Microsatellite Variation within the Human RHCE Gene
Background and Objectives: This study provides a unique method for identifying individuals carrying the Rh haplotype cDe, and supports a model for the evolution of Rh haplotypes in which cDe is the progenitor. Materials and Methods: DNA from 212 unrelated donors of known Rh serological phenotype was...
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| Veröffentlicht in: | Vox sanguinis 1999-01, Vol.77 (3), p.159-163 |
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| container_title | Vox sanguinis |
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| creator | Kemp, Tim J. Poulter, Mark Carritt, Ben |
| description | Background and Objectives:
This study provides a unique method for identifying individuals carrying the Rh haplotype cDe, and supports a model for the evolution of Rh haplotypes in which cDe is the progenitor. Materials and Methods: DNA from 212 unrelated donors of known Rh serological phenotype was PCR amplified. The resulting products were analysed by denaturing polyacrylamide gel electrophoresis, denaturing gradient gel electrophoresis and DNA sequencing. Results: Two adjacent microsatellite repeat elements of the form (AC)n (GCAC)n were found within the human Rh blood group genes. These display copy number variation which was non‐randomly distributed with respect to Rh serological phenotype, and was restricted to alleles of RHCE expressing the c antigen. Conclusion: The predominantly Black African allele cDe displayed a unique set of microsatellite alleles, providing a method of identifying individuals carrying this haplotype. |
| doi_str_mv | 10.1046/j.1423-0410.1999.7730159.x |
| format | Article |
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This study provides a unique method for identifying individuals carrying the Rh haplotype cDe, and supports a model for the evolution of Rh haplotypes in which cDe is the progenitor. Materials and Methods: DNA from 212 unrelated donors of known Rh serological phenotype was PCR amplified. The resulting products were analysed by denaturing polyacrylamide gel electrophoresis, denaturing gradient gel electrophoresis and DNA sequencing. Results: Two adjacent microsatellite repeat elements of the form (AC)n (GCAC)n were found within the human Rh blood group genes. These display copy number variation which was non‐randomly distributed with respect to Rh serological phenotype, and was restricted to alleles of RHCE expressing the c antigen. Conclusion: The predominantly Black African allele cDe displayed a unique set of microsatellite alleles, providing a method of identifying individuals carrying this haplotype.</description><identifier>ISSN: 0042-9007</identifier><identifier>EISSN: 1423-0410</identifier><identifier>DOI: 10.1046/j.1423-0410.1999.7730159.x</identifier><identifier>PMID: 10545853</identifier><identifier>CODEN: VOSAAD</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Africa - ethnology ; Alleles ; Biological and medical sciences ; England - epidemiology ; Europe - ethnology ; Family Health ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glycoproteins - blood ; Glycoproteins - genetics ; Haplotypes ; Humans ; Immunohematology ; Introns ; Japan - ethnology ; Microsatellite Repeats - immunology ; Oncogene Proteins, Fusion - blood ; Polymorphism, Genetic ; Recombinant Fusion Proteins ; Red blood cell immunology ; Rh-Hr Blood-Group System ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid</subject><ispartof>Vox sanguinis, 1999-01, Vol.77 (3), p.159-163</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright S. Karger AG Oct 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4999-950c464b1ee700f78590d5f1bbd1ef3c57ebe726f150ce0da855e38c6ed39e283</citedby><cites>FETCH-LOGICAL-c4999-950c464b1ee700f78590d5f1bbd1ef3c57ebe726f150ce0da855e38c6ed39e283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1423-0410.1999.7730159.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1423-0410.1999.7730159.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1993445$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10545853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kemp, Tim J.</creatorcontrib><creatorcontrib>Poulter, Mark</creatorcontrib><creatorcontrib>Carritt, Ben</creatorcontrib><title>Microsatellite Variation within the Human RHCE Gene</title><title>Vox sanguinis</title><addtitle>Vox Sang</addtitle><description>Background and Objectives:
This study provides a unique method for identifying individuals carrying the Rh haplotype cDe, and supports a model for the evolution of Rh haplotypes in which cDe is the progenitor. Materials and Methods: DNA from 212 unrelated donors of known Rh serological phenotype was PCR amplified. The resulting products were analysed by denaturing polyacrylamide gel electrophoresis, denaturing gradient gel electrophoresis and DNA sequencing. Results: Two adjacent microsatellite repeat elements of the form (AC)n (GCAC)n were found within the human Rh blood group genes. These display copy number variation which was non‐randomly distributed with respect to Rh serological phenotype, and was restricted to alleles of RHCE expressing the c antigen. Conclusion: The predominantly Black African allele cDe displayed a unique set of microsatellite alleles, providing a method of identifying individuals carrying this haplotype.</description><subject>Africa - ethnology</subject><subject>Alleles</subject><subject>Biological and medical sciences</subject><subject>England - epidemiology</subject><subject>Europe - ethnology</subject><subject>Family Health</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glycoproteins - blood</subject><subject>Glycoproteins - genetics</subject><subject>Haplotypes</subject><subject>Humans</subject><subject>Immunohematology</subject><subject>Introns</subject><subject>Japan - ethnology</subject><subject>Microsatellite Repeats - immunology</subject><subject>Oncogene Proteins, Fusion - blood</subject><subject>Polymorphism, Genetic</subject><subject>Recombinant Fusion Proteins</subject><subject>Red blood cell immunology</subject><subject>Rh-Hr Blood-Group System</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0042-9007</issn><issn>1423-0410</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkN1u1DAQhS0EokvhFVBUIe6yjGM7trmDVXcXKFTip3A3cpKJ6iWblDhRt2-Po6wK4g7J0sgz3xwfH8bOOCw5yPzVbsllJlKQU8Nau9RaAFd2eXjAFvejh2wBILPUAugT9iSEHQCYzKjH7ISDksoosWDioy_7LriBmsYPlFy53rvBd21y64dr3ybDNSXbce_a5PN2dZ5sqKWn7FHtmkDPjvWUfVuff11t04vLzbvVm4u0lNFVahWUMpcFJ9IAtTbKQqVqXhQVp1qUSlNBOstrHkGCyhmlSJgyp0pYyow4ZS9n3Zu--zVSGHDvQxmNupa6MWBuMyGkshE8-wfcdWPfRm-YZUKa3GgRodczNP039FTjTe_3rr9DDjjlijucwsMpPJxyxWOueIjLz48vjMWeqr9W5yAj8OIIuFC6pu5dW_rwh7NWSKkitp6xW9_Q3X84wKvLH_HEO06NKJTOQj4MdLgXcv1PzLXQCr9_2uAHs31rv8j3uBa_ARn6ors</recordid><startdate>19990101</startdate><enddate>19990101</enddate><creator>Kemp, Tim J.</creator><creator>Poulter, Mark</creator><creator>Carritt, Ben</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>S. 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Psychology</topic><topic>Fundamental immunology</topic><topic>Glycoproteins - blood</topic><topic>Glycoproteins - genetics</topic><topic>Haplotypes</topic><topic>Humans</topic><topic>Immunohematology</topic><topic>Introns</topic><topic>Japan - ethnology</topic><topic>Microsatellite Repeats - immunology</topic><topic>Oncogene Proteins, Fusion - blood</topic><topic>Polymorphism, Genetic</topic><topic>Recombinant Fusion Proteins</topic><topic>Red blood cell immunology</topic><topic>Rh-Hr Blood-Group System</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kemp, Tim J.</creatorcontrib><creatorcontrib>Poulter, Mark</creatorcontrib><creatorcontrib>Carritt, Ben</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Vox sanguinis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kemp, Tim J.</au><au>Poulter, Mark</au><au>Carritt, Ben</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microsatellite Variation within the Human RHCE Gene</atitle><jtitle>Vox sanguinis</jtitle><addtitle>Vox Sang</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>77</volume><issue>3</issue><spage>159</spage><epage>163</epage><pages>159-163</pages><issn>0042-9007</issn><eissn>1423-0410</eissn><coden>VOSAAD</coden><abstract>Background and Objectives:
This study provides a unique method for identifying individuals carrying the Rh haplotype cDe, and supports a model for the evolution of Rh haplotypes in which cDe is the progenitor. Materials and Methods: DNA from 212 unrelated donors of known Rh serological phenotype was PCR amplified. The resulting products were analysed by denaturing polyacrylamide gel electrophoresis, denaturing gradient gel electrophoresis and DNA sequencing. Results: Two adjacent microsatellite repeat elements of the form (AC)n (GCAC)n were found within the human Rh blood group genes. These display copy number variation which was non‐randomly distributed with respect to Rh serological phenotype, and was restricted to alleles of RHCE expressing the c antigen. Conclusion: The predominantly Black African allele cDe displayed a unique set of microsatellite alleles, providing a method of identifying individuals carrying this haplotype.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>10545853</pmid><doi>10.1046/j.1423-0410.1999.7730159.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | Vox sanguinis, 1999-01, Vol.77 (3), p.159-163 |
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| language | eng |
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| source | MEDLINE; Karger Journals Complete; Wiley Online Library Journals Frontfile Complete |
| subjects | Africa - ethnology Alleles Biological and medical sciences England - epidemiology Europe - ethnology Family Health Fundamental and applied biological sciences. Psychology Fundamental immunology Glycoproteins - blood Glycoproteins - genetics Haplotypes Humans Immunohematology Introns Japan - ethnology Microsatellite Repeats - immunology Oncogene Proteins, Fusion - blood Polymorphism, Genetic Recombinant Fusion Proteins Red blood cell immunology Rh-Hr Blood-Group System Sequence Analysis, DNA Sequence Homology, Nucleic Acid |
| title | Microsatellite Variation within the Human RHCE Gene |
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