Berberine Induced Down-regulation of Matrix Metalloproteinase-1, -2 and -9 in Human Gastric Cancer Cells (SNU-5) In Vitro
Berberine, a yellow benzylisoquinoline alkaloid, is a constituent of Coptis chines and is commonly used in Chinese herbal medicine for patients with gastrointestinal disorders. The pharmacological effects of berberine include anti-inflammation, antidiarrhetic, antimalarial, and even antimicrobial ac...
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| Veröffentlicht in: | In vivo (Athens) 2008-03, Vol.22 (2), p.223-230 |
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| creator | Lin, Jing-Pin Yang, Jai-Sing Wu, Chih-Chung Lin, Song-Shei Hsieh, Wen-Tsong Lin, Meng-Liang Yu, Fu-Shun Yu, Chun-Shu Chen, Guang-Wei Chang, Yung-Hsien Chung, Jing-Gung |
| description | Berberine, a yellow benzylisoquinoline alkaloid, is a constituent of Coptis chines and is commonly used in Chinese herbal
medicine for patients with gastrointestinal disorders. The pharmacological effects of berberine include anti-inflammation,
antidiarrhetic, antimalarial, and even antimicrobial activities. However, its mechanism of action on the cell migration of
human gastric cancer SNU-5 cells is not fully understood. The effects of berberine on the percentage of viable cells were
examined first and it was found that berberine induced dose-dependent inhibition in human gastric cancer SNU-5 cells. The
effect of berberine on the levels of reactive oxygen species (ROS) and matrix metalloproteinase-1, -2, -7 and -9 was then
examined using Western blotting and the results showed that berberine induced ROS production for up to 6 hours of incubation.
It was also found that berberine induced down-regulation of MMP-1 -2, and -9 but did not affect the level of MMP-7. The mRNA
levels of MMPs in SNU-5 cells after treatment with berberine for 24 hours were investigated using a polymerase chain reaction
and the results showed that berberine inhibited the gene expression of MMP-1, -2 and -9 in human SNU-5 cells but it did not
affect MMP-7. In conclusion, berberine appears to exert its anticancer properties by inducing ROS production and prevention
of cell migration via inhibition of the gene expression of MMP-1, -2 and -9 in human gastric cancer SNU-5 cancer cells. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69196282</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69196282</sourcerecordid><originalsourceid>FETCH-LOGICAL-h238t-f5a6e1f64f89fc96a0ccc56ac295c78f7a7daa3430ca5d3121a9afbebeba9f703</originalsourceid><addsrcrecordid>eNo10E1LAzEQBuBFFFs__oLkIKJgIMl-5qirtgU_Dlrxtkyzkzaym63JrtV_b6CV9zCXh5nh3YvGPJec5mki96MxE2lBi5R_jKIj7z8Zy3LGxGE04kWSFQnLx9HvLboFOmORzGw9KKzJXbex1OFyaKA3nSWdJk_QO_NDnrCHpunWruvRWPBI-TWhgoCtCZXEWDIdWrBkAj54RUqwCh0psWk8uXx9ntP0Kpwh76Z33Ul0oKHxeLqbx9H84f6tnNLHl8msvHmkKxEXPdUpZMh1luhCaiUzYEqpNAMlZKryQueQ1wBxEjMFaR1zwUGCXmAISJ2z-Di62O4Nb38N6PuqNV6Fl8BiN_gqk1xmohABnu3gsGixrtbOtOB-q_-yAjjfgpVZrjbGYeXb0EfgcWW-hahCRBz_AYOCcxo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69196282</pqid></control><display><type>article</type><title>Berberine Induced Down-regulation of Matrix Metalloproteinase-1, -2 and -9 in Human Gastric Cancer Cells (SNU-5) In Vitro</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Lin, Jing-Pin ; Yang, Jai-Sing ; Wu, Chih-Chung ; Lin, Song-Shei ; Hsieh, Wen-Tsong ; Lin, Meng-Liang ; Yu, Fu-Shun ; Yu, Chun-Shu ; Chen, Guang-Wei ; Chang, Yung-Hsien ; Chung, Jing-Gung</creator><creatorcontrib>Lin, Jing-Pin ; Yang, Jai-Sing ; Wu, Chih-Chung ; Lin, Song-Shei ; Hsieh, Wen-Tsong ; Lin, Meng-Liang ; Yu, Fu-Shun ; Yu, Chun-Shu ; Chen, Guang-Wei ; Chang, Yung-Hsien ; Chung, Jing-Gung</creatorcontrib><description>Berberine, a yellow benzylisoquinoline alkaloid, is a constituent of Coptis chines and is commonly used in Chinese herbal
medicine for patients with gastrointestinal disorders. The pharmacological effects of berberine include anti-inflammation,
antidiarrhetic, antimalarial, and even antimicrobial activities. However, its mechanism of action on the cell migration of
human gastric cancer SNU-5 cells is not fully understood. The effects of berberine on the percentage of viable cells were
examined first and it was found that berberine induced dose-dependent inhibition in human gastric cancer SNU-5 cells. The
effect of berberine on the levels of reactive oxygen species (ROS) and matrix metalloproteinase-1, -2, -7 and -9 was then
examined using Western blotting and the results showed that berberine induced ROS production for up to 6 hours of incubation.
It was also found that berberine induced down-regulation of MMP-1 -2, and -9 but did not affect the level of MMP-7. The mRNA
levels of MMPs in SNU-5 cells after treatment with berberine for 24 hours were investigated using a polymerase chain reaction
and the results showed that berberine inhibited the gene expression of MMP-1, -2 and -9 in human SNU-5 cells but it did not
affect MMP-7. In conclusion, berberine appears to exert its anticancer properties by inducing ROS production and prevention
of cell migration via inhibition of the gene expression of MMP-1, -2 and -9 in human gastric cancer SNU-5 cancer cells.</description><identifier>ISSN: 0258-851X</identifier><identifier>EISSN: 1791-7549</identifier><identifier>PMID: 18468407</identifier><language>eng</language><publisher>Greece: International Institute of Anticancer Research</publisher><subject>Berberine - pharmacology ; Cell Line, Tumor ; Cell Survival - drug effects ; Dose-Response Relationship, Drug ; Down-Regulation ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Neoplastic ; Humans ; In Vitro Techniques ; Matrix Metalloproteinase 1 - metabolism ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 9 - metabolism ; RNA, Messenger - metabolism ; Statistics as Topic ; Stomach Neoplasms - drug therapy ; Stomach Neoplasms - pathology</subject><ispartof>In vivo (Athens), 2008-03, Vol.22 (2), p.223-230</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18468407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Jing-Pin</creatorcontrib><creatorcontrib>Yang, Jai-Sing</creatorcontrib><creatorcontrib>Wu, Chih-Chung</creatorcontrib><creatorcontrib>Lin, Song-Shei</creatorcontrib><creatorcontrib>Hsieh, Wen-Tsong</creatorcontrib><creatorcontrib>Lin, Meng-Liang</creatorcontrib><creatorcontrib>Yu, Fu-Shun</creatorcontrib><creatorcontrib>Yu, Chun-Shu</creatorcontrib><creatorcontrib>Chen, Guang-Wei</creatorcontrib><creatorcontrib>Chang, Yung-Hsien</creatorcontrib><creatorcontrib>Chung, Jing-Gung</creatorcontrib><title>Berberine Induced Down-regulation of Matrix Metalloproteinase-1, -2 and -9 in Human Gastric Cancer Cells (SNU-5) In Vitro</title><title>In vivo (Athens)</title><addtitle>In Vivo</addtitle><description>Berberine, a yellow benzylisoquinoline alkaloid, is a constituent of Coptis chines and is commonly used in Chinese herbal
medicine for patients with gastrointestinal disorders. The pharmacological effects of berberine include anti-inflammation,
antidiarrhetic, antimalarial, and even antimicrobial activities. However, its mechanism of action on the cell migration of
human gastric cancer SNU-5 cells is not fully understood. The effects of berberine on the percentage of viable cells were
examined first and it was found that berberine induced dose-dependent inhibition in human gastric cancer SNU-5 cells. The
effect of berberine on the levels of reactive oxygen species (ROS) and matrix metalloproteinase-1, -2, -7 and -9 was then
examined using Western blotting and the results showed that berberine induced ROS production for up to 6 hours of incubation.
It was also found that berberine induced down-regulation of MMP-1 -2, and -9 but did not affect the level of MMP-7. The mRNA
levels of MMPs in SNU-5 cells after treatment with berberine for 24 hours were investigated using a polymerase chain reaction
and the results showed that berberine inhibited the gene expression of MMP-1, -2 and -9 in human SNU-5 cells but it did not
affect MMP-7. In conclusion, berberine appears to exert its anticancer properties by inducing ROS production and prevention
of cell migration via inhibition of the gene expression of MMP-1, -2 and -9 in human gastric cancer SNU-5 cancer cells.</description><subject>Berberine - pharmacology</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Down-Regulation</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Matrix Metalloproteinase 1 - metabolism</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Statistics as Topic</subject><subject>Stomach Neoplasms - drug therapy</subject><subject>Stomach Neoplasms - pathology</subject><issn>0258-851X</issn><issn>1791-7549</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo10E1LAzEQBuBFFFs__oLkIKJgIMl-5qirtgU_Dlrxtkyzkzaym63JrtV_b6CV9zCXh5nh3YvGPJec5mki96MxE2lBi5R_jKIj7z8Zy3LGxGE04kWSFQnLx9HvLboFOmORzGw9KKzJXbex1OFyaKA3nSWdJk_QO_NDnrCHpunWruvRWPBI-TWhgoCtCZXEWDIdWrBkAj54RUqwCh0psWk8uXx9ntP0Kpwh76Z33Ul0oKHxeLqbx9H84f6tnNLHl8msvHmkKxEXPdUpZMh1luhCaiUzYEqpNAMlZKryQueQ1wBxEjMFaR1zwUGCXmAISJ2z-Di62O4Nb38N6PuqNV6Fl8BiN_gqk1xmohABnu3gsGixrtbOtOB-q_-yAjjfgpVZrjbGYeXb0EfgcWW-hahCRBz_AYOCcxo</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Lin, Jing-Pin</creator><creator>Yang, Jai-Sing</creator><creator>Wu, Chih-Chung</creator><creator>Lin, Song-Shei</creator><creator>Hsieh, Wen-Tsong</creator><creator>Lin, Meng-Liang</creator><creator>Yu, Fu-Shun</creator><creator>Yu, Chun-Shu</creator><creator>Chen, Guang-Wei</creator><creator>Chang, Yung-Hsien</creator><creator>Chung, Jing-Gung</creator><general>International Institute of Anticancer Research</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20080301</creationdate><title>Berberine Induced Down-regulation of Matrix Metalloproteinase-1, -2 and -9 in Human Gastric Cancer Cells (SNU-5) In Vitro</title><author>Lin, Jing-Pin ; Yang, Jai-Sing ; Wu, Chih-Chung ; Lin, Song-Shei ; Hsieh, Wen-Tsong ; Lin, Meng-Liang ; Yu, Fu-Shun ; Yu, Chun-Shu ; Chen, Guang-Wei ; Chang, Yung-Hsien ; Chung, Jing-Gung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h238t-f5a6e1f64f89fc96a0ccc56ac295c78f7a7daa3430ca5d3121a9afbebeba9f703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Berberine - pharmacology</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Down-Regulation</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Matrix Metalloproteinase 1 - metabolism</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Statistics as Topic</topic><topic>Stomach Neoplasms - drug therapy</topic><topic>Stomach Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Jing-Pin</creatorcontrib><creatorcontrib>Yang, Jai-Sing</creatorcontrib><creatorcontrib>Wu, Chih-Chung</creatorcontrib><creatorcontrib>Lin, Song-Shei</creatorcontrib><creatorcontrib>Hsieh, Wen-Tsong</creatorcontrib><creatorcontrib>Lin, Meng-Liang</creatorcontrib><creatorcontrib>Yu, Fu-Shun</creatorcontrib><creatorcontrib>Yu, Chun-Shu</creatorcontrib><creatorcontrib>Chen, Guang-Wei</creatorcontrib><creatorcontrib>Chang, Yung-Hsien</creatorcontrib><creatorcontrib>Chung, Jing-Gung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>In vivo (Athens)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Jing-Pin</au><au>Yang, Jai-Sing</au><au>Wu, Chih-Chung</au><au>Lin, Song-Shei</au><au>Hsieh, Wen-Tsong</au><au>Lin, Meng-Liang</au><au>Yu, Fu-Shun</au><au>Yu, Chun-Shu</au><au>Chen, Guang-Wei</au><au>Chang, Yung-Hsien</au><au>Chung, Jing-Gung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Berberine Induced Down-regulation of Matrix Metalloproteinase-1, -2 and -9 in Human Gastric Cancer Cells (SNU-5) In Vitro</atitle><jtitle>In vivo (Athens)</jtitle><addtitle>In Vivo</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>22</volume><issue>2</issue><spage>223</spage><epage>230</epage><pages>223-230</pages><issn>0258-851X</issn><eissn>1791-7549</eissn><abstract>Berberine, a yellow benzylisoquinoline alkaloid, is a constituent of Coptis chines and is commonly used in Chinese herbal
medicine for patients with gastrointestinal disorders. The pharmacological effects of berberine include anti-inflammation,
antidiarrhetic, antimalarial, and even antimicrobial activities. However, its mechanism of action on the cell migration of
human gastric cancer SNU-5 cells is not fully understood. The effects of berberine on the percentage of viable cells were
examined first and it was found that berberine induced dose-dependent inhibition in human gastric cancer SNU-5 cells. The
effect of berberine on the levels of reactive oxygen species (ROS) and matrix metalloproteinase-1, -2, -7 and -9 was then
examined using Western blotting and the results showed that berberine induced ROS production for up to 6 hours of incubation.
It was also found that berberine induced down-regulation of MMP-1 -2, and -9 but did not affect the level of MMP-7. The mRNA
levels of MMPs in SNU-5 cells after treatment with berberine for 24 hours were investigated using a polymerase chain reaction
and the results showed that berberine inhibited the gene expression of MMP-1, -2 and -9 in human SNU-5 cells but it did not
affect MMP-7. In conclusion, berberine appears to exert its anticancer properties by inducing ROS production and prevention
of cell migration via inhibition of the gene expression of MMP-1, -2 and -9 in human gastric cancer SNU-5 cancer cells.</abstract><cop>Greece</cop><pub>International Institute of Anticancer Research</pub><pmid>18468407</pmid><tpages>8</tpages></addata></record> |
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| subjects | Berberine - pharmacology Cell Line, Tumor Cell Survival - drug effects Dose-Response Relationship, Drug Down-Regulation Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans In Vitro Techniques Matrix Metalloproteinase 1 - metabolism Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 9 - metabolism RNA, Messenger - metabolism Statistics as Topic Stomach Neoplasms - drug therapy Stomach Neoplasms - pathology |
| title | Berberine Induced Down-regulation of Matrix Metalloproteinase-1, -2 and -9 in Human Gastric Cancer Cells (SNU-5) In Vitro |
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