Selective deuteration of tryptophan and methionine residues in maltose binding protein: a model system for neutron scattering

We describe methods that have been developed within the ILL-EMBL Deuteration Laboratory for the production of maltose binding protein (MBP) that has been selectively labelled either with deuterated tryptophan or deuterated methionine (single labelling), or both (double labelling). MBP is used as an...

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Veröffentlicht in:	European biophysics journal 2008-07, Vol.37 (6), p.815-822
Hauptverfasser:	Laux, Valerie, Callow, Phil, Svergun, Dmitri I., Timmins, Peter A., Forsyth, V. Trevor, Haertlein, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biophysics Carrier Proteins - chemistry Cell Biology Deuterium - chemistry E coli Life Sciences Light scattering Maltose-Binding Proteins Mass spectroscopy Membrane Biology Methionine - chemistry Molecular biology Nanotechnology Neurobiology Neutron Diffraction - methods Neutrons Original Paper Protein Binding Proteins Residues Scattering Scattering, Small Angle Staining and Labeling - methods Tryptophan - chemistry
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