Immunohistochemical, in situ hybridization and biochemical studies on endogenous cellulase of Corbicula japonica
We previously reported on the endogenous cellulase gene of Corbicula japonica, CjCel9A. In this study, the tissue localization of the mRNA and translated products of CjCel9A was investigated in order to understand how this gene is physiologically involved in cellulose decomposition by C. japonica. A...
Gespeichert in:
Veröffentlicht in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2008-06, Vol.150 (2), p.216-221 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | We previously reported on the endogenous cellulase gene of
Corbicula japonica,
CjCel9A. In this study, the tissue localization of the mRNA and translated products of
CjCel9A was investigated in order to understand how this gene is physiologically involved in cellulose decomposition by
C. japonica. Antiserum against recombinant
CjCel9A protein was prepared. Multiple bands were observed mainly on western blot analysis of the crystalline style, and the band sizes partially corresponded to the active bands detected using zymographic analysis.
In situ hybridization and immunohistochemical analyses clarified the exclusive production and secretion of this cellulase by the secretory cells localized in the epithelium of the digestive tubules in the digestive gland. These data strongly support our previous assumption that the endogenous cellulase of
C. japonica is produced in the digestive gland and transported to the crystalline style to act as a component of its cellulolytic activity. |
---|---|
ISSN: | 1096-4959 1879-1107 |
DOI: | 10.1016/j.cbpb.2008.03.004 |