Development of a rapid diagnostic assay for methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus
Abstract We describe here a rapid assay for the detection of the tuf gene for the identification of Staphylococcus genus, the femB gene for the identification of Staphylococcus aureus species, and the mecA gene for the identification of methicillin resistance directly from BACTEC blood culture bottl...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2008-07, Vol.61 (3), p.273-279 |
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| container_title | Diagnostic microbiology and infectious disease |
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| creator | Lindsey, Wesley C Woodruff, Evelyn S Weed, Diane Ward, David C Jenison, Robert D |
| description | Abstract We describe here a rapid assay for the detection of the tuf gene for the identification of Staphylococcus genus, the femB gene for the identification of Staphylococcus aureus species, and the mecA gene for the identification of methicillin resistance directly from BACTEC blood culture bottles showing Gram-positive cocci in clusters. The test, configured on a thin-film biosensor platform, allows for detection of genomic DNA from blood culture samples without the need for nucleic acid amplification. In an initial study to validate the technology, 107 consecutive positive blood cultures were tested on the thin-film biosensor, and the assay exhibited 100% concordance in comparison with standard microbiological methods for identifying methicillin-susceptible and methicillin-resistant S. aureus and for identifying methicillin-susceptible and methicillin-resistant coagulase-negative Staphylococcus . Results were obtained within 90 min directly from signal positive bottles with no instrumentation required. |
| doi_str_mv | 10.1016/j.diagmicrobio.2008.03.002 |
| format | Article |
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The test, configured on a thin-film biosensor platform, allows for detection of genomic DNA from blood culture samples without the need for nucleic acid amplification. In an initial study to validate the technology, 107 consecutive positive blood cultures were tested on the thin-film biosensor, and the assay exhibited 100% concordance in comparison with standard microbiological methods for identifying methicillin-susceptible and methicillin-resistant S. aureus and for identifying methicillin-susceptible and methicillin-resistant coagulase-negative Staphylococcus . 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Psychology ; Humans ; Infectious Disease ; Infectious diseases ; Internal Medicine ; mecA ; Medical sciences ; Methicillin Resistance ; Microbiology ; Miscellaneous ; MRSA ; MSSA ; Nucleic Acid Hybridization - methods ; Penicillin-Binding Proteins ; Peptide Elongation Factor Tu - genetics ; Sensitivity and Specificity ; Staphylococcal Infections - microbiology ; Staphylococcus - drug effects ; Staphylococcus - genetics ; Staphylococcus - isolation & purification ; Staphylococcus aureus ; Thin-film biosensor chip</subject><ispartof>Diagnostic microbiology and infectious disease, 2008-07, Vol.61 (3), p.273-279</ispartof><rights>Elsevier Inc.</rights><rights>2008 Elsevier Inc.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-dffcfcf6a24a5a6765af30c9284e744c107f6c81aff4f186bc1728fb0da13d123</citedby><cites>FETCH-LOGICAL-c494t-dffcfcf6a24a5a6765af30c9284e744c107f6c81aff4f186bc1728fb0da13d123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.diagmicrobio.2008.03.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20519764$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18440752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lindsey, Wesley C</creatorcontrib><creatorcontrib>Woodruff, Evelyn S</creatorcontrib><creatorcontrib>Weed, Diane</creatorcontrib><creatorcontrib>Ward, David C</creatorcontrib><creatorcontrib>Jenison, Robert D</creatorcontrib><title>Development of a rapid diagnostic assay for methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract We describe here a rapid assay for the detection of the tuf gene for the identification of Staphylococcus genus, the femB gene for the identification of Staphylococcus aureus species, and the mecA gene for the identification of methicillin resistance directly from BACTEC blood culture bottles showing Gram-positive cocci in clusters. The test, configured on a thin-film biosensor platform, allows for detection of genomic DNA from blood culture samples without the need for nucleic acid amplification. In an initial study to validate the technology, 107 consecutive positive blood cultures were tested on the thin-film biosensor, and the assay exhibited 100% concordance in comparison with standard microbiological methods for identifying methicillin-susceptible and methicillin-resistant S. aureus and for identifying methicillin-susceptible and methicillin-resistant coagulase-negative Staphylococcus . Results were obtained within 90 min directly from signal positive bottles with no instrumentation required.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>CoNS</subject><subject>femB</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>mecA</subject><subject>Medical sciences</subject><subject>Methicillin Resistance</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>MRSA</subject><subject>MSSA</subject><subject>Nucleic Acid Hybridization - methods</subject><subject>Penicillin-Binding Proteins</subject><subject>Peptide Elongation Factor Tu - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus - drug effects</subject><subject>Staphylococcus - genetics</subject><subject>Staphylococcus - isolation & purification</subject><subject>Staphylococcus aureus</subject><subject>Thin-film biosensor chip</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2LFDEQhoMo7uzqX5BG0Fu3lY_-8iAsu64KCx5Wz6EmXZnN2N0Zk-6B-R3-YdPMoLIgSg51eeqtIk8x9pJDwYFXb7ZF53AzOBP82vlCADQFyAJAPGIr3tRtDlDDY7aCWoq8aVp5xs5j3AJw0Sp4ys54oxTUpVixH9e0p97vBhqnzNsMs4A712XLhNHHyZkMY8RDZn3IBprunXF978Y8UHRxwtR1N-Hu_tB7442ZY4ZzoKWM3V9443Ez9xgpH2mDk9vTg4hn7InFPtLzU71gX2_ef7n6mN9-_vDp6vI2N6pVU95Za9KrUCgssaqrEq0E04pGUa2U4VDbyjQcrVWWN9Xa8Fo0dg0dctlxIS_Y62PuLvjvM8VJDy4a6nscyc9RV62QUpXqnyBvq1JBuyS-PYJJTYyBrN4FN2A4aA56cae3-k93enGnQerkLjW_OE2Z1wN1v1tPshLw6gRgNNjbgKNx8RcnoORtXS3rXh85Sp-3dxR0NI5GQ50LZCbdefd_-7x7EGOSSJcmf6MDxa2fw5j0aK6j0KDvlmtbjg2adGhlWcuf6AHYqA</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Lindsey, Wesley C</creator><creator>Woodruff, Evelyn S</creator><creator>Weed, Diane</creator><creator>Ward, David C</creator><creator>Jenison, Robert D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20080701</creationdate><title>Development of a rapid diagnostic assay for methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus</title><author>Lindsey, Wesley C ; Woodruff, Evelyn S ; Weed, Diane ; Ward, David C ; Jenison, Robert D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-dffcfcf6a24a5a6765af30c9284e744c107f6c81aff4f186bc1728fb0da13d123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>CoNS</topic><topic>femB</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Internal Medicine</topic><topic>mecA</topic><topic>Medical sciences</topic><topic>Methicillin Resistance</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>MRSA</topic><topic>MSSA</topic><topic>Nucleic Acid Hybridization - methods</topic><topic>Penicillin-Binding Proteins</topic><topic>Peptide Elongation Factor Tu - genetics</topic><topic>Sensitivity and Specificity</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus - drug effects</topic><topic>Staphylococcus - genetics</topic><topic>Staphylococcus - isolation & purification</topic><topic>Staphylococcus aureus</topic><topic>Thin-film biosensor chip</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lindsey, Wesley C</creatorcontrib><creatorcontrib>Woodruff, Evelyn S</creatorcontrib><creatorcontrib>Weed, Diane</creatorcontrib><creatorcontrib>Ward, David C</creatorcontrib><creatorcontrib>Jenison, Robert D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lindsey, Wesley C</au><au>Woodruff, Evelyn S</au><au>Weed, Diane</au><au>Ward, David C</au><au>Jenison, Robert D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a rapid diagnostic assay for methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>61</volume><issue>3</issue><spage>273</spage><epage>279</epage><pages>273-279</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Abstract We describe here a rapid assay for the detection of the tuf gene for the identification of Staphylococcus genus, the femB gene for the identification of Staphylococcus aureus species, and the mecA gene for the identification of methicillin resistance directly from BACTEC blood culture bottles showing Gram-positive cocci in clusters. The test, configured on a thin-film biosensor platform, allows for detection of genomic DNA from blood culture samples without the need for nucleic acid amplification. In an initial study to validate the technology, 107 consecutive positive blood cultures were tested on the thin-film biosensor, and the assay exhibited 100% concordance in comparison with standard microbiological methods for identifying methicillin-susceptible and methicillin-resistant S. aureus and for identifying methicillin-susceptible and methicillin-resistant coagulase-negative Staphylococcus . Results were obtained within 90 min directly from signal positive bottles with no instrumentation required.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>18440752</pmid><doi>10.1016/j.diagmicrobio.2008.03.002</doi><tpages>7</tpages></addata></record> |
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| subjects | Bacterial Proteins - genetics Bacteriology Biological and medical sciences Biosensing Techniques - methods CoNS femB Fundamental and applied biological sciences. Psychology Humans Infectious Disease Infectious diseases Internal Medicine mecA Medical sciences Methicillin Resistance Microbiology Miscellaneous MRSA MSSA Nucleic Acid Hybridization - methods Penicillin-Binding Proteins Peptide Elongation Factor Tu - genetics Sensitivity and Specificity Staphylococcal Infections - microbiology Staphylococcus - drug effects Staphylococcus - genetics Staphylococcus - isolation & purification Staphylococcus aureus Thin-film biosensor chip |
| title | Development of a rapid diagnostic assay for methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus |
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