Probiotics shown to change bacterial community structure in the avian gastrointestinal tract
Culturing and molecular techniques were used to monitor changes in the bacterial flora of the avian gastrointestinal (GI) tract following introduction of genetically modified (GM) and unmodified probiotics. Community hybridization of amplified 16S ribosomal DNA demonstrated that the bacterial flora...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1999-11, Vol.65 (11), p.5134-5138 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Netherwood, T Gilbert, H.J Parker, D.S O'Donnell, A.G |
| description | Culturing and molecular techniques were used to monitor changes in the bacterial flora of the avian gastrointestinal (GI) tract following introduction of genetically modified (GM) and unmodified probiotics. Community hybridization of amplified 16S ribosomal DNA demonstrated that the bacterial flora of the GI tract changed significantly in response to the probiotic treatments. The changes were not detected by culturing. Although both GM and non-GM strains of Enterococcus faecium NCIMB 11508 changed the bacterial flora of the chicken GI tract, they did so differently. Probing the community DNA with an Enterococcus faecalis-specific probe showed that the relative amount of E. faecalis in the total eubacterial population increased in the presence of the non-GM strain and decreased in the presence of the GM probiotic compared with the results obtained with an untreated control group. |
| doi_str_mv | 10.1128/aem.65.11.5134-5138.1999 |
| format | Article |
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Community hybridization of amplified 16S ribosomal DNA demonstrated that the bacterial flora of the GI tract changed significantly in response to the probiotic treatments. The changes were not detected by culturing. Although both GM and non-GM strains of Enterococcus faecium NCIMB 11508 changed the bacterial flora of the chicken GI tract, they did so differently. Probing the community DNA with an Enterococcus faecalis-specific probe showed that the relative amount of E. faecalis in the total eubacterial population increased in the presence of the non-GM strain and decreased in the presence of the GM probiotic compared with the results obtained with an untreated control group.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.65.11.5134-5138.1999</identifier><identifier>PMID: 10543832</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Aging ; animal physiology ; Animal, plant and microbial ecology ; Animals ; Bacillaceae - genetics ; Base Sequence ; Biological and medical sciences ; Cellulase - genetics ; Chickens ; Digestive System - drug effects ; Digestive System - microbiology ; DNA Primers ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; DNA, Ribosomal - genetics ; Drug Resistance, Microbial - genetics ; Enterococcus faecalis - genetics ; Enterococcus faecalis - isolation & purification ; Enterococcus faecalis - physiology ; Enterococcus faecium ; Enterococcus faecium - genetics ; Enterococcus faecium - isolation & purification ; Enterococcus faecium - physiology ; Erythromycin ; Fundamental and applied biological sciences. Psychology ; General Microbial Ecology ; Microbial ecology ; Molecular Sequence Data ; Normal microflora of man and animals. Rumen ; Polymerase Chain Reaction ; Probiotics - pharmacology ; RNA, Ribosomal, 16S - genetics ; rRNA 16S</subject><ispartof>Applied and Environmental Microbiology, 1999-11, Vol.65 (11), p.5134-5138</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright © 1999, American Society for Microbiology 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-d1b09437203af833fac52b96a511863a3af189437fca228b9ff13cf0c4aaa90b3</citedby><cites>FETCH-LOGICAL-c525t-d1b09437203af833fac52b96a511863a3af189437fca228b9ff13cf0c4aaa90b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC91690/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC91690/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3174,3175,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1185212$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10543832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Netherwood, T</creatorcontrib><creatorcontrib>Gilbert, H.J</creatorcontrib><creatorcontrib>Parker, D.S</creatorcontrib><creatorcontrib>O'Donnell, A.G</creatorcontrib><title>Probiotics shown to change bacterial community structure in the avian gastrointestinal tract</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Culturing and molecular techniques were used to monitor changes in the bacterial flora of the avian gastrointestinal (GI) tract following introduction of genetically modified (GM) and unmodified probiotics. Community hybridization of amplified 16S ribosomal DNA demonstrated that the bacterial flora of the GI tract changed significantly in response to the probiotic treatments. The changes were not detected by culturing. Although both GM and non-GM strains of Enterococcus faecium NCIMB 11508 changed the bacterial flora of the chicken GI tract, they did so differently. Probing the community DNA with an Enterococcus faecalis-specific probe showed that the relative amount of E. faecalis in the total eubacterial population increased in the presence of the non-GM strain and decreased in the presence of the GM probiotic compared with the results obtained with an untreated control group.</description><subject>Aging</subject><subject>animal physiology</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Bacillaceae - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cellulase - genetics</subject><subject>Chickens</subject><subject>Digestive System - drug effects</subject><subject>Digestive System - microbiology</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>DNA, Ribosomal - genetics</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Enterococcus faecalis - genetics</subject><subject>Enterococcus faecalis - isolation & purification</subject><subject>Enterococcus faecalis - physiology</subject><subject>Enterococcus faecium</subject><subject>Enterococcus faecium - genetics</subject><subject>Enterococcus faecium - isolation & purification</subject><subject>Enterococcus faecium - physiology</subject><subject>Erythromycin</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General Microbial Ecology</subject><subject>Microbial ecology</subject><subject>Molecular Sequence Data</subject><subject>Normal microflora of man and animals. Rumen</subject><subject>Polymerase Chain Reaction</subject><subject>Probiotics - pharmacology</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkl1rFTEQhoMo9lj9CxqkeLfHfGz2JOBNKfUDKgraOyHMpsnZyG5Sk2xL_71Z9qDHKyFMhpnnnckwQQhTsqWUybdgp20nqr8VlLdNNXJLlVKP0IYSJRvBefcYbQhRqmGsJSfoWc4_CSEt6eRTdEKJaLnkbIN-fE2x97F4k3Ee4n3AJWIzQNhb3IMpNnkYsYnTNAdfHnAuaTZlThb7ig4Ww52HgPdQE9GHYnPxoSpKquLn6ImDMdsXh_sUXb-__H7xsbn68uHTxflVYwQTpbmhPVEt3zHCwUnOHdR4rzoQlMqOQ41SuQDOAGOyV85RbhwxLQAo0vNT9G6tezv3k70xNtT2o75NfoL0oCN4_W8m-EHv451WtFOkyt8c5Cn-musEevLZ2HGEYOOcdacY23HF_gvSXbuctoJyBU2KOSfr_ryFEr1sUJ9fftadqL5eNrgYqZcNVunL41mOhOvKKnB2ACAbGF2CYHz-y1EpGF2w1ys2-P1w75PVkCddP85R2wq9WiEHUcM-1TrX3xihnDAldkvH30XKvAE</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>Netherwood, T</creator><creator>Gilbert, H.J</creator><creator>Parker, D.S</creator><creator>O'Donnell, A.G</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SN</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19991101</creationdate><title>Probiotics shown to change bacterial community structure in the avian gastrointestinal tract</title><author>Netherwood, T ; Gilbert, H.J ; Parker, D.S ; O'Donnell, A.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-d1b09437203af833fac52b96a511863a3af189437fca228b9ff13cf0c4aaa90b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Aging</topic><topic>animal physiology</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Bacillaceae - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cellulase - genetics</topic><topic>Chickens</topic><topic>Digestive System - drug effects</topic><topic>Digestive System - microbiology</topic><topic>DNA Primers</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>DNA, Ribosomal - genetics</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Enterococcus faecalis - genetics</topic><topic>Enterococcus faecalis - isolation & purification</topic><topic>Enterococcus faecalis - physiology</topic><topic>Enterococcus faecium</topic><topic>Enterococcus faecium - genetics</topic><topic>Enterococcus faecium - isolation & purification</topic><topic>Enterococcus faecium - physiology</topic><topic>Erythromycin</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General Microbial Ecology</topic><topic>Microbial ecology</topic><topic>Molecular Sequence Data</topic><topic>Normal microflora of man and animals. Rumen</topic><topic>Polymerase Chain Reaction</topic><topic>Probiotics - pharmacology</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Netherwood, T</creatorcontrib><creatorcontrib>Gilbert, H.J</creatorcontrib><creatorcontrib>Parker, D.S</creatorcontrib><creatorcontrib>O'Donnell, A.G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Netherwood, T</au><au>Gilbert, H.J</au><au>Parker, D.S</au><au>O'Donnell, A.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Probiotics shown to change bacterial community structure in the avian gastrointestinal tract</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>65</volume><issue>11</issue><spage>5134</spage><epage>5138</epage><pages>5134-5138</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Culturing and molecular techniques were used to monitor changes in the bacterial flora of the avian gastrointestinal (GI) tract following introduction of genetically modified (GM) and unmodified probiotics. Community hybridization of amplified 16S ribosomal DNA demonstrated that the bacterial flora of the GI tract changed significantly in response to the probiotic treatments. The changes were not detected by culturing. Although both GM and non-GM strains of Enterococcus faecium NCIMB 11508 changed the bacterial flora of the chicken GI tract, they did so differently. Probing the community DNA with an Enterococcus faecalis-specific probe showed that the relative amount of E. faecalis in the total eubacterial population increased in the presence of the non-GM strain and decreased in the presence of the GM probiotic compared with the results obtained with an untreated control group.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>10543832</pmid><doi>10.1128/aem.65.11.5134-5138.1999</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Aging animal physiology Animal, plant and microbial ecology Animals Bacillaceae - genetics Base Sequence Biological and medical sciences Cellulase - genetics Chickens Digestive System - drug effects Digestive System - microbiology DNA Primers DNA, Bacterial - genetics DNA, Bacterial - isolation & purification DNA, Ribosomal - genetics Drug Resistance, Microbial - genetics Enterococcus faecalis - genetics Enterococcus faecalis - isolation & purification Enterococcus faecalis - physiology Enterococcus faecium Enterococcus faecium - genetics Enterococcus faecium - isolation & purification Enterococcus faecium - physiology Erythromycin Fundamental and applied biological sciences. Psychology General Microbial Ecology Microbial ecology Molecular Sequence Data Normal microflora of man and animals. Rumen Polymerase Chain Reaction Probiotics - pharmacology RNA, Ribosomal, 16S - genetics rRNA 16S |
| title | Probiotics shown to change bacterial community structure in the avian gastrointestinal tract |
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