Aqueous chromatographic system for separation of biomolecules using thermoresponsive polymer modified stationary phase
We have investigated a new method for HPLC using packing materials modified with a functional polymer, such as thermoresponsive poly( N-isopropylacrylamide) (PNIPAAm). PNIPAAm-modified silica exhibits temperature-controlled hydrophilic–hydrophobic surface property changes in aqueous systems. Tempera...
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| Veröffentlicht in: | Journal of Chromatography A 2008-05, Vol.1191 (1), p.157-161 |
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| container_title | Journal of Chromatography A |
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| creator | Kanazawa, Hideko Nishikawa, Mayumi Mizutani, Aya Sakamoto, Chikako Morita-Murase, Yuko Nagata, Yoshiko Kikuchi, Akihiko Okano, Teruo |
| description | We have investigated a new method for HPLC using packing materials modified with a functional polymer, such as thermoresponsive poly(
N-isopropylacrylamide) (PNIPAAm). PNIPAAm-modified silica exhibits temperature-controlled hydrophilic–hydrophobic surface property changes in aqueous systems. Temperature-responsive chromatography is performed with an aqueous mobile phase without using an organic solvent. We designed ternary copolymers of NIPAAm introduced 2-(dimethyl-amino) ethyl methacrylate (DMAEMA) as a cationic monomer and butyl methacrylate (BMA) as a hydrophobic monomer. A cationic thermoresponsive hydrogel grafted surface would produce an alterable stationary phase with both thermally regulated hydrophobicity and charge density for separation of bioactive compounds. In this study, we achieved successful separation of lysozyme without the loss of bioactivity by temperature-responsive chromatography. The electrostatic and hydrophobic interactions could be modulated simultaneously with the temperature in an aqueous mobile phase, thus the separation system would have potential applications in the separation of biomolecules. |
| doi_str_mv | 10.1016/j.chroma.2008.01.056 |
| format | Article |
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N-isopropylacrylamide) (PNIPAAm). PNIPAAm-modified silica exhibits temperature-controlled hydrophilic–hydrophobic surface property changes in aqueous systems. Temperature-responsive chromatography is performed with an aqueous mobile phase without using an organic solvent. We designed ternary copolymers of NIPAAm introduced 2-(dimethyl-amino) ethyl methacrylate (DMAEMA) as a cationic monomer and butyl methacrylate (BMA) as a hydrophobic monomer. A cationic thermoresponsive hydrogel grafted surface would produce an alterable stationary phase with both thermally regulated hydrophobicity and charge density for separation of bioactive compounds. In this study, we achieved successful separation of lysozyme without the loss of bioactivity by temperature-responsive chromatography. The electrostatic and hydrophobic interactions could be modulated simultaneously with the temperature in an aqueous mobile phase, thus the separation system would have potential applications in the separation of biomolecules.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2008.01.056</identifier><identifier>PMID: 18289554</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acrylamides - chemistry ; Acrylic Resins ; Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; Human serum albumin ; Humans ; Lysozyme ; Muramidase - isolation & purification ; Peptide ; Poly( N-isopropylacrylamide) (PNIPAAm) ; Polymers - chemistry ; Protein ; Proteins - isolation & purification ; Serum Albumin - isolation & purification ; Temperature ; Temperature-responsive chromatography</subject><ispartof>Journal of Chromatography A, 2008-05, Vol.1191 (1), p.157-161</ispartof><rights>2008 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-c10399efad87a7a6ba52b98c77c7321742878c4e291008461e8187e3f714c6493</citedby><cites>FETCH-LOGICAL-c450t-c10399efad87a7a6ba52b98c77c7321742878c4e291008461e8187e3f714c6493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chroma.2008.01.056$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18289554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kanazawa, Hideko</creatorcontrib><creatorcontrib>Nishikawa, Mayumi</creatorcontrib><creatorcontrib>Mizutani, Aya</creatorcontrib><creatorcontrib>Sakamoto, Chikako</creatorcontrib><creatorcontrib>Morita-Murase, Yuko</creatorcontrib><creatorcontrib>Nagata, Yoshiko</creatorcontrib><creatorcontrib>Kikuchi, Akihiko</creatorcontrib><creatorcontrib>Okano, Teruo</creatorcontrib><title>Aqueous chromatographic system for separation of biomolecules using thermoresponsive polymer modified stationary phase</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>We have investigated a new method for HPLC using packing materials modified with a functional polymer, such as thermoresponsive poly(
N-isopropylacrylamide) (PNIPAAm). PNIPAAm-modified silica exhibits temperature-controlled hydrophilic–hydrophobic surface property changes in aqueous systems. Temperature-responsive chromatography is performed with an aqueous mobile phase without using an organic solvent. We designed ternary copolymers of NIPAAm introduced 2-(dimethyl-amino) ethyl methacrylate (DMAEMA) as a cationic monomer and butyl methacrylate (BMA) as a hydrophobic monomer. A cationic thermoresponsive hydrogel grafted surface would produce an alterable stationary phase with both thermally regulated hydrophobicity and charge density for separation of bioactive compounds. In this study, we achieved successful separation of lysozyme without the loss of bioactivity by temperature-responsive chromatography. The electrostatic and hydrophobic interactions could be modulated simultaneously with the temperature in an aqueous mobile phase, thus the separation system would have potential applications in the separation of biomolecules.</description><subject>Acrylamides - chemistry</subject><subject>Acrylic Resins</subject><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Human serum albumin</subject><subject>Humans</subject><subject>Lysozyme</subject><subject>Muramidase - isolation & purification</subject><subject>Peptide</subject><subject>Poly( N-isopropylacrylamide) (PNIPAAm)</subject><subject>Polymers - chemistry</subject><subject>Protein</subject><subject>Proteins - isolation & purification</subject><subject>Serum Albumin - isolation & purification</subject><subject>Temperature</subject><subject>Temperature-responsive chromatography</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1v2zAURTm0SNK0_6BoOXWzSlKUSC4BgiAfBQJ0aDITNPVk0xBFhU8y4H9fqTKQrdNb7j149xDylbOCM17_PBR-n1N0hWBMF4wXrKo_kCvGBN-YWpWX5BPigTGumBIX5JJroU1VyStyvH2bIE1IV8CYdtkN--ApnnCESNuUKcLgshtD6mlq6TakmDrwUwdIJwz9jo57yDFlwCH1GI5Ah9SdImQaUxPaAA3F8V_f5RMd9g7hM_nYug7hy_lek9eH-5e7p83z78dfd7fPGy8rNm48Z6Ux0LpGK6dcvXWV2BrtlfKqFFxJoZX2EoTh83BZc9BcKyhbxaWvpSmvyY-VO-Q0D8XRxoAeus71y2pbG8Ek10tQrkGfE2KG1g45xPlfy5ldHNuDXRXZxbFl3M6O59q3M3_aRmjeS2fBc-D7Gmhdsm6XA9rXP4LxcoYYqcyCuFkTMHs4BsgWfYDeQxMy-NE2Kfz_h78sIp14</recordid><startdate>20080516</startdate><enddate>20080516</enddate><creator>Kanazawa, Hideko</creator><creator>Nishikawa, Mayumi</creator><creator>Mizutani, Aya</creator><creator>Sakamoto, Chikako</creator><creator>Morita-Murase, Yuko</creator><creator>Nagata, Yoshiko</creator><creator>Kikuchi, Akihiko</creator><creator>Okano, Teruo</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080516</creationdate><title>Aqueous chromatographic system for separation of biomolecules using thermoresponsive polymer modified stationary phase</title><author>Kanazawa, Hideko ; Nishikawa, Mayumi ; Mizutani, Aya ; Sakamoto, Chikako ; Morita-Murase, Yuko ; Nagata, Yoshiko ; Kikuchi, Akihiko ; Okano, Teruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-c10399efad87a7a6ba52b98c77c7321742878c4e291008461e8187e3f714c6493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acrylamides - chemistry</topic><topic>Acrylic Resins</topic><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Human serum albumin</topic><topic>Humans</topic><topic>Lysozyme</topic><topic>Muramidase - isolation & purification</topic><topic>Peptide</topic><topic>Poly( N-isopropylacrylamide) (PNIPAAm)</topic><topic>Polymers - chemistry</topic><topic>Protein</topic><topic>Proteins - isolation & purification</topic><topic>Serum Albumin - isolation & purification</topic><topic>Temperature</topic><topic>Temperature-responsive chromatography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kanazawa, Hideko</creatorcontrib><creatorcontrib>Nishikawa, Mayumi</creatorcontrib><creatorcontrib>Mizutani, Aya</creatorcontrib><creatorcontrib>Sakamoto, Chikako</creatorcontrib><creatorcontrib>Morita-Murase, Yuko</creatorcontrib><creatorcontrib>Nagata, Yoshiko</creatorcontrib><creatorcontrib>Kikuchi, Akihiko</creatorcontrib><creatorcontrib>Okano, Teruo</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kanazawa, Hideko</au><au>Nishikawa, Mayumi</au><au>Mizutani, Aya</au><au>Sakamoto, Chikako</au><au>Morita-Murase, Yuko</au><au>Nagata, Yoshiko</au><au>Kikuchi, Akihiko</au><au>Okano, Teruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aqueous chromatographic system for separation of biomolecules using thermoresponsive polymer modified stationary phase</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2008-05-16</date><risdate>2008</risdate><volume>1191</volume><issue>1</issue><spage>157</spage><epage>161</epage><pages>157-161</pages><issn>0021-9673</issn><abstract>We have investigated a new method for HPLC using packing materials modified with a functional polymer, such as thermoresponsive poly(
N-isopropylacrylamide) (PNIPAAm). PNIPAAm-modified silica exhibits temperature-controlled hydrophilic–hydrophobic surface property changes in aqueous systems. Temperature-responsive chromatography is performed with an aqueous mobile phase without using an organic solvent. We designed ternary copolymers of NIPAAm introduced 2-(dimethyl-amino) ethyl methacrylate (DMAEMA) as a cationic monomer and butyl methacrylate (BMA) as a hydrophobic monomer. A cationic thermoresponsive hydrogel grafted surface would produce an alterable stationary phase with both thermally regulated hydrophobicity and charge density for separation of bioactive compounds. In this study, we achieved successful separation of lysozyme without the loss of bioactivity by temperature-responsive chromatography. The electrostatic and hydrophobic interactions could be modulated simultaneously with the temperature in an aqueous mobile phase, thus the separation system would have potential applications in the separation of biomolecules.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18289554</pmid><doi>10.1016/j.chroma.2008.01.056</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acrylamides - chemistry Acrylic Resins Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Human serum albumin Humans Lysozyme Muramidase - isolation & purification Peptide Poly( N-isopropylacrylamide) (PNIPAAm) Polymers - chemistry Protein Proteins - isolation & purification Serum Albumin - isolation & purification Temperature Temperature-responsive chromatography |
| title | Aqueous chromatographic system for separation of biomolecules using thermoresponsive polymer modified stationary phase |
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