Molecular cloning and tissue-specific expression of two cDNAs encoding polyketide synthases from Hypericum perforatum
Two previously uncharacterized cDNAs encoding for polyketide synthases (PKSs), designated as HpPKS1 and HpPKS2, were isolated from Hypericum perforatum. The full-length HpPKS1 was 1573 bp containing an open reading frame (ORF) of 1161 bp encoding for a 386 amino acid protein. The full-length cDNA of...
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| Veröffentlicht in: | Journal of plant physiology 2008-01, Vol.165 (10), p.1079-1086 |
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| creator | Karppinen, Katja Hohtola, Anja |
| description | Two previously uncharacterized cDNAs encoding for polyketide synthases (PKSs), designated as
HpPKS1 and
HpPKS2, were isolated from
Hypericum perforatum. The full-length
HpPKS1 was 1573
bp containing an open reading frame (ORF) of 1161
bp encoding for a 386 amino acid protein. The full-length cDNA of
HpPKS2 was 1559
bp with an ORF of 1182
bp encoding for a 393 amino acid protein. The highly conserved catalytic amino acid residues common to plant-specific PKSs were preserved in both genes.
HpPKS1 and
HpPKS2 exhibited distinct tissue-specific expression patterns in
H. perforatum. The
HpPKS1 expression was highest in flower buds and lowest in root tissues. The expression of
HpPKS2 was found to be high in flower buds and leaf margins and low in leaf interior parts, stems and roots. The expression of the
HpPKS1 was found to correlate with the concentrations of hyperforin and adhyperforin while the expression of
HpPKS2 showed correlation with the concentrations of hypericins and pseudohypericins in
H. perforatum tissues. |
| doi_str_mv | 10.1016/j.jplph.2007.04.008 |
| format | Article |
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HpPKS1 and
HpPKS2, were isolated from
Hypericum perforatum. The full-length
HpPKS1 was 1573
bp containing an open reading frame (ORF) of 1161
bp encoding for a 386 amino acid protein. The full-length cDNA of
HpPKS2 was 1559
bp with an ORF of 1182
bp encoding for a 393 amino acid protein. The highly conserved catalytic amino acid residues common to plant-specific PKSs were preserved in both genes.
HpPKS1 and
HpPKS2 exhibited distinct tissue-specific expression patterns in
H. perforatum. The
HpPKS1 expression was highest in flower buds and lowest in root tissues. The expression of
HpPKS2 was found to be high in flower buds and leaf margins and low in leaf interior parts, stems and roots. The expression of the
HpPKS1 was found to correlate with the concentrations of hyperforin and adhyperforin while the expression of
HpPKS2 showed correlation with the concentrations of hypericins and pseudohypericins in
H. perforatum tissues.</description><identifier>ISSN: 0176-1617</identifier><identifier>EISSN: 1618-1328</identifier><identifier>DOI: 10.1016/j.jplph.2007.04.008</identifier><identifier>PMID: 17931742</identifier><identifier>CODEN: JPPHEY</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Agronomy. Soil science and plant productions ; Amino Acid Sequence ; amino acid sequences ; Biological and medical sciences ; Biosynthesis ; carbon-carbon bond ligases ; Classical and quantitative genetics. Population genetics. Molecular genetics ; Cloning, Molecular ; complementary DNA ; DNA, Complementary - genetics ; Flowers ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; Gene Expression Regulation, Enzymologic - physiology ; Gene Expression Regulation, Plant - physiology ; Generalities. Genetics. Plant material ; Genetics and breeding of economic plants ; Hyperforin ; Hypericin ; Hypericum - enzymology ; Hypericum - genetics ; Hypericum perforatum ; molecular cloning ; Molecular genetics ; Molecular Sequence Data ; Plant Leaves ; Polyketide synthase ; Polyketide Synthases - genetics ; Polyketide Synthases - metabolism ; pseudohypericins ; sequence analysis ; St. John's wort ( Hypericum perforatum)</subject><ispartof>Journal of plant physiology, 2008-01, Vol.165 (10), p.1079-1086</ispartof><rights>2007 Elsevier GmbH</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-b4bcf0e0aac2b72bd2ffee92e2a02fde332c7edd9fa47a69f628dd320acd62e23</citedby><cites>FETCH-LOGICAL-c442t-b4bcf0e0aac2b72bd2ffee92e2a02fde332c7edd9fa47a69f628dd320acd62e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0176161707002271$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20488081$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17931742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karppinen, Katja</creatorcontrib><creatorcontrib>Hohtola, Anja</creatorcontrib><title>Molecular cloning and tissue-specific expression of two cDNAs encoding polyketide synthases from Hypericum perforatum</title><title>Journal of plant physiology</title><addtitle>J Plant Physiol</addtitle><description>Two previously uncharacterized cDNAs encoding for polyketide synthases (PKSs), designated as
HpPKS1 and
HpPKS2, were isolated from
Hypericum perforatum. The full-length
HpPKS1 was 1573
bp containing an open reading frame (ORF) of 1161
bp encoding for a 386 amino acid protein. The full-length cDNA of
HpPKS2 was 1559
bp with an ORF of 1182
bp encoding for a 393 amino acid protein. The highly conserved catalytic amino acid residues common to plant-specific PKSs were preserved in both genes.
HpPKS1 and
HpPKS2 exhibited distinct tissue-specific expression patterns in
H. perforatum. The
HpPKS1 expression was highest in flower buds and lowest in root tissues. The expression of
HpPKS2 was found to be high in flower buds and leaf margins and low in leaf interior parts, stems and roots. The expression of the
HpPKS1 was found to correlate with the concentrations of hyperforin and adhyperforin while the expression of
HpPKS2 showed correlation with the concentrations of hypericins and pseudohypericins in
H. perforatum tissues.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>carbon-carbon bond ligases</subject><subject>Classical and quantitative genetics. Population genetics. Molecular genetics</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>DNA, Complementary - genetics</subject><subject>Flowers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genetics and breeding of economic plants</subject><subject>Hyperforin</subject><subject>Hypericin</subject><subject>Hypericum - enzymology</subject><subject>Hypericum - genetics</subject><subject>Hypericum perforatum</subject><subject>molecular cloning</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Plant Leaves</subject><subject>Polyketide synthase</subject><subject>Polyketide Synthases - genetics</subject><subject>Polyketide Synthases - metabolism</subject><subject>pseudohypericins</subject><subject>sequence analysis</subject><subject>St. John's wort ( Hypericum perforatum)</subject><issn>0176-1617</issn><issn>1618-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvCL0ACX-gtYfxB4hw4VOWjSAUO0LPl2OPWSxIHOwH23-NlV3CD02is531lzUPIEwY1A9a82NbbeZjvag7Q1iBrAHWPbFjDVMUEV_fJBljbVOWhPSGnOW-h7C-VeEhOWNsJ1kq-IeuHOKBdB5OoHeIUpltqJkeXkPOKVZ7RBh8sxZ9zwpxDnGj0dPkRqX398SJTnGx0-9Ach91XXIJDmnfTcmcyZupTHOnVbsYU7DrSMn1MZlnHR-SBN0PGx8d5Rm7evvlyeVVdf3r3_vLiurJS8qXqZW89IBhjed_y3nHvETuO3AD3DoXgtkXnOm9ka5rON1w5JzgY65pCiTNyfuidU_y2Yl70GLLFYTATxjXrpuPllEr8F-TQSQ6tLKA4gDbFnBN6PacwmrTTDPRei97q31r0XosGqYuWknp6rF_7Ed3fzNFDAZ4fAZOtGXwykw35D8dBKgWKFe7ZgfMmanObCnPzmQMTAB0DpfZNrw4Elrt-D5h0tqFoQhcS2kW7GP751V955blK</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Karppinen, Katja</creator><creator>Hohtola, Anja</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080101</creationdate><title>Molecular cloning and tissue-specific expression of two cDNAs encoding polyketide synthases from Hypericum perforatum</title><author>Karppinen, Katja ; Hohtola, Anja</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-b4bcf0e0aac2b72bd2ffee92e2a02fde332c7edd9fa47a69f628dd320acd62e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>carbon-carbon bond ligases</topic><topic>Classical and quantitative genetics. Population genetics. Molecular genetics</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>DNA, Complementary - genetics</topic><topic>Flowers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genetics and breeding of economic plants</topic><topic>Hyperforin</topic><topic>Hypericin</topic><topic>Hypericum - enzymology</topic><topic>Hypericum - genetics</topic><topic>Hypericum perforatum</topic><topic>molecular cloning</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Plant Leaves</topic><topic>Polyketide synthase</topic><topic>Polyketide Synthases - genetics</topic><topic>Polyketide Synthases - metabolism</topic><topic>pseudohypericins</topic><topic>sequence analysis</topic><topic>St. John's wort ( Hypericum perforatum)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karppinen, Katja</creatorcontrib><creatorcontrib>Hohtola, Anja</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karppinen, Katja</au><au>Hohtola, Anja</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and tissue-specific expression of two cDNAs encoding polyketide synthases from Hypericum perforatum</atitle><jtitle>Journal of plant physiology</jtitle><addtitle>J Plant Physiol</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>165</volume><issue>10</issue><spage>1079</spage><epage>1086</epage><pages>1079-1086</pages><issn>0176-1617</issn><eissn>1618-1328</eissn><coden>JPPHEY</coden><abstract>Two previously uncharacterized cDNAs encoding for polyketide synthases (PKSs), designated as
HpPKS1 and
HpPKS2, were isolated from
Hypericum perforatum. The full-length
HpPKS1 was 1573
bp containing an open reading frame (ORF) of 1161
bp encoding for a 386 amino acid protein. The full-length cDNA of
HpPKS2 was 1559
bp with an ORF of 1182
bp encoding for a 393 amino acid protein. The highly conserved catalytic amino acid residues common to plant-specific PKSs were preserved in both genes.
HpPKS1 and
HpPKS2 exhibited distinct tissue-specific expression patterns in
H. perforatum. The
HpPKS1 expression was highest in flower buds and lowest in root tissues. The expression of
HpPKS2 was found to be high in flower buds and leaf margins and low in leaf interior parts, stems and roots. The expression of the
HpPKS1 was found to correlate with the concentrations of hyperforin and adhyperforin while the expression of
HpPKS2 showed correlation with the concentrations of hypericins and pseudohypericins in
H. perforatum tissues.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>17931742</pmid><doi>10.1016/j.jplph.2007.04.008</doi><tpages>8</tpages></addata></record> |
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| issn | 0176-1617 1618-1328 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Agronomy. Soil science and plant productions Amino Acid Sequence amino acid sequences Biological and medical sciences Biosynthesis carbon-carbon bond ligases Classical and quantitative genetics. Population genetics. Molecular genetics Cloning, Molecular complementary DNA DNA, Complementary - genetics Flowers Fundamental and applied biological sciences. Psychology gene expression regulation Gene Expression Regulation, Enzymologic - physiology Gene Expression Regulation, Plant - physiology Generalities. Genetics. Plant material Genetics and breeding of economic plants Hyperforin Hypericin Hypericum - enzymology Hypericum - genetics Hypericum perforatum molecular cloning Molecular genetics Molecular Sequence Data Plant Leaves Polyketide synthase Polyketide Synthases - genetics Polyketide Synthases - metabolism pseudohypericins sequence analysis St. John's wort ( Hypericum perforatum) |
| title | Molecular cloning and tissue-specific expression of two cDNAs encoding polyketide synthases from Hypericum perforatum |
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