Synthesis of rotavirus-like particles in insect cells: Comparative and quantitative analysis

When the three major structural proteins, VP2, VP6, and VP7, of rotavirus are co‐expressed in insect cells infected with recombinant baculoviruses, they self‐assemble into triple‐layered virus‐like particles (VLPs) that are similar in morphology to native rotavirus. In order to establish the most fa...

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Veröffentlicht in:	Biotechnology and bioengineering 1998-11, Vol.60 (3), p.369-374
Hauptverfasser:	Jiang, Baoming, Barniak, Vicki, Smith, Robert P., Sharma, Ritu, Corsaro, Bartholomew, Hu, Branda, Madore, H. Paul
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cell culture Animal cells Animals Antigens, Viral Baculoviridae - genetics Biological and medical sciences Biotechnology Capsid - biosynthesis Capsid - genetics Capsid - isolation & purification Capsid Proteins Cell Culture Techniques - methods Cell Line Culture Media, Serum-Free Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Genetic Vectors Growth kinetics insect cell lines Insecta Kinetics Methods. Procedures. Technologies Noctuidae Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Rotavirus Rotavirus - genetics Spodoptera Spodoptera frugiperda Time Factors Transfection - methods virus-like particle Viruses
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