Simultaneous Determination of Baicalin, Wogonoside, Baicalein, Wogonin, Berberine, Coptisine, Palmatine, Jateorrhizine and Glycyrrhizin in Kampo Medicines by Ion-Pair High-Performance Liquid Chromatography
An ion-pair high-performance liquid chromatographic method for the simultaneous determination of four flavonoids, namely baicalin, wogonoside, baicalein and wogonin, and four berberine-type alkaloids, namely berberine, coptisine, palmatine and jateorrhizine, and glycyrrhizin in Kampo medicines is de...
Gespeichert in:
| Veröffentlicht in: | Biological & pharmaceutical bulletin 1999/10/15, Vol.22(10), pp.1015-1021 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1021 |
|---|---|
| container_issue | 10 |
| container_start_page | 1015 |
| container_title | Biological & pharmaceutical bulletin |
| container_volume | 22 |
| creator | OKAMURA, Nobuyuki MIKI, Hirotsugu ISHIDA, Satie ONO, Hiroko YANO, Akiko TANAKA, Takashi ONO, Yukio YAGI, Akira |
| description | An ion-pair high-performance liquid chromatographic method for the simultaneous determination of four flavonoids, namely baicalin, wogonoside, baicalein and wogonin, and four berberine-type alkaloids, namely berberine, coptisine, palmatine and jateorrhizine, and glycyrrhizin in Kampo medicines is described. The analysis can be accomplished within 30 min with a Wakosil-II 5C18 HG column by linear gradient elution using a mobile phase containing aqueous phosphoric acid, sodium dodecyl sulfate and acetonitrile at a flow-rate of 1.0 ml·min-1, a thermostatic oven at 45°C, and detection at 265nm. The method was applied to quantifying these components in three Kampo decoctions : Oren-gedoku-to, San'o-shashin-to and Hange-shashin-to. The decoctions were diluted with 65% methanol at the final stage because a large quantity of precipitate, mainly from baicalin and berberine, was formed. The within-day relative standard deviations were less than 2.02% (n=10). The recoveries of these compounds were 90.3-102%. The detection limits of these compounds were 0.02-1.96 μM per injection (5 μl). |
| doi_str_mv | 10.1248/bpb.22.1015 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69232642</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69232642</sourcerecordid><originalsourceid>FETCH-LOGICAL-c634t-7e50398acb5af1b3323c5d01fc86a6cee1581757389bc8a70e6aa268620164d53</originalsourceid><addsrcrecordid>eNpdkU2P0zAQhiMEYpeFE3dkCcSFTfFH4jhHtrAfUEQlQByjiTNpXSVx1k4O5T_yn3BIVBCSZb-eeTQzmjeKnjO6YjxRb8u-XHG-YpSlD6JzJpIsTjlLH0bnNGcqlixVZ9ET7w-U0oxy8Tg6YzRNcpWo8-jXV9OOzQAd2tGT9ziga00Hg7EdsTW5AqOhMd0l-WF3trPeVHi5RPEUnsQVuhKd6UJ6bfvB-D9yC00bik3yIwxondubn-FLoKvITXPUxyVCwvkEbW_JZ6yMDogn5ZHc2S7egnHk1uz28RZdbV0LnUayMfejqch672zoYHcO-v3xafSohsbjs-W9iL5ff_i2vo03X27u1u82sZYiGeIMUypyBbpMoWalEFzotKKs1kqC1IhhZSxLM6HyUivIKEoALpXklMmkSsVF9Hqu2zt7P6IfitZ4jU0z77GQORdcJjyAL_8DD3Z0XZitYEkS7OFZLgL1Zqa0s947rIvemRbcsWC0mDwugscF58XkcaBfLDXHssXqH3Y2NQCvFgB88Kl2YWHG_-VYriSjAbuesYMfYIenPLjB6AannizPxdx3uacBToDegyuwE78BtQnJ0A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1449182793</pqid></control><display><type>article</type><title>Simultaneous Determination of Baicalin, Wogonoside, Baicalein, Wogonin, Berberine, Coptisine, Palmatine, Jateorrhizine and Glycyrrhizin in Kampo Medicines by Ion-Pair High-Performance Liquid Chromatography</title><source>J-STAGE Free</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>OKAMURA, Nobuyuki ; MIKI, Hirotsugu ; ISHIDA, Satie ; ONO, Hiroko ; YANO, Akiko ; TANAKA, Takashi ; ONO, Yukio ; YAGI, Akira</creator><creatorcontrib>OKAMURA, Nobuyuki ; MIKI, Hirotsugu ; ISHIDA, Satie ; ONO, Hiroko ; YANO, Akiko ; TANAKA, Takashi ; ONO, Yukio ; YAGI, Akira</creatorcontrib><description>An ion-pair high-performance liquid chromatographic method for the simultaneous determination of four flavonoids, namely baicalin, wogonoside, baicalein and wogonin, and four berberine-type alkaloids, namely berberine, coptisine, palmatine and jateorrhizine, and glycyrrhizin in Kampo medicines is described. The analysis can be accomplished within 30 min with a Wakosil-II 5C18 HG column by linear gradient elution using a mobile phase containing aqueous phosphoric acid, sodium dodecyl sulfate and acetonitrile at a flow-rate of 1.0 ml·min-1, a thermostatic oven at 45°C, and detection at 265nm. The method was applied to quantifying these components in three Kampo decoctions : Oren-gedoku-to, San'o-shashin-to and Hange-shashin-to. The decoctions were diluted with 65% methanol at the final stage because a large quantity of precipitate, mainly from baicalin and berberine, was formed. The within-day relative standard deviations were less than 2.02% (n=10). The recoveries of these compounds were 90.3-102%. The detection limits of these compounds were 0.02-1.96 μM per injection (5 μl).</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.22.1015</identifier><identifier>PMID: 10549848</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Alkaloids - analysis ; Analysis ; baicalin ; berberine ; Berberine - analogs & derivatives ; Berberine - analysis ; Berberine Alkaloids - analysis ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Flavanones ; Flavonoids - analysis ; General pharmacology ; Glycyrrhizic Acid - analysis ; Hange-shashin-to ; ion-pair HPLC ; Medical sciences ; Medicine, Kampo ; Oren-gedoku-to ; Pharmacology. Drug treatments ; San'o-shashin-to</subject><ispartof>Biological and Pharmaceutical Bulletin, 1999/10/15, Vol.22(10), pp.1015-1021</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>2000 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c634t-7e50398acb5af1b3323c5d01fc86a6cee1581757389bc8a70e6aa268620164d53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1198610$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10549848$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OKAMURA, Nobuyuki</creatorcontrib><creatorcontrib>MIKI, Hirotsugu</creatorcontrib><creatorcontrib>ISHIDA, Satie</creatorcontrib><creatorcontrib>ONO, Hiroko</creatorcontrib><creatorcontrib>YANO, Akiko</creatorcontrib><creatorcontrib>TANAKA, Takashi</creatorcontrib><creatorcontrib>ONO, Yukio</creatorcontrib><creatorcontrib>YAGI, Akira</creatorcontrib><title>Simultaneous Determination of Baicalin, Wogonoside, Baicalein, Wogonin, Berberine, Coptisine, Palmatine, Jateorrhizine and Glycyrrhizin in Kampo Medicines by Ion-Pair High-Performance Liquid Chromatography</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>An ion-pair high-performance liquid chromatographic method for the simultaneous determination of four flavonoids, namely baicalin, wogonoside, baicalein and wogonin, and four berberine-type alkaloids, namely berberine, coptisine, palmatine and jateorrhizine, and glycyrrhizin in Kampo medicines is described. The analysis can be accomplished within 30 min with a Wakosil-II 5C18 HG column by linear gradient elution using a mobile phase containing aqueous phosphoric acid, sodium dodecyl sulfate and acetonitrile at a flow-rate of 1.0 ml·min-1, a thermostatic oven at 45°C, and detection at 265nm. The method was applied to quantifying these components in three Kampo decoctions : Oren-gedoku-to, San'o-shashin-to and Hange-shashin-to. The decoctions were diluted with 65% methanol at the final stage because a large quantity of precipitate, mainly from baicalin and berberine, was formed. The within-day relative standard deviations were less than 2.02% (n=10). The recoveries of these compounds were 90.3-102%. The detection limits of these compounds were 0.02-1.96 μM per injection (5 μl).</description><subject>Alkaloids - analysis</subject><subject>Analysis</subject><subject>baicalin</subject><subject>berberine</subject><subject>Berberine - analogs & derivatives</subject><subject>Berberine - analysis</subject><subject>Berberine Alkaloids - analysis</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Flavanones</subject><subject>Flavonoids - analysis</subject><subject>General pharmacology</subject><subject>Glycyrrhizic Acid - analysis</subject><subject>Hange-shashin-to</subject><subject>ion-pair HPLC</subject><subject>Medical sciences</subject><subject>Medicine, Kampo</subject><subject>Oren-gedoku-to</subject><subject>Pharmacology. Drug treatments</subject><subject>San'o-shashin-to</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU2P0zAQhiMEYpeFE3dkCcSFTfFH4jhHtrAfUEQlQByjiTNpXSVx1k4O5T_yn3BIVBCSZb-eeTQzmjeKnjO6YjxRb8u-XHG-YpSlD6JzJpIsTjlLH0bnNGcqlixVZ9ET7w-U0oxy8Tg6YzRNcpWo8-jXV9OOzQAd2tGT9ziga00Hg7EdsTW5AqOhMd0l-WF3trPeVHi5RPEUnsQVuhKd6UJ6bfvB-D9yC00bik3yIwxondubn-FLoKvITXPUxyVCwvkEbW_JZ6yMDogn5ZHc2S7egnHk1uz28RZdbV0LnUayMfejqch672zoYHcO-v3xafSohsbjs-W9iL5ff_i2vo03X27u1u82sZYiGeIMUypyBbpMoWalEFzotKKs1kqC1IhhZSxLM6HyUivIKEoALpXklMmkSsVF9Hqu2zt7P6IfitZ4jU0z77GQORdcJjyAL_8DD3Z0XZitYEkS7OFZLgL1Zqa0s947rIvemRbcsWC0mDwugscF58XkcaBfLDXHssXqH3Y2NQCvFgB88Kl2YWHG_-VYriSjAbuesYMfYIenPLjB6AannizPxdx3uacBToDegyuwE78BtQnJ0A</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>OKAMURA, Nobuyuki</creator><creator>MIKI, Hirotsugu</creator><creator>ISHIDA, Satie</creator><creator>ONO, Hiroko</creator><creator>YANO, Akiko</creator><creator>TANAKA, Takashi</creator><creator>ONO, Yukio</creator><creator>YAGI, Akira</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Simultaneous Determination of Baicalin, Wogonoside, Baicalein, Wogonin, Berberine, Coptisine, Palmatine, Jateorrhizine and Glycyrrhizin in Kampo Medicines by Ion-Pair High-Performance Liquid Chromatography</title><author>OKAMURA, Nobuyuki ; MIKI, Hirotsugu ; ISHIDA, Satie ; ONO, Hiroko ; YANO, Akiko ; TANAKA, Takashi ; ONO, Yukio ; YAGI, Akira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c634t-7e50398acb5af1b3323c5d01fc86a6cee1581757389bc8a70e6aa268620164d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Alkaloids - analysis</topic><topic>Analysis</topic><topic>baicalin</topic><topic>berberine</topic><topic>Berberine - analogs & derivatives</topic><topic>Berberine - analysis</topic><topic>Berberine Alkaloids - analysis</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Flavanones</topic><topic>Flavonoids - analysis</topic><topic>General pharmacology</topic><topic>Glycyrrhizic Acid - analysis</topic><topic>Hange-shashin-to</topic><topic>ion-pair HPLC</topic><topic>Medical sciences</topic><topic>Medicine, Kampo</topic><topic>Oren-gedoku-to</topic><topic>Pharmacology. Drug treatments</topic><topic>San'o-shashin-to</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OKAMURA, Nobuyuki</creatorcontrib><creatorcontrib>MIKI, Hirotsugu</creatorcontrib><creatorcontrib>ISHIDA, Satie</creatorcontrib><creatorcontrib>ONO, Hiroko</creatorcontrib><creatorcontrib>YANO, Akiko</creatorcontrib><creatorcontrib>TANAKA, Takashi</creatorcontrib><creatorcontrib>ONO, Yukio</creatorcontrib><creatorcontrib>YAGI, Akira</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OKAMURA, Nobuyuki</au><au>MIKI, Hirotsugu</au><au>ISHIDA, Satie</au><au>ONO, Hiroko</au><au>YANO, Akiko</au><au>TANAKA, Takashi</au><au>ONO, Yukio</au><au>YAGI, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous Determination of Baicalin, Wogonoside, Baicalein, Wogonin, Berberine, Coptisine, Palmatine, Jateorrhizine and Glycyrrhizin in Kampo Medicines by Ion-Pair High-Performance Liquid Chromatography</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>22</volume><issue>10</issue><spage>1015</spage><epage>1021</epage><pages>1015-1021</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>An ion-pair high-performance liquid chromatographic method for the simultaneous determination of four flavonoids, namely baicalin, wogonoside, baicalein and wogonin, and four berberine-type alkaloids, namely berberine, coptisine, palmatine and jateorrhizine, and glycyrrhizin in Kampo medicines is described. The analysis can be accomplished within 30 min with a Wakosil-II 5C18 HG column by linear gradient elution using a mobile phase containing aqueous phosphoric acid, sodium dodecyl sulfate and acetonitrile at a flow-rate of 1.0 ml·min-1, a thermostatic oven at 45°C, and detection at 265nm. The method was applied to quantifying these components in three Kampo decoctions : Oren-gedoku-to, San'o-shashin-to and Hange-shashin-to. The decoctions were diluted with 65% methanol at the final stage because a large quantity of precipitate, mainly from baicalin and berberine, was formed. The within-day relative standard deviations were less than 2.02% (n=10). The recoveries of these compounds were 90.3-102%. The detection limits of these compounds were 0.02-1.96 μM per injection (5 μl).</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>10549848</pmid><doi>10.1248/bpb.22.1015</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0918-6158 |
| ispartof | Biological and Pharmaceutical Bulletin, 1999/10/15, Vol.22(10), pp.1015-1021 |
| issn | 0918-6158 1347-5215 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69232642 |
| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Alkaloids - analysis Analysis baicalin berberine Berberine - analogs & derivatives Berberine - analysis Berberine Alkaloids - analysis Biological and medical sciences Chromatography, High Pressure Liquid - methods Flavanones Flavonoids - analysis General pharmacology Glycyrrhizic Acid - analysis Hange-shashin-to ion-pair HPLC Medical sciences Medicine, Kampo Oren-gedoku-to Pharmacology. Drug treatments San'o-shashin-to |
| title | Simultaneous Determination of Baicalin, Wogonoside, Baicalein, Wogonin, Berberine, Coptisine, Palmatine, Jateorrhizine and Glycyrrhizin in Kampo Medicines by Ion-Pair High-Performance Liquid Chromatography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T23%3A04%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20Determination%20of%20Baicalin,%20Wogonoside,%20Baicalein,%20Wogonin,%20Berberine,%20Coptisine,%20Palmatine,%20Jateorrhizine%20and%20Glycyrrhizin%20in%20Kampo%20Medicines%20by%20Ion-Pair%20High-Performance%20Liquid%20Chromatography&rft.jtitle=Biological%20&%20pharmaceutical%20bulletin&rft.au=OKAMURA,%20Nobuyuki&rft.date=1999-10-01&rft.volume=22&rft.issue=10&rft.spage=1015&rft.epage=1021&rft.pages=1015-1021&rft.issn=0918-6158&rft.eissn=1347-5215&rft_id=info:doi/10.1248/bpb.22.1015&rft_dat=%3Cproquest_cross%3E69232642%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1449182793&rft_id=info:pmid/10549848&rfr_iscdi=true |