Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones

The directed evolution of an esterase from Pseudomonas fluorescens using the mutator strain Epicurian coli XL1‐Red was investigated. Mutants were assayed for their ability to hydrolyze a sterically hindered 3‐hydroxy ester, which can serve as a building block in the synthesis of epothilones. Screeni...

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