Differential Roles of TLR2 and TLR4 in Recognition of Gram-Negative and Gram-Positive Bacterial Cell Wall Components
Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice r...
Gespeichert in:
| Veröffentlicht in: | Immunity (Cambridge, Mass.) Mass.), 1999-10, Vol.11 (4), p.443-451 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 451 |
|---|---|
| container_issue | 4 |
| container_start_page | 443 |
| container_title | Immunity (Cambridge, Mass.) |
| container_volume | 11 |
| creator | Takeuchi, Osamu Hoshino, Katsuaki Kawai, Taro Sanjo, Hideki Takada, Haruhiko Ogawa, Tomohiko Takeda, Kiyoshi Akira, Shizuo |
| description | Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as
Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition. |
| doi_str_mv | 10.1016/S1074-7613(00)80119-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69232628</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1074761300801193</els_id><sourcerecordid>17435103</sourcerecordid><originalsourceid>FETCH-LOGICAL-c557t-43484edf24790d715970ac176321c87d46d9219bcec625f7bbea9d9d39e66ad73</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhiMEol_8BFBOiB5CZ2zHjk8IFiiVVlAtRRwtrz2pjJJ4a2cr8e9Jsnvg1svMaPzMvNa8RfEa4T0CyqufCEpUSiJ_B3DZAKKu-LPiFEGrSmADz-f6iJwUZzn_AUBRa3hZnCDUQksmT4vxc2hbSjSMwXblJnaUy9iWd-sNK-3g50KUYSg35OL9EMYQh_n9Otm--k73dgyPtIBL5zbmsHQ-WTdSmleuqOvK33YKq9jv4jAp5YviRWu7TK-O-bz49fXL3epbtf5xfbP6uK5cXauxElw0gnzLhNLgFdZagXWoJGfoGuWF9Jqh3jpyktWt2m7Jaq891ySl9YqfF28Pe3cpPuwpj6YP2U0fsgPFfTZSM84ka54EUQleI_AJrA-gSzHnRK3ZpdDb9NcgmNkXs_hi5qMbALP4Yua5N0eB_bYn_9_UwYgJ-HAAaLrHY6Bksgs0OPIhkRuNj-EJiX-lWpu8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17435103</pqid></control><display><type>article</type><title>Differential Roles of TLR2 and TLR4 in Recognition of Gram-Negative and Gram-Positive Bacterial Cell Wall Components</title><source>MEDLINE</source><source>Cell Press Free Archives</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Takeuchi, Osamu ; Hoshino, Katsuaki ; Kawai, Taro ; Sanjo, Hideki ; Takada, Haruhiko ; Ogawa, Tomohiko ; Takeda, Kiyoshi ; Akira, Shizuo</creator><creatorcontrib>Takeuchi, Osamu ; Hoshino, Katsuaki ; Kawai, Taro ; Sanjo, Hideki ; Takada, Haruhiko ; Ogawa, Tomohiko ; Takeda, Kiyoshi ; Akira, Shizuo</creatorcontrib><description>Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as
Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.</description><identifier>ISSN: 1074-7613</identifier><identifier>EISSN: 1097-4180</identifier><identifier>DOI: 10.1016/S1074-7613(00)80119-3</identifier><identifier>PMID: 10549626</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antigens, Bacterial - immunology ; Cell Wall - immunology ; Corynebacterium diphtheriae - chemistry ; Corynebacterium diphtheriae - immunology ; Drosophila Proteins ; Escherichia coli - chemistry ; Escherichia coli - immunology ; Female ; Gram-Negative Bacteria - chemistry ; Gram-Negative Bacteria - immunology ; Gram-Positive Bacteria - chemistry ; Gram-Positive Bacteria - immunology ; Interleukin-1 Receptor-Associated Kinases ; Lipid A - immunology ; lipopolysaccharides ; Lipopolysaccharides - immunology ; Macrophages, Peritoneal - immunology ; Male ; Membrane Glycoproteins - deficiency ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - physiology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; NF-kappa B - metabolism ; Nocardia - chemistry ; Nocardia - immunology ; Peptidoglycan - immunology ; Protein Isoforms - deficiency ; Protein Isoforms - genetics ; Protein Isoforms - physiology ; Protein Kinases - metabolism ; Receptors, Cell Surface - deficiency ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - physiology ; Salmonella - chemistry ; Salmonella - immunology ; Signal Transduction ; Species Specificity ; Staphylococcus aureus ; Staphylococcus aureus - chemistry ; Staphylococcus aureus - immunology ; Teichoic Acids - immunology ; Toll-Like Receptor 2 ; Toll-Like Receptor 4 ; Toll-Like Receptors</subject><ispartof>Immunity (Cambridge, Mass.), 1999-10, Vol.11 (4), p.443-451</ispartof><rights>1999 Cell Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-43484edf24790d715970ac176321c87d46d9219bcec625f7bbea9d9d39e66ad73</citedby><cites>FETCH-LOGICAL-c557t-43484edf24790d715970ac176321c87d46d9219bcec625f7bbea9d9d39e66ad73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1074-7613(00)80119-3$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10549626$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takeuchi, Osamu</creatorcontrib><creatorcontrib>Hoshino, Katsuaki</creatorcontrib><creatorcontrib>Kawai, Taro</creatorcontrib><creatorcontrib>Sanjo, Hideki</creatorcontrib><creatorcontrib>Takada, Haruhiko</creatorcontrib><creatorcontrib>Ogawa, Tomohiko</creatorcontrib><creatorcontrib>Takeda, Kiyoshi</creatorcontrib><creatorcontrib>Akira, Shizuo</creatorcontrib><title>Differential Roles of TLR2 and TLR4 in Recognition of Gram-Negative and Gram-Positive Bacterial Cell Wall Components</title><title>Immunity (Cambridge, Mass.)</title><addtitle>Immunity</addtitle><description>Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as
Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.</description><subject>Animals</subject><subject>Antigens, Bacterial - immunology</subject><subject>Cell Wall - immunology</subject><subject>Corynebacterium diphtheriae - chemistry</subject><subject>Corynebacterium diphtheriae - immunology</subject><subject>Drosophila Proteins</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - immunology</subject><subject>Female</subject><subject>Gram-Negative Bacteria - chemistry</subject><subject>Gram-Negative Bacteria - immunology</subject><subject>Gram-Positive Bacteria - chemistry</subject><subject>Gram-Positive Bacteria - immunology</subject><subject>Interleukin-1 Receptor-Associated Kinases</subject><subject>Lipid A - immunology</subject><subject>lipopolysaccharides</subject><subject>Lipopolysaccharides - immunology</subject><subject>Macrophages, Peritoneal - immunology</subject><subject>Male</subject><subject>Membrane Glycoproteins - deficiency</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>NF-kappa B - metabolism</subject><subject>Nocardia - chemistry</subject><subject>Nocardia - immunology</subject><subject>Peptidoglycan - immunology</subject><subject>Protein Isoforms - deficiency</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - physiology</subject><subject>Protein Kinases - metabolism</subject><subject>Receptors, Cell Surface - deficiency</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - physiology</subject><subject>Salmonella - chemistry</subject><subject>Salmonella - immunology</subject><subject>Signal Transduction</subject><subject>Species Specificity</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - chemistry</subject><subject>Staphylococcus aureus - immunology</subject><subject>Teichoic Acids - immunology</subject><subject>Toll-Like Receptor 2</subject><subject>Toll-Like Receptor 4</subject><subject>Toll-Like Receptors</subject><issn>1074-7613</issn><issn>1097-4180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEol_8BFBOiB5CZ2zHjk8IFiiVVlAtRRwtrz2pjJJ4a2cr8e9Jsnvg1svMaPzMvNa8RfEa4T0CyqufCEpUSiJ_B3DZAKKu-LPiFEGrSmADz-f6iJwUZzn_AUBRa3hZnCDUQksmT4vxc2hbSjSMwXblJnaUy9iWd-sNK-3g50KUYSg35OL9EMYQh_n9Otm--k73dgyPtIBL5zbmsHQ-WTdSmleuqOvK33YKq9jv4jAp5YviRWu7TK-O-bz49fXL3epbtf5xfbP6uK5cXauxElw0gnzLhNLgFdZagXWoJGfoGuWF9Jqh3jpyktWt2m7Jaq891ySl9YqfF28Pe3cpPuwpj6YP2U0fsgPFfTZSM84ka54EUQleI_AJrA-gSzHnRK3ZpdDb9NcgmNkXs_hi5qMbALP4Yua5N0eB_bYn_9_UwYgJ-HAAaLrHY6Bksgs0OPIhkRuNj-EJiX-lWpu8</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Takeuchi, Osamu</creator><creator>Hoshino, Katsuaki</creator><creator>Kawai, Taro</creator><creator>Sanjo, Hideki</creator><creator>Takada, Haruhiko</creator><creator>Ogawa, Tomohiko</creator><creator>Takeda, Kiyoshi</creator><creator>Akira, Shizuo</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Differential Roles of TLR2 and TLR4 in Recognition of Gram-Negative and Gram-Positive Bacterial Cell Wall Components</title><author>Takeuchi, Osamu ; Hoshino, Katsuaki ; Kawai, Taro ; Sanjo, Hideki ; Takada, Haruhiko ; Ogawa, Tomohiko ; Takeda, Kiyoshi ; Akira, Shizuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-43484edf24790d715970ac176321c87d46d9219bcec625f7bbea9d9d39e66ad73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antigens, Bacterial - immunology</topic><topic>Cell Wall - immunology</topic><topic>Corynebacterium diphtheriae - chemistry</topic><topic>Corynebacterium diphtheriae - immunology</topic><topic>Drosophila Proteins</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli - immunology</topic><topic>Female</topic><topic>Gram-Negative Bacteria - chemistry</topic><topic>Gram-Negative Bacteria - immunology</topic><topic>Gram-Positive Bacteria - chemistry</topic><topic>Gram-Positive Bacteria - immunology</topic><topic>Interleukin-1 Receptor-Associated Kinases</topic><topic>Lipid A - immunology</topic><topic>lipopolysaccharides</topic><topic>Lipopolysaccharides - immunology</topic><topic>Macrophages, Peritoneal - immunology</topic><topic>Male</topic><topic>Membrane Glycoproteins - deficiency</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>NF-kappa B - metabolism</topic><topic>Nocardia - chemistry</topic><topic>Nocardia - immunology</topic><topic>Peptidoglycan - immunology</topic><topic>Protein Isoforms - deficiency</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - physiology</topic><topic>Protein Kinases - metabolism</topic><topic>Receptors, Cell Surface - deficiency</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - physiology</topic><topic>Salmonella - chemistry</topic><topic>Salmonella - immunology</topic><topic>Signal Transduction</topic><topic>Species Specificity</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - chemistry</topic><topic>Staphylococcus aureus - immunology</topic><topic>Teichoic Acids - immunology</topic><topic>Toll-Like Receptor 2</topic><topic>Toll-Like Receptor 4</topic><topic>Toll-Like Receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takeuchi, Osamu</creatorcontrib><creatorcontrib>Hoshino, Katsuaki</creatorcontrib><creatorcontrib>Kawai, Taro</creatorcontrib><creatorcontrib>Sanjo, Hideki</creatorcontrib><creatorcontrib>Takada, Haruhiko</creatorcontrib><creatorcontrib>Ogawa, Tomohiko</creatorcontrib><creatorcontrib>Takeda, Kiyoshi</creatorcontrib><creatorcontrib>Akira, Shizuo</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunity (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takeuchi, Osamu</au><au>Hoshino, Katsuaki</au><au>Kawai, Taro</au><au>Sanjo, Hideki</au><au>Takada, Haruhiko</au><au>Ogawa, Tomohiko</au><au>Takeda, Kiyoshi</au><au>Akira, Shizuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Roles of TLR2 and TLR4 in Recognition of Gram-Negative and Gram-Positive Bacterial Cell Wall Components</atitle><jtitle>Immunity (Cambridge, Mass.)</jtitle><addtitle>Immunity</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>11</volume><issue>4</issue><spage>443</spage><epage>451</epage><pages>443-451</pages><issn>1074-7613</issn><eissn>1097-4180</eissn><abstract>Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as
Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10549626</pmid><doi>10.1016/S1074-7613(00)80119-3</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1074-7613 |
| ispartof | Immunity (Cambridge, Mass.), 1999-10, Vol.11 (4), p.443-451 |
| issn | 1074-7613 1097-4180 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69232628 |
| source | MEDLINE; Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antigens, Bacterial - immunology Cell Wall - immunology Corynebacterium diphtheriae - chemistry Corynebacterium diphtheriae - immunology Drosophila Proteins Escherichia coli - chemistry Escherichia coli - immunology Female Gram-Negative Bacteria - chemistry Gram-Negative Bacteria - immunology Gram-Positive Bacteria - chemistry Gram-Positive Bacteria - immunology Interleukin-1 Receptor-Associated Kinases Lipid A - immunology lipopolysaccharides Lipopolysaccharides - immunology Macrophages, Peritoneal - immunology Male Membrane Glycoproteins - deficiency Membrane Glycoproteins - genetics Membrane Glycoproteins - physiology Mice Mice, Inbred C57BL Mice, Knockout NF-kappa B - metabolism Nocardia - chemistry Nocardia - immunology Peptidoglycan - immunology Protein Isoforms - deficiency Protein Isoforms - genetics Protein Isoforms - physiology Protein Kinases - metabolism Receptors, Cell Surface - deficiency Receptors, Cell Surface - genetics Receptors, Cell Surface - physiology Salmonella - chemistry Salmonella - immunology Signal Transduction Species Specificity Staphylococcus aureus Staphylococcus aureus - chemistry Staphylococcus aureus - immunology Teichoic Acids - immunology Toll-Like Receptor 2 Toll-Like Receptor 4 Toll-Like Receptors |
| title | Differential Roles of TLR2 and TLR4 in Recognition of Gram-Negative and Gram-Positive Bacterial Cell Wall Components |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T19%3A20%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20Roles%20of%20TLR2%20and%20TLR4%20in%20Recognition%20of%20Gram-Negative%20and%20Gram-Positive%20Bacterial%20Cell%20Wall%20Components&rft.jtitle=Immunity%20(Cambridge,%20Mass.)&rft.au=Takeuchi,%20Osamu&rft.date=1999-10-01&rft.volume=11&rft.issue=4&rft.spage=443&rft.epage=451&rft.pages=443-451&rft.issn=1074-7613&rft.eissn=1097-4180&rft_id=info:doi/10.1016/S1074-7613(00)80119-3&rft_dat=%3Cproquest_cross%3E17435103%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17435103&rft_id=info:pmid/10549626&rft_els_id=S1074761300801193&rfr_iscdi=true |