Characterization of kinetoplast DNA minicircles of an Indian isolate of Leishmania donovani
Characterization of the kinetoplast DNA minicircles in a human pathogenic Indian isolate of Leishmania donovani has not been reported previously. Using inverse PCR, we constructed a library of PCR-amplified minicircle variable region from the kinetoplast DNA of this isolate. A combination of restric...
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| Veröffentlicht in: | Acta tropica 1999-10, Vol.73 (3), p.313-319 |
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| creator | Singh, Neeloo Curran, Martin D Middleton, Derek Rastogi, Anil K |
| description | Characterization of the kinetoplast DNA minicircles in a human pathogenic Indian isolate of
Leishmania donovani has not been reported previously. Using inverse PCR, we constructed a library of PCR-amplified minicircle variable region from the kinetoplast DNA of this isolate. A combination of restriction enzyme digestion and nucleotide sequence analysis revealed five minicircle DNA sequence classes within the library, one of which was predominant, representing 75% of the kDNA network. Another distinct sequence class represented 15% of the minicircle network. Other minor sequence classes collectively constituted the remaining 10% of the network. Apart from generating basic information on the organisation and distribution of the different sequence classes within the minicircles, the DNA sequence analysis also revealed unique attributes to our minicircles. One was the surprising homology of our isolate (an Old World sp.) with distantly related New World
Leishmania species. Secondly, open reading frames were also identified, indicating the possibility that these minicircles may have more than a structural role to play within the kinetoplast network. |
| doi_str_mv | 10.1016/S0001-706X(99)00036-4 |
| format | Article |
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Leishmania donovani has not been reported previously. Using inverse PCR, we constructed a library of PCR-amplified minicircle variable region from the kinetoplast DNA of this isolate. A combination of restriction enzyme digestion and nucleotide sequence analysis revealed five minicircle DNA sequence classes within the library, one of which was predominant, representing 75% of the kDNA network. Another distinct sequence class represented 15% of the minicircle network. Other minor sequence classes collectively constituted the remaining 10% of the network. Apart from generating basic information on the organisation and distribution of the different sequence classes within the minicircles, the DNA sequence analysis also revealed unique attributes to our minicircles. One was the surprising homology of our isolate (an Old World sp.) with distantly related New World
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Leishmania donovani has not been reported previously. Using inverse PCR, we constructed a library of PCR-amplified minicircle variable region from the kinetoplast DNA of this isolate. A combination of restriction enzyme digestion and nucleotide sequence analysis revealed five minicircle DNA sequence classes within the library, one of which was predominant, representing 75% of the kDNA network. Another distinct sequence class represented 15% of the minicircle network. Other minor sequence classes collectively constituted the remaining 10% of the network. Apart from generating basic information on the organisation and distribution of the different sequence classes within the minicircles, the DNA sequence analysis also revealed unique attributes to our minicircles. One was the surprising homology of our isolate (an Old World sp.) with distantly related New World
Leishmania species. Secondly, open reading frames were also identified, indicating the possibility that these minicircles may have more than a structural role to play within the kinetoplast network.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>DNA sequencing</subject><subject>DNA, Kinetoplast - genetics</subject><subject>Evolution</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Indian</subject><subject>Infectious diseases</subject><subject>Inverse PCR</subject><subject>Kinetoplast DNA</subject><subject>Leishmania donovani</subject><subject>Leishmania donovani - genetics</subject><subject>Leishmania donovani - isolation & purification</subject><subject>Leishmaniasis, Visceral - parasitology</subject><subject>Leshmaniasis</subject><subject>Medical sciences</subject><subject>Minicircles</subject><subject>Molecular Sequence Data</subject><subject>Parasitic diseases</subject><subject>Pathogenic</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Protozoal diseases</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Tropical medicine</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtvEzEQgC1ERUPKTwDtASF6WPBr_TihKlBaKYJDQULiYDn2WDXs2qm9qVR-PbtNBL31NDOeb8b2h9BLgt8RTMT7K4wxaSUWP95qfToVTLT8CVoQJVkraMefosU_5Bg9r_XXVFHZ0WfomOCOC8X1Av1cXdti3Qgl_rFjzKnJofkdE4x529s6Nh-_nDVDTNHF4nqoc9um5jL5OIVYc29HmA_XEOv1YFO0jc8p307ZCToKtq_w4hCX6Pv5p2-ri3b99fPl6mzdOq7k2EomPZeBWd4RRYPadN4TpjbBKgEkSNs5TUJwnmPGNFCKVdgEKQRwCSAUW6I3-73bkm92UEczxOqg722CvKtGaEo14Y-DjFIpuBAT2O1BV3KtBYLZljjYcmcINrN-c6_fzG6N1uZev-HT3KvDBbvNAP7B1N73BLw-ALY624dik4v1P0c0nX-5RB_2GEzabiMUU12E5MDHAm40PsdHXvIXZceh9A</recordid><startdate>19991015</startdate><enddate>19991015</enddate><creator>Singh, Neeloo</creator><creator>Curran, Martin D</creator><creator>Middleton, Derek</creator><creator>Rastogi, Anil K</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>19991015</creationdate><title>Characterization of kinetoplast DNA minicircles of an Indian isolate of Leishmania donovani</title><author>Singh, Neeloo ; Curran, Martin D ; Middleton, Derek ; Rastogi, Anil K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-737d47f3a45182f8b5dd138bfa86e1f7a5c91ffcd40339e2208fbf766e47ee683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>DNA sequencing</topic><topic>DNA, Kinetoplast - genetics</topic><topic>Evolution</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Indian</topic><topic>Infectious diseases</topic><topic>Inverse PCR</topic><topic>Kinetoplast DNA</topic><topic>Leishmania donovani</topic><topic>Leishmania donovani - genetics</topic><topic>Leishmania donovani - isolation & purification</topic><topic>Leishmaniasis, Visceral - parasitology</topic><topic>Leshmaniasis</topic><topic>Medical sciences</topic><topic>Minicircles</topic><topic>Molecular Sequence Data</topic><topic>Parasitic diseases</topic><topic>Pathogenic</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Protozoal diseases</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Neeloo</creatorcontrib><creatorcontrib>Curran, Martin D</creatorcontrib><creatorcontrib>Middleton, Derek</creatorcontrib><creatorcontrib>Rastogi, Anil K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Neeloo</au><au>Curran, Martin D</au><au>Middleton, Derek</au><au>Rastogi, Anil K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of kinetoplast DNA minicircles of an Indian isolate of Leishmania donovani</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>1999-10-15</date><risdate>1999</risdate><volume>73</volume><issue>3</issue><spage>313</spage><epage>319</epage><pages>313-319</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><coden>ACTRAQ</coden><abstract>Characterization of the kinetoplast DNA minicircles in a human pathogenic Indian isolate of
Leishmania donovani has not been reported previously. Using inverse PCR, we constructed a library of PCR-amplified minicircle variable region from the kinetoplast DNA of this isolate. A combination of restriction enzyme digestion and nucleotide sequence analysis revealed five minicircle DNA sequence classes within the library, one of which was predominant, representing 75% of the kDNA network. Another distinct sequence class represented 15% of the minicircle network. Other minor sequence classes collectively constituted the remaining 10% of the network. Apart from generating basic information on the organisation and distribution of the different sequence classes within the minicircles, the DNA sequence analysis also revealed unique attributes to our minicircles. One was the surprising homology of our isolate (an Old World sp.) with distantly related New World
Leishmania species. Secondly, open reading frames were also identified, indicating the possibility that these minicircles may have more than a structural role to play within the kinetoplast network.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10546849</pmid><doi>10.1016/S0001-706X(99)00036-4</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cloning Cloning, Molecular DNA sequencing DNA, Kinetoplast - genetics Evolution Human protozoal diseases Humans Indian Infectious diseases Inverse PCR Kinetoplast DNA Leishmania donovani Leishmania donovani - genetics Leishmania donovani - isolation & purification Leishmaniasis, Visceral - parasitology Leshmaniasis Medical sciences Minicircles Molecular Sequence Data Parasitic diseases Pathogenic Phylogeny Polymerase Chain Reaction - methods Protozoal diseases Sequence Alignment Sequence Analysis, DNA Tropical medicine |
| title | Characterization of kinetoplast DNA minicircles of an Indian isolate of Leishmania donovani |
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