Development of multiplex real-time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16

Abstract Large outbreaks of hand, foot, and mouth disease have been reported in the Asia Pacific region over the last few years and resulted in significant fatalities. The 2 main etiologic agents are Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16). Both viruses are closely related genetically an...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2008-07, Vol.61 (3), p.294-301
Hauptverfasser:	Tan, Eng Lee, Tak Kwong Chow, Vincent, Quak, Seng Hock, Yeo, Wei Cheng Andrea, Poh, Chit Laa
Format:	Artikel
Sprache:	eng
Schlagworte:	Coxsackievirus Coxsackievirus A16 Coxsackievirus Infections - diagnosis Coxsackievirus Infections - virology Enterovirus Enterovirus - classification Enterovirus - genetics Enterovirus - isolation & purification Enterovirus 71 Enterovirus Infections - diagnosis Enterovirus Infections - virology Genes, Viral Hand, foot, and mouth disease (HFMD) Humans Infectious Disease Internal Medicine Multiplex real-time RT-PCR Nucleic Acid Hybridization - methods Oligonucleotide Probes - genetics Rapid detection and differentiation Reverse Transcriptase Polymerase Chain Reaction - methods Sensitivity and Specificity
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