Evaluation of a Homogeneous Assay for Measuring LDL-cholesterol in Hyperlipidemic Serum Specimens
Aim: Homogeneous assay reagents for the determination of low-density lipoprotein cholesterol (LDL-C) have been available from several manufacturers. However, there has been considerable controversy due to uncertainty regarding their reactivity with intermediate-density lipoprotein (IDL), which is de...
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| Veröffentlicht in: | Journal of Atherosclerosis and Thrombosis 2008, Vol.15(2), pp.82-86 |
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| creator | Yamashita, Shizuya Nakamura, Mitsuhiro Koizumi, Hiroaki Oku, Hiroyuki Sandoval, Jose C. Tsubakio-Yamamoto, Kazumi Kawase, Miyako Masuda, Daisaku Koseki, Masahiro Matsuura, Fumihiko Shimomura, Iichiro Nishida, Makoto Ishigami, Masato |
| description | Aim: Homogeneous assay reagents for the determination of low-density lipoprotein cholesterol (LDL-C) have been available from several manufacturers. However, there has been considerable controversy due to uncertainty regarding their reactivity with intermediate-density lipoprotein (IDL), which is detected at an especially high frequency in patients with type III hyperlipemia. In this study, we examined the reactivity of a homogeneous assay, Cholestest LDL ® (CT-LDL), with hyperlipemic sera that were classified according to the WHO system. Methods: Sera from 6 normolipidemic and 22 hyperlipidemic patients classified according to the WHO system were used for this study. All serum specimens were fractionated by the ultracentrifugation method of Hatch and Lees, and subjected to lipid and protein measurements. Results: The percent bias of values measured by CT-LDL relative to those determined by the ultracentrifugation method was calculated and compared to the lipid/protein ratios of each lipoprotein fraction. Consequently, the coefficient of correlation between the bias and the Triglyceride/Total cholesterol (TG/TC) ratio in the IDL fraction was 0.742. There were also correlations with the TG/TC ratio and the apo-lipoprotein B/Total Cholesterol ratio in the LDL fraction and in the LDL+IDL fraction, respectively. Conclusion: Further testing will be required in order to know more about the clinical condition of hyperlipidemic patients, since CT-LDL may react differently with some beta lipoproteins having a diverse lipid/protein composition compared to those in normolipidemic specimens. |
| doi_str_mv | 10.5551/jat.E527 |
| format | Article |
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However, there has been considerable controversy due to uncertainty regarding their reactivity with intermediate-density lipoprotein (IDL), which is detected at an especially high frequency in patients with type III hyperlipemia. In this study, we examined the reactivity of a homogeneous assay, Cholestest LDL ® (CT-LDL), with hyperlipemic sera that were classified according to the WHO system. Methods: Sera from 6 normolipidemic and 22 hyperlipidemic patients classified according to the WHO system were used for this study. All serum specimens were fractionated by the ultracentrifugation method of Hatch and Lees, and subjected to lipid and protein measurements. Results: The percent bias of values measured by CT-LDL relative to those determined by the ultracentrifugation method was calculated and compared to the lipid/protein ratios of each lipoprotein fraction. Consequently, the coefficient of correlation between the bias and the Triglyceride/Total cholesterol (TG/TC) ratio in the IDL fraction was 0.742. There were also correlations with the TG/TC ratio and the apo-lipoprotein B/Total Cholesterol ratio in the LDL fraction and in the LDL+IDL fraction, respectively. Conclusion: Further testing will be required in order to know more about the clinical condition of hyperlipidemic patients, since CT-LDL may react differently with some beta lipoproteins having a diverse lipid/protein composition compared to those in normolipidemic specimens.</description><identifier>ISSN: 1340-3478</identifier><identifier>EISSN: 1880-3873</identifier><identifier>DOI: 10.5551/jat.E527</identifier><identifier>PMID: 18385537</identifier><language>eng</language><publisher>Japan: Japan Atherosclerosis Society</publisher><subject>Biological Assay - standards ; Cholesterol - blood ; Cholesterol, LDL - blood ; Homogeneous assay ; Humans ; Hyperlipemia ; Hyperlipidemias - blood ; Hyperlipidemias - diagnosis ; LDL subclasses ; Lipoproteins - blood ; Low-density lipoprotein ; Reproducibility of Results ; Triglycerides - blood ; Ultracentrifugation</subject><ispartof>Journal of Atherosclerosis and Thrombosis, 2008, Vol.15(2), pp.82-86</ispartof><rights>2008 Japan Atherosclerosis Society</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4767-1b8ec67d706d4ac98adedf3301ad0a4800d3b25e21d85b3eddb6778a2d8c1fcf3</citedby><cites>FETCH-LOGICAL-c4767-1b8ec67d706d4ac98adedf3301ad0a4800d3b25e21d85b3eddb6778a2d8c1fcf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18385537$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamashita, Shizuya</creatorcontrib><creatorcontrib>Nakamura, Mitsuhiro</creatorcontrib><creatorcontrib>Koizumi, Hiroaki</creatorcontrib><creatorcontrib>Oku, Hiroyuki</creatorcontrib><creatorcontrib>Sandoval, Jose C.</creatorcontrib><creatorcontrib>Tsubakio-Yamamoto, Kazumi</creatorcontrib><creatorcontrib>Kawase, Miyako</creatorcontrib><creatorcontrib>Masuda, Daisaku</creatorcontrib><creatorcontrib>Koseki, Masahiro</creatorcontrib><creatorcontrib>Matsuura, Fumihiko</creatorcontrib><creatorcontrib>Shimomura, Iichiro</creatorcontrib><creatorcontrib>Nishida, Makoto</creatorcontrib><creatorcontrib>Ishigami, Masato</creatorcontrib><title>Evaluation of a Homogeneous Assay for Measuring LDL-cholesterol in Hyperlipidemic Serum Specimens</title><title>Journal of Atherosclerosis and Thrombosis</title><addtitle>JAT</addtitle><description>Aim: Homogeneous assay reagents for the determination of low-density lipoprotein cholesterol (LDL-C) have been available from several manufacturers. However, there has been considerable controversy due to uncertainty regarding their reactivity with intermediate-density lipoprotein (IDL), which is detected at an especially high frequency in patients with type III hyperlipemia. In this study, we examined the reactivity of a homogeneous assay, Cholestest LDL ® (CT-LDL), with hyperlipemic sera that were classified according to the WHO system. Methods: Sera from 6 normolipidemic and 22 hyperlipidemic patients classified according to the WHO system were used for this study. All serum specimens were fractionated by the ultracentrifugation method of Hatch and Lees, and subjected to lipid and protein measurements. Results: The percent bias of values measured by CT-LDL relative to those determined by the ultracentrifugation method was calculated and compared to the lipid/protein ratios of each lipoprotein fraction. Consequently, the coefficient of correlation between the bias and the Triglyceride/Total cholesterol (TG/TC) ratio in the IDL fraction was 0.742. There were also correlations with the TG/TC ratio and the apo-lipoprotein B/Total Cholesterol ratio in the LDL fraction and in the LDL+IDL fraction, respectively. Conclusion: Further testing will be required in order to know more about the clinical condition of hyperlipidemic patients, since CT-LDL may react differently with some beta lipoproteins having a diverse lipid/protein composition compared to those in normolipidemic specimens.</description><subject>Biological Assay - standards</subject><subject>Cholesterol - blood</subject><subject>Cholesterol, LDL - blood</subject><subject>Homogeneous assay</subject><subject>Humans</subject><subject>Hyperlipemia</subject><subject>Hyperlipidemias - blood</subject><subject>Hyperlipidemias - diagnosis</subject><subject>LDL subclasses</subject><subject>Lipoproteins - blood</subject><subject>Low-density lipoprotein</subject><subject>Reproducibility of Results</subject><subject>Triglycerides - blood</subject><subject>Ultracentrifugation</subject><issn>1340-3478</issn><issn>1880-3873</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFO4zAQhi20iHZZpH2ClU8rLgE7jmP3goSgS1cq4sBytib2pLhK4mAnSH17Ulqxl5mR5tOnmZ-Qn5xdSSn59RaGq6XM1QmZc61ZJrQS36ZZFNNcKD0j31PaMiaElPkZmXEttJRCzQks36EZYfCho6GmQFehDRvsMIyJ3qYEO1qHSB8R0hh9t6Hr-3VmX0ODacAYGuo7utr1GBvfe4ett_QZ49jS5x6tb7FLP8hpDU3Ci2M_Jy9_lv_uVtn66eHv3e1kK1SpMl5ptKVyipWuALvQ4NDVQjAOjkGhGXOiyiXm3GlZCXSuKpXSkDtteW1rcU5-H7x9DG_jdJ5pfbLYNPD5jCkXOcsXqpjAywNoY0gpYm366FuIO8OZ2cdppjjNPs4J_XV0jlWL7j94zG8Cbg7ANg2wwS8A4uBtg58mLk2-L3vj18K-QjTYiQ9iLIiX</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Yamashita, Shizuya</creator><creator>Nakamura, Mitsuhiro</creator><creator>Koizumi, Hiroaki</creator><creator>Oku, Hiroyuki</creator><creator>Sandoval, Jose C.</creator><creator>Tsubakio-Yamamoto, Kazumi</creator><creator>Kawase, Miyako</creator><creator>Masuda, Daisaku</creator><creator>Koseki, Masahiro</creator><creator>Matsuura, Fumihiko</creator><creator>Shimomura, Iichiro</creator><creator>Nishida, Makoto</creator><creator>Ishigami, Masato</creator><general>Japan Atherosclerosis Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Evaluation of a Homogeneous Assay for Measuring LDL-cholesterol in Hyperlipidemic Serum Specimens</title><author>Yamashita, Shizuya ; Nakamura, Mitsuhiro ; Koizumi, Hiroaki ; Oku, Hiroyuki ; Sandoval, Jose C. ; Tsubakio-Yamamoto, Kazumi ; Kawase, Miyako ; Masuda, Daisaku ; Koseki, Masahiro ; Matsuura, Fumihiko ; Shimomura, Iichiro ; Nishida, Makoto ; Ishigami, Masato</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4767-1b8ec67d706d4ac98adedf3301ad0a4800d3b25e21d85b3eddb6778a2d8c1fcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological Assay - standards</topic><topic>Cholesterol - blood</topic><topic>Cholesterol, LDL - blood</topic><topic>Homogeneous assay</topic><topic>Humans</topic><topic>Hyperlipemia</topic><topic>Hyperlipidemias - blood</topic><topic>Hyperlipidemias - diagnosis</topic><topic>LDL subclasses</topic><topic>Lipoproteins - blood</topic><topic>Low-density lipoprotein</topic><topic>Reproducibility of Results</topic><topic>Triglycerides - blood</topic><topic>Ultracentrifugation</topic><toplevel>online_resources</toplevel><creatorcontrib>Yamashita, Shizuya</creatorcontrib><creatorcontrib>Nakamura, Mitsuhiro</creatorcontrib><creatorcontrib>Koizumi, Hiroaki</creatorcontrib><creatorcontrib>Oku, Hiroyuki</creatorcontrib><creatorcontrib>Sandoval, Jose C.</creatorcontrib><creatorcontrib>Tsubakio-Yamamoto, Kazumi</creatorcontrib><creatorcontrib>Kawase, Miyako</creatorcontrib><creatorcontrib>Masuda, Daisaku</creatorcontrib><creatorcontrib>Koseki, Masahiro</creatorcontrib><creatorcontrib>Matsuura, Fumihiko</creatorcontrib><creatorcontrib>Shimomura, Iichiro</creatorcontrib><creatorcontrib>Nishida, Makoto</creatorcontrib><creatorcontrib>Ishigami, Masato</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Atherosclerosis and Thrombosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamashita, Shizuya</au><au>Nakamura, Mitsuhiro</au><au>Koizumi, Hiroaki</au><au>Oku, Hiroyuki</au><au>Sandoval, Jose C.</au><au>Tsubakio-Yamamoto, Kazumi</au><au>Kawase, Miyako</au><au>Masuda, Daisaku</au><au>Koseki, Masahiro</au><au>Matsuura, Fumihiko</au><au>Shimomura, Iichiro</au><au>Nishida, Makoto</au><au>Ishigami, Masato</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a Homogeneous Assay for Measuring LDL-cholesterol in Hyperlipidemic Serum Specimens</atitle><jtitle>Journal of Atherosclerosis and Thrombosis</jtitle><addtitle>JAT</addtitle><date>2008</date><risdate>2008</risdate><volume>15</volume><issue>2</issue><spage>82</spage><epage>86</epage><pages>82-86</pages><issn>1340-3478</issn><eissn>1880-3873</eissn><abstract>Aim: Homogeneous assay reagents for the determination of low-density lipoprotein cholesterol (LDL-C) have been available from several manufacturers. However, there has been considerable controversy due to uncertainty regarding their reactivity with intermediate-density lipoprotein (IDL), which is detected at an especially high frequency in patients with type III hyperlipemia. In this study, we examined the reactivity of a homogeneous assay, Cholestest LDL ® (CT-LDL), with hyperlipemic sera that were classified according to the WHO system. Methods: Sera from 6 normolipidemic and 22 hyperlipidemic patients classified according to the WHO system were used for this study. All serum specimens were fractionated by the ultracentrifugation method of Hatch and Lees, and subjected to lipid and protein measurements. Results: The percent bias of values measured by CT-LDL relative to those determined by the ultracentrifugation method was calculated and compared to the lipid/protein ratios of each lipoprotein fraction. Consequently, the coefficient of correlation between the bias and the Triglyceride/Total cholesterol (TG/TC) ratio in the IDL fraction was 0.742. There were also correlations with the TG/TC ratio and the apo-lipoprotein B/Total Cholesterol ratio in the LDL fraction and in the LDL+IDL fraction, respectively. Conclusion: Further testing will be required in order to know more about the clinical condition of hyperlipidemic patients, since CT-LDL may react differently with some beta lipoproteins having a diverse lipid/protein composition compared to those in normolipidemic specimens.</abstract><cop>Japan</cop><pub>Japan Atherosclerosis Society</pub><pmid>18385537</pmid><doi>10.5551/jat.E527</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological Assay - standards Cholesterol - blood Cholesterol, LDL - blood Homogeneous assay Humans Hyperlipemia Hyperlipidemias - blood Hyperlipidemias - diagnosis LDL subclasses Lipoproteins - blood Low-density lipoprotein Reproducibility of Results Triglycerides - blood Ultracentrifugation |
| title | Evaluation of a Homogeneous Assay for Measuring LDL-cholesterol in Hyperlipidemic Serum Specimens |
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