Failure of secondary infection with American genotype dengue 2 to cause dengue haemorrhagic fever
Population-based epidemiological studies have shown that infection with dengue type 2 (DEN-2) virus in individuals previously infected with a different serotype of the virus is a major risk factor for dengue haemorrhagic fever and dengue shock syndrome. However, the western hemisphere was spared epi...
Gespeichert in:
| Veröffentlicht in: | The Lancet (British edition) 1999-10, Vol.354 (9188), p.1431-1434 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1434 |
|---|---|
| container_issue | 9188 |
| container_start_page | 1431 |
| container_title | The Lancet (British edition) |
| container_volume | 354 |
| creator | Watts, Douglas M Porter, Kevin R Putvatana, Pavithat Vasquez, Bruno Calampa, Carlos Hayes, Curtis G Halstead, Scott B |
| description | Population-based epidemiological studies have shown that infection with dengue type 2 (DEN-2) virus in individuals previously infected with a different serotype of the virus is a major risk factor for dengue haemorrhagic fever and dengue shock syndrome. However, the western hemisphere was spared epidemics of these two syndromes, until the introduction of a southeast Asian DEN-2 genotype. Possibly American DEN-2 genotype strains lacked properties necessary to cause severe disease. We report on a major epidemic of DEN-2 in Peru in 1995, about 5 years after an epidemic of DEN-1 in the same population.
In Iquitos, a city of 344 686 inhabitants in Peru, cases of dengue fever were studied prospectively from 1990. Acute phase of illness serum samples from patients were tested for virus in C6/36 cells, and virus isolates were identified by immunofluorescence. Isolates of dengue 2 virus obtained from patients during an outbreak of mild febrile illness in 1995 were sequenced to determine the genotype. Serological analysis of paired samples from the patients was done with an]gM capture ELISA and an indirect]gG ELISA In addition, serum samples collected annually between 1993 and 1996 from a large cohort of students were tested for dengue]gG antibody by an ELISA Serum samples from a random sample of 129 students from this cohort were tested for dengue neutralising antibodies to quantify the serotype specific infection rates.
Among the 129 students (aged 7-20 years in 1993) who had serum samples available before and after the epidemic, 78 (60·5%) had a secondary DEN-2 virus infection. By extrapolation, 49 266 of the 81 479 children (aged 5-14 years) in Iquitos would have experienced such infections. From previous studies, between 887 and 10 247 cases of dengue haemorrhagic fever and dengue shock syndrome would have been expected. No cases were found. DEN-2 isolates were of the American genotype.
This prospective study shows that secondary infection by the American DEN-2 genotype did not cause dengue haemorrhagic fever and dengue shock syndrome |
| doi_str_mv | 10.1016/S0140-6736(99)04015-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69221824</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0140673699040155</els_id><sourcerecordid>45813970</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-84165299850055d98ba94483831c0fa42983fc46c41fc8cfb316846d4cf789bd3</originalsourceid><addsrcrecordid>eNqF0V1rFDEUBuAgil2rP0EJIqIXo8nMyddVKcWqUPBCBe9CNnOymzKTrMlMpf_e2e5SxRuvAuE5h8P7EvKcs3eccfn-K-PAGqk6-caYtwwYF414QFYcFDQC1I-HZHVPTsiTWq8ZYyCZeExOOBPQScVWxF26OMwFaQ60os-pd-WWxhTQTzEn-itOW3o-YoneJbrBlKfbHdIe02ZG2tIpU-_mev-zdTjmUrZuEz0NeIPlKXkU3FDx2fE9Jd8vP3y7-NRcffn4-eL8qvHA1dRo4FK0xmjBmBC90WtnAHSnO-5ZcNAa3QUPctHBax_WHZcaZA8-KG3WfXdKXh_27kr-OWOd7Birx2FwCfNcrTRty3ULC3z5D7zOc0nLbZYbw0AovkfigHzJtRYMdlfiuGRjObP7AuxdAXafrjXG3hVgxTL34rh8Xo_Y_zV1SHwBr47AVe-GUFzysf5xRkkFe3Z2YLhEdhOx2OojJo99LEs1ts_xP5f8BgwRoOk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>199045714</pqid></control><display><type>article</type><title>Failure of secondary infection with American genotype dengue 2 to cause dengue haemorrhagic fever</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Business Source Complete</source><source>ProQuest Central UK/Ireland</source><creator>Watts, Douglas M ; Porter, Kevin R ; Putvatana, Pavithat ; Vasquez, Bruno ; Calampa, Carlos ; Hayes, Curtis G ; Halstead, Scott B</creator><creatorcontrib>Watts, Douglas M ; Porter, Kevin R ; Putvatana, Pavithat ; Vasquez, Bruno ; Calampa, Carlos ; Hayes, Curtis G ; Halstead, Scott B</creatorcontrib><description>Population-based epidemiological studies have shown that infection with dengue type 2 (DEN-2) virus in individuals previously infected with a different serotype of the virus is a major risk factor for dengue haemorrhagic fever and dengue shock syndrome. However, the western hemisphere was spared epidemics of these two syndromes, until the introduction of a southeast Asian DEN-2 genotype. Possibly American DEN-2 genotype strains lacked properties necessary to cause severe disease. We report on a major epidemic of DEN-2 in Peru in 1995, about 5 years after an epidemic of DEN-1 in the same population.
In Iquitos, a city of 344 686 inhabitants in Peru, cases of dengue fever were studied prospectively from 1990. Acute phase of illness serum samples from patients were tested for virus in C6/36 cells, and virus isolates were identified by immunofluorescence. Isolates of dengue 2 virus obtained from patients during an outbreak of mild febrile illness in 1995 were sequenced to determine the genotype. Serological analysis of paired samples from the patients was done with an]gM capture ELISA and an indirect]gG ELISA In addition, serum samples collected annually between 1993 and 1996 from a large cohort of students were tested for dengue]gG antibody by an ELISA Serum samples from a random sample of 129 students from this cohort were tested for dengue neutralising antibodies to quantify the serotype specific infection rates.
Among the 129 students (aged 7-20 years in 1993) who had serum samples available before and after the epidemic, 78 (60·5%) had a secondary DEN-2 virus infection. By extrapolation, 49 266 of the 81 479 children (aged 5-14 years) in Iquitos would have experienced such infections. From previous studies, between 887 and 10 247 cases of dengue haemorrhagic fever and dengue shock syndrome would have been expected. No cases were found. DEN-2 isolates were of the American genotype.
This prospective study shows that secondary infection by the American DEN-2 genotype did not cause dengue haemorrhagic fever and dengue shock syndrome</description><identifier>ISSN: 0140-6736</identifier><identifier>EISSN: 1474-547X</identifier><identifier>DOI: 10.1016/S0140-6736(99)04015-5</identifier><identifier>PMID: 10543670</identifier><identifier>CODEN: LANCAO</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Arboviroses ; Biological and medical sciences ; Child ; Dengue - epidemiology ; Dengue - virology ; Dengue fevers ; Dengue Virus - classification ; Dengue Virus - pathogenicity ; Disease Outbreaks ; Epidemics ; Epidemiology ; Female ; Fever ; Genetics ; Genotype ; Hemorrhage ; Human viral diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Peru - epidemiology ; Risk factors ; Severe Dengue - epidemiology ; Severe Dengue - virology ; Superinfection ; Tropical medicine ; Tropical viral diseases ; Vector-borne diseases ; Viral diseases</subject><ispartof>The Lancet (British edition), 1999-10, Vol.354 (9188), p.1431-1434</ispartof><rights>1999 Elsevier Ltd</rights><rights>1999 INIST-CNRS</rights><rights>Copyright Lancet Ltd. Oct 23, 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-84165299850055d98ba94483831c0fa42983fc46c41fc8cfb316846d4cf789bd3</citedby><cites>FETCH-LOGICAL-c417t-84165299850055d98ba94483831c0fa42983fc46c41fc8cfb316846d4cf789bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/199045714?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976,64364,64366,64368,72218</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1976740$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10543670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watts, Douglas M</creatorcontrib><creatorcontrib>Porter, Kevin R</creatorcontrib><creatorcontrib>Putvatana, Pavithat</creatorcontrib><creatorcontrib>Vasquez, Bruno</creatorcontrib><creatorcontrib>Calampa, Carlos</creatorcontrib><creatorcontrib>Hayes, Curtis G</creatorcontrib><creatorcontrib>Halstead, Scott B</creatorcontrib><title>Failure of secondary infection with American genotype dengue 2 to cause dengue haemorrhagic fever</title><title>The Lancet (British edition)</title><addtitle>Lancet</addtitle><description>Population-based epidemiological studies have shown that infection with dengue type 2 (DEN-2) virus in individuals previously infected with a different serotype of the virus is a major risk factor for dengue haemorrhagic fever and dengue shock syndrome. However, the western hemisphere was spared epidemics of these two syndromes, until the introduction of a southeast Asian DEN-2 genotype. Possibly American DEN-2 genotype strains lacked properties necessary to cause severe disease. We report on a major epidemic of DEN-2 in Peru in 1995, about 5 years after an epidemic of DEN-1 in the same population.
In Iquitos, a city of 344 686 inhabitants in Peru, cases of dengue fever were studied prospectively from 1990. Acute phase of illness serum samples from patients were tested for virus in C6/36 cells, and virus isolates were identified by immunofluorescence. Isolates of dengue 2 virus obtained from patients during an outbreak of mild febrile illness in 1995 were sequenced to determine the genotype. Serological analysis of paired samples from the patients was done with an]gM capture ELISA and an indirect]gG ELISA In addition, serum samples collected annually between 1993 and 1996 from a large cohort of students were tested for dengue]gG antibody by an ELISA Serum samples from a random sample of 129 students from this cohort were tested for dengue neutralising antibodies to quantify the serotype specific infection rates.
Among the 129 students (aged 7-20 years in 1993) who had serum samples available before and after the epidemic, 78 (60·5%) had a secondary DEN-2 virus infection. By extrapolation, 49 266 of the 81 479 children (aged 5-14 years) in Iquitos would have experienced such infections. From previous studies, between 887 and 10 247 cases of dengue haemorrhagic fever and dengue shock syndrome would have been expected. No cases were found. DEN-2 isolates were of the American genotype.
This prospective study shows that secondary infection by the American DEN-2 genotype did not cause dengue haemorrhagic fever and dengue shock syndrome</description><subject>Adolescent</subject><subject>Adult</subject><subject>Arboviroses</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Dengue - epidemiology</subject><subject>Dengue - virology</subject><subject>Dengue fevers</subject><subject>Dengue Virus - classification</subject><subject>Dengue Virus - pathogenicity</subject><subject>Disease Outbreaks</subject><subject>Epidemics</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Fever</subject><subject>Genetics</subject><subject>Genotype</subject><subject>Hemorrhage</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Peru - epidemiology</subject><subject>Risk factors</subject><subject>Severe Dengue - epidemiology</subject><subject>Severe Dengue - virology</subject><subject>Superinfection</subject><subject>Tropical medicine</subject><subject>Tropical viral diseases</subject><subject>Vector-borne diseases</subject><subject>Viral diseases</subject><issn>0140-6736</issn><issn>1474-547X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0V1rFDEUBuAgil2rP0EJIqIXo8nMyddVKcWqUPBCBe9CNnOymzKTrMlMpf_e2e5SxRuvAuE5h8P7EvKcs3eccfn-K-PAGqk6-caYtwwYF414QFYcFDQC1I-HZHVPTsiTWq8ZYyCZeExOOBPQScVWxF26OMwFaQ60os-pd-WWxhTQTzEn-itOW3o-YoneJbrBlKfbHdIe02ZG2tIpU-_mev-zdTjmUrZuEz0NeIPlKXkU3FDx2fE9Jd8vP3y7-NRcffn4-eL8qvHA1dRo4FK0xmjBmBC90WtnAHSnO-5ZcNAa3QUPctHBax_WHZcaZA8-KG3WfXdKXh_27kr-OWOd7Birx2FwCfNcrTRty3ULC3z5D7zOc0nLbZYbw0AovkfigHzJtRYMdlfiuGRjObP7AuxdAXafrjXG3hVgxTL34rh8Xo_Y_zV1SHwBr47AVe-GUFzysf5xRkkFe3Z2YLhEdhOx2OojJo99LEs1ts_xP5f8BgwRoOk</recordid><startdate>19991023</startdate><enddate>19991023</enddate><creator>Watts, Douglas M</creator><creator>Porter, Kevin R</creator><creator>Putvatana, Pavithat</creator><creator>Vasquez, Bruno</creator><creator>Calampa, Carlos</creator><creator>Hayes, Curtis G</creator><creator>Halstead, Scott B</creator><general>Elsevier Ltd</general><general>Lancet</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0TT</scope><scope>0TZ</scope><scope>0U~</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88C</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8C2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AN0</scope><scope>ASE</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FPQ</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K6X</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>KB~</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>19991023</creationdate><title>Failure of secondary infection with American genotype dengue 2 to cause dengue haemorrhagic fever</title><author>Watts, Douglas M ; Porter, Kevin R ; Putvatana, Pavithat ; Vasquez, Bruno ; Calampa, Carlos ; Hayes, Curtis G ; Halstead, Scott B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-84165299850055d98ba94483831c0fa42983fc46c41fc8cfb316846d4cf789bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Arboviroses</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Dengue - epidemiology</topic><topic>Dengue - virology</topic><topic>Dengue fevers</topic><topic>Dengue Virus - classification</topic><topic>Dengue Virus - pathogenicity</topic><topic>Disease Outbreaks</topic><topic>Epidemics</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Fever</topic><topic>Genetics</topic><topic>Genotype</topic><topic>Hemorrhage</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Peru - epidemiology</topic><topic>Risk factors</topic><topic>Severe Dengue - epidemiology</topic><topic>Severe Dengue - virology</topic><topic>Superinfection</topic><topic>Tropical medicine</topic><topic>Tropical viral diseases</topic><topic>Vector-borne diseases</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watts, Douglas M</creatorcontrib><creatorcontrib>Porter, Kevin R</creatorcontrib><creatorcontrib>Putvatana, Pavithat</creatorcontrib><creatorcontrib>Vasquez, Bruno</creatorcontrib><creatorcontrib>Calampa, Carlos</creatorcontrib><creatorcontrib>Hayes, Curtis G</creatorcontrib><creatorcontrib>Halstead, Scott B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>News PRO</collection><collection>Pharma and Biotech Premium PRO</collection><collection>Global News & ABI/Inform Professional</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Lancet Titles</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>British Nursing Index</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>British Nursing Index (BNI) (1985 to Present)</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>British Nursing Index</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Newsstand Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>The Lancet (British edition)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watts, Douglas M</au><au>Porter, Kevin R</au><au>Putvatana, Pavithat</au><au>Vasquez, Bruno</au><au>Calampa, Carlos</au><au>Hayes, Curtis G</au><au>Halstead, Scott B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Failure of secondary infection with American genotype dengue 2 to cause dengue haemorrhagic fever</atitle><jtitle>The Lancet (British edition)</jtitle><addtitle>Lancet</addtitle><date>1999-10-23</date><risdate>1999</risdate><volume>354</volume><issue>9188</issue><spage>1431</spage><epage>1434</epage><pages>1431-1434</pages><issn>0140-6736</issn><eissn>1474-547X</eissn><coden>LANCAO</coden><abstract>Population-based epidemiological studies have shown that infection with dengue type 2 (DEN-2) virus in individuals previously infected with a different serotype of the virus is a major risk factor for dengue haemorrhagic fever and dengue shock syndrome. However, the western hemisphere was spared epidemics of these two syndromes, until the introduction of a southeast Asian DEN-2 genotype. Possibly American DEN-2 genotype strains lacked properties necessary to cause severe disease. We report on a major epidemic of DEN-2 in Peru in 1995, about 5 years after an epidemic of DEN-1 in the same population.
In Iquitos, a city of 344 686 inhabitants in Peru, cases of dengue fever were studied prospectively from 1990. Acute phase of illness serum samples from patients were tested for virus in C6/36 cells, and virus isolates were identified by immunofluorescence. Isolates of dengue 2 virus obtained from patients during an outbreak of mild febrile illness in 1995 were sequenced to determine the genotype. Serological analysis of paired samples from the patients was done with an]gM capture ELISA and an indirect]gG ELISA In addition, serum samples collected annually between 1993 and 1996 from a large cohort of students were tested for dengue]gG antibody by an ELISA Serum samples from a random sample of 129 students from this cohort were tested for dengue neutralising antibodies to quantify the serotype specific infection rates.
Among the 129 students (aged 7-20 years in 1993) who had serum samples available before and after the epidemic, 78 (60·5%) had a secondary DEN-2 virus infection. By extrapolation, 49 266 of the 81 479 children (aged 5-14 years) in Iquitos would have experienced such infections. From previous studies, between 887 and 10 247 cases of dengue haemorrhagic fever and dengue shock syndrome would have been expected. No cases were found. DEN-2 isolates were of the American genotype.
This prospective study shows that secondary infection by the American DEN-2 genotype did not cause dengue haemorrhagic fever and dengue shock syndrome</abstract><cop>London</cop><pub>Elsevier Ltd</pub><pmid>10543670</pmid><doi>10.1016/S0140-6736(99)04015-5</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0140-6736 |
| ispartof | The Lancet (British edition), 1999-10, Vol.354 (9188), p.1431-1434 |
| issn | 0140-6736 1474-547X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69221824 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Business Source Complete; ProQuest Central UK/Ireland |
| subjects | Adolescent Adult Arboviroses Biological and medical sciences Child Dengue - epidemiology Dengue - virology Dengue fevers Dengue Virus - classification Dengue Virus - pathogenicity Disease Outbreaks Epidemics Epidemiology Female Fever Genetics Genotype Hemorrhage Human viral diseases Humans Infectious diseases Male Medical sciences Peru - epidemiology Risk factors Severe Dengue - epidemiology Severe Dengue - virology Superinfection Tropical medicine Tropical viral diseases Vector-borne diseases Viral diseases |
| title | Failure of secondary infection with American genotype dengue 2 to cause dengue haemorrhagic fever |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T09%3A11%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Failure%20of%20secondary%20infection%20with%20American%20genotype%20dengue%202%20to%20cause%20dengue%20haemorrhagic%20fever&rft.jtitle=The%20Lancet%20(British%20edition)&rft.au=Watts,%20Douglas%20M&rft.date=1999-10-23&rft.volume=354&rft.issue=9188&rft.spage=1431&rft.epage=1434&rft.pages=1431-1434&rft.issn=0140-6736&rft.eissn=1474-547X&rft.coden=LANCAO&rft_id=info:doi/10.1016/S0140-6736(99)04015-5&rft_dat=%3Cproquest_cross%3E45813970%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=199045714&rft_id=info:pmid/10543670&rft_els_id=S0140673699040155&rfr_iscdi=true |