Development and validation of a method for quantitative determination of econazole nitrate in cream formulation by capillary zone electrophoresis
A simple, fast, inexpensive and reliable capillary zone electrophoresis (CZE) method for the determination of econazole nitrate in cream formulations has been developed and validated. Optimum conditions comprised a pH 2.5 phosphate buffer at 20 mmol L −1 concentration, +30 kV applied voltage in a 31...
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| Veröffentlicht in: | Journal of Chromatography A 2008-05, Vol.1192 (2), p.301-305 |
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| container_title | Journal of Chromatography A |
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| creator | Gaona-Galdos, Angel Arturo Filho, Luiz Antonio Zanolli Tavares, Marina Franco Maggi Aurora-Prado, María Segunda Santoro, Maria Inês Rocha Miritello Kedor-Hackmann, Érika Rosa Maria |
| description | A simple, fast, inexpensive and reliable capillary zone electrophoresis (CZE) method for the determination of econazole nitrate in cream formulations has been developed and validated. Optimum conditions comprised a pH 2.5 phosphate buffer at 20
mmol
L
−1 concentration, +30
kV applied voltage in a 31.5
cm
×
50
μm I.D. capillary. Direct UV detection at 200
nm led to an adequate sensitivity without interference from sample excipients. A single extraction step of the cream sample in hydrochloric acid was performed prior to injection. Imidazole (100
μg
mL
−1) was used as internal standard. Econazole nitrate migrates in approximately 1.2
min. The analytical curve presented a coefficient of correlation of 0.9995. Detection and quantitation limits were 1.85 and 5.62
μg
mL
−1, respectively. Excellent accuracy and precision were obtained. Recoveries varied from 98.1 to 102.5% and intra- and inter-day precisions, calculated as relative standard deviation (RSD), were better than 2.0%. The proposed CZE method presented advantageous performance characteristics and it can be considered suitable for the quality control of econazole nitrate cream formulations. |
| doi_str_mv | 10.1016/j.chroma.2008.03.070 |
| format | Article |
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mmol
L
−1 concentration, +30
kV applied voltage in a 31.5
cm
×
50
μm I.D. capillary. Direct UV detection at 200
nm led to an adequate sensitivity without interference from sample excipients. A single extraction step of the cream sample in hydrochloric acid was performed prior to injection. Imidazole (100
μg
mL
−1) was used as internal standard. Econazole nitrate migrates in approximately 1.2
min. The analytical curve presented a coefficient of correlation of 0.9995. Detection and quantitation limits were 1.85 and 5.62
μg
mL
−1, respectively. Excellent accuracy and precision were obtained. Recoveries varied from 98.1 to 102.5% and intra- and inter-day precisions, calculated as relative standard deviation (RSD), were better than 2.0%. The proposed CZE method presented advantageous performance characteristics and it can be considered suitable for the quality control of econazole nitrate cream formulations.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2008.03.070</identifier><identifier>PMID: 18406411</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Antifungal Agents - analysis ; Antifungals ; Biological and medical sciences ; Capillary zone electrophoresis ; Econazole - analysis ; Econazole nitrate ; Electrophoresis, Capillary ; General pharmacology ; Indicators and Reagents ; Medical sciences ; Ointments - analysis ; Pharmacology. Drug treatments ; Reference Standards ; Reproducibility of Results ; Solutions ; Spectrophotometry, Ultraviolet ; Validation</subject><ispartof>Journal of Chromatography A, 2008-05, Vol.1192 (2), p.301-305</ispartof><rights>2008 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-d2b0876b977ce20ecce4111c8b0424e31e582abf7207ea88a961eb0bb7794c883</citedby><cites>FETCH-LOGICAL-c414t-d2b0876b977ce20ecce4111c8b0424e31e582abf7207ea88a961eb0bb7794c883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chroma.2008.03.070$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20351786$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18406411$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gaona-Galdos, Angel Arturo</creatorcontrib><creatorcontrib>Filho, Luiz Antonio Zanolli</creatorcontrib><creatorcontrib>Tavares, Marina Franco Maggi</creatorcontrib><creatorcontrib>Aurora-Prado, María Segunda</creatorcontrib><creatorcontrib>Santoro, Maria Inês Rocha Miritello</creatorcontrib><creatorcontrib>Kedor-Hackmann, Érika Rosa Maria</creatorcontrib><title>Development and validation of a method for quantitative determination of econazole nitrate in cream formulation by capillary zone electrophoresis</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>A simple, fast, inexpensive and reliable capillary zone electrophoresis (CZE) method for the determination of econazole nitrate in cream formulations has been developed and validated. Optimum conditions comprised a pH 2.5 phosphate buffer at 20
mmol
L
−1 concentration, +30
kV applied voltage in a 31.5
cm
×
50
μm I.D. capillary. Direct UV detection at 200
nm led to an adequate sensitivity without interference from sample excipients. A single extraction step of the cream sample in hydrochloric acid was performed prior to injection. Imidazole (100
μg
mL
−1) was used as internal standard. Econazole nitrate migrates in approximately 1.2
min. The analytical curve presented a coefficient of correlation of 0.9995. Detection and quantitation limits were 1.85 and 5.62
μg
mL
−1, respectively. Excellent accuracy and precision were obtained. Recoveries varied from 98.1 to 102.5% and intra- and inter-day precisions, calculated as relative standard deviation (RSD), were better than 2.0%. The proposed CZE method presented advantageous performance characteristics and it can be considered suitable for the quality control of econazole nitrate cream formulations.</description><subject>Analysis</subject><subject>Antifungal Agents - analysis</subject><subject>Antifungals</subject><subject>Biological and medical sciences</subject><subject>Capillary zone electrophoresis</subject><subject>Econazole - analysis</subject><subject>Econazole nitrate</subject><subject>Electrophoresis, Capillary</subject><subject>General pharmacology</subject><subject>Indicators and Reagents</subject><subject>Medical sciences</subject><subject>Ointments - analysis</subject><subject>Pharmacology. Drug treatments</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Solutions</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Validation</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhnMA0VL4Bwh8gduGseONnQsSKp9SJQ7QszVxJqxXib21nZXaf8E_xqusyo3THPy8M6-fqnrFoebA2_f72u5imLEWALqGpgYFT6pLAME3Xauai-p5SnsArkCJZ9UF1xJayfll9ecTHWkKh5l8ZugHdsTJDZhd8CyMDNlMeRcGNobI7hb02eXyeCQ2UKY4O_-Ikg0eH8JEzLscMRNzntlIOJ_C8zKtZH_PLB7cNGG8Zw_BE6OJbI7hsAuRkksvqqcjTolenudVdfvl86_rb5ubH1-_X3-82VjJZd4Moget2r5TypIAspbKh7jVPUghqeG01QL7UQlQhFpj13Lqoe-V6qTVurmq3q17DzHcLZSymV2yVIp5Cksybce7TvJtAeUK2hhSijSaQ3RzqW84mJN-szerfnPSb6AxRX-JvT7vX_qZhn-hs_sCvD0DmCxOY0RvXXrkBDRbrnRbuDcrN2Iw-DsW5vanAN6UY53UjSrEh5Wg4uvoKJpkHXlLg4vFrRmC-3_Xv_GYtGE</recordid><startdate>20080530</startdate><enddate>20080530</enddate><creator>Gaona-Galdos, Angel Arturo</creator><creator>Filho, Luiz Antonio Zanolli</creator><creator>Tavares, Marina Franco Maggi</creator><creator>Aurora-Prado, María Segunda</creator><creator>Santoro, Maria Inês Rocha Miritello</creator><creator>Kedor-Hackmann, Érika Rosa Maria</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080530</creationdate><title>Development and validation of a method for quantitative determination of econazole nitrate in cream formulation by capillary zone electrophoresis</title><author>Gaona-Galdos, Angel Arturo ; Filho, Luiz Antonio Zanolli ; Tavares, Marina Franco Maggi ; Aurora-Prado, María Segunda ; Santoro, Maria Inês Rocha Miritello ; Kedor-Hackmann, Érika Rosa Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-d2b0876b977ce20ecce4111c8b0424e31e582abf7207ea88a961eb0bb7794c883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Analysis</topic><topic>Antifungal Agents - analysis</topic><topic>Antifungals</topic><topic>Biological and medical sciences</topic><topic>Capillary zone electrophoresis</topic><topic>Econazole - analysis</topic><topic>Econazole nitrate</topic><topic>Electrophoresis, Capillary</topic><topic>General pharmacology</topic><topic>Indicators and Reagents</topic><topic>Medical sciences</topic><topic>Ointments - analysis</topic><topic>Pharmacology. Drug treatments</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Solutions</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Validation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaona-Galdos, Angel Arturo</creatorcontrib><creatorcontrib>Filho, Luiz Antonio Zanolli</creatorcontrib><creatorcontrib>Tavares, Marina Franco Maggi</creatorcontrib><creatorcontrib>Aurora-Prado, María Segunda</creatorcontrib><creatorcontrib>Santoro, Maria Inês Rocha Miritello</creatorcontrib><creatorcontrib>Kedor-Hackmann, Érika Rosa Maria</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaona-Galdos, Angel Arturo</au><au>Filho, Luiz Antonio Zanolli</au><au>Tavares, Marina Franco Maggi</au><au>Aurora-Prado, María Segunda</au><au>Santoro, Maria Inês Rocha Miritello</au><au>Kedor-Hackmann, Érika Rosa Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and validation of a method for quantitative determination of econazole nitrate in cream formulation by capillary zone electrophoresis</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2008-05-30</date><risdate>2008</risdate><volume>1192</volume><issue>2</issue><spage>301</spage><epage>305</epage><pages>301-305</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A simple, fast, inexpensive and reliable capillary zone electrophoresis (CZE) method for the determination of econazole nitrate in cream formulations has been developed and validated. Optimum conditions comprised a pH 2.5 phosphate buffer at 20
mmol
L
−1 concentration, +30
kV applied voltage in a 31.5
cm
×
50
μm I.D. capillary. Direct UV detection at 200
nm led to an adequate sensitivity without interference from sample excipients. A single extraction step of the cream sample in hydrochloric acid was performed prior to injection. Imidazole (100
μg
mL
−1) was used as internal standard. Econazole nitrate migrates in approximately 1.2
min. The analytical curve presented a coefficient of correlation of 0.9995. Detection and quantitation limits were 1.85 and 5.62
μg
mL
−1, respectively. Excellent accuracy and precision were obtained. Recoveries varied from 98.1 to 102.5% and intra- and inter-day precisions, calculated as relative standard deviation (RSD), were better than 2.0%. The proposed CZE method presented advantageous performance characteristics and it can be considered suitable for the quality control of econazole nitrate cream formulations.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18406411</pmid><doi>10.1016/j.chroma.2008.03.070</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis Antifungal Agents - analysis Antifungals Biological and medical sciences Capillary zone electrophoresis Econazole - analysis Econazole nitrate Electrophoresis, Capillary General pharmacology Indicators and Reagents Medical sciences Ointments - analysis Pharmacology. Drug treatments Reference Standards Reproducibility of Results Solutions Spectrophotometry, Ultraviolet Validation |
| title | Development and validation of a method for quantitative determination of econazole nitrate in cream formulation by capillary zone electrophoresis |
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