Evaluation of recombinant sporozoite antigen SPAG‐1 as a vaccine candidate against Theileria annulata by the use of different delivery systems
Summary The major sporozoite surface antigen of Theileria annulata (SPAG‐1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG‐1 expressed in different systems and presented in different adjuvants. The antigen...
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| Veröffentlicht in: | Tropical medicine & international health 1999-09, Vol.4 (9), p.A71-A77 |
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| creator | Boulter, Nicola Brown, Duncan Wilkie, Gwen Williamson, Susanna Kirvar, Erol Knight, Pamela Glass, Elizabeth Campbell, John Morzaria, Subhash Nene, Vish Musoke, Anthony D'oliveira, Christine Gubbels, Marc‐Jan Jongejan, Frans Hall, Roger |
| description | Summary The major sporozoite surface antigen of Theileria annulata (SPAG‐1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG‐1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both β‐galactosidase and hepatitis B core antigen fusions or as a full‐length form expressed as a GST fusion with an N terminal His6 tag. We used different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham, which we call SKBA. The data point to the conclusion that SPAG‐1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine. |
| doi_str_mv | 10.1046/j.1365-3156.1999.00453.x |
| format | Article |
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In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG‐1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both β‐galactosidase and hepatitis B core antigen fusions or as a full‐length form expressed as a GST fusion with an N terminal His6 tag. We used different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham, which we call SKBA. The data point to the conclusion that SPAG‐1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine.</description><identifier>ISSN: 1360-2276</identifier><identifier>EISSN: 1365-3156</identifier><identifier>DOI: 10.1046/j.1365-3156.1999.00453.x</identifier><identifier>PMID: 10540314</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject><![CDATA[Animals ; Antigens, Protozoan - administration & dosage ; Antigens, Protozoan - pharmacology ; Cattle ; Cattle Diseases - prevention & control ; Freund's Adjuvant - administration & dosage ; Freund's Adjuvant - pharmacology ; protection ; Protozoan Proteins - administration & dosage ; Protozoan Proteins - pharmacology ; Protozoan Vaccines - administration & dosage ; Protozoan Vaccines - pharmacology ; Recombinant Proteins - administration & dosage ; Recombinant Proteins - pharmacology ; sporozoite antigen (SPAG‐1) ; Theileria annulata ; Theileria annulata - immunology ; Theileriasis - prevention & control ; vaccine]]></subject><ispartof>Tropical medicine & international health, 1999-09, Vol.4 (9), p.A71-A77</ispartof><rights>1999 Blackwell Science Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2613-c8f792019e9de72c2dc158ecc6a46de91a12a39ca66adcb88d0afad73bf0620f3</citedby><cites>FETCH-LOGICAL-c2613-c8f792019e9de72c2dc158ecc6a46de91a12a39ca66adcb88d0afad73bf0620f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-3156.1999.00453.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-3156.1999.00453.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10540314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boulter, Nicola</creatorcontrib><creatorcontrib>Brown, Duncan</creatorcontrib><creatorcontrib>Wilkie, Gwen</creatorcontrib><creatorcontrib>Williamson, Susanna</creatorcontrib><creatorcontrib>Kirvar, Erol</creatorcontrib><creatorcontrib>Knight, Pamela</creatorcontrib><creatorcontrib>Glass, Elizabeth</creatorcontrib><creatorcontrib>Campbell, John</creatorcontrib><creatorcontrib>Morzaria, Subhash</creatorcontrib><creatorcontrib>Nene, Vish</creatorcontrib><creatorcontrib>Musoke, Anthony</creatorcontrib><creatorcontrib>D'oliveira, Christine</creatorcontrib><creatorcontrib>Gubbels, Marc‐Jan</creatorcontrib><creatorcontrib>Jongejan, Frans</creatorcontrib><creatorcontrib>Hall, Roger</creatorcontrib><title>Evaluation of recombinant sporozoite antigen SPAG‐1 as a vaccine candidate against Theileria annulata by the use of different delivery systems</title><title>Tropical medicine & international health</title><addtitle>Trop Med Int Health</addtitle><description>Summary The major sporozoite surface antigen of Theileria annulata (SPAG‐1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG‐1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both β‐galactosidase and hepatitis B core antigen fusions or as a full‐length form expressed as a GST fusion with an N terminal His6 tag. We used different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham, which we call SKBA. The data point to the conclusion that SPAG‐1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine.</description><subject>Animals</subject><subject>Antigens, Protozoan - administration & dosage</subject><subject>Antigens, Protozoan - pharmacology</subject><subject>Cattle</subject><subject>Cattle Diseases - prevention & control</subject><subject>Freund's Adjuvant - administration & dosage</subject><subject>Freund's Adjuvant - pharmacology</subject><subject>protection</subject><subject>Protozoan Proteins - administration & dosage</subject><subject>Protozoan Proteins - pharmacology</subject><subject>Protozoan Vaccines - administration & dosage</subject><subject>Protozoan Vaccines - pharmacology</subject><subject>Recombinant Proteins - administration & dosage</subject><subject>Recombinant Proteins - pharmacology</subject><subject>sporozoite antigen (SPAG‐1)</subject><subject>Theileria annulata</subject><subject>Theileria annulata - immunology</subject><subject>Theileriasis - prevention & control</subject><subject>vaccine</subject><issn>1360-2276</issn><issn>1365-3156</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1O3DAUha2qFVDKK1RedZfgn8QTS90gRCkSVSsxXVs39jV4lJ-pnQykqz4Cz8iTNGFQ1SUrX-t-5x7pHEIoZzlnhTrd5FyqMpO8VDnXWueMFaXMH96Qo3-Lt88zy4RYqUPyPqUNm6miVAfkkLOyYJIXR-TxYgfNCEPoO9p7GtH2bR066Aaatn3sf_dhQDp_wy129ObH2eXTn0dOIVGgO7A2dEgtdC44WLhbCF0a6PoOQ4MxwKzsxgYGoPVEhzukY8LFxwXvMeLs4rAJO4wTTVMasE0fyDsPTcKTl_eY_PxysT7_ml1_v7w6P7vOrFBcZrbyKy0Y16gdroQVzvKyQmsVFMqh5sAFSG1BKXC2rirHwINbydozJZiXx-TT_u429r9GTINpQ7LYNNBhPyajtBClLPQMVnvQxj6liN5sY2ghToYzs7RhNmYJ3Syhm6UN89yGeZilH188xrpF959wH_8MfN4D93Nc06sPm_W3q3mQfwFqGJzm</recordid><startdate>199909</startdate><enddate>199909</enddate><creator>Boulter, Nicola</creator><creator>Brown, Duncan</creator><creator>Wilkie, Gwen</creator><creator>Williamson, Susanna</creator><creator>Kirvar, Erol</creator><creator>Knight, Pamela</creator><creator>Glass, Elizabeth</creator><creator>Campbell, John</creator><creator>Morzaria, Subhash</creator><creator>Nene, Vish</creator><creator>Musoke, Anthony</creator><creator>D'oliveira, Christine</creator><creator>Gubbels, Marc‐Jan</creator><creator>Jongejan, Frans</creator><creator>Hall, Roger</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199909</creationdate><title>Evaluation of recombinant sporozoite antigen SPAG‐1 as a vaccine candidate against Theileria annulata by the use of different delivery systems</title><author>Boulter, Nicola ; Brown, Duncan ; Wilkie, Gwen ; Williamson, Susanna ; Kirvar, Erol ; Knight, Pamela ; Glass, Elizabeth ; Campbell, John ; Morzaria, Subhash ; Nene, Vish ; Musoke, Anthony ; D'oliveira, Christine ; Gubbels, Marc‐Jan ; Jongejan, Frans ; Hall, Roger</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2613-c8f792019e9de72c2dc158ecc6a46de91a12a39ca66adcb88d0afad73bf0620f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antigens, Protozoan - administration & dosage</topic><topic>Antigens, Protozoan - pharmacology</topic><topic>Cattle</topic><topic>Cattle Diseases - prevention & control</topic><topic>Freund's Adjuvant - administration & dosage</topic><topic>Freund's Adjuvant - pharmacology</topic><topic>protection</topic><topic>Protozoan Proteins - administration & dosage</topic><topic>Protozoan Proteins - pharmacology</topic><topic>Protozoan Vaccines - administration & dosage</topic><topic>Protozoan Vaccines - pharmacology</topic><topic>Recombinant Proteins - administration & dosage</topic><topic>Recombinant Proteins - pharmacology</topic><topic>sporozoite antigen (SPAG‐1)</topic><topic>Theileria annulata</topic><topic>Theileria annulata - immunology</topic><topic>Theileriasis - prevention & control</topic><topic>vaccine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boulter, Nicola</creatorcontrib><creatorcontrib>Brown, Duncan</creatorcontrib><creatorcontrib>Wilkie, Gwen</creatorcontrib><creatorcontrib>Williamson, Susanna</creatorcontrib><creatorcontrib>Kirvar, Erol</creatorcontrib><creatorcontrib>Knight, Pamela</creatorcontrib><creatorcontrib>Glass, Elizabeth</creatorcontrib><creatorcontrib>Campbell, John</creatorcontrib><creatorcontrib>Morzaria, Subhash</creatorcontrib><creatorcontrib>Nene, Vish</creatorcontrib><creatorcontrib>Musoke, Anthony</creatorcontrib><creatorcontrib>D'oliveira, Christine</creatorcontrib><creatorcontrib>Gubbels, Marc‐Jan</creatorcontrib><creatorcontrib>Jongejan, Frans</creatorcontrib><creatorcontrib>Hall, Roger</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Tropical medicine & international health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boulter, Nicola</au><au>Brown, Duncan</au><au>Wilkie, Gwen</au><au>Williamson, Susanna</au><au>Kirvar, Erol</au><au>Knight, Pamela</au><au>Glass, Elizabeth</au><au>Campbell, John</au><au>Morzaria, Subhash</au><au>Nene, Vish</au><au>Musoke, Anthony</au><au>D'oliveira, Christine</au><au>Gubbels, Marc‐Jan</au><au>Jongejan, Frans</au><au>Hall, Roger</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of recombinant sporozoite antigen SPAG‐1 as a vaccine candidate against Theileria annulata by the use of different delivery systems</atitle><jtitle>Tropical medicine & international health</jtitle><addtitle>Trop Med Int Health</addtitle><date>1999-09</date><risdate>1999</risdate><volume>4</volume><issue>9</issue><spage>A71</spage><epage>A77</epage><pages>A71-A77</pages><issn>1360-2276</issn><eissn>1365-3156</eissn><abstract>Summary The major sporozoite surface antigen of Theileria annulata (SPAG‐1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG‐1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both β‐galactosidase and hepatitis B core antigen fusions or as a full‐length form expressed as a GST fusion with an N terminal His6 tag. We used different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham, which we call SKBA. The data point to the conclusion that SPAG‐1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>10540314</pmid><doi>10.1046/j.1365-3156.1999.00453.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1360-2276 |
| ispartof | Tropical medicine & international health, 1999-09, Vol.4 (9), p.A71-A77 |
| issn | 1360-2276 1365-3156 |
| language | eng |
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| source | MEDLINE; Wiley-Blackwell Open Access Backfiles (Open Access); Free E-Journal (出版社公開部分のみ）; Wiley Online Library All Journals |
| subjects | Animals Antigens, Protozoan - administration & dosage Antigens, Protozoan - pharmacology Cattle Cattle Diseases - prevention & control Freund's Adjuvant - administration & dosage Freund's Adjuvant - pharmacology protection Protozoan Proteins - administration & dosage Protozoan Proteins - pharmacology Protozoan Vaccines - administration & dosage Protozoan Vaccines - pharmacology Recombinant Proteins - administration & dosage Recombinant Proteins - pharmacology sporozoite antigen (SPAG‐1) Theileria annulata Theileria annulata - immunology Theileriasis - prevention & control vaccine |
| title | Evaluation of recombinant sporozoite antigen SPAG‐1 as a vaccine candidate against Theileria annulata by the use of different delivery systems |
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