Single-Cell High-Throughput Screening To Identify Enantioselective Hydrolytic Enzymes
Getting a look in: A high‐throughput screening method has been developed for the identification and isolation of enantioselective hydrolases displayed on cell surfaces (see scheme; E: Esterase, P: Peroxidase). Enantiomeric substrates labeled with two different fluorescent dyes allow real‐time analys...
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| Veröffentlicht in: | Angewandte Chemie (International ed.) 2008-06, Vol.47 (27), p.5085-5088 |
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| container_end_page | 5088 |
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| container_issue | 27 |
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| container_title | Angewandte Chemie (International ed.) |
| container_volume | 47 |
| creator | Becker, Stefan Höbenreich, Horst Vogel, Andreas Knorr, Janina Wilhelm, Susanne Rosenau, Frank Jaeger, Karl-Erich Reetz, Manfred T Kolmar, Harald |
| description | Getting a look in: A high‐throughput screening method has been developed for the identification and isolation of enantioselective hydrolases displayed on cell surfaces (see scheme; E: Esterase, P: Peroxidase). Enantiomeric substrates labeled with two different fluorescent dyes allow real‐time analysis of enantioselectivity by determination of the ratio of green and red single‐cell fluorescence. |
| doi_str_mv | 10.1002/anie.200705236 |
| format | Article |
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Enantiomeric substrates labeled with two different fluorescent dyes allow real‐time analysis of enantioselectivity by determination of the ratio of green and red single‐cell fluorescence.</description><subject>asymmetric catalysis</subject><subject>directed evolution</subject><subject>Directed Molecular Evolution</subject><subject>Drug Evaluation, Preclinical</subject><subject>enzyme catalysis</subject><subject>Escherichia coli - enzymology</subject><subject>Esterases - chemistry</subject><subject>Flow Cytometry</subject><subject>hydrolases</subject><subject>Hydrolysis</subject><subject>kinetic resolution</subject><subject>Models, Biological</subject><subject>Molecular Structure</subject><subject>Peroxidase - chemistry</subject><subject>Stereoisomerism</subject><subject>Surface Properties</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv00AUha0K1BdsWYJX3TnMw_NaVlFIIlVlkYQsR5OZ62TAscOMTTG_vlM5KuxY3aN7v3Oke7LsA0YTjBD5bBoPE4KQQIxQfpFdY0ZwQYWgb5IuKS2EZPgqu4nxe-KlRPwyu8JpRwgS19lm5Zt9DcUU6jpf-P2hWB9C2-8Pp77LVzYANAnI122-dNB0vhryWWOSaCPUYDv_C_LF4EJbD5236fZnOEJ8l72tTB3h_XneZpsvs_V0UTx8nS-n9w-FLQXmhaN0h5WRTDi3c9IoljYl5gSpignLhQKQUlpRsQqVpSTKcYc4Uw4qKzint9ndmHsK7c8eYqePPtr0immg7aPmilBKOErgZARtaGMMUOlT8EcTBo2RfilSvxSpX4tMho_n5H53BPcXPzeXADUCT76G4T9x-v5xOfs3vBi9Pnbw-9Vrwg_NBRVMbx_nmm-38tuUlnqe-E8jX5lWm33wUW9WBGGKkMKIcUKfAbihl-s</recordid><startdate>20080623</startdate><enddate>20080623</enddate><creator>Becker, Stefan</creator><creator>Höbenreich, Horst</creator><creator>Vogel, Andreas</creator><creator>Knorr, Janina</creator><creator>Wilhelm, Susanne</creator><creator>Rosenau, Frank</creator><creator>Jaeger, Karl-Erich</creator><creator>Reetz, Manfred T</creator><creator>Kolmar, Harald</creator><general>Wiley-VCH Verlag</general><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080623</creationdate><title>Single-Cell High-Throughput Screening To Identify Enantioselective Hydrolytic Enzymes</title><author>Becker, Stefan ; 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| ispartof | Angewandte Chemie (International ed.), 2008-06, Vol.47 (27), p.5085-5088 |
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| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | asymmetric catalysis directed evolution Directed Molecular Evolution Drug Evaluation, Preclinical enzyme catalysis Escherichia coli - enzymology Esterases - chemistry Flow Cytometry hydrolases Hydrolysis kinetic resolution Models, Biological Molecular Structure Peroxidase - chemistry Stereoisomerism Surface Properties |
| title | Single-Cell High-Throughput Screening To Identify Enantioselective Hydrolytic Enzymes |
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