncytial Virus, Bovine - genetics</subject><subject>Respiratory Syncytial Virus, Bovine - physiology</subject><subject>Respiratory Syncytial Virus, Human - genetics</subject><subject>Respiratory Syncytial Viruses - genetics</subject><subject>Respirovirus</subject><subject>RNA</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>sheep</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>Virology</subject><subject>Virus Replication</subject><subject>viruses</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk1v1DAQhiMEokvhyBUsgTgR8FcS51gqoEgVlbaUa-Q4k9RVYm_tpFJu_SHw53rlTzDprsTHZSXLHs0882o0fpPkOaPvGKXF-4gXzSiegpUPkhWTgqeqKNXDZEUFlanKqTpInsR4RSkmRPY4OWA0y2he0FXyaw3RTEB8SzSp_Y11QALEjQ169GEmcXZmHq3uyY0NUyQdOD9YQ9Zfj4h2uvcdqedd41tyOQ3aYb4he5UgEuNda8MQyXgJ5O72Rzs5M1qPqsS6Ebpgx_nu9ue9HpZN8DGmAYzvnF24pYZzf1iffyfGxlRjt-sI9DCAG-My2MlSW_rPMHiaPGp1H-HZ7j1MLj59_HZ8kp6eff5yfHSaGtzdmILgUtWNZI2grZGqyJTIDcsbrWWtwShoKOSi5IwrwdpC5JnBgAqZF1BrIQ6TN1vdTfDXE8SxGmw00PfagZ9ilZdcckXlXpApKZnI2H6wEKJAOyD46j_wyk8BN4oM5TIvZVHmSKVb6n6lAdpqE-ygw4xQtbiq-sdVyL_YqU71AM1f9NZGCLzeAToa3bdBO_yQPxwrueAcsZdbrNW-0vi_sbo455RJdKYqZM7Fb6425BU</recordid><startdate>19990101</startdate><enddate>19990101</enddate><creator>Yunus, A. 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Psychology</topic><topic>Genetics</topic><topic>Genome, Viral</topic><topic>Helper Viruses - genetics</topic><topic>Helper Viruses - metabolism</topic><topic>Human respiratory syncytial virus</topic><topic>Humans</topic><topic>Microbiology</topic><topic>micrococcal nuclease</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>neutralization</topic><topic>Neutralization Tests</topic><topic>nucleotide sequences</topic><topic>Ovine respiratory syncytial virus</topic><topic>proteins</topic><topic>Replicon</topic><topic>reporter genes</topic><topic>Respiratory syncytial virus</topic><topic>Respiratory Syncytial Virus, Bovine - genetics</topic><topic>Respiratory Syncytial Virus, Bovine - physiology</topic><topic>Respiratory Syncytial Virus, Human - genetics</topic><topic>Respiratory Syncytial Viruses - genetics</topic><topic>Respirovirus</topic><topic>RNA</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>sheep</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>Virology</topic><topic>Virus Replication</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yunus, A. 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S</au><au>Krishnamurthy, S</au><au>Pastey, M. K</au><au>Huang, Z</au><au>Khattar, S. K</au><au>Collins, P. L</au><au>Samal, S. K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rescue of a bovine respiratory syncytial virus genomic RNA analog by bovine, human and ovine respiratory syncytial viruses confirms the “functional integrity” and “cross-recognition” of BRSV cis-acting elements by HRSV and ORSV</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>144</volume><issue>10</issue><spage>1977</spage><epage>1990</epage><pages>1977-1990</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>The nucleotide sequences of the 3′ leader and 5′ trailer regions were determined for genomic RNA of bovine respiratory syncytial virus (BRSV) strain A-51908. The leader and trailer sequences are ‘45’ and ‘161’ nucleotides in length, respectively. The functionality of BRSV leader and trailer sequences and their recognition by HRSV and ovine respiratory syncytial virus (ORSV) proteins were examined with a in vitro transcribed BRSV genomic RNA analog carrying the bacterial chloramphenicol acetyl transferase (CAT) gene under the control of BRSV transcription signals. Upon transfection into BRSV, HRSV or ORSV infected cells, the BRSV minireplicons were ‘rescued’ such that the reporter gene was expressed, the minigenome was replicated and packaged into micrococcal nuclease resistant-infectious minireplicons. The passage of infectious minireplicons could be blocked by a polyclonal BRSV neutralizing antiserum. Bovine parainfluenza virus-3, a heterologous paramyxovirus was inactive in rescuing BRSV genomic RNA analog. Mutational substitution of the G residue at position 4 of leader sequence in the BRSV genomic RNA analog, with an A or U residue inhibited its transcription and replication, while replacement with a C residue had no significant effect on rescue. These results show that the cis-acting elements of BRSV are functional and are also recognized by the proteins of HRSV and ORSV. The helper virus complemented rescue system developed here will be useful for characterizing the cis-acting elements of BRSV.</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer-Verlag</pub><pmid>10550670</pmid><doi>10.1007/s007050050719</doi><tpages>14</tpages></addata></record> |
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subjects | 3' Untranslated Regions - genetics 5' Untranslated Regions - genetics Animals antiserum Biological and medical sciences Blotting, Northern Bovine respiratory syncytial virus Cattle chloramphenicol acetyltransferase Chloramphenicol O-Acetyltransferase - genetics Chloramphenicol O-Acetyltransferase - metabolism Cloning, Molecular DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Genetics Genome, Viral Helper Viruses - genetics Helper Viruses - metabolism Human respiratory syncytial virus Humans Microbiology micrococcal nuclease Molecular Sequence Data Mutation neutralization Neutralization Tests nucleotide sequences Ovine respiratory syncytial virus proteins Replicon reporter genes Respiratory syncytial virus Respiratory Syncytial Virus, Bovine - genetics Respiratory Syncytial Virus, Bovine - physiology Respiratory Syncytial Virus, Human - genetics Respiratory Syncytial Viruses - genetics Respirovirus RNA RNA, Viral - genetics RNA, Viral - isolation & purification sheep Transcription, Genetic Transfection Virology Virus Replication viruses |
title | Rescue of a bovine respiratory syncytial virus genomic RNA analog by bovine, human and ovine respiratory syncytial viruses confirms the “functional integrity” and “cross-recognition” of BRSV cis-acting elements by HRSV and ORSV |
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