Specific down-regulation of annexin II expression in human cells interferes with cell proliferation
The protein-tyrosine kinase substrate annexin II is a growth regulated gene whose expression is increased in several human cancers. While the precise function of this protein is not understood, annexin II is proposed to be involved in multiple physiological activities, including DNA synthesis and ce...
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| Veröffentlicht in: | Molecular and cellular biochemistry 1999-09, Vol.199 (1-2), p.139-147 |
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| creator | Chiang, Y Rizzino, A Sibenaller, Z A Wold, M S Vishwanatha, J K |
| description | The protein-tyrosine kinase substrate annexin II is a growth regulated gene whose expression is increased in several human cancers. While the precise function of this protein is not understood, annexin II is proposed to be involved in multiple physiological activities, including DNA synthesis and cell proliferation. Targeted disruption of the annexin II gene affects calcium signaling, tyrosine phosphorylation and apoptosis, indicating the important physiological role of this protein. We used a transient co-transfection assay to regulate annexin II expression in human HeLa, 293 and 293T cells, and measured the effects of annexin II down regulation on DNA synthesis and proliferation. Transfection of cells with an antisense annexin II vector results in inhibition of cell division and proliferation, with concomitant reduction in annexin II message and protein levels. Cellular DNA synthesis is significantly reduced in antisense transfected cells. Replication extracts made from antisense transfected cells have significantly reduced efficiency to support SV40 in vitro DNA replication, while the extracts made from sense transfected cells are fully capable of replication. Our results indicate an important role of annexin II in cellular DNA synthesis and cell proliferation. |
| doi_str_mv | 10.1023/A:1006942128672 |
| format | Article |
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While the precise function of this protein is not understood, annexin II is proposed to be involved in multiple physiological activities, including DNA synthesis and cell proliferation. Targeted disruption of the annexin II gene affects calcium signaling, tyrosine phosphorylation and apoptosis, indicating the important physiological role of this protein. We used a transient co-transfection assay to regulate annexin II expression in human HeLa, 293 and 293T cells, and measured the effects of annexin II down regulation on DNA synthesis and proliferation. Transfection of cells with an antisense annexin II vector results in inhibition of cell division and proliferation, with concomitant reduction in annexin II message and protein levels. Cellular DNA synthesis is significantly reduced in antisense transfected cells. Replication extracts made from antisense transfected cells have significantly reduced efficiency to support SV40 in vitro DNA replication, while the extracts made from sense transfected cells are fully capable of replication. Our results indicate an important role of annexin II in cellular DNA synthesis and cell proliferation.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1023/A:1006942128672</identifier><identifier>PMID: 10544962</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Annexin A2 - genetics ; Annexin A2 - metabolism ; Apoptosis ; Cell division ; Cell Division - genetics ; Cells, Cultured ; Deoxyribonucleic acid ; DNA ; DNA - biosynthesis ; DNA Replication ; DNA, Antisense - genetics ; Down-Regulation ; Gene Expression Regulation ; HeLa Cells - metabolism ; Humans ; Kinases ; Physiology ; Promoter Regions, Genetic ; Proteins ; RNA, Messenger - metabolism ; Simian virus 40 - genetics</subject><ispartof>Molecular and cellular biochemistry, 1999-09, Vol.199 (1-2), p.139-147</ispartof><rights>Kluwer Academic Publishers 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c326t-3993566014f377b8a83cffa300ece9dcbad938442f8b4f35e8c959bd208b8dba3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10544962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiang, Y</creatorcontrib><creatorcontrib>Rizzino, A</creatorcontrib><creatorcontrib>Sibenaller, Z A</creatorcontrib><creatorcontrib>Wold, M S</creatorcontrib><creatorcontrib>Vishwanatha, J K</creatorcontrib><title>Specific down-regulation of annexin II expression in human cells interferes with cell proliferation</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>The protein-tyrosine kinase substrate annexin II is a growth regulated gene whose expression is increased in several human cancers. 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Replication extracts made from antisense transfected cells have significantly reduced efficiency to support SV40 in vitro DNA replication, while the extracts made from sense transfected cells are fully capable of replication. Our results indicate an important role of annexin II in cellular DNA synthesis and cell proliferation.</description><subject>Annexin A2 - genetics</subject><subject>Annexin A2 - metabolism</subject><subject>Apoptosis</subject><subject>Cell division</subject><subject>Cell Division - genetics</subject><subject>Cells, Cultured</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - biosynthesis</subject><subject>DNA Replication</subject><subject>DNA, Antisense - genetics</subject><subject>Down-Regulation</subject><subject>Gene Expression Regulation</subject><subject>HeLa Cells - metabolism</subject><subject>Humans</subject><subject>Kinases</subject><subject>Physiology</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>RNA, Messenger - metabolism</subject><subject>Simian virus 40 - genetics</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkLtPwzAQxi0EoqUwsyGLgS3gV_xgQxWPSpUYgDlyHJu6SpwQJ2r573FLWZhO930_3X13AFxidIsRoXcP9xghrhjBRHJBjsAU54JmTGF1DKaIIpRJLMQEnMW4RijBGJ-CCUY5Y4qTKTBvnTXeeQOrdhOy3n6OtR58G2DroA7Bbn2AiwW02663Me6MJKzGRgdobF3H1A62dza5cOOH1V6FXd_WPon7UefgxOk62otDnYGPp8f3-Uu2fH1ezB-WmaGEDxlViuacI8wcFaKUWlLjnE43WGNVZUpdKSoZI06WCcmtNCpXZUWQLGVVajoDN79z0_av0cahaHzcxdHBtmMsuCKEcyYTeP0PXLdjH1K2QuScMJRzmqCrAzSWja2KrveN7r-Lv9_RH264cYY</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Chiang, Y</creator><creator>Rizzino, A</creator><creator>Sibenaller, Z A</creator><creator>Wold, M S</creator><creator>Vishwanatha, J K</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>Specific down-regulation of annexin II expression in human cells interferes with cell proliferation</title><author>Chiang, Y ; Rizzino, A ; Sibenaller, Z A ; Wold, M S ; Vishwanatha, J K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-3993566014f377b8a83cffa300ece9dcbad938442f8b4f35e8c959bd208b8dba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Annexin A2 - 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Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiang, Y</au><au>Rizzino, A</au><au>Sibenaller, Z A</au><au>Wold, M S</au><au>Vishwanatha, J K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific down-regulation of annexin II expression in human cells interferes with cell proliferation</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>199</volume><issue>1-2</issue><spage>139</spage><epage>147</epage><pages>139-147</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>The protein-tyrosine kinase substrate annexin II is a growth regulated gene whose expression is increased in several human cancers. While the precise function of this protein is not understood, annexin II is proposed to be involved in multiple physiological activities, including DNA synthesis and cell proliferation. Targeted disruption of the annexin II gene affects calcium signaling, tyrosine phosphorylation and apoptosis, indicating the important physiological role of this protein. We used a transient co-transfection assay to regulate annexin II expression in human HeLa, 293 and 293T cells, and measured the effects of annexin II down regulation on DNA synthesis and proliferation. Transfection of cells with an antisense annexin II vector results in inhibition of cell division and proliferation, with concomitant reduction in annexin II message and protein levels. Cellular DNA synthesis is significantly reduced in antisense transfected cells. Replication extracts made from antisense transfected cells have significantly reduced efficiency to support SV40 in vitro DNA replication, while the extracts made from sense transfected cells are fully capable of replication. Our results indicate an important role of annexin II in cellular DNA synthesis and cell proliferation.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>10544962</pmid><doi>10.1023/A:1006942128672</doi><tpages>9</tpages></addata></record> |
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| ispartof | Molecular and cellular biochemistry, 1999-09, Vol.199 (1-2), p.139-147 |
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| subjects | Annexin A2 - genetics Annexin A2 - metabolism Apoptosis Cell division Cell Division - genetics Cells, Cultured Deoxyribonucleic acid DNA DNA - biosynthesis DNA Replication DNA, Antisense - genetics Down-Regulation Gene Expression Regulation HeLa Cells - metabolism Humans Kinases Physiology Promoter Regions, Genetic Proteins RNA, Messenger - metabolism Simian virus 40 - genetics |
| title | Specific down-regulation of annexin II expression in human cells interferes with cell proliferation |
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