Sustained expression of keratinase gene under PxylA and PamyL promoters in the recombinant Bacillus megaterium MS941

The ker gene encoding pre-pro keratinase of Bacillus licheniformis MKU3 was cloned with xylose inducible promoter ( PxylA) or α-amylase promoter ( PamyL) or both in Escherichia coli–Bacillus shuttle vector, pWH1520 generating recombinant plasmids pWHK3, pWAK3 and pWXAK3 respectively. Compared with B...

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Veröffentlicht in:	Bioresource technology 2008-09, Vol.99 (13), p.5528-5537
Hauptverfasser:	Radha, S., Gunasekaran, P.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Amylases - genetics Bacillius megaterium Bacillus - enzymology Bacillus - genetics Bacillus licheniformis Bacillus megaterium Bacillus megaterium - enzymology Bacillus megaterium - genetics Bacterial Proteins - genetics Biological and medical sciences Cloning, Molecular Enzyme Stability Escherichia Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Gram-Negative Bacteria - enzymology Gram-Negative Bacteria - genetics Keratinase PamyL Peptide Hydrolases - genetics Peptide Hydrolases - metabolism Promoter Regions, Genetic PxylA Recombinant Proteins - metabolism
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