Single-Cell High-Throughput Screening To Identify Enantioselective Hydrolytic Enzymes

Single-Cell High-Throughput Screening To Identify Enantioselective Hydrolytic Enzymes

Getting a look in: A high‐throughput screening method has been developed for the identification and isolation of enantioselective hydrolases displayed on cell surfaces (see scheme; E: Esterase, P: Peroxidase). Enantiomeric substrates labeled with two different fluorescent dyes allow real‐time analys...

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Veröffentlicht in:Angewandte Chemie (International ed.) 2008-06, Vol.47 (27), p.5085-5088
Hauptverfasser: Becker, Stefan, Höbenreich, Horst, Vogel, Andreas, Knorr, Janina, Wilhelm, Susanne, Rosenau, Frank, Jaeger, Karl-Erich, Reetz, Manfred T, Kolmar, Harald
Format: Artikel
Sprache:eng
Schlagworte:
asymmetric catalysis
directed evolution
Directed Molecular Evolution
Drug Evaluation, Preclinical
enzyme catalysis
Escherichia coli - enzymology
Esterases - chemistry
Flow Cytometry
hydrolases
Hydrolysis
kinetic resolution
Models, Biological
Molecular Structure
Peroxidase - chemistry
Stereoisomerism
Surface Properties
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Zusammenfassung:Getting a look in: A high‐throughput screening method has been developed for the identification and isolation of enantioselective hydrolases displayed on cell surfaces (see scheme; E: Esterase, P: Peroxidase). Enantiomeric substrates labeled with two different fluorescent dyes allow real‐time analysis of enantioselectivity by determination of the ratio of green and red single‐cell fluorescence.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200705236