Occurrence of Borrelia spp. in culture of embryonic cells of the tick Boophilus microplus (Acari: Ixodidae) in the state of the Mato Grosso do Sul, Brazil

Occurrence of Borrelia spp. in culture of embryonic cells of the tick Boophilus microplus (Acari: Ixodidae) in the state of the Mato Grosso do Sul, Brazil

The aim of the present work was to report the occurrence of Borrelia spp. in embryonic cell cultures from naturally infected Boophilus microplus. Seven days after the beginning of a primary culture of embryonic cells of B. microplus at 31 degrees C was noted that the cells start suffering degenerati...

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Veröffentlicht in:Revista brasileira de parasitologia veterinaria 2008-01, Vol.17 (1), p.50-52
Hauptverfasser: de Rezende, Jania, Kessler, Raul H, Soares, Cleber O, Martins, Odair P
Format: Artikel
Sprache:por
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Animals
Borrelia - isolation & purification
Cells, Cultured - microbiology
Ixodidae - cytology
Ixodidae - embryology
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container_title Revista brasileira de parasitologia veterinaria
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creator de Rezende, Jania
Kessler, Raul H
Soares, Cleber O
Martins, Odair P
description The aim of the present work was to report the occurrence of Borrelia spp. in embryonic cell cultures from naturally infected Boophilus microplus. Seven days after the beginning of a primary culture of embryonic cells of B. microplus at 31 degrees C was noted that the cells start suffering degeneration. Under examination at phase contrast microscope, the presence of prolongated microorganisms with great mobility was detected. Microscopic slides of the culture supernatant, hemolymph and egg mass, were stained by May Grünwald-Giemsa, allowing the visualization of the spirochetes. The morphologic examination of the microorganism and its visualization in. B. microplus, suggest to be Borrelia spp.
doi_str_mv 10.1590/S1984-29612008000100011
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subjects Animals
Borrelia - isolation & purification
Cells, Cultured - microbiology
Ixodidae - cytology
Ixodidae - embryology
title Occurrence of Borrelia spp. in culture of embryonic cells of the tick Boophilus microplus (Acari: Ixodidae) in the state of the Mato Grosso do Sul, Brazil
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